RU1801215C - Method of frequent respiratory diseases prognosis in infancy - Google Patents

Abstract

The invention relates to medicine, namely to pediatrics, and can in particular be used to predict recurrent acute diseases at an early age. The aim of the invention is to improve the accuracy of the method. This goal is achieved by determining the immunological state of the organism, where the antigens of loci A, B of the HIA system in the umbilical cord blood are detected and, in the presence of one of the HIA antigens - A32, B12, B21, B27 or their combinations, frequent respiratory diseases in young children are predicted .

Description

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The invention relates to medicine, namely to pediatrics, and can be used, in particular, to predict recurrent acute respiratory diseases at an early age.

The aim of the proposed method is to increase its accuracy.

The proposed method eliminates the individual fluctuations of the studied parameters, frequent respiratory diseases are predicted already at the birth of a child, which makes it possible to carry out targeted prevention of respiratory pathology in a timely manner, eliminating the need to re-examine the HIA system antigens, since throughout life their persistence; a long-term prognosis of frequent respiratory diseases for the first 2–3 years of life is carried out, infection of the child with hepatitis B or HIV infection is excluded, and psychological trauma is excluded for the child.

This object is achieved in the invention in that antigens of loci A, B of the HIA system are determined in cord blood and, in the presence of one of the HIA-A32, B21, B27 antigens or a combination thereof, frequent respiratory diseases are predicted at an early age.

The invention is illustrated by the following examples.

PRI me R 1. History of childbirth No. 752. Child M., 29.02.89 years of birth, from 1 pregnancy, 1 urgent birth. Mothers are 20 years old. Pregnancy and childbirth without features, body weight at birth 3100 g, The forecasting method was carried out as follows: to 3 ml of heparinized umbilical cord blood (50 IU of heparin per 1 ml of blood) was added an equal volume of Hanks medium and layered Pasteur's writing

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easy in a centrifuge tube with 2.5 ml of ficoll-verographin mixture (mixture density 1,076-1,077). The tube was centrifuged at 200 for 30 minutes, then a white cloud of lymphocytes formed in the interphase was aspirated with a Pasteur pipette. 2 volumes of Hanks medium were added to the lymphocytes, centrifuged again at 200 for 10 min, the supernatant was removed, and the pellet was resuspended in 0.3-0.5 ml of Hanks medium, adjusting the lymphocyte concentration to (2-4) 106 in 1 ml The prepared lymphocytes were instilled in 1 μl into the wells of the Terasaki tablet, in which 1 μl of test sera were previously unearthed. The plate was shaken and incubated at 22 ° for 60 minutes. Next, 5 µl of the rabbit component was added to each well, re-incubated at 22 ° for 60 minutes, after which 3 µl of a 5% aqueous solution of eosil was instilled into the wells. After 10 min, excess paint was removed by stitching and the reaction was read under a microscope at a magnification of 30x100. The reaction was scored based on counting dead (stained) and living (unpainted) lymphocytes as follows:

the number of dead cells up to 10% - About points;

the number of dead cells 11-20% - 1 point;

the number of dead cells 21-50% - 2 points;

the number of dead cells 51-75% - 3 points;

the number of dead cells 76-100% -4 points.

The result was considered positive if it was rated 2 points or more. The set of HIA system antigens in a child was: A32 B13 C 2. Starting at 6 months. In life, the child often suffered from respiratory diseases (6, 8, 10, 11, 13, 15, 16, 19, 20, 21 months of life). Health in group II B is observed and healed. Thus, frequent respiratory diseases in early childhood were predicted for A32 antigen in child M.

PRI me R 2 .. History of childbirth No. 2077. Baby O. 18.04.88 year of birth, from 2 pregnancies, II urgent birth. Mothers are 23 years old. Pregnancy without features, in childbirth a single weak entanglement of the umbilical cord of the fetus. Body weight at birth 3200 g. A method for predicting frequent respiratory diseases by identifying antigens of the HIA system was carried out identically to 1 example. Set of antigens of the child: HIA - A2 B7 BJJ2. Starting from 3 months.

