RU1778187C - Method for infecting sheep with siberian plague - Google Patents

Abstract

Usage: veterinary microbiologists, anthrax, control of the activity of biological products. SUMMARY OF THE INVENTION: The culture of the causative agent of anthrax is given orally to sheep in a mixture with sterile, finely ground, dusty soil. The method provides a natural reproduction of the disease and increases its reliability, 3 tables. ё

Description

The invention relates to the field of veterinary microbiology and can be used in research institutions to determine the etiological role of microorganisms and to study the pathogenesis of the disease, as well as in enterprises for the production of specific biological preparations (vaccines, sera) for monitoring their immunological activity.

There is a known method of infecting sheep with anthrax, in which the spore pathogen is fed to the animal with food or rubbed into the mucous membrane of the oral cavity.

The disadvantage of this method is that in vivo keeping animals cannot be given

explanation of the reasons for the occurrence of severe injuries on the mucous membranes during the epizootic period in a large contingent of animals. In addition, under experimental conditions, the local form of anthrax was caused in pigs only if they had a severe trauma to the oral mucosa. In the normal state of the mucous membrane or its mild injury, it was not possible to infect Tkii in any case.

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There is also a method; infection of sheep with anthrax after preliminary trauma to the mucous membrane

cavity and tongue with added glass in the infected animal feed.

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The disadvantage of the Dzhny method is that even in the presence of traumatic injuries of the mucous membranes and the use of a stacked stack, the researchers failed to infect the animals with Siberian animals.

In addition, a method is known for infecting sheep with anthrax by oral infection of animals in various catarrhal conditions and dysbiosis of the digestive system.

The disadvantage of this method is that in vivo it is not possible to explain the etiology of the catarrhal network and dysbiosis of the digestive organs in animals during an epizootic period. In addition, there is no 100% mortality in experimental animals from anthrax with this method in advance (64% with infection through a probe and with pipetting).

The aim of the invention is to increase the efficiency of the infection method.

This is achieved by infecting the 5-6 month old sheep with a suspension containing.

2 billion microbial cells of the pathogen culture,

15 g of sterile, finely ground, dusty soil,

100 mg of water.

In the preparatory phase, before conducting the commission experiment, the three components of the mixture were subtitled:

the amount of sterile, finely ground, dusty soil,

amount of water

the number of microbial cells of anthrax culture.

Subtitling was carried out on 15 rabbits with a live weight of kg and sheep of the Kazakh half-fine breed, at the age of one month with a live weight of kg.

The initial mixture for rabbit infection consisted of 6 ml of water, 1 g of soil to which 500 thousand microbial cells of virulent anthrax were added. During the subtitling of the components of the microbial suspension, 5 experiments were carried out on rabbits. The results of these experiments are shown in table. 1.

When carrying out the subtitling of the components of the microbial suspension in sheep, the initial indicator for oral infection was taken as was

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a new indicator of oral infection of rabbits, which consisted of 30 ml of water, 5 g of sterile, finely ground, dusty soil and 1.5 mlod of microbial cells of virulent, anthrax link culture. The experimental results are shown in table. 2.

As a result of the study, the following infectious doses of the components of the microbial suspension were revealed (Table 3).

Example 1. Flattery for rabbits with a live weight of 3-4 kg was prepared as a suspension consisting of 30 ml of water and 5 g of sterile, finely ground dusty soil. The above mixture was thoroughly mixed and set through the mouth using a probe. 48 hours after the above mixture was given, rabbits were slaughtered, the results of which revealed serous-catarrhal inflammation of the mucous membrane of the small and large intestines, and such spot hemorrhages did not occur at the place of transition of the small to the large intestine. When observing the remaining three rabbits for 10 days, there were no deviations in the physiological state of the animals that were visually observed — they were not observed. The body temperature fluctuated within normal limits.

Example 2. Seven rabbits with a live weight of 4-5 kg were given by mouth a microbial suspension of the spore form of a virulent anthrax in a link culture, at a dose of 1.5 billion microbial cells in 30 ml of water. During a 10 day observation of animals, there were no visible visual clinical dysfunctions of the gastrointestinal tract organs. The appetite of the animals was preserved. Body temperature fluctuated within normal limits. The appearance of the pathogen in the blood was not recorded.

