RU2004154C1 - Remedy for treating estrosis in sheep - Google Patents

Abstract

Usage: in veterinary medicine, in particular in compositions for the treatment of sheep estrosis. The inventive composition includes the following components, wt.%: Cypermetry 0.5-4, ethanol 23-29, terpinhydrate 0.4-0.6, solvent (nefras or solvent) 19-30, and the rest is propellant. 1 zlf, 3 tpp

Description

The invention relates to agriculture, in particular to veterinary medicine, is used for the treatment of animals affected by endo- and ectoparasites, in particular against sheep ostrosis - larvae of the nasopharyngeal (cavity) gadfly. An aerosol insecticidal composition (Estrosol) is known containing an organophosphorus insecticide, a solvent and a propellant for controlling nasopharyngeal gadfly larvae. 1 The composition of estrosol includes the active substance dimethyldichlorovinyl phosphate (DDVF) - 13.9 ± 0.5%, solvent xylene-7, 5 ± 0.5% and the propellant freon 11 and 12-78.6 ± 3.0% 2.

A disadvantage of estrosol intended for controlling sheep estrosis is the need to repeat the treatment in several cases, the toxicity of organophosphate compounds - DDVF, as well as xylene, which is a part of the known composition, which causes irritation of the nasal mucosa in sheep, as expressed in sneezing and coughing, appearing in the first minutes after treatment.

The closest technical solution selected as a prototype is 0.1% aqueous cypermethrin emulsion (aerosol).

Cypermethrin (C22H1dC12MO3) is a thick, brown mass with a specific faint odor.

Its boiling point is over 270 ° C, its melting point is full of liquid at 60 ° C, solubility at 20 ° C in xylene, toluene, acetone, chloroform, cyclohexane 450, and 0.2 ppm in water.

Medium toxic for warm-blooded. Its LDB for rats when administered orally is 250-300 mg / kg 4.

The disadvantage of cypermethrin (aerosol) is the high consumption of the drug (500 mg / m3) with an efficiency of 100% for the treatment of sheep estrosis, as well as the long term completion of inflammatory processes after the destruction of larvae of the nasopharyngeal gadfly on the mucous membranes of the upper respiratory tract of animals.

The aim of the invention is to reduce the content of the active substance (AI) in the insecticidal composition while maintaining therapeutic efficacy and giving the drug anti-inflammatory PROPERTIES.

The goal is achieved in the composition of Cesol for the treatment of sheep estrosis in that

as the active substance, low-toxicity for warm-blooded cepermetrine is used; as a solvent, solvent and propellant are nephrased. Terpinhydrate, an anti-inflammatory agent and ethanol, is additionally introduced in the following ratio of components, wt.%:

Cypermethrin 0.5-4

Ethanol23-29

Terpinghydrate 0.4 - 0.6

Solvent 19-30

Propellant

For experimental verification of the proposed composition, a number of mixtures of ingredients were prepared (see table 1).

Cesol is prepared by dissolving the calculated amount of cypermethrin in ethanol. Terpinghydrate is added to the resulting mixture. The mixture is thoroughly mixed, a solvent (nephras or solvent) is added to further dissolve cypermethrin. A can is filled with the prepared mixture into which the propellant is dosed.

In table, 1 shows the tested formulations of the proposed composition of Cesol.

The first 5 (1 - 5) formulations with a content of 0.3 - 0.7 wt.% Terpin hydrate, followed by aine 5 (6 - 10) formulations without terpin hydrate additives.

The study of the proposed composition of Cesol was carried out similarly to methods for studying the effectiveness of estrosol (see table 2).

Sheep suspected of having estrosis was subjected to clinical examination. First, animals with characteristic signs of estrosis are selected (the sheep snort, sneeze, thrice heads, serous-mucous fluid is released from the nasal openings).

In order to confirm the diagnosis, 3 sheep were slaughtered and opened. In this case, inflammation of the mucous membrane, larvae of the nasopharyngeal gadfly, and products of inflammation were detected.

The preparation (formulations 1-10) was used in accordance with the well-known Instructions ... in a small room with a volume of 10 m (sealed), with a floor area of 4 m2, 6 sick sheep were placed (on average 1 goal / 0.7 m) . Then aerosols were started at a flow rate of on average 1 g / m3, which corresponded to doses of DV cypermethrin from 1 to 50 mg / m3 (and left the room). Exposure time is 1 hour. Control were 6 sheep affected by extrusion, which were not subjected to treatment with the drug.

