RU1800361C - Device for chromatographic separation of mixtures in thin sorbent layer - Google Patents

Abstract

Usage: analytical chemistry, analysis of mixtures of substances in various scientific and practical fields of chemistry, medicine, biology, etc. Summary of the invention: the device includes a sealed chamber in which a plate and a container with an eluent are placed. There are three holes in the chamber walls, one of which the space of the chamber is connected to a source of excess gas pressure, the second connects the space of the sorption layer with the atmosphere, and the third is used to introduce the eluent. The device provides for the possibility of stabilizing the speed of the eluent by gradually increasing the gas pressure. 2 s, p. F-ly, 3 ill. fe

Description

The invention relates to the field of analytical chemistry, namely, methods of thin layer chromatography, and may find application in the analysis of mixtures of substances in various scientific and practical fields of chemistry, medicine, biology, etc.

The purpose of the invention is to increase the speed of the chromatographic separation process.

This goal is achieved in that in a device for chromatographic separation of mixtures of substances, which contains a plate with a partially closed polymer film with a thin layer of sorbent having open areas on opposite sides of the plate, one of which is immersed in a container with an eluent, and the other is designed to create a differential pressure on the layer, the plate and the container with the eluent are placed in a chamber that is sealed, with

a removable transparent cover and the housing of which has three openings, respectively designed to connect the chamber space to a source of excess gas pressure, to connect the space of the sorption layer to the atmosphere and to introduce an eluent. The space of the sorption layer is connected to the atmosphere through an opening in the coating by means of an elastic strip and a pressure strip. The chamber is connected to a source of excess gas pressure through a gas flow regulator.

SUMMARY OF THE INVENTION

In FIG. 1 shows a plate intended for use in this device, comprising a substrate 1, a layer of sorbent 2, which is deposited on a part of the surface of the substrate so that on three sides the edges of the substrate are free from the sorbent layer;

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and a polymer coating 3, which is adhered to the surface of the sorbent layer and the substrate and has a slit-like opening located in a direction perpendicular to the proposed direction of motion of the eluent, from the edge of the plate opposite to the sample application site.

In FIG. Figure 2 shows a chamber consisting of a housing 1 and a transparent cover 2, which are sealed with clamping screws 3 by means of an elastic gasket 4 having the shape of a rectangular frame. A plate 5 with a closed sorbent layer is placed inside the chamber, one end of which is immersed in a container with eluent 6. From the opposite end, with the use of a clamping strip 7, elastic seal 8 and screws 9, the sorption layer is connected to the hole 10 in the chamber wall through an opening in the coating which has an outlet to the atmosphere. The chamber is provided with a gas supply opening 11 and an eluent supply opening 12 closed by a membrane 13.

In FIG. 3 is a schematic gas diagram of the apparatus according to the invention, comprising a chamber 1 connected to a pressure source 2 through a pressure regulator 3, a gas flow regulator 4, a switch tap 5 and a buffer tank 6 equipped with a pressure gauge 7.

The chromatographic separation process is carried out as follows. A sample of the mixture to be separated is applied to a portion of the sorption layer of the plate free from polymer coating. The eluent container is placed inside the chamber, the plate is fixed so that one of its ends is lowered into the container, and the slit-like hole in the coating is opposite the hole in the chamber 10 (Fig. 2), after which the lid of chamber 2 is closed and sealed

screws 3. Through the hole 12, the required volume of eluent is introduced into the container 6 using a syringe. Then, using the regulator 3, the required pressure is established and

Using the regulator 4, the required gas flow rate, switch the valve 5 to the position connecting the gas source to the chamber. At the end of the separation process, the tap 5 is switched to its original position, while

Claims (3)

1. Device for chromatographic separation of mixtures of substances in a thin layer sorbent containing a plate with a partially closed polymer film with a thin layer of sorbent having open areas on opposite sides of the plate, one of which is immersed in a container with eluent, and the other is intended to create a pressure differential across the layer, distinguishing e e - in that, in order to increase the expressivity of separation, the plate and container with the eluent are placed in a chamber that is high. filled with a sealed, with a removable transparent cover and the housing of which has three holes, respectively designed to connect the chamber space with a source of excess gas pressure, for connecting the space of the sorption bed with the atmosphere and for introducing the eluent. 2. The device according to claim 1, characterized in that the space of the sorption layer is connected to the atmosphere through a hole in the coating by means of an elastic strip and a pressure strip. 3. The device according to claims 1 and 2, with the exception that in order to improve the quality of separation, the chamber is connected to a source of excessive gas pressure through a gas flow regulator. P. D 10 8 s - 5 / - I Jl 7