RU2004120871A - METHOD FOR PRODUCING TRANSFORMED PLANTS OF GRAIN CROPS - Google Patents

METHOD FOR PRODUCING TRANSFORMED PLANTS OF GRAIN CROPS Download PDF

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Publication number
RU2004120871A
RU2004120871A RU2004120871/13A RU2004120871A RU2004120871A RU 2004120871 A RU2004120871 A RU 2004120871A RU 2004120871/13 A RU2004120871/13 A RU 2004120871/13A RU 2004120871 A RU2004120871 A RU 2004120871A RU 2004120871 A RU2004120871 A RU 2004120871A
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RU
Russia
Prior art keywords
transformed plants
grain crops
producing transformed
kanamycin
plants
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RU2004120871/13A
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Russian (ru)
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RU2279211C2 (en
Inventor
Светлана Алексеевна Данилова (RU)
Светлана Алексеевна Данилова
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Светлана Алексеевна Данилова (RU)
Светлана Алексеевна Данилова
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Priority to RU2004120871/13A priority Critical patent/RU2279211C2/en
Publication of RU2004120871A publication Critical patent/RU2004120871A/en
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Publication of RU2279211C2 publication Critical patent/RU2279211C2/en

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Claims (1)

Способ получения трансформированных растений зерновых культур, заключающийся в обработке эмбриогенного каллуса, незрелых или зрелых зародышей вакуумной инфильтрацией при -1,0÷-0,6 атм в течение 15-60 мин в суспензии Agrobacterium tumefaciens и последующей культивации эксплантов на газоне упомянутых бактерий, предварительно активизированных экссудатом табака, добавляя сначала цефотаксим в концентрации 100-300 мг/л, а затем канамицин для отбора канамицинустойчивых растений.A method of obtaining transformed cereal plants, which consists in processing embryogenic callus, immature or mature embryos by vacuum infiltration at -1.0 ÷ -0.6 atm for 15-60 minutes in a suspension of Agrobacterium tumefaciens and subsequent cultivation of explants on the lawn of the mentioned bacteria, previously activated by tobacco exudate, first adding cefotaxime at a concentration of 100-300 mg / l, and then kanamycin for selection of kanamycin-resistant plants.
RU2004120871/13A 2004-07-09 2004-07-09 Method for obtaining of transformed cereal crop plants RU2279211C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2004120871/13A RU2279211C2 (en) 2004-07-09 2004-07-09 Method for obtaining of transformed cereal crop plants

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Application Number Priority Date Filing Date Title
RU2004120871/13A RU2279211C2 (en) 2004-07-09 2004-07-09 Method for obtaining of transformed cereal crop plants

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RU2004120871A true RU2004120871A (en) 2006-01-10
RU2279211C2 RU2279211C2 (en) 2006-07-10

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RU2004120871/13A RU2279211C2 (en) 2004-07-09 2004-07-09 Method for obtaining of transformed cereal crop plants

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1979483A4 (en) * 2006-01-11 2010-09-01 Molecular Plant Breeding Nominees Ltd Method of producing transgenic graminaceous cells and plants

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Effective date: 20070710