RU2000104205A - METHOD FOR IDENTIFYING IN VITRO EFFECT OF ALLERGIZATION ON INTRODUCTION OF CAPTOPRIL - Google Patents

METHOD FOR IDENTIFYING IN VITRO EFFECT OF ALLERGIZATION ON INTRODUCTION OF CAPTOPRIL

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Publication number
RU2000104205A
RU2000104205A RU2000104205/14A RU2000104205A RU2000104205A RU 2000104205 A RU2000104205 A RU 2000104205A RU 2000104205/14 A RU2000104205/14 A RU 2000104205/14A RU 2000104205 A RU2000104205 A RU 2000104205A RU 2000104205 A RU2000104205 A RU 2000104205A
Authority
RU
Russia
Prior art keywords
captopril
neutrophils
blood
allergization
introduction
Prior art date
Application number
RU2000104205/14A
Other languages
Russian (ru)
Other versions
RU2211452C2 (en
Inventor
Юрий Михайлович Лопатин
Олег Владимирович Илюхин
Анатолий Трофимович Яковлев
Дмитрий Викторович Викторов
Михаил Евгеньевич Стаценко
Ирина Борисовна Захарова
Original Assignee
Волгоградская медицинская академия
Filing date
Publication date
Application filed by Волгоградская медицинская академия filed Critical Волгоградская медицинская академия
Priority to RU2000104205/14A priority Critical patent/RU2211452C2/en
Priority claimed from RU2000104205/14A external-priority patent/RU2211452C2/en
Publication of RU2000104205A publication Critical patent/RU2000104205A/en
Application granted granted Critical
Publication of RU2211452C2 publication Critical patent/RU2211452C2/en

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Claims (1)

Способ выявления in vitro эффекта аллергизации на введение каптоприла, основанный на оценке усиления амебоидной активности нейтрофилов при инкубации крови сенсибилизированного организма с специфическим антигеном (каптоприлом): в опытную пробирку вносят 0,1 мл аллергена в необходимой концентрации, в контрольную 0,1 мл 5% цитрата натрия, затем в обе пробирки добавляют 0,4 мл исследуемой крови, инкубируют 2 ч при 37oС и мазки крови окрашивают последовательно реактивом Шифа и гематоксилином Эрлиха, показатель повреждения нейтрофилов определяют по формуле - (H1-H2):100, где H1 - количество поврежденных нейтрофилов в опыте, H2 - в контроле, 100 - просмотренных в мазке, за диагностическое значение принимают показатели от 0,1 и выше.A method for detecting the in vitro allergy effect on the administration of captopril, based on the assessment of the enhancement of the amoeboid activity of neutrophils during incubation of the blood of a sensitized organism with a specific antigen (captopril): 0.1 ml of allergen in the required concentration is added to the test tube, 0.1 ml of 5% to the control sodium citrate, and then both tubes were added 0.4 ml of the investigated blood, incubated for 2 hours at 37 o C and blood smears were stained sequentially with hematoxylin and reagent Schiff Ehrlich, neutrophils damage index is determined by formula is - (H 2 -H 1) 100, where H 1 - the number of neutrophils in damaged experiences, H 2 - in the control, 100 - viewed in the smear for receiving diagnostic value indicators 0.1 and above.
RU2000104205/14A 2000-02-18 2000-02-18 Method for in vitro detecting allergization phenomena on administering captopryl RU2211452C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2000104205/14A RU2211452C2 (en) 2000-02-18 2000-02-18 Method for in vitro detecting allergization phenomena on administering captopryl

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2000104205/14A RU2211452C2 (en) 2000-02-18 2000-02-18 Method for in vitro detecting allergization phenomena on administering captopryl

Publications (2)

Publication Number Publication Date
RU2000104205A true RU2000104205A (en) 2001-10-27
RU2211452C2 RU2211452C2 (en) 2003-08-27

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU2000104205/14A RU2211452C2 (en) 2000-02-18 2000-02-18 Method for in vitro detecting allergization phenomena on administering captopryl

Country Status (1)

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RU (1) RU2211452C2 (en)

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