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In life, the child often suffered from respiratory diseases (at 3.5.7, 12.16, 18.19, 21.21, 22, 24, 26 months). Health group III is observed and healed.

Thus, by the presence of B12 antigen, frequent respiratory diseases in child O were predicted.

PRI me R 3. History of childbirth No. 2649. Child G., 08/03/89, born, from II pregnancy, II urgent delivery. Mother 26 years old, pregnancy without features, in childbirth early discharge of amniotic fluid. Birth weight 3650 g. A detailed method for predicting frequent respiratory diseases 5 by determining the HIA antigens is given in the example.

1. A set of antigens of the child: HIA A 10 B21 C

2. From 2 months. of life is observed as often suffering from acute respiratory infections (at 2, 3, 5, 8, 9, 12, 14, 15 months of life). Heals according to group II B health.

Thus, the presence of B21 antigen in a child gave rise to a prediction of frequent respiratory diseases in his early childhood.

PRI me R 4. History of childbirth No. 3652. Child A., 02.10.88 years of birth, from 1 pregnancy, 1 urgent birth. Mother is 22 years old. Pregnancy without features, at birth a threatened rupture of the perineum, lateral perineotomy. Birth weight 3750 g. Prediction method by determining the antigens of the HIA system

5 was carried out identically to 1 example, a set of antigens: HIA A9 A11 B27 C 6. From 3 months of life, respiratory morbidity is frequent (at 3, 4, 6, 9, 13, 14, 17, 18, 19, 22 months of life). Observed and rehabilitated by

0 C B group health.

Thus, the prognosis of frequent respiratory diseases was given to the baby already at the time of the birth of the baby by the presence of B27 antigen.

PRI me R 5. History of childbirth No. 1818. Baby L., born on 22.12.87, from II normal pregnancy, I urgent delivery. Mothers are 26 years old. In childbirth, early discharge of amniotic fluid, weakness of labor, rhodostimulation. Body weight at birth 4100 g. State of the child at birth on the Apgar scale of 8-9 points. At birth, a method for predicting frequent incidence was performed.

5 by determining the HIA system antigens described in Example 1. The A32 B12 B18 antigen kit of the child. From the age of six months, the child has repeated acute respiratory diseases (at 6, 7, 9, 10, 11, 12, 14, 15, 17, 21, 22, 24 months of life), from 12

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months of life are signs of seborrheic eczema. Health group III is observed and rehabilitated. Thus, according to the presence of A32 and B12 antigens in a child, he was given a prognosis for frequent respiratory diseases in early childhood.

Example History of childbirth Mg 4026. Baby V., 11/22/188 years of birth, from 1 normal pregnancy, 1 urgent birth. Mother is 28 years old. In childbirth, early discharge of amniotic fluid. Body weight at birth 3600 g. At birth, predicting frequent respiratory diseases by identifying HIA system antigens is identical to 1 example, a set of baby antigens was: HIA-A2A9B12B27. From 4 months of age, the child often suffered from respiratory diseases (at 4, 7, 10, 11, 12 months of life), and at 7 months of life - an intestinal infection. In the second year of life,

he is ill with less frequent diseases (at 16, 19, 24 months of life), since targeted rehabilitation is carried out with the inclusion of lysozyme, metabolites, and plant adaptogens. Thus, a prognosis of frequent respiratory diseases was given to the baby at birth by the presence of the B12 and B27 antigens.

Claims (1)

SUMMARY OF THE INVENTION A method for predicting frequent respiratory diseases at an early age by examining the immunological status of an organism, characterized in that, in order to increase the accuracy of the method, the antigens of loci A, B of the HIA system are determined in cord blood and in the presence of one of the antigens HIA-A32, B21 , B27, or combinations thereof, predict frequent respiratory infections at an early age.