RU199321U1 - DEVICE FOR FIXING FLAT BIOLOGICAL MATERIAL INSIDE A STANDARD 120 ML BIOLOGICAL CUP - Google Patents

Abstract

The utility model refers to laboratory equipment of biological and other laboratories and can be used for fixing flat biological tissues in the process of washing them with various solutions, for example, with detergents for decellularization. The mesh material is made in the form of two mirrored trapezoids connected to each other along the lower base, and along the perimeter of the trapezoid there are flat dense plastic edges in the form of a rigid frame and two plastic tubes-fixators with longitudinal cuts connecting the edges of the two mesh trapeziums after they are closed. The proposed device is easy to use, compact and lightweight, which allows it to be used in a standard 120 ml beaker for biological samples and to be washed with any solutions (for example, during decellularization). The device provides a gentle and safe attachment of tissues between two mesh bases, preventing twisting and deformation of tissues, and thereby allows high-quality washing of tissues from all sides, since the sealed edges do not allow the device to bend or move when using a peristaltic pump during tissue washing, and the fixed biological tissue remains motionless throughout the entire washing process. 5 ill.

Description

This utility model relates to laboratory equipment of biological laboratories and can be used for non-destructive and effective washing of flat organs and tissues with any solutions, including detergents for decellularization.

To carry out high-quality and uniform decellularization (removal of cellular components from an organ or tissue by washing with special detergents while preserving the extracellular matrix and the three-dimensional structure of the organ), a firm fixation of the tissue or organ on the surface of the equipment is required. However, a solid surface will not work. will interfere with the penetration of the solution from the attachment side to this surface. For maximum efficiency of the decellularization process, it is necessary that biological tissue be surrounded by a moving stream of detergents from all sides.

Currently, there are a number of devices for fastening tissues and organs, for example, cassettes for fixing biological material or cassettes for preparing biological tissue samples for histological studies. However, their main disadvantages are the impossibility of firm attachment of flat biological tissues to the surface to prevent its twisting and deformation. For the decellularization process, a firm and non-destructive placement of the entire tissue surface is required, providing solutions with maximum access to all biological material.

A useful model is known from the state of the art (RU 140360 U1, 05/10/14), which is a spoon-sieve for brewing tea, which refers to reusable means for brewing tea or other infusions. It includes a body, consisting of two parts, interconnected by a leaf spring with the possibility of abutting one part to another, and when pressed on the holding one of the body parts, they are disconnected from each other into their component parts.

The disadvantage of the device is the spacious compartment in which it is impossible to firmly fix the flat fabric. Also, its disadvantage is the attachment of the device to the wall of the glass, which does not allow the solution to circulate from all sides.

Known utility model (US 5427742, 06/27/1995), which is a cassette for processing small samples of tissue, which includes a rectangular box-shaped base with a perforated bottom wall and a removable perforated cover. There is a round mesh compartment for tissue samples on the base and on the lid. The connection of the base and lid forms a closed area within the circular mesh compartment to retain the tissue specimen during preparation for examination. The external cassette allows processing fluids to flow through the circular mesh compartment and resists the leakage of small tissue samples from it during processing. The outer base frame is rectangular in shape to conform to standard microtome tissue specimen shapes. The round mesh compartment is made of woven nylon material.

The disadvantage of the device is the technical complexity of the design and rare applicability, justified only for some experiments in which small particles of tissue are used. Since in the process of tissue decellularization, a thorough washing of the cellular constituent of the biological material must take place to obtain an extracellular matrix (ECM), deep penetration of solutions to tissues and particles of the biomaterial is necessary.

There is a known method of manufacturing a dermal matrix related to the decellularization of flat tissues and organs (RU 2524619 C2, 07/27/2014). The method comprises the use of an orbital shaker with a variable rotation speed. Hermetically sealed beakers containing the necessary solutions for decellularization with the biomaterial immersed inside are placed on the shaker.

The disadvantage of this method is the unlimited mobility of the biological material with the absence of any fixation, which can directly affect the structure and mechanical properties of the resulting biomaterial.

Known invention for fixing biomaterial - a cassette device with a hinged cover for tissue (US 4549670, 29.10.1985). At the moment, there are a huge number of modifications of the presented device in the world, but they do not represent practical significance for us due to their peculiarities of recording biological material. This tissue cassette device includes a removable hinged cover that is easily attached and detached from the base. The invention is a liquid-permeable cassette adapted to receive a tissue sample and to hold the tissue sample during contact with a treatment liquid.

