RU2000132968A - WAYS OF ACCELERATED DIAGNOSTICS OF HEPATITIS C, BASED ON DETECTION OF HEPATITIS C VIRUS HEMAGLUTININS (HCV) AND ANTIBODIES TO THEM IN RHA AND RGT IN PEOPLE BLOOD - Google Patents

WAYS OF ACCELERATED DIAGNOSTICS OF HEPATITIS C, BASED ON DETECTION OF HEPATITIS C VIRUS HEMAGLUTININS (HCV) AND ANTIBODIES TO THEM IN RHA AND RGT IN PEOPLE BLOOD

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Publication number
RU2000132968A
RU2000132968A RU2000132968/14A RU2000132968A RU2000132968A RU 2000132968 A RU2000132968 A RU 2000132968A RU 2000132968/14 A RU2000132968/14 A RU 2000132968/14A RU 2000132968 A RU2000132968 A RU 2000132968A RU 2000132968 A RU2000132968 A RU 2000132968A
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RU
Russia
Prior art keywords
hepatitis
hcv
virus
detection
antibodies
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RU2000132968/14A
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Russian (ru)
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RU2194993C2 (en
Inventor
Петр Григорьевич Дерябин
Елена Ивановна Исаева
Дмитрий Константинович Львов
Original Assignee
Научно-исследовательский институт вирусологии им. Д.И. Ивановского
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Priority to RU2000132968A priority Critical patent/RU2194993C2/en
Priority claimed from RU2000132968A external-priority patent/RU2194993C2/en
Publication of RU2000132968A publication Critical patent/RU2000132968A/en
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Publication of RU2194993C2 publication Critical patent/RU2194993C2/en

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Claims (6)

1. Способ диагностики гепатита С, предусматривающий использование (исследование) крови людей, зараженных или подозрительных на заражение вирусом гепатита С, отличающийся тем, что обнаружения (выявления) антигенов вируса гепатита С (ВГС), основано на способности антигенов ВГС агглютинировать эритроциты (РГА - реакция гемагглютинации).1. A method for the diagnosis of hepatitis C, involving the use (study) of the blood of people infected or suspected of being infected with the hepatitis C virus, characterized in that the detection (detection) of hepatitis C virus antigens (HCV) is based on the ability of the HCV antigens to agglutinate red blood cells (RGA - hemagglutination reaction). 2. Способ по п. 1, отличающийся тем, что для реакции гемагглютинации используют эритроциты гусей. 2. The method according to p. 1, characterized in that for the hemagglutination reaction using red blood cells of geese. 3. Способ по п. 1, отличающийся тем, что для реакции гемагглютинации используют эритроциты голубей. 3. The method according to p. 1, characterized in that for the hemagglutination reaction using red blood cells of pigeons. 4. Способ по любому из предшествующих пунктов, отличающийся тем, что реакцию гемагглютинации ставят в зоне рН 6,1 - 6,5. 4. The method according to any one of the preceding paragraphs, characterized in that the hemagglutination reaction is placed in a pH range of 6.1 to 6.5. 5. Способ по 4, отличающийся тем, что в качестве буферной смеси в РГА используют боратный буфер рН 9,0, содержащий 0,4% бычьего альбумина и смесь фосфатных буферов рН 6.0 и рН 7.0. 5. The method according to 4, characterized in that the borate buffer pH 9.0 containing 0.4% bovine albumin and a mixture of phosphate buffers pH 6.0 and pH 7.0 are used as a buffer mixture in the RGA. 6. Способ иммунодиагностики ВГС инфекции, предусматривающий выявление антител к ВГС в сыворотке крови людей инфицированных вирусом гепатита С, отличающийся тем, что с помощью антигенов цитопатогенных вариантов ВГС, выделенных из инфицированных ВГС культур клеток и/или органов зараженных ВГС животных определяют антигемагглютинины ВГС в РТГА - в реакции торможения гемаглютинации. 6. A method for the immunodiagnosis of HCV infection, which involves the detection of antibodies to HCV in the blood serum of people infected with hepatitis C virus, characterized in that antigens of HCV cultures of cells and / or organs of animals infected with HCV are determined by HCV antigens of HCV in the RTHA - in the reaction of inhibition of hemagglutination.
RU2000132968A 2000-12-28 2000-12-28 Method for setting accelerated diagnosis of hepatitis c based on hepatitis c virus hemagglutinin and their antibodies detection in hemagglutination reaction and in hemagglutination inhibition reaction RU2194993C2 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2542475C2 (en) * 2013-06-18 2015-02-20 Федеральное казенное учреждение здравоохранения "Ростовский-на-Дону ордена Трудового Красного Знамени научно-исследовательский противочумный институт" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека Method for preparing diagnosticum for detecting hepatitis c antigen in reaction of volumetric agglomeration (rva)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120196272A1 (en) * 2010-08-05 2012-08-02 Roche Molecular Systems, Inc. Prediction of HCV Viral Kinetics in Interferon-Free Treatment

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2542475C2 (en) * 2013-06-18 2015-02-20 Федеральное казенное учреждение здравоохранения "Ростовский-на-Дону ордена Трудового Красного Знамени научно-исследовательский противочумный институт" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека Method for preparing diagnosticum for detecting hepatitis c antigen in reaction of volumetric agglomeration (rva)

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