RU2002132957A - ANTIBODIES AND SCFV IMMUNOTOXINS SPECIFIC TO RICHTER'S IMPLIED ANTS AND THEIR APPLICATION - Google Patents

ANTIBODIES AND SCFV IMMUNOTOXINS SPECIFIC TO RICHTER'S IMPLIED ANTS AND THEIR APPLICATION Download PDF

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Publication number
RU2002132957A
RU2002132957A RU2002132957/13A RU2002132957A RU2002132957A RU 2002132957 A RU2002132957 A RU 2002132957A RU 2002132957/13 A RU2002132957/13 A RU 2002132957/13A RU 2002132957 A RU2002132957 A RU 2002132957A RU 2002132957 A RU2002132957 A RU 2002132957A
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RU
Russia
Prior art keywords
richter
ants
cells
antibodies
uterus
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Application number
RU2002132957/13A
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Russian (ru)
Inventor
Роберт САНДЕРС (US)
Роберт САНДЕРС
Кимберли КЛАЙН (US)
Кимберли КЛАЙН
Original Assignee
Рисерч Дивелопмент Фаундейшн (US)
Рисерч Дивелопмент Фаундейшн
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Application filed by Рисерч Дивелопмент Фаундейшн (US), Рисерч Дивелопмент Фаундейшн filed Critical Рисерч Дивелопмент Фаундейшн (US)
Publication of RU2002132957A publication Critical patent/RU2002132957A/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (4)

1. Способ получения моноклонального антитела и фрагментов Fab co специфичностью к антигенам микроворсинок средней кишки матки завезенных муравьев Рихтера, включающий:1. A method of obtaining a monoclonal antibody and Fab fragments with specificity for antigens of the microvilli of the midgut of the uterus of imported Richter ants, including: иммунизацию животного указанными целевыми клетками для получения моноклональных антител;immunizing an animal with said target cells to produce monoclonal antibodies; сбор обогащенных клеток селезенки;collection of enriched spleen cells; гибридизацию указанных клеток селезенки со сливающимся клетками миеломы, используя полиэтиленгликоль;hybridization of these spleen cells with fused myeloma cells using polyethylene glycol; отбор клеток гибридомы выращиванием в среде НАТ;selection of hybridoma cells by growing in NAT medium; скрининг надосадочных жидкостей гибридомы для получения мышиных антител, используя методику ELISA и для получения антител специфичных к антигенам микроворсинок средней кишки матки завезенных муравьев Рихтера, но не к антигенам естественных муравьев Рихтера, используя иммуногистологические методики;hybridoma supernatant screening to obtain murine antibodies using the ELISA technique and to obtain antibodies specific to the antigens of the intestinal microvilli of the uterus of the uterus of the imported Richter ants, but not to the antigens of Richter's natural ants using immunohistological techniques; клонирование ограничивающим разведением, скрининг надсадочных жидкостей гибридомы, экспансию клонов и замораживание положительных клонов; иlimiting dilution cloning, hybridoma supernatant screening, expansion of clones, and freezing of positive clones; and очистку моноклонального антитела и Fab фрагментов со специфичностью к антигенам микроворсинок средней кишки матки завезенных муравьев Рихтера.purification of monoclonal antibodies and Fab fragments with specificity for antigens of the microvilli of the midgut of the uterus of imported Richter ants. 2. Способ по п.1, отличающийся тем, что указанным животным является мышь.2. The method according to claim 1, characterized in that said animal is a mouse. 3. Способ по п.1, отличающийся тем, что указанными целевыми клетками являются клетки микроворсинок средней кишки завезенных муравьев Рихтера.3. The method according to claim 1, characterized in that said target cells are cells of the microvilli of the middle intestine of the imported Richter ants. 4. Моноклональное антитело или Fab фрагмент, полученное способом по п.1.4. Monoclonal antibody or Fab fragment obtained by the method according to claim 1.
RU2002132957/13A 1997-07-10 2002-12-06 ANTIBODIES AND SCFV IMMUNOTOXINS SPECIFIC TO RICHTER'S IMPLIED ANTS AND THEIR APPLICATION RU2002132957A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5213297P 1997-07-10 1997-07-10
US60/052,132 1997-07-10

