RU1797489C - Method of respiration diseases treatment in calves - Google Patents

Abstract

Usage: veterinarian, treatment of respiratory diseases of the body. Summary of the invention: the method consists in the simultaneous use of serum of convalescents and isoquaterine (2-trimethylammonioethyl carbonate), which is administered intramuscularly in a dose of 1.5-2.0 ml with an interval of 48-72 hours before recovery, 2 tablets ate with

Description

The purpose of the invention is to increase efficiency.

This goal is achieved by the simultaneous use of Convalescents serum and isoquaterine (2-trimethylammonium ethyl carbamate), which is administered intramuscularly in the form of a 10% solution in a dose of 1.0-1.5 ml until recovery. Chemical formula: (CH3) zM CH2CHNGMNSOO H20.

Isoquaterine -2-trimethylammonium-ethyl carbamate. The preparation is a fine-grained powder of whitish color with a slight specific odor, non-volatile, readily soluble in water.

The process for preparing 2-trimethylammonium ethyl carbamate is as follows.

To a solution of 44.0 g (0.5 M) of dimethylaminoethylamine. 50.5 g (0.5 M) of triethylamine in 250 ml of dry ether at a temperature of -20 ° are added with stirring 47.3 g (0.5 M) of methyl chloroformate. Stirring of the reaction mixture was continued for another 2 hours, the temperature was raised to room temperature. After filtering the precipitate and evaporating the solvent under reduced pressure, the residue was distilled on a water-jet pump. 26.5 g of colorless methyl 2-dimethylaminoethyl carbamate I are obtained.

To a solution of 22.0 g (0.5 M) methyl 2-dimethylaminoethyl carbamate in 100 ml of acetone, 28.4 g (0.2 M) methyl iodide are added with stirring. The reaction mixture is warmed up and after 15 minutes Osa VI is formed

ABOUT

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doc Boiling and stirring were continued for 2 hours to complete the reaction. After cooling and filtration, 40.5 g (93.8%) of iodide III methyl 2-trimethylammonium ethyl carbamate are obtained, mp 185-188 ° C.

A solution of 14.4 g (0.05 M) of methyl 2-trimethylammonium ethyl carbamate iodide in 50 ml of water is passed through the strongly basic anion exchange resin Amberlit IRA-400 (in on-form). Anioiitis washed with water until. neutral reaction. The combined aqueous solutions were evaporated under reduced pressure. After triple treatment with isopropyl alcohol, 6.4 g (80%) of colorless crystals of isoquaterine IV are obtained as monohydrate. After crystallization from acetone-isopropyl alcohol, a melting point of 220 °.

Spectrum 13C NMR (in D20). 5: 36.6 (CH2NH); 54.3 ((CH3) 3N +; 66.4 ppm (NCH2).

Elemental composition:

Calculated,%: C 43.9; H 9.8; N, 17.1.

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Found,%: C 44.0; H 10.1; N, 17.0.

The method is carried out as follows.

Convalescence serum is prepared according to N.I. Humpback (1981). Before applying the serum, oxytetracycline hydrochloride was added to it at a rate of 10,000 E.D. per 100 ml of serum.

The resulting mixture was administered intramuscularly at a rate of 1.5-2.0 ml / kg of live weight. At intervals of 24-48 hours until recovery. 24-48 hours after the administration of serum of convalescents, isoquaterine is used, which is administered intramuscularly in the form of a 10% aqueous or isotonic solution in a dose of 1.5-2 ml with an interval of 48-72 hours until recovery. In order to prevent recurrence of the disease, injection isoquaterine is repeated once after clinical recovery,

The method is illustrated by examples.

Example Convalescence serum is obtained by the suction method (N.I. Gorban, 1981). To do this, blood is collected from the vena cava from the animals of convalescents. As donors, animals of the same economy that previously had respiratory illnesses are used. To obtain serum, the collected blood was allowed to stand at a temperature of 20-22 ° C for 5-6 hours, and then placed in a refrigerator. The residual serum was poured into sterile vials and penicillin with streptomycin of 500 U / ml was added. Immediately before use, oxytetracycline hydrochloride is added to the serum

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at the rate of 10,000 E.D. per 100 ml of serum. The resulting mixture was administered intramuscularly at a rate of 2 mg / kg animal weight at 48 hour intervals. 24 hours after the first administration of serum, and then every 24 hours before recovery, a 10% solution of isoquaterine in a dose of 1.5 ml is administered intramuscularly. The cure rate was 92.4%, the duration of the disease was 10 days, while with serum convalescents it was 83.4%.

