RU1824440C - Method for cultivating microorganisms - Google Patents

Abstract

Usage: microbiologists. SUMMARY OF THE INVENTION: A method for cultivating microorganisms includes growing on media containing carbon, nitrogen, phosphorus, mineral salts and a growth promoter, the product of microorganisms synthesizing bacteriorhodopsin. A new method is the use of a liquid fraction to stimulate the growth of microorganisms obtained in the process of isolating bacteriorhodopsin from disintegrates of bacteriorhodopsin synthesizing cells, which is a waste product. 3 tab.

Description

The invention relates to microbiology, in particular to methods of cultivating microorganisms, and can be used in the cultivation of various groups of bacteria.

The purpose of the invention is to increase the yield of biomass, reducing its cost,

This is achieved by the fact that in the cultivation of microorganisms on a nutrient medium containing sources of carbon, nitrogen, phosphorus, mineral salts and a growth promoter, non-bacteriorhodopsin is used as a growth promoter. and the waste of its production is the liquid fraction obtained in the process of isolating bacteriorhodopsin from lysates of producer cells.

The essence of the method is. that microorganisms are cultured using media prepared by using a stimulant which is a raspberry colored liquid with a typical dry matter content of about 1.5%. The stimulator contains. It contains the remains of the cells of microorganisms producing bacteriorhodopsin, which remain after the isolation of bacteriorhodopsin from the cells, water, and the contents of the cells. The stimulant is a homogeneous, transparent liquid with a pleasant odor. It can be sterilized in an autoclave at a vapor pressure of 1 atm while maintaining its properties. At the indicated vapor pressure, a sufficient sterilization time of 20 minutes (120 ° C). The stimulant is easily diluted with water and any ratios. To achieve the maximum effect, a complete change of water is allowed when preparing media for the indicated stimulator.

Example. Thermophilic methylochrophic bacteria, strain T-45 are grown in flasks on a rocking chair on a nutrient medium prepared with a mixture of stimulant and BOC

with

oo

N3 Јь Јь О

dy (1: 1) (the stimulator contains 1.5% dry matter and was obtained by isolating b cteriorodopsin from aqueous lysatopes of Halobacterlum haloblum 353 P cells) of the following composition, g / l: KCI -0.3; MgSCM. 7H20-0.6; FeSCM. 7H20-0.016; ZnSO / i. 7H20 0.011; MnSCM, 5M20-0.12; SZSm. 5H20-0.015; NzRSm - 0.2 ml. The concentration of methanol in the medium is 0.5 volume percent, pH 7.2. Cultivation is carried out at 40 ° C. The biomass yield is 0.5 g / l ASB. When grown according to the prototype, its yield is 0.3 g / l.

From the data obtained it can be seen that according to the proposed method, the biomass yield is 66, G% higher.

Example 2. Two media options are used: 1st — nutrient agar CD (Stavropol Research Institute of Vaccines and Serums) prepared on distilled water; 2nd — CD nutrient agar prepared with a mixture of distilled water and liquid waste from the production of bacheroriodopsin. containing 1.5% dry matter, the ratio of the components is 1: 1. According to the prototype, for the 1st embodiment, the medium was used as a stimulant. Growth of bzterioriodopsin at a concentration of 0.005%, and illumination was also carried out for 25-30 minutes after sowing cultures with an intensity of 10 erg / cm s. All cultures were seeded with an injection of a microbiological needle deep into agar plates and cultured at 37 ° C for 1 day. Then, the diameter of the colonies was measured. The application of the proposed method, compared with the prototype, gives a greater effect (table 1).

Thus, the proposed method has a large positive effect on capsular and capsule-free microbes of the intestinal group.

Example 3. Analogously to example 2. The stimulating response is checked on epiphytic and phytopathogenic microorganisms, the macrocolonies of which are grown on agar medium, including a mixture of potato broth and liquid waste from bacteriorhodopsin production (1: 1) - option 2, and option 1 - ordinary potato agar.

EXAMPLE 4. Analogously to Example 2. The stimulatory effect is tested on spore microorganisms.

Thus, the proposed method has greater productivity than the known one, by a value of from 10 to 200%. In addition, it has a lower cost, since the quality of the stimulator use liquid waste from the production of bacteriorhodopsin.

Claims (1)

The claims A method of cultivating microorganisms by plating on a nutrient medium containing a source of carbon, nitrogen, phosphorus, mineral salts and a growth promoter, a product of bacteriorhodopsinsynthesizing microorganisms. that, in order to increase the yield of biomass and reduce the cost of the method, as a quality. The growth promoter utilizes the waste product of bacteriorhodopsin production - the liquid fraction obtained by isolating bacteriorhodopsin from disintegrates of bacteriorhodopsin-synthesizing microorganisms. The stimulating effect of liquid waste production bacteriorhodopsin capsule and capsuleless gut microbes Table 1 Note: d (mm) - average colony sizes in diameter, rounded to the nearest interest is up to whole. The effect of liquid waste from the production of bacteriorhodopsin on bacteria living on plants Note: d (mm) is the average diameter of the bacterial colonies after 3 days of cultivation at 28 ° C, rounded to the tenth, and percentages to whole. table 2 Stimulating effect of liquid bacteriorhodopsin on spore germs Note: d (average colony sizes in mm after 1 day of cultivation at 28 ° C. The percentages are rounded to the nearest whole; the sizes of the colony diameters are up to ten. Table 3