RU2000115566A - SPECIFIC FOR BREASTARY TISSUE EXPRESSION SYSTEM USING A BETA-CASINE GENE PROMOTIONAL SITE OF THE KOREAN LOCAL GOAT - Google Patents

SPECIFIC FOR BREASTARY TISSUE EXPRESSION SYSTEM USING A BETA-CASINE GENE PROMOTIONAL SITE OF THE KOREAN LOCAL GOAT

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Publication number
RU2000115566A
RU2000115566A RU2000115566/13A RU2000115566A RU2000115566A RU 2000115566 A RU2000115566 A RU 2000115566A RU 2000115566/13 A RU2000115566/13 A RU 2000115566/13A RU 2000115566 A RU2000115566 A RU 2000115566A RU 2000115566 A RU2000115566 A RU 2000115566A
Authority
RU
Russia
Prior art keywords
pgbc
gene
vector
breastary
casine
Prior art date
Application number
RU2000115566/13A
Other languages
Russian (ru)
Other versions
RU2191826C2 (en
Inventor
Оок Йоон ЙОО
Кенг Кванг ЛИИ
Йонг Ман ХАН
Сун Юнг КИМ
Хае Йонг ДЖЕОНГ
Юнг Хо КО
Вон Джун ОХ
Original Assignee
Ханми Фарм. Ко., ЛТД
Корея Эдвансд Инститьют оф Сайенс энд Текнолоджи
Filing date
Publication date
Application filed by Ханми Фарм. Ко., ЛТД, Корея Эдвансд Инститьют оф Сайенс энд Текнолоджи filed Critical Ханми Фарм. Ко., ЛТД
Priority claimed from PCT/KR1998/000277 external-priority patent/WO2000015808A1/en
Publication of RU2000115566A publication Critical patent/RU2000115566A/en
Application granted granted Critical
Publication of RU2191826C2 publication Critical patent/RU2191826C2/en

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Claims (1)

1. Рекомбинантный вектор pGbc, использующий промоторный сайт гена β-казеина корейских местных козлов.1. Recombinant vector pGbc using the promoter site of the β-casein gene of Korean local goats. 2. Рекомбинантный вектор pGbc_L, использующий промоторный сайт гена β-казеина корейских местных козлов. 2. Recombinant vector pGbc_L using the promoter site of the β-casein gene of the Korean local goats. 3. Рекомбинантный вектор pGbc_S, использующий промоторный сайт гена β-казеина корейских местных козлов. 3. Recombinant vector pGbc_S using the promoter site of the β-casein gene of the Korean local goats. 4. Способ получения физиологически активных веществ, в котором физиологически активные вещества экспрессируются с использованием любого из векторов pGbc, pGbcJL и pGbc_L и pGbc_S по пп. 1-3. 4. A method of producing physiologically active substances in which the physiologically active substances are expressed using any of the pGbc, pGbcJL and pGbc_L and pGbc_S vectors in paras. 1-3. 5. Способ по п. 4, в котором физиологически активные вещества экспрессируются в клетках, происходящих из ткани молочной железы, которые трансфицированы векторами pGbc_L или pGbc_S.
6. Способ по п. 4, в котором физиологически активные вещества экспрессируются в трансгенных животных с помощью вектора pGbc.5. The method of claim 4, wherein the physiologically active substances are expressed in cells derived from breast tissue that are transfected with the vectors pGbc_L or pGbc_S.
6. The method of claim 4, wherein the physiologically active substances are expressed in transgenic animals using the pGbc vector. 7. Способ по п. 5, в котором указанные клетки, происходящие из ткани молочной железы, являются клетками линии НС11. 7. A method according to claim 5, wherein said cells derived from breast tissue are cells of the HC11 line. 8. Способ по п. 7, в котором клетки НС11 подвергаются трансфекции несущим ген вектором pGbc_L или вектором pGbc_S с помощью метода кальций-фосфатного соосаждения или электропорации. 8. The method of claim 7, wherein the HC11 cells are transfected with the gene-carrying vector pGbc_L or the vector pGbc_S using the method of calcium phosphate coprecipitation or electroporation. 9. Способ по п. 6, в котором получают трансгенных животных с помощью микроинъекции несущего ген вектора pGbc в мужской пронуклеус животных. 9. The method of claim 6, wherein the transgenic animals are obtained by microinjection of the pGbc vector-carrying gene into the male pronucleus of animals.
RU2000115566/13A 1998-09-11 1998-09-11 RECOMBINANT VECTOR USABLE IN SYNTHESIZING PHYSIOLOGICALLY ACTIVE SUBSTANCE CONTAINING PROMOTOR SITE OF β-CASEIN GENE OF KOREAN LOCAL GOATS AND METHOD FOR PERFORMING THE PHYSIOLOGICALLY ACTIVE SUBSTANCE RU2191826C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KRPCT/KR98/00277 1998-09-11
PCT/KR1998/000277 WO2000015808A1 (en) 1998-09-11 1998-09-11 MAMMARY GLAND TISSUE-SPECIFIC EXPRESSION SYSTEM USING $G(b)-CASEIN PROMOTER SITE OF KOREAN NATIVE GOAT

Publications (2)

Publication Number Publication Date
RU2000115566A true RU2000115566A (en) 2002-05-27
RU2191826C2 RU2191826C2 (en) 2002-10-27

Family

ID=19531117

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2000115566/13A RU2191826C2 (en) 1998-09-11 1998-09-11 RECOMBINANT VECTOR USABLE IN SYNTHESIZING PHYSIOLOGICALLY ACTIVE SUBSTANCE CONTAINING PROMOTOR SITE OF β-CASEIN GENE OF KOREAN LOCAL GOATS AND METHOD FOR PERFORMING THE PHYSIOLOGICALLY ACTIVE SUBSTANCE

Country Status (10)

Country Link
US (2) US6635474B1 (en)
EP (1) EP1034281B1 (en)
AT (1) ATE289352T1 (en)
AU (1) AU729668B2 (en)
DE (1) DE69829069T2 (en)
ES (1) ES2237849T3 (en)
HU (1) HUP0102530A3 (en)
NZ (1) NZ505218A (en)
RU (1) RU2191826C2 (en)
WO (1) WO2000015808A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100408429B1 (en) * 2000-01-24 2003-12-06 한미약품 주식회사 Transgenic goat producing milk containing human granulocyte-colony stimulating factor
KR100790514B1 (en) * 2005-08-26 2008-01-02 (주)엠젠 A transgenic pig expressing CSF gene in the milk and producing method thereof
KR100912038B1 (en) 2007-12-31 2009-08-12 전남대학교산학협력단 Porcine ?-casein genomic DNA, and recombinant vectors and transformants containing the same
RU2525712C2 (en) * 2012-07-10 2014-08-20 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук Method for creating transgenic animals with stable and high target protein expression in milk

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993024627A1 (en) * 1992-06-03 1993-12-09 Case Western Reserve University Bandage for continuous application of biologicals
US5827690A (en) * 1993-12-20 1998-10-27 Genzyme Transgenics Corporatiion Transgenic production of antibodies in milk
WO1996040879A1 (en) * 1995-06-07 1996-12-19 Utah State University Dna cassettes for expression of lytic peptides in mammalian cells and transgenic organisms containing same
JPH0937786A (en) * 1995-07-31 1997-02-10 Terumo Corp Promoter and vector containing the same

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