RU2001123408A - A set of oligonucleotides primers for the identification of RNA of the Crimean Congo virus hemorrhagic fever - Google Patents

A set of oligonucleotides primers for the identification of RNA of the Crimean Congo virus hemorrhagic fever

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Publication number
RU2001123408A
RU2001123408A RU2001123408/13A RU2001123408A RU2001123408A RU 2001123408 A RU2001123408 A RU 2001123408A RU 2001123408/13 A RU2001123408/13 A RU 2001123408/13A RU 2001123408 A RU2001123408 A RU 2001123408A RU 2001123408 A RU2001123408 A RU 2001123408A
Authority
RU
Russia
Prior art keywords
identification
hemorrhagic fever
rna
virus hemorrhagic
crimean congo
Prior art date
Application number
RU2001123408/13A
Other languages
Russian (ru)
Other versions
RU2209830C2 (en
Inventor
Владимир Семенович Петров
Ирина Дмитриевна Петрова
Георгий Иванович Тюнников
Сергей Викторович Серегин
Людмила Николаевна Яшина
Ирина Ивановна Кузина
Original Assignee
Государственный научный центр вирусологии и биотехнологии "Вектор"
Filing date
Publication date
Application filed by Государственный научный центр вирусологии и биотехнологии "Вектор" filed Critical Государственный научный центр вирусологии и биотехнологии "Вектор"
Priority to RU2001123408A priority Critical patent/RU2209830C2/en
Priority claimed from RU2001123408A external-priority patent/RU2209830C2/en
Publication of RU2001123408A publication Critical patent/RU2001123408A/en
Application granted granted Critical
Publication of RU2209830C2 publication Critical patent/RU2209830C2/en

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Claims (1)

Набор олигонуклеотдов-праймеров для идентификации вируса Крымской-Конго геморрагической лихорадки, содержащий 2 пары дезоксиолигонуклеотидов, обладающих активностью прямого и обратного праймеров в реакции обратной транскрипции и полимеразной цепной реакции со следующей структурой:A set of primer oligonucleotides for the identification of the Crimean Congo virus hemorrhagic fever, containing 2 pairs of deoxyoligonucleotides with direct and reverse primer activity in the reverse transcription reaction and polymerase chain reaction with the following structure: 5’agagactgaggggaagggag 3’ - PS1; 5’aattgtcttggcacacggat 3’ - PS2; 5’caaaaagactgtagaggctc 3’ - PS3; 5’ttgtccgtaggttaagaatg 3’ - PS4,5’agagactgaggggaagggag 3 ’- PS1; 5’aattgtcttggcacacggat 3 ’- PS2; 5’caaaaagactgtagaggctc 3 ’- PS3; 5’ttgtccgtaggttaagaatg 3 ’- PS4, а также их аналоги с вырожденной структурой:as well as their analogues with a degenerate structure: Ps1* 5’-agagac(t,c)gaggggaa(a,g)ggag-3’Ps1 * 5’-agagac (t, c) gaggggaa (a, g) ggag-3 ’ Ps2* 5’-aattgtcttggc(a,g)ca(t,c)ggat-3’Ps2 * 5’-aattgtcttggc (a, g) ca (t, c) ggat-3 ’ Ps3* 5’-caa(a,g)aagac(t,c)gt(a,g)gaggctc-3’Ps3 * 5’-caa (a, g) aagac (t, c) gt (a, g) gaggctc-3 ’ Ps4* 5’-ttgtccg(t,a)aggtt(a,g)ag(a,g)atg-3’Ps4 * 5’-ttgtccg (t, a) aggtt (a, g) ag (a, g) atg-3 ’ и прямой контрольный праймер со следующей структурой:and a direct control primer with the following structure: Ps5 5’-ct(t,c)tcagctc(a,g)t(t,a)ctactggct-3’.Ps5 5’-ct (t, c) tcagctc (a, g) t (t, a) ctactggct-3 ’.
RU2001123408A 2001-08-20 2001-08-20 Set of oligonucleotides-primers for identification of hemorrhagic fever crimean-congo virus rna RU2209830C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2001123408A RU2209830C2 (en) 2001-08-20 2001-08-20 Set of oligonucleotides-primers for identification of hemorrhagic fever crimean-congo virus rna

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2001123408A RU2209830C2 (en) 2001-08-20 2001-08-20 Set of oligonucleotides-primers for identification of hemorrhagic fever crimean-congo virus rna

Publications (2)

Publication Number Publication Date
RU2001123408A true RU2001123408A (en) 2003-06-10
RU2209830C2 RU2209830C2 (en) 2003-08-10

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU2001123408A RU2209830C2 (en) 2001-08-20 2001-08-20 Set of oligonucleotides-primers for identification of hemorrhagic fever crimean-congo virus rna

Country Status (1)

Country Link
RU (1) RU2209830C2 (en)

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