RU1775584C - Camera for cryogenic processing of biological matter - Google Patents

Abstract

Use in medicine for cryoprocessing biological tissues in pieces and slices located on glass slides, as well as for their preservation during transportation. Summary of the invention: the chamber contains a thermally insulated housing for a liquid cryoagent, divided by a vertical partition into two communicated at the bottom of the compartment, equipped plugs. In one of the compartments there is a holder for a biomaterial insert. It is located on the surface of the liquid cryoagent and is made in the form of a disk, in the upper surface of which there is a recess for the insert. 1 ill.

Description

The invention relates to refrigeration and is used in medicine for cryo-processing of biological tissues in pieces and slices located on glass slides, as well as for their preservation during transportation

The purpose of the invention is to increase the shelf life of biomaterial and the possibility of its long transportation.

In the drawing, a front section of a chamber for cryoprocessing biological material

The chamber contains a thermally insulated housing for a liquid cryoagent, divided into two compartments 1, 2 by a vertical partition 3. The compartments 1, 2 communicate with each other in the lower part through the window 4 and are tightly closed from above with plugs 5, b. The compartment 1 contains a biomaterial insert holder 7. The biomaterial insert holder 7 is made in the form of a disk with the possibility of positioning a liquid cryoagent on the surface. The holder 7 comprises a chamber 8 below, closed by a cover 9 with holes 10 and a recess 11 from above, in which the biomaterial insert 12 is tightly mounted. The insert 12 is replaceable and has cells or grooves 13 in which either pieces of biological material or slides with slices are installed. The liner 12 may be of various heights, depending on the desired temperature regime of the tissue processing. Outside, the chamber is closed by a casing 14. The chamber and all its constituent elements, including the casing, are molded from dense foam.

The camera operates as follows.

For processing in liquid nitrogen vapors, slices on glass slides or pieces of biological tissue are inserted into the slots or cells 13 of the insert 12. If necessary, the processing of pieces of tissue directly in the liquid nitrogen is introduced into the chamber

8 holder 7 and cover with a bottom

9 with holes 10. Install the liner 12 in the recess 11. The holder 7 is immersed

(AND

WITH

V4 SL SL 00

4

in compartment 1 and close with a plug 5 In compartment 2 pour liquid and from. which penetrates into compartment 1 and into chamber 8, the holder 7 together with the insert 12 and biological material rises as the compartment 1 is filled with liquid nitrogen to a certain level. The compartment 2 is closed from above by a plug 6. Then the chamber is enclosed in a casing 14.

The proposed cryoprocessing chamber has the following advantage: Relative constancy of the temperature regime is ensured, which makes it possible to transport biomaterial and a long shelf life.

Claims (1)

SUMMARY OF THE INVENTION A chamber for cryoprocessing biological material, comprising: a thermally insulated housing for a liquid cryoagent; biomaterial insert holder mounted in the housing with the possibility of location on the surface of the liquid cryoagent and made in the form of a disk, in the upper surface of which is formed recess for the liner, characterized in that, in order to increase the shelf life of the biomaterial and the possibility of its long transportation, the casing is divided by a vertical partition into two communicated at the bottom of the compartment, equipped with plugs, and the holder is installed in one of the compartments. 6