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
RU2000103044/13A Division RU2208641C2 (en) 1997-07-10 1998-07-09 Method for preparing polypeptide specific to imported fire ant, polypeptide, method for fire ant killing

Publications (1)

Publication Number Publication Date
RU2002132957A true RU2002132957A (en) 2005-01-27

Family

ID=21975684

Family Applications (2)

Application Number Title Priority Date Filing Date
RU2000103044/13A RU2208641C2 (en) 1997-07-10 1998-07-09 Method for preparing polypeptide specific to imported fire ant, polypeptide, method for fire ant killing
RU2002132957/13A RU2002132957A (en) 1997-07-10 2002-12-06 ANTIBODIES AND SCFV IMMUNOTOXINS SPECIFIC TO RICHTER'S IMPLIED ANTS AND THEIR APPLICATION

Family Applications Before (1)

Application Number Title Priority Date Filing Date
RU2000103044/13A RU2208641C2 (en) 1997-07-10 1998-07-09 Method for preparing polypeptide specific to imported fire ant, polypeptide, method for fire ant killing

Country Status (12)

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EP (1) EP1000357A4 (en)
JP (1) JP2001509397A (en)
KR (1) KR20010021624A (en)
CN (1) CN1148577C (en)
AU (1) AU739004B2 (en)
CA (1) CA2295914A1 (en)
IL (1) IL133666A0 (en)
NZ (1) NZ501963A (en)
RU (2) RU2208641C2 (en)
TW (1) TW585921B (en)
WO (1) WO1999002991A1 (en)
ZA (1) ZA986114B (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2856075B1 (en) * 2003-06-16 2007-10-12 Monoclonal Antibodies Therapeu ESSENTIALLY AUTOMATED LARGE-SCALE SCREENING METHOD OF CELLS SECURING MONOCLONAL ANTIBODIES
GB0422431D0 (en) * 2004-10-08 2004-11-10 Affitech As Method
ATE507245T1 (en) * 2005-03-11 2011-05-15 Syngenta Ltd CONTROL OF HARMFUL RODENTS
CN100402666C (en) * 2005-10-21 2008-07-16 中山大学 Method of identifying invasion of south American glim ant and its nucleic acid sequence, probe and reagent kit
US20130224226A1 (en) * 2010-08-26 2013-08-29 Agrosavfe N.V. Insect binding antibodies
GB201108598D0 (en) 2011-05-20 2011-07-06 Shotter Nicholas R Front suspension system
EP3415010A1 (en) 2017-06-13 2018-12-19 Agrosavfe Nv Insect-controlling polypeptides and methods
RU2731516C1 (en) * 2019-08-01 2020-09-03 Федеральное государственное унитарное предприятие «Государственный научно-исследовательский институт особо чистых биопрепаратов» Федерального медико-биологического агентства Anaphylatoxin c5a antibodies to human

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0852011B1 (en) * 1995-09-14 2004-03-31 The Regents of the University of California ANTIBODIES SPECIFIC FOR NATIVE PrP?Sc

Also Published As

Publication number Publication date
WO1999002991A1 (en) 1999-01-21
JP2001509397A (en) 2001-07-24
ZA986114B (en) 2000-01-10
EP1000357A1 (en) 2000-05-17
CN1148577C (en) 2004-05-05
KR20010021624A (en) 2001-03-15
AU739004B2 (en) 2001-10-04
AU8389698A (en) 1999-02-08
CA2295914A1 (en) 1999-01-21
RU2208641C2 (en) 2003-07-20
CN1263598A (en) 2000-08-16
EP1000357A4 (en) 2005-05-25
NZ501963A (en) 2001-08-31
IL133666A0 (en) 2001-04-30
TW585921B (en) 2004-05-01

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Effective date: 20060110