RU1805850C - Fungi race bipolaris sorokiniana (sacardo rier andrea) shoemaker for evaluation of spring wheat resistance to root rot - Google Patents

Abstract

Usage: agriculture, selection for root wheat resistance to root rot and relates to a new strain for evaluating wheat for root rot resistance. The inventive strain of the fungus Bipolaris Sorokiniana (Saccardo, Pier Andrea) Shoemaker isolated from root rot affected flat wheat tissue in the tillering phase, deposited in the combined collection of microorganisms VNI-ISKHM-VIZR under No. D-46. For inoculation of durum wheat, the strain was grown on a grain mixture at a temperature of 26 ° C for 15-20 days. 30 to 40 g of inoculum per meter are used for inoculation. The pathogenicity of the strain is 63-75%. 2 tab. with

Description

The invention relates to agriculture and is a new strain of fungus intended for use in breeding for the resistance of flat wheat to common root rot and can be recommended as a typical phytopathogenic strain.

An object of the invention is to provide a strain of the fungus Bipolaris sorokiniana. meeting the requirements for assessing, on infectious backgrounds, breeding material of durum wheat for resistance to common root rot: stability of cultural and morphological properties, good growth and sporulation on media,

high pathogenicity, the ability to maintain their properties when replanting on agar media and in the soil.

The proposed strain Bipolaris sorokiniana was isolated from the root rot of tissues of the durum wheat cultivar Novosibirsk 81 in the tillering phase in 1985 and is stored in the combined collection of microorganisms VNIISCHM-VIZR under the number D 46.

To obtain such a strain, we studied the isolates of the fungus Ripolarls sorokiniana from zoned and promising varieties of flat wheat in different phases

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twigs and under different growing conditions.

Strain D 46 has the following morphological, physiological and pathogenic characteristics.

1. On Chapek’s agarized medium, on the fifth day, colonies reach 5.9 cm in diameter. The aerial mycelium is velvety, even, dark gray. On the underside of the colony are black, s. slightly noticeable zoning. Sporulation on the 10th day is plentiful. Conidia with 1-7 septa (13.3-26.7 x 20.0-70.0 mmc).

On the fifth day of potato-glucose agar, colonies reach 7.3 cm in diameter. Aerial mycelium is felt creeping with appreciable radiality. On the edge of the colony a black rim of sporulation. On the underside of the colony are dark gray with a radial dark color. With the growth of the colony on the 10th day, sporulation in the center of the colony is weak, average along the edge. Conidiis 2-10 septa (10.0-26.7 x 20.0-73.4 mmc).

On soil agar, conidia are rare, large, homogeneous with 3-7 septa (13.3-23.3x33.3-70.0 mkm).

Sporulation is most abundant on an agar medium with maltose.

2. It is well absorbed by sucrose, glucose, lactose, galactose, maltose, potassium nitrate, worse - urea; poorly assimilates sources of nitrate nitrogen.

3. It has a high pathogenicity. The defeat of seedlings in laboratory experiments and plants on infectious backgrounds is 100% with the development of the disease in the laboratory 63-75%, in the prototype 52%, and in the field 56-85%. Inhibition of sprouts was observed in laboratory experiments from 6 to 13 cm in comparison with the control. The study was conducted according to compiled methods where the essence of the proposed strain is not disclosed.

Equal wheat seeds inoculated during the formation of the caryopsis reduce laboratory germination in comparison with the control by 25-66%.

4. Highly toxic. The growth and development of seedlings of flat wheat is approaching

to the control option when diluting the culture fluid is not less than 1: 100.

5. Viable. When sowing on agar

The cap and storage at a temperature of 5-7 ° C retains its pathogenic properties for 6 or more years (Table 1).

Therefore, the proposed strain VNIISKHM-VIZR N D 46 has a high

pathogenicity: the development of the disease in laboratory experiments 63-75%, the prototype 52%, and retains its pathogenic properties for 6 or more years.

When creating artificial infectious backgrounds, the concentration of the fungal suspension in laboratory experiments is 6 thousand hours of conidia, while inoculating ears with a vacuum device, 8 thousand hours of conidia in 1 ml of water. Mushroom cultivation

was carried out on an agarized Chapek medium at a temperature of 26 ° C for 10-15 days. A fungus grown on a grain mixture for 10-20 days at a temperature of 26 ° C was applied to the soil, at the rate of 30-40 g

inoculum per 1 square meter.

On infectious backgrounds using the proposed strain VNIISKHM-VIZR No. D 46, an assessment is made of the resistance and endurance to common rot of durum wheat variety samples from competitive variety testing and the selection of lines from hybrid material.

Table 2 shows the yields against the infectious background released by resistance

to ordinary rot of varieties and lines of flat wheat.

Thus, on an infectious background using the proposed strain

VNIISKHM-VIZR No. D 46 isolated varietal samples and lines of flat wheat, resistant to ordinary rot with a high yield exceeding standard varieties. .

F. invention claims Strain of the fungus Bipolaris sorokiniana (Saccardo, Pier Andrea) Shoemaker VNIISCHM-VIZR No. D-46 for evaluating the resistance of root wheat to root rot. .

Table 1

table 2

Varietal sample. Line No. Lutescens wheat line 4579 / p 6028

Lutescens 4148 / and 2096 M 318/88 Ne 289/88 No. 369/88 N 312/8

Novosibirsk standard 67 Novosibirsk standard 81 Irtyshanka standard 10 Rock standard

give birth, g / m, 1990, 264.0

291.0 433.2 421.1 363.0 341.7 168.6 191.7 253.6 201.6