RU2002115475A - Enzyme-linked immunosorbent assay for determining the soluble form of HLA-DR antigens in human serum - Google Patents

Enzyme-linked immunosorbent assay for determining the soluble form of HLA-DR antigens in human serum

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Publication number
RU2002115475A
RU2002115475A RU2002115475/15A RU2002115475A RU2002115475A RU 2002115475 A RU2002115475 A RU 2002115475A RU 2002115475/15 A RU2002115475/15 A RU 2002115475/15A RU 2002115475 A RU2002115475 A RU 2002115475A RU 2002115475 A RU2002115475 A RU 2002115475A
Authority
RU
Russia
Prior art keywords
hla
antigens
determining
soluble form
enzyme
Prior art date
Application number
RU2002115475/15A
Other languages
Russian (ru)
Other versions
RU2237249C2 (en
Inventor
кова Наталь Евгеньевна Худ
Наталья Евгеньевна Худякова
Тамара Геннадьевна Мартынова
Юли Сергеевна Птицына
Юлия Сергеевна Птицына
Виктор Владимирович Новиков
Original Assignee
Федеральное государственное учреждениие науки и здравоохранени "Нижегородский научно-исследовательский иинститут эпидемиологии и микробиологии им. акад. И.Н. Блохиной"
Федеральное государственное учреждениие науки и здравоохранения "Нижегородский научно-исследовательский иинститут эпидемиологии и микробиологии им. акад. И.Н. Блохиной"
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Application filed by Федеральное государственное учреждениие науки и здравоохранени "Нижегородский научно-исследовательский иинститут эпидемиологии и микробиологии им. акад. И.Н. Блохиной", Федеральное государственное учреждениие науки и здравоохранения "Нижегородский научно-исследовательский иинститут эпидемиологии и микробиологии им. акад. И.Н. Блохиной" filed Critical Федеральное государственное учреждениие науки и здравоохранени "Нижегородский научно-исследовательский иинститут эпидемиологии и микробиологии им. акад. И.Н. Блохиной"
Priority to RU2002115475/15A priority Critical patent/RU2237249C2/en
Publication of RU2002115475A publication Critical patent/RU2002115475A/en
Application granted granted Critical
Publication of RU2237249C2 publication Critical patent/RU2237249C2/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Claims (1)

Способ определения растворимой формы HLA-DR антигенов в сыворотке крови человека, включающий иммобилизацию специфических антител на твердом носителе, последовательную инкубацию с исследуемой сывороткой и пероксидазным конъюгатом, и количественным выявлением окрашенного продукта пероксидазной активности, отличающийся тем, что, с целью упрощения и удешевления способа с сохранением высокой чувствительности и специфичности, в качестве первых антител используют поликлональные антитела против поверхностных антигенов мононуклеарных клеток человека, а в качестве вторых антител применяют HLA-DR-специфичные моноклональные антитела МКА ИКО-1, конъюгированные с пероксидазой хрена.A method for determining the soluble form of HLA-DR antigens in human blood serum, including the immobilization of specific antibodies on a solid carrier, sequential incubation with the test serum and peroxidase conjugate, and the quantification of a colored product of peroxidase activity, characterized in that, in order to simplify and reduce the cost of the method with maintaining high sensitivity and specificity, polyclonal antibodies against surface antigens of mononuclear cells are used as the first antibodies to man, and as the second antibody used HLA-DR-specific monoclonal antibody mAb PPI-1 conjugated to horseradish peroxidase.
RU2002115475/15A 2002-06-10 2002-06-10 Enzyme immunoassay method for determination of soluble form of antigens hla-dr in human blood serum RU2237249C2 (en)

Priority Applications (1)

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RU2002115475/15A RU2237249C2 (en) 2002-06-10 2002-06-10 Enzyme immunoassay method for determination of soluble form of antigens hla-dr in human blood serum

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Application Number Priority Date Filing Date Title
RU2002115475/15A RU2237249C2 (en) 2002-06-10 2002-06-10 Enzyme immunoassay method for determination of soluble form of antigens hla-dr in human blood serum

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RU2002115475A true RU2002115475A (en) 2004-01-10
RU2237249C2 RU2237249C2 (en) 2004-09-27

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Effective date: 20060611