RU2003133644A - METHOD FOR PRODUCING DOUBLE-FIBER RIBONUCLEIC ACID - Google Patents

METHOD FOR PRODUCING DOUBLE-FIBER RIBONUCLEIC ACID Download PDF

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Publication number
RU2003133644A
RU2003133644A RU2003133644/13A RU2003133644A RU2003133644A RU 2003133644 A RU2003133644 A RU 2003133644A RU 2003133644/13 A RU2003133644/13 A RU 2003133644/13A RU 2003133644 A RU2003133644 A RU 2003133644A RU 2003133644 A RU2003133644 A RU 2003133644A
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RU
Russia
Prior art keywords
chloroform
ribonucleic acid
acid
fiber
producing double
Prior art date
Application number
RU2003133644/13A
Other languages
Russian (ru)
Other versions
RU2302464C2 (en
Inventor
Наталь Владимировна Бажутина (RU)
Наталья Владимировна Бажутина
Николай Борисович Бажутин (RU)
Николай Борисович Бажутин
Наталь Васильевна Белова (RU)
Наталья Васильевна Белова
Тать на Леонидовна Гурьева (RU)
Татьяна Леонидовна Гурьева
Владимир Павлович Гурьев (RU)
Владимир Павлович Гурьев
Светлана Альбертовна Дегтева (RU)
Светлана Альбертовна Дегтева
Раиса Вильевна Еникеева (RU)
Раиса Вильевна Еникеева
Владимир Викторович Золин (RU)
Владимир Викторович Золин
Тать на Леонидовна Комарова (RU)
Татьяна Леонидовна Комарова
Алексей Александрович Колокольцов (RU)
Алексей Александрович Колокольцов
Ольга Афанасьевна Моисеенкова (RU)
Ольга Афанасьевна Моисеенкова
Нина Васильевна Наумова (RU)
Нина Васильевна Наумова
Елена Витальевна Остапенко (RU)
Елена Витальевна Остапенко
Марина Ивановна Пикалова (RU)
Марина Ивановна Пикалова
жко Земфира Марсовна Самот (RU)
Земфира Марсовна Самотяжко
Сергей Николаевич Таргонский (RU)
Сергей Николаевич Таргонский
Ольга Викторовна Шарафаненко (RU)
Ольга Викторовна Шарафаненко
Лариса Викторовна Шамрина (RU)
Лариса Викторовна Шамрина
Original Assignee
Николай Борисович Бажутин (RU)
Николай Борисович Бажутин
Сергей Николаевич Таргонский (RU)
Сергей Николаевич Таргонский
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Николай Борисович Бажутин (RU), Николай Борисович Бажутин, Сергей Николаевич Таргонский (RU), Сергей Николаевич Таргонский filed Critical Николай Борисович Бажутин (RU)
Priority to RU2003133644/13A priority Critical patent/RU2302464C2/en
Publication of RU2003133644A publication Critical patent/RU2003133644A/en
Application granted granted Critical
Publication of RU2302464C2 publication Critical patent/RU2302464C2/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (1)

Способ получения двуспиральной рибонуклеиновой кислоты, включающий дезинтеграцию биомассы дрожжей в буферном растворе, содержащем культуральную жидкость Arthrobacter luteus ATSS 21606, депротеинизацию гомогената термообработкой, выделение двуспиральной РНК фракционированием нуклеиновых кислот хлористым литием, очистку двуспиральной рибонуклеиновой кислоты хлороформом или смесью хлороформа с изоамиловым спиртом и ее осаждение этанолом, отличающийся тем, что дезинтеграцию биомассы дрожжей осуществляют автолизом путем ее инкубирования при температуре 30-40°С в течение 15-20 ч, экстракт перед фракционированием нуклеиновых кислот очищают и концентрируют путем его ультрафильтрации на мембранах с пределом отсечения не менее 100 КДа.A method for producing double-stranded ribonucleic acid, including disintegration of yeast biomass in a buffer solution containing Arthrobacter luteus ATSS 21606 culture fluid, deproteinization of the homogenate by heat treatment, isolation of double-stranded RNA by lithium chloride fractionation with chloroform or chloroform of chloroform and chloroform acid-chloroform and chloroform acid chloroform , characterized in that the disintegration of the biomass of yeast is carried out by autolysis by incubation while erature 30-40 ° C for 15-20 hours, the extract before fractionation of nucleic acids purified and concentrated it by ultrafiltration on membranes with cut-off limit of no less than 100 KDa.
RU2003133644/13A 2003-11-12 2003-11-12 Method for production of double-helical ribonucleic acid RU2302464C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2003133644/13A RU2302464C2 (en) 2003-11-12 2003-11-12 Method for production of double-helical ribonucleic acid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2003133644/13A RU2302464C2 (en) 2003-11-12 2003-11-12 Method for production of double-helical ribonucleic acid

Publications (2)

Publication Number Publication Date
RU2003133644A true RU2003133644A (en) 2005-05-10
RU2302464C2 RU2302464C2 (en) 2007-07-10

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ID=35746392

Family Applications (1)

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RU2003133644/13A RU2302464C2 (en) 2003-11-12 2003-11-12 Method for production of double-helical ribonucleic acid

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RU (1) RU2302464C2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2558256C1 (en) * 2014-05-13 2015-07-27 Федеральное бюджетное учреждение науки "Государственный научный центр вирусологии и биотехнологии "Вектор" (ФБУН ГНЦ ВБ "Вектор") METHOD OF PRODUCTION OF DOUBLE-STRANDED RIBONUCLEIC ACID (dsRNA) FROM CELLS OF YEAST Saccharomyces cerevisiae

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Publication number Publication date
RU2302464C2 (en) 2007-07-10

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FA92 Acknowledgement of application withdrawn (lack of supplementary materials submitted)

Effective date: 20060116

FZ9A Application not withdrawn (correction of the notice of withdrawal)

Effective date: 20061025

PC4A Invention patent assignment

Effective date: 20091026

MM4A The patent is invalid due to non-payment of fees

Effective date: 20181113