RU199874U1 - DEVICE FOR ASSESSING "ACUTE" TOXICITY OF BIOLOGICALLY ACTIVE COMPOUNDS ON SMALL LABORATORY ANIMALS - Google Patents

Abstract

The utility model relates to the field of experimental biology and pharmacology, namely to devices that can be used to assess the "acute" toxicity of biologically active compounds in small laboratory animals - mice. The device is made of polymethyl methacrylate in the form of a container with five compartments, with the front, rear, inner side walls and the lid being perforated, and in the upper part of the rear wall there are five latches. The proposed utility model is aimed at improving the quality and convenience of observation of laboratory animals during the first hours after exposure to the investigated factor, which increases the reliability of the results obtained during toxicological studies. 3 ill.

Description

The utility model relates to the field of experimental biology and pharmacology, namely to devices that can be used to assess the "acute" toxicity of biologically active compounds in small laboratory animals - mice.

Conducting toxicological studies is necessary to evaluate any biologically active substances, in particular, this is a mandatory section of studies of potential drugs. Such studies make it possible to establish a hazard class in accordance with GOST, the nature and severity of the damaging effect of biologically active substances on the body of experimental animals, to identify organs and body systems sensitive to the effect of the factor under study, and to study the reversibility of the damage caused. Particularly informative in assessing "acute" toxicity are the first hours after exposure to the investigated factor.

Known device for keeping laboratory animals (SU 519174), contains a cage, equipped with a manhole, rigidly attached to the camera body, lattice bottom, valve and feeder with an inclined hopper.

The disadvantages of this device are the inconvenience of using and observing the animal, the device is intended for keeping mainly rats, the experimental animal has access to food, which affects the results of the studies.

Known device "Cage for animals" (RU 161632), consisting of walls, a cover with a handle, a base on which a cruciform partition is fixed, dividing the cage into 4 parts. The cover is secured with wing nuts screwed on top of the cover.

The disadvantage of this device is the complexity of the design, the inconvenience and impossibility of long-term observation of animals, the insufficient number of holes for the normal functioning of the vital activity of a living organism.

The prototype of the proposed utility model is T-3 plastic cages for keeping laboratory animals for assessing "acute" toxicity, described in RU 2641380; Sorokina A.V., Alekseeva S.V., Nemova E.P. et al. Preclinical study of the safety of the GB-115 dipeptide compound // Experimental and Clinical Pharmacology. - 2010. - T. 73. - No. 6. - S. 29-32; Ivaschenko A.V., Yamanushkin P.M., Mitkin O.D. et al. Preclinical study of the safety of the new pharmacological substance AV0012 for the treatment of hepatitis C // Experimental and Clinical Pharmacology. - 2014. - T.77. - No. 12. - S. 33-37.

The disadvantage of the prototype is the inconvenience of use, limited observation of the reaction of the animal in response to the action of the investigated factor.

The technical result of the claimed utility model is the creation of a device for accommodating small laboratory animals - mice for several hours, which ensures the convenience of monitoring the state of the animal in the first hours after exposure to the investigated factor, which significantly increases the information content in assessing "acute" toxicity and the quality of the experiment.

The specified technical result is solved in that it is made of polymethyl methacrylate in the form of a container with five compartments, and the front, rear, inner side walls and the lid are perforated, and in the upper part of the rear wall there are five clips.

The utility model is illustrated with a detailed description and illustrations, which show:

FIG. 1 - appearance of the device: a) 1- base; 2- front wall; 3- back wall; 4- inner side walls; 5- retainer; 6 - cover; 7- through hole; b) photo of the device, 1- base; 2- front wall; 3 - back wall; 4 - inner side walls; 5 - retainer; 6- cover; 7 - perforation; c) photo of the device, top view; 1- base; 2- front wall; 3- back wall; 4- inner side walls; 5- retainer; 6 - cover; 7- perforation.

FIG. 2 is a diagram of the device with dimensions; a) rear view; b) top view; c) side view; d) fragment A - rear view; e) fragment B - side view.

FIG. 3 - photo of device use: a) side view; b) top view.

The device is a container made of organic glass - polymethyl methacrylate with five compartments (Fig. 1 a) and consists of a base 1, a front wall 2, a back wall 3, a cover 6 and four inner side walls 4 to create compartments (Fig. 1 a, b , at). On the upper part of the back wall of the container there are locks 5, which ensure a secure fit of the lid. All walls and lid are made with perforation 7 (for the normal functioning of the vital functions of experimental animals).

The claimed device for assessing the "acute" toxicity of biologically active compounds in small laboratory animals is used as follows.

The device (Fig. 1 a, b, c) is installed on the laboratory table in the working room. Filter paper is placed on the base 1 to visualize the reactions of the experimental animal in response to the influence of the investigated factor. After the introduction of the test substance in a certain dose, 2 animals weighing up to 27 g are placed in the compartment of the container. After all manipulations, the container is covered with a lid 6.

The device allows you to assess the effect of the investigated factor applied in 5 variants / doses simultaneously, using up to 10 animals. The ease of use of the device allows one person to be involved in an experiment.

Execution example.

The indicators of "acute" toxicity of the compound were determined under the code ITM-5 by the express method according to V.B. Prozorovsky on 8 outbred male mice weighing 24-27 g (Figure 3). This device made it possible to minimize the number of animals to determine the value of LD ₅₀ - the average effective dose that causes the death of 50% of the individuals in the study group.

To carry out the experiment, a device was made of organic glass - polymethyl methacrylate with five compartments (Fig. 2 a, b, c, d, e). The cover 6 was removed from the device (Fig. 1 b, c; Fig. 3) and filter paper was placed on the bottom of each compartment. The test compound was administered to animals intraperitoneally at doses of 316, 398, 501, 631 mg / kg. After the introduction of ITM-5, the animals were placed in the compartments and covered with a lid. During the first five hours, observation was carried out, assessing and recording the state of the animals: hyperkinesis of varying severity, up to death. At the end of the observation during the first five hours, the surviving individuals were placed in cages with free access to food and water for further observations for 14 days. The filter paper was removed from the container, and the device itself was cleaned with detergent. According to the results of the study, the average effective dose and the corresponding range of deviations from the mean value were calculated. For the ITM-5 compound, the LD ₅₀ value turned out to be 410 (268 ÷ 552) mg / kg.

The use of the device in the experiment allowed:

- to achieve reliable isolation of small laboratory animals within a few hours;

- to improve the quality of the experimenter's work and the accuracy of the description of toxic reactions;

- reduce the stress of laboratory animals.

- to provide good aesthetic properties, transparency and reliable isolation of experimental animals from each other.

The proposed utility model is aimed at improving the quality and convenience of monitoring laboratory animals during the first hours after exposure to the investigated factor, which increases the reliability of the results obtained in the course of toxicological studies. The device is made of organic glass - polymethyl methacrylate. The material provides good aesthetic properties, transparency, and also reliably isolates experimental animals from each other.

The device is simple in design, convenient in operation, reliable in operation, simple in manufacture and does not require large expenditures. The device is industrially applicable, since it can be produced commercially as a kit for experimental biology and pharmacology.

Claims (1)

A device for monitoring small laboratory animals and assessing the "acute" toxicity of biologically active compounds, characterized by the fact that it is made of polymethyl methacrylate in the form of a container with five compartments, and the front, back, inner side walls and lid are perforated for the functioning of the vital functions of experimental animals, and there are five clips on the top of the back wall.

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