RU2001100147A - COMBINANT PLASMIDA DNA PTBI-HBSAG CONTAINING TBI-HBSAG CHIMERIC GENE UNDER CONTROL OF THE P7.5 K ASPIRATOUS VACCIN VIRUS AND STRAIN OF RECOMBINANT INFLAMMATION OF AN EVOLVIT - Google Patents

Claims (2)

1. Recombinant plasmid DNA p18 encoding the chimeric TBI-HBsAg gene with a molecular weight of 4.2 Mda and a size of 6322 bp containing ClaI-BamHI vector fragment of plasmid p7.5S113 DNA size 4647 p. N. with the nucleotide sequence of the vaccinia virus thymidine kinase gene with the vaccinia virus P7.5 promoter integrated therein; BglII-PstI synthetic oligonucleotide 8 bp ensuring the correct docking of the TBI gene with a translation initiation site located in the sequence of a vector plasmid having the structure 5'-GATCTGCA-3 ', PstI-Kpn2I DNA fragment 432 bp encoding an artificial TBI polypeptide immunogenic against HIV-1, Kpn2I-BglII synthetic 17 bp DNA fragment connecting the genes of the TBI and S proteins in the correct translation frame, having the structure 5'-CCGGAATTCTCGAGGTA-3 '3'-TTAAGAGCTCCATCTAG-5'; BamHI-ClaI fragment of the hepatitis B virus genome encoding surface virion protein S (HBsAg), size 1226 bp ; genetic markers: bla - gene ampitsillinrezistentnosti - β-lactamase gene, the ampicillin resistance determining when transformed cells E. coli, TK - interrupted thymidine kinase gene of vaccinia virus that produces the TK ^- phenotype insertions at the recombination into the genome of TK ⁺ vaccinia virus, unique restriction sites: HindIII - position 1, KpnI-1303, Kpn2I-1453, XhoI-1462.  2. The strain of the recombinant vaccinia virus VR18 V-312 - producer of the chimeric protein TBI-HBsAg.