Example 3. A suspension containing 30 ml of water + 5 g of sterile, finely divided dusty soil was prepared for rabbits with a live weight of 4-5 kg on the day of infection, to which 1.5 billion microbial cells of virulent anthrax were added. . The above mixture was thoroughly mixed and set through the mouth using a probe. 1

Appetite in laboratory animals is preserved. An increase in body temperature in a rabbit. 1 g-c. Was lucated on oiopi-e day, which in individual animals reached 39.1 - 41 ° C. Then, a gradual decrease in body temperature to Zb was observed (° C. The appearance of the pathogen in the blood was recorded after 28-3 & hours after infection. The animals died within 8-72 hours from anthrax. The diagnosis was confirmed by microscopic and bacteriological methods.

Example

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Three sheep, one month old, with a live cash register of 25–35 kg, were individually given a suspension through their mouths consisting of 100 ml of water and 15 g of sterile, finely ground dusty soil. Clinical observations of the sheep were carried out for 10 days, and the heat temperature, pulse rate, and respiratory chip were measured, which were within the physiological norm. Visually apparent clinical dysfunctions of the gastrointestinal tract organs were not observed.

Example 5. Three unvaccinated specimens aged 5-6 months, with a live weight of 23-35 kg on the day of infection, were prepared as a suspension consisting of 100 ml of water and 2 billion microbial cells of virulent anthrax in a link culture. The above mixture was thoroughly mixed and asked through the mouth. The appearance of the pathogen in the blood was not recorded. Body temperature was within normal limits. Observations of animals were carried out for 10 days. The experimental sheep did not get sick Siberian call.

Example 6. Six non-vaccines to roasted sheep aged 5-6 months and with a live weight of 25-35 kg on the day of the decision was prepared suspension, consisting of 100 ml of water + 5 g of sterile, fine-grained oln Onna, dust additive whether 2 billion microns of virulent anthrax were mixed. The above mixture was stirred and the pathogen was set to be quoted through a 20-m body body; in some individuals, it pressed to 41.1 - 2 ° С. See from anthrax in the tsov after giving microbial agnosis confirmed by myc and bacteriological tests.

Thus, for animals, the oral form of the microbial suspension of anthrax in a mixed, finely divided howl reproduces the infection of animals.

The method allows both nez and epizootic typhus of the disease, on the basis of which additional preventive measures will be developed.

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Method of infection

through the perseral culture of the pathogen, and with the fact that, no efficiency approaching the nature of infection, they suspect about 2 years of age a suspension of 2 billion microbial glue of the pathogen, 15 g of crushed, dusty

water.

Table The results of subtitling the components of the microbial suspension in rabbits

78187. 6

Koms ol1 Onnoy, dusty soil. To it were added 2 billion microbial cells of virulent anthrax siberia. The above mixture was thoroughly mixed and asked through the mouth. The appearance of the pathogen in the blood was recorded after 20 M hours. The body temperature in individual animals rose to 41.1 - 2 ° C. The appetite of the animals was preserved. Death occurred from the anthrax within the period of 89 hours after giving microbial suspension. The diagnosis is confirmed by microscopic and bacteriological methods.

Thus, only by administering to animals an oral, spore form of a microbial suspension of the causative agent of anthrax in a mixture with sterile, finely ground, dusty soil is it possible to reproduce the oral infection of animals by anthrax.

The method allows to study the pathogenesis and epizootic process of the development of the disease, on the basis of which it will be possible to develop additional preventive measures.

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The method of infection of Siberian sheep

I’ve distinguished by the individual administration of the causative agent culture by the fact that, in order to increase the efficiency of the method by approaching the natural conditions of infection, the sheep are infected 5-6 months old with a suspension containing 2 billion microbial cells of the causative agent culture , 15 g of sterile, finely ground, dusty soil and 100 ml

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table 2

Results of subtesting the components of microbial suspension in sheep

Type of animal Poda (ml) (Soil (g) Microbial cells

Rabbits 30.05.01.5 billion

Sheep100.015.01.7 - 2.0 billion

Table 3