RU2000122615A - TRANSFORMATION OF VEGETRIAN CELL UNITS AND VEGETABLE TISSUES-MEDIATED MEDIA AND REGENERATION OF PLANTS OF THEM - Google Patents

TRANSFORMATION OF VEGETRIAN CELL UNITS AND VEGETABLE TISSUES-MEDIATED MEDIA AND REGENERATION OF PLANTS OF THEM

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Publication number
RU2000122615A
RU2000122615A RU2000122615/13A RU2000122615A RU2000122615A RU 2000122615 A RU2000122615 A RU 2000122615A RU 2000122615/13 A RU2000122615/13 A RU 2000122615/13A RU 2000122615 A RU2000122615 A RU 2000122615A RU 2000122615 A RU2000122615 A RU 2000122615A
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Russia
Prior art keywords
cell
type
plant
transformation
plant cell
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Application number
RU2000122615/13A
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Russian (ru)
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RU2225444C2 (en
Inventor
Джозеф Ф. Петолино
Даякар Р. ПАРЕДДИ
Николь Л. ХОПКИНЗ
Кэтрин АРМСТРОНГ
Original Assignee
Дау Агросайенсиз Ллс
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Publication of RU2000122615A publication Critical patent/RU2000122615A/en
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Publication of RU2225444C2 publication Critical patent/RU2225444C2/en

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Claims (11)

1. Способ трансформации растительной клетки, включающий (a) отбор по крайней мере одной клетки каллюсной культуры I типа, II типа, производной от гипокотиля или производной от семядоли, или клеточной ткани меристемы, пыльцы, семядоли или половой клетки; и (b) внесение ДНК в упомянутую клетку с применением "вискер-опосредованной" трансформации.1. A method for transforming a plant cell, comprising (a) selecting at least one cell of a callus culture of type I, type II, a derivative of a hypocotyl or a derivative of a cotyledon, or cell tissue of a meristem, pollen, cotyledon or germ cell; and (b) introducing DNA into said cell using a "whisker-mediated" transformation. 2. Способ получения фертильного трансгенного растения, который включает регенерирование клетки, полученной в способе по п. 1. 2. A method of obtaining a fertile transgenic plant, which includes regenerating the cell obtained in the method according to claim 1. 3. Способ получения фертильного трансгенного растения, который включает следующие этапы: (i) постановку регенерируемой каллюсной культуры на материале растения, которое предполагается трансформировать, при том, что упомянутая каллюсная культура выбирается из группы, включающей каллюсные культуры I типа, II типа, производные от гипокотиля или производные от семядоли; (ii) отбор агрегатов растительных клеток, предназначенных для трансформации; (iii) трансформацию упомянутого агрегата растительных клеток с использованием ДНК путем "вискер-опосредованной" трансформации; (iv) идентификацию трансформированных клеточных линий ; и (v) регенерацию из них фертильных трансгенных растений. 3. A method of obtaining a fertile transgenic plant, which includes the following steps: (i) placing the regenerated callus culture on the plant material to be transformed, while said callus culture is selected from the group comprising callus cultures of type I, type II, derived from hypocotyl or derivatives of cotyledons; (ii) selection of plant cell aggregates intended for transformation; (iii) transforming said plant cell aggregate using DNA by a "whisker-mediated" transformation; (iv) identification of transformed cell lines; and (v) regeneration thereof of fertile transgenic plants. 4. Способ по п. 3, отличающийся тем, что упомянутая каллюсная культура I типа формируется на материале кукурузы Zea mays или риса Oryza sativa. 4. The method according to p. 3, characterized in that the said callus culture of type I is formed on the material of maize Zea mays or rice Oryza sativa. 5. Способ по п. 3, отличающийся тем, что упомянутая каллюсная культура II типа формируется на материале кукурузы Zea mays. 5. The method according to p. 3, characterized in that the said callus culture of type II is formed on the material of maize Zea mays. 6. Способ по п. 3, отличающийся тем, что упомянутая культура, производная от гипокотилей, формируется на материале хлопчатника Gossypium hirsutum. 6. The method according to p. 3, characterized in that the said culture, a derivative of hypocotyls, is formed on the material of cotton Gossypium hirsutum. 7. Способ по п. 3, отличающийся тем, что упомянутая культура, производная от семядолей, формируется на материале хлопчатника Gossypium hirsutum. 7. The method according to p. 3, characterized in that the said crop, derived from cotyledons, is formed on the material of cotton Gossypium hirsutum. 8. Способ по п. 3, отличающийся тем, что упомянутый агрегат растительных клеток инициируется на твердой среде. 8. The method according to p. 3, characterized in that the said plant cell aggregate is initiated on a solid medium. 9. Фертильное трансгенное растение, полученное в соответствии со способом по любому из пп. 3-8. 9. Fertile transgenic plant obtained in accordance with the method according to any one of paragraphs. 3-8. 10. ДНК-конструкция, являющаяся функциональной в растительной клетке, включающая в направлении 5′_→3′ транскрипции участок регуляции транскрипции, функциональный в упомянутой растительной клетке, и последовательность ДНК, показанной в SEQ ID NO 1, и представляющий интерес ген, при том, что упомянутый ген находится либо в смысловой, либо в антисмысловой ориентации. 10. A DNA construct that is functional in a plant cell, including in the direction of 5′_ → 3 ′ transcription, a transcriptional regulation region functional in said plant cell, and the DNA sequence shown in SEQ ID NO 1, and a gene of interest, wherein that the gene mentioned is either in the sense or in the antisense orientation. 11. Сегмент регуляции транскрипции по п. 10, имеющий последовательность ДНК SEQ ID NO 1. 11. The transcriptional regulatory segment of claim 10, having the DNA sequence of SEQ ID NO 1.
RU2000122615/13A 1998-01-29 1999-01-28 "visker"-mediated transformation of aggregates of vegetable cells and vegetable tissues and plants regeneration form them RU2225444C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7294498P 1998-01-29 1998-01-29
US60/072,944 1998-01-29

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RU2000122615A true RU2000122615A (en) 2002-09-20
RU2225444C2 RU2225444C2 (en) 2004-03-10

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US (4) US6350611B1 (en)
EP (1) EP1051496A1 (en)
JP (1) JP2002528047A (en)
KR (1) KR100573656B1 (en)
CN (1) CN1155709C (en)
AR (1) AR014491A1 (en)
AU (1) AU764098B2 (en)
BR (1) BR9907994A (en)
CA (2) CA2318728C (en)
IL (2) IL137519A0 (en)
RU (1) RU2225444C2 (en)
WO (1) WO1999038979A1 (en)

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