After completion of the exposure, the room was thoroughly ventilated.

5 days after treatment, 1 experimental and 1 control sheep were killed daily. The criterion for the effectiveness of the treatment was the absence of nasopharyngeal gadfly larvae in the nasal cavity, labyrinths of the ethmoid bone, frontal sinuses and subthreshold spaces and the absence of signs of inflammation of the respiratory tract mucosa.

Note that compounds 1 and 6 were ineffective, and compounds 5 and 10 showed an effect similar to compounds 4 and 9 (but with a higher consumption of the active substance - cypermethrin). All larvae of the nasopharyngeal gadfly with the use of compositions 2 - 5 died as a result of processing.

At the same time, the use of cezol did not have a negative effect on animals. Thus, a 12-fold increase in the optimal therapeutic rate of DV consumption of cypermethrin (5 mg x 12 60 mg / m3) did not cause toxicosis in sheep (six animals in the experiment).

It should be noted that in all killed control sheep (six goals), active larvae of the nasopharyngeal gadfly, inflammation and ulceration of the mucous membrane of the respiratory tract, as well as copious amounts of mucus and other products of inflammation were found.

The therapeutic effect of the proposed composition against sheep estrosis was tested on sheep farms in the Kotelnikovsky district of the Volgograd region.

The animals were treated in rooms with a volume of 10 m3.

In this case, one group of sheep (6 animals) was treated with aerosols of the proposed composition with the addition of terpinghydrate, another group of sheep (6 animals) was treated with the proposed composition, which did not include terpinghydrate. The third group of sheep (6 animals) was treated with aerosols of estrosol, the fourth group (6 animals) was treated with aerosols of cypermethrin.

The fifth group (6 goals) - sheep affected by extrusion were not subjected to treatment. 5 days after treatment of the sheep with the indicated insecticidal compositions, one control was killed from each group.

a sheep in which the nasal cavity, subthigh spaces, frontal sinuses were opened to account for the larvae of the nasopharyngeal gadfly and to assess the condition of the mucous membranes of 5 membranes.

The therapeutic effect of the proposed compositions in case of extrusion was determined by the percentage of dead larvae and the rate of disappearance of signs of the inflammatory process on 0 mucous membranes in places where larvae of the nasopharyngeal gadfly were located before treatment.

It can be seen from the table that the therapeutic effect against sheep estrosis was 5th highest when using a composition containing terpin hydrate as an additive. In this case, the healing of mucous membranes occurred 3 days faster than with the use of a composition without terpine hydrate and more than 3 days than with estrosol and cypermethrin aerosols. In all 6 control sheep, larvae of the nasopharyngeal gadfly and signs of inflammation of the upper respiratory tract were found.

5 Thus, the proposed composition for the treatment of sheep estrosis tsesol has several advantages over existing, in particular, cypermethrin aerosols.

0 1. Composition of Caesol is more effective against sheep estrosis: Caesol contains on average 4% DV of cypermethrin, estrosol - 12.5% DDVF (with the same average consumption rate), i.e. consumption of DV insecticide

5 is reduced by 3 times.

2. Patients with sheep ostroeomas recover faster after tsezol administration. Due to the administration of terpinghydrate, the treatment time is reduced by 3 days.

0

(56) Brains I.E. Veterinary recipe with the basics of therapy and prevention. Reference book. M .: VO Agropromizdat, 1988, p. 363-365.

5 Bukshtynov V.I. The efficacy of anometrine, neopinamine, cypermethrin, foxime, fozalon, whitefos and ivomek against larvae of the sheep gadfly. Questions of veterinary toxicology, entomology and

0 disinfestation. Proceedings of VNIIVS, Moscow: 1987, pp. 94 - 97.

Table 1

Compositions

Continuation of the table. 1

table 2

Compositions

Continuation of the table. 2

The therapeutic effect of insecticidal compositions against sheep estrosis

Claims (2)

1. A composition for treating sheep estrosis, comprising cypermethrin, a solvent and a propellant, characterized in that it further comprises terpinghydrate and ethanol in the following ratio, wt.%: Table 3 0.5-4.0 23-29 0.4 - 0.6 19-30 Rest 2. The composition according to claim 1, characterized in that nefras or solvent is used as a solvent.