Disadvantages of the system: difficult to manufacture, small in size, closed enough for thorough washing of biological material.

The closest to our proposed device is the article "Morphological assessment of the quality of decellularization of the heart and diaphragm of rats" (Cell transplantology and tissue engineering, T. VII, No. 4, 2012). The decellularization device used in this article includes a Petri dish to which a mesh is attached. All biological materials placed on it are freely positioned without fixation.

The disadvantage of this device is that when using the standard rotary method, there is no reliable fixation of the biological material. It is possible to place fabrics of various shapes and sizes on the device, but it is not possible to fix them in one position. Loose biological material can be destroyed, which will negatively affect its mechanical properties and density of the ECM. Also, the lack of the possibility of washing (or decellularization) of biological material from all sides can affect the results of the experiment.

The technical result consists in creating a device that is capable of providing a strong fixation of biological material with unhindered access of the washing solution directly to the examined flat tissues and organs inside a standard 120 ml beaker for biological samples.

The technical result is achieved in that, as in the known device, a mesh material is used.

The specified technical result is solved by the fact that the mesh material is made in the form of two mirror trapezoids connected to each other along the lower base, and along the perimeter of the trapezoid there are flat dense plastic edges, in the form of a rigid frame and two plastic tubes-fixators with longitudinal cuts connecting the edges of two mesh trapezoids after they are closed.

The utility model is illustrated with a detailed description and illustrations, which show:

FIG. 1 - diagram of the device: 1 - mesh base in the form of two mirrored trapezoids, 2 - plastic edges with a rigid frame, 3 - fixing tubes with longitudinal cuts.

FIG. 2 - diagram of using the device in a standard beaker for biological samples of 120 ml: 1 - diagram of the device: 1 - mesh base in the form of two mirrored trapezoids, 2 - plastic edges with a rigid frame, 3 - fixation tubes with longitudinal cuts, 4 - beaker 120 ml for biological samples.

FIG. 3 - photo of the device: general view.

FIG. 4 - photo of the device: fixation of the mesh base in the form of two mirrored trapeziums with fixing tubes.

FIG. 5 - photo of the device: an example of using a utility model in a standard 120 ml beaker for biological samples.

Description of the utility model.

The device (Fig. 1, 2) consists of two mirrored trapezoids connected to each other along the lower base 1, along the perimeter of the trapezoid there are flat dense plastic edges, in the form of a rigid frame 2 and two plastic tubes-fixing with longitudinal cuts 3, connecting the edges of two mesh trapezoids 1 after their closure.

In the implementation of the utility model, a device has been created for fixing flat tissues and organs of laboratory animals in a standard 120 ml beaker 4 for biological samples.

The device is made (Fig. 3, 4, 5) of a durable and lightweight mesh material in the form of two mirror trapezoids connected to each other along the lower base 1 (mesh size 2 × 2 mm). The edges of the trapezium 1 are sealed with plastic 2 to make the structure rigid. The length of the lower base of each of the trapeziums is 45 mm, the upper one is 55 mm. Height of the device is 120 mm. The hard edges 2 are made of a 75 micron double layer of laminating film. The edges are 7 mm wide. When folding the mesh 1, the edges are connected with 4 mm clips with longitudinal cuts on one side 3.

The device (Fig. 3, 4, 5) is suitable for firm but safe attachment of tissues without damage. The biological tissue is placed in the center of one of the bases 1, then it is covered with a second base, connected to the first on the lower side. Next, using the tubes-clips 4, the edges of the trapeziums 1 are connected. The device is then placed in a standard 120 ml biological sample beaker 4.

The proposed device is easy to use, compact and lightweight, which allows it to be used in a standard 120 ml beaker for biological samples and to be washed with any solutions (for example, during decellularization). The device provides a safe and non-destructive fixation of tissues between two mesh bases, preventing twisting and deformation of tissues, and thereby allows high-quality washing of tissues from all sides, since the sealed edges do not allow the device to bend or move when using a peristaltic pump during tissue washing , and the fixed biological tissue remains motionless throughout the entire washing process.

Claims (1)

A device for fixing a flat biological material inside a 120 ml glass for biological samples, including a mesh material, characterized in that the mesh material is made in the form of two mirror trapezoids connected to each other along the lower base, and along the perimeter of the trapezoid there are flat films made of a double layer for lamination, plastic edges, in the form of a frame and two plastic retaining tubes with longitudinal cuts on one side, connecting the edges of the two mesh trapeziums after they are closed.

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