Example 2. The effectiveness of the method is determined depending on the frequency of use of isoquaterine.

A body with a pronounced clinic of respiratory diseases - an increase in body temperature up to 40-41 ° C, refusal to feed, increased pulse and respiration, hyperemia of the nasal mucosa and conjunctiva, purulent nasal discharge - are treated in accordance with the claimed method. Obtaining and using serum convalescents, as in example 1.

24 hours after the first use of serum, a 10% solution of isoquaterine in a dose of 2 ml was administered intramuscularly with an interval of 72 hours until recovery. Animals of the control group used a serum-convalescents containing penicillin with streptomycin at the rate of 500 E.D. in 1 ml.

The treatment efficiency was 100%, the duration of the disease was 10 days. 5 in the control group of animals, they respectively amounted to 83.4% and 11 days.

Example 3. The effectiveness of the claimed method is determined depending on the interval of application of isoquaterine. 0 After the diagnosis of respiratory diseases (bronchopneumonia, para-influenza-3, bronchopneumonia of bacterial etiology of PG-3), complicated by the virus, the calves at the age of 30-40 days are divided into 5 3 groups according to the analogue principle of 10-15 goals. in each and 1 group of the control, which use serum convalescents ..

Obtaining and using serum convalescents, as in example 1.

Additionally, there is an intramuscular injection of a 10% solution of isoquaterine in a dose of 1.5 ml with an interval of 24, 48, 72 hours.

The treatment results are presented in table 1 ..

Example 4. The effectiveness of the claimed and known methods is determined.

Tel t at the age of 30-45 days with a pronounced clinic of respiratory diseases te0

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Lats are distributed according to the principle of analogues in 3 groups: 2 groups - experimental, 1 group - control.

Convalescents serum of all groups is used, as in Example 1.

Bodies of the experimental groups 24 hours after the first administration of the serum of convalescents intramuscularly, before recovery, a 10% solution of isoquaterine is administered at intervals of 72 hours, the first experimental group at a dose of t, 5 ml, the second - 2.0 ml.

The treatment results are presented in table. 2.

Testing of the claimed method in the farms of the Nizhny Novgorod region confirmed its effectiveness. The application of the method allows to reduce the duration of the disease and death, to eliminate relapse of the disease.

Studies were performed on bodies with a pronounced clinical presentation of respiratory diseases. An analysis of the causes of the disease showed.

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that they were due to a complex of reasons.

A decisive condition for the emergence and spread of respiratory diseases was a decrease in the general nonspecific and immunological resistance of the animal organism as a result of various adverse factors associated with malnutrition, microclimate errors, irregular and inadequate diet, high concentration and lack of stock. Against the background of a decrease in the level of nonspecific resistance, the pathogenic effect of various microflora and viruses is enhanced. The role of bacterial microflora (salmonella, prote) and parainfluenza-3 virus has been established in the occurrence of respiratory diseases. Mostly respiratory diseases were due to the simultaneous exposure of bacteria and parainfluenza virus to the body. The polyetiology of the disease caused in most cases relapses of the disease.

The method of treatment of respiratory diseases of the body of the body, providing for multiple simultaneous administration of serum convalescents and an antibiotic, characterized in that, in order to increase the efficiency of the method, after 24-48 hours a method for treating respiratory diseases of calves depending on the interval of use of isoquaterine

after the first administration of serum and antibiotic, and then 48-72 hours after subsequent administrations, intramuscular injections of a 10% solution of isoquasterine (trimethylammonium ethyl carbamate) are carried out in a dose of 1.5-2.0 ml.

Table 1

Table 2 Comparative effectiveness of the claimed and known methods.