RU2004139259A - METHOD FOR SUPPRESSING REPRODUCTION OF VIRUS DIARRHEA VIRUS - DISEASES OF Mucous cattle in a cell culture - Google Patents

METHOD FOR SUPPRESSING REPRODUCTION OF VIRUS DIARRHEA VIRUS - DISEASES OF Mucous cattle in a cell culture Download PDF

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Publication number
RU2004139259A
RU2004139259A RU2004139259/13A RU2004139259A RU2004139259A RU 2004139259 A RU2004139259 A RU 2004139259A RU 2004139259/13 A RU2004139259/13 A RU 2004139259/13A RU 2004139259 A RU2004139259 A RU 2004139259A RU 2004139259 A RU2004139259 A RU 2004139259A
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RU
Russia
Prior art keywords
virus
cell culture
cattle
mucous
diseases
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RU2004139259/13A
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Russian (ru)
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RU2294742C2 (en
Inventor
Александр Гаврилович Глотов (RU)
Александр Гаврилович Глотов
Тать на Ивановна Глотова (RU)
Татьяна Ивановна Глотова
Original Assignee
Государственное научное учреждение Институт экспериментальной ветеринарии Сибири и Дальнего ВостокаСибирского отделени Россельхозакадемии (ГНУ ИЭВСиДВ СО РАСХН) (RU)
Государственное научное учреждение Институт экспериментальной ветеринарии Сибири и Дальнего Востока Сибирского отделения Россельхозакадемии (ГНУ ИЭВСиДВ СО РАСХН)
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Application filed by Государственное научное учреждение Институт экспериментальной ветеринарии Сибири и Дальнего ВостокаСибирского отделени Россельхозакадемии (ГНУ ИЭВСиДВ СО РАСХН) (RU), Государственное научное учреждение Институт экспериментальной ветеринарии Сибири и Дальнего Востока Сибирского отделения Россельхозакадемии (ГНУ ИЭВСиДВ СО РАСХН) filed Critical Государственное научное учреждение Институт экспериментальной ветеринарии Сибири и Дальнего ВостокаСибирского отделени Россельхозакадемии (ГНУ ИЭВСиДВ СО РАСХН) (RU)
Priority to RU2004139259/13A priority Critical patent/RU2294742C2/en
Publication of RU2004139259A publication Critical patent/RU2004139259A/en
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Publication of RU2294742C2 publication Critical patent/RU2294742C2/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (3)

1. Способ подавления репродукции вируса вирусной диареи - болезни слизистых крупного рогатого скота в культуре клеток при помощи противовирусного препарата рибавирин, отличающийся тем, что в культуру клеток вносят вирус в дозе 1-10ТЦД50/кл, инкубируют клетки с вирусом в течение 1,5-2 ч, затем вносят противовирусный препарат рибавирин в нетоксичной дозе 50±1 мкг/мл.1. A method of suppressing the reproduction of the virus of viral diarrhea - a disease of the mucous membrane of cattle in a cell culture using the antiviral drug ribavirin, characterized in that the virus is introduced into the cell culture at a dose of 1-10 TTsD 50 / cell, cells with the virus are incubated for 1.5 -2 h, then the antiviral drug ribavirin is introduced in a non-toxic dose of 50 ± 1 μg / ml. 2. Способ по п.1, отличающийся тем, что смесь инкубируют в течение 48 ч при температуре 37±0,2°С в условиях атмосферы 5±0,2% CO2, по окончании срока инкубации вируссодержащую суспензию титруют для определения противовирусного эффекта препарата.2. The method according to claim 1, characterized in that the mixture is incubated for 48 hours at a temperature of 37 ± 0.2 ° C in an atmosphere of 5 ± 0.2% CO 2 , at the end of the incubation period, the virus-containing suspension is titrated to determine the antiviral effect the drug. 3. Способ по п.1, отличающийся тем, что исследования проводят в перевиваемой линии культуры клеток КСТ (коронарные сосуды плода коровы), выращенной микрометодом в 96-луночных культуральных планшетах.3. The method according to claim 1, characterized in that the studies are carried out in a transplantable cell culture line of CTF (coronary vessels of the fetal cow) grown by micromethod in 96-well culture plates.
RU2004139259/13A 2004-12-28 2004-12-28 Method for suppressing the reproduction of viral diarrhea virus - the mucosal disease of cattle in cell culture RU2294742C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2004139259/13A RU2294742C2 (en) 2004-12-28 2004-12-28 Method for suppressing the reproduction of viral diarrhea virus - the mucosal disease of cattle in cell culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2004139259/13A RU2294742C2 (en) 2004-12-28 2004-12-28 Method for suppressing the reproduction of viral diarrhea virus - the mucosal disease of cattle in cell culture

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RU2004139259A true RU2004139259A (en) 2006-06-10
RU2294742C2 RU2294742C2 (en) 2007-03-10

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RU2294742C2 (en) 2007-03-10

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MM4A The patent is invalid due to non-payment of fees

Effective date: 20061229

NF4A Reinstatement of patent

Effective date: 20081027

MM4A The patent is invalid due to non-payment of fees

Effective date: 20101229