RU1792597C - Method for fish sperm stimulation - Google Patents

Abstract

The invention relates to commercial fish farming. The invention can be used1 to stimulate fish sperm. The purpose of the invention is to increase the length of time for translational motion and increase sperm activity. To achieve this chain, prostagladine 11-deoxypro 6 staglandine E (Doprostone), diluted in saline solution for freshwater fish in concentrations from 0.1 to 1.5 µg / ml, has been proposed as a biologically active, stimulating substance. Using the proposed method increases the sperm activity of fish by 4-6 times compared to the clock cycle for untreated sperm. 1 tab. J

Description

The invention relates to commercial fish farming, in particular to methods for artificial fish farming. The invention can be used to stimulate fish sperm.

| In fish farming, there is a known method for stimulating sperm of fish with biologically active substances, namely gibberelin, heteroauxin or its potassium salt, which is precursor | {that latters the treatment of fish sperm with substances.

A known method of improving quality. sperm of fish by treating it with an aqueous solution of potassium chloride. Sperm processing by this method eliminates weak sperm, thereby improving; sperm quality.

. It is also known to use para-benzoic acid, a biologically active substance, to stimulate sperm.

The disadvantages of these methods are the prolonged exposure of sperm to

activating solution and slight activating effect.

The purpose of the invention is to increase the length of time of translational motion and increase sperm activity ...

The invention is carried out as follows.

Prostoglandin EI Doproston-B was diluted with saline for freshwater fish to a concentration of 11-deoxyprostoglandin EI in it up to 1.5 μg / ml. The composition of saline for freshwater fish is: NaCl - 7.5 g, KS - 0.2 g, 0.2 g, NaHCO3 - 0.2 g, distilled water to a liter. The preparation was prepared as follows: we take the initial ampoule of the Doproston-B preparation containing 5 mg / ml 11-deoxyprostoglandin EI and dilute this preparation with saline 10,000 times. Thus, an agent containing 0.5 µg / ml of said prostoglandin EI is obtained. The stimulating effect of the drug is based on that.

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that prostaglandins, being highly active substances, act at the cellular level.

Keep the product in a cool place.

Tests of the proposed agent were carried out in laboratory conditions on the sperm of roach, bream and carp obtained after pituitary injection.

Sperm was filtered off, in a manner generally recognized in fish farming, into dry test tubes, which were placed in a cooled thermos bottle and transported semen from the fish farm to the laboratory. The experiments were set as follows.

A drop of a stimulating solution was applied to a clean, dry glass slide with an eye dropper. A small amount of sperm was added to this solution using a dissecting needle. Sperm quality and stimulant were constant in all experiments. The sampling error and the significance of differences with control were calculated. Sperm activity in septic water was taken as a control. Sperm placed in a stimulator solution is well mixed and incubated for one minute. In the dilution solution, the sperm remains stationary. When water was added to this diluted semen, the sperm became motile. Using an MBI-3 microscope, a magnification of 8x10 was used to observe their behavior under natural or artificial light, depending on the experimental conditions; The Agate stopwatch detected the time of sperm processing, i.e. the period of keeping the sperm in the diluent solution without adding simple water, and also recorded the duration of the sperm progression with an accuracy of 0.2 sec. As a control, the behavior of sperm from the same male in ordinary water without the use of a prostaglandin preparation was used. The quality of the agent was judged by the behavior of the sperm after its treatment with the drug in relation to the duration of the progressive movement of sperm treated with a stimulating solution and untreated sperm. Duration

the translational motion of sperm in untreated sperm was taken by us for 100%.

Example. Carp semen in water was motile 71.3 s. and the sperm of the same male after treatment with the proposed method, the concentration of prostaglandin 0.6 μg / ml remained mobile for 438.8 s, thus: 71.3 s is 100%, and

438.8 s is X%,

from here X -. 4388, 1 °° 615.4%, i.e.

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the duration of sperm movement after sperm treatment with prostaglandin increased by more than 6 times.

The table shows the test results of the proposed method on roach, bream and carp. At high concentrations of prostaglandin from 5000 to 300 µg / ml, the spermatozoa remain motionless and die. When using prostaglandin in concentrations from 300 to

100 µg / ml, 10-20% of all sperm cells become active. In experiments using prostoglandin in concentrations of 10 to 5 µg / ml of solution, 50-70% of all sperm cells become active. AT

In experiments using prostaglandin at a concentration of from 1.5 to 0.1 µg / ml of solution, a significant increase in the period of translational movement of spermatozoa was noted. A further decrease in the concentration of prostaglandin leads to the fact that spermatozoa become mobile already in the diluent solution, which is a negative point, since this leads to a decrease in the period of translational

sperm motions. The best result was obtained from the use of a preparation with a concentration of 0.6 µg / ml of solution. The optimal concentration of prostaglandin is from 0.01 to 1.5 µg / ml of solution. The proposed method allows to increase the length of time of translational motion and increase sperm activity.

Claims (1)

The claims A method for stimulating fish sperm, including the treatment of mature fish sperm before insemination of eggs with a solution of a biologically active substance, aphids, which, in order to increase the length of time of translational movement and increased activity of sperm, a solution of prostaglzndin-11-deoxyprostaglandin EI at a concentration of 0.01-1.5 µg / ml of solution is used as a biologically active substance. The effect of Doprostone - B on sperm activity of fish,