RU149409U1 - OPTICAL SENSITIVE ELEMENT FOR DETERMINING METAL IONS IN LIQUID MEDIA - Google Patents

Abstract

1. An optical sensor for determining the ionic forms of metals, including an optically transparent substrate and a layer of organic polymer with a chromogenic organic reagent immobilized in the layer, characterized in that the organic polymer is a copolymer of methyl methacrylate, ethyl acrylate and the quaternary salt of dimethylaminoethyl methacrylate, and the layer of organic polymer is bonded to a transparent the substrate by adhesion forces, the mass ratio of the organic polymer and the OR in the polymer layer is in the range from 200/1 to 50 / 1.2. The optical sensor according to claim 1, characterized in that the optically transparent substrate is a glass plate with a thickness of 1.0-1.5 mm. The optical sensor according to claim 1 or 2, characterized in that the chromogenic organic reagent is selected from the group consisting of alizarin red, magneson IREA, ferrosine, nitroso-R salt, arsenazo III and arsazene.

Description

The technical field to which the utility model relates.

The utility model relates to spectrophotometric methods of analysis using sensitive elements (optical sensors), as well as test methods with a visual indication of the analytical signal, as the color change is recorded. More specifically, the utility model provides a method for producing a colorless transparent film-concentrator, which is a copolymer of methyl methacrylate, ethyl acrylate and a quaternary salt of dimethylaminoethyl methacrylate, with a chromogenic organic reagent (OR) immobilized in it, the absorption spectrum of which in the visible region changes when interacting with the ionic form of the metal. The utility model also provides sensitive elements that can be used to determine the presence and / or concentration of ions causing a change in the absorption spectrum of the organic reagent incorporated into the film.

State of the art

In Safavi A., Bagheri M. A novel optical sensor for uranium determination // Analytica Chimica Acta. Vol. 530 (2005). P.55-60 shows that the water-soluble metal indicator alizarin red C, lipophilized in the form of an ion pair with tetraoctylammonium bromide and immobilized on a triacetyl cellulose membrane is suitable for the manufacture of optical sensors giving an analytical signal in the presence of UO ₂ ^{2+ ions} in acetate buffer at pH 5. At a sufficiently high contrast (color change of the sensor from yellow to violet) and complete reversibility (regeneration of 0.01 M hydrochloric acid), the sensor has a long response time (6 minutes), and also pazon linearity (1,70-18,7) × 10 ^-5 M, and the detection limit (5 × 10 ^-6 M) is comparable to conventional spectrophotometric techniques, which is its significant disadvantage.

In Safavi A., Bagheri M. Design of a copper (II) optode based on immobilization of dithizone on a triacetylcellulose membrane // Sensors and Actuators. It was shown in 107 (2005) P.53-58 that dithizone immobilized on a triacetyl cellulose membrane can serve as the basis of an optical sensor for detecting Cu ^{2+ ions} in the presence of a complex ion matrix including Pb ²⁺ , Mg ²⁺ , Ca ²⁺ , Ba ²⁺ , Zn ²⁺ , Ni ²⁺ , Co ³⁺ , Mn ²⁺ , Cd ²⁺ , Bi ³⁺ , Fe ³⁺ and Al ³⁺ , with a linearity range of 52.7-1034 ng / ml. The time to reach 95% of equilibrium absorption is 5-8 minutes, which is a disadvantage of the proposed sensor.

In the article, Gavrilenko N.A., Saranchina N.V. Analytical properties of 1- (2-pyridylazo) -naphthol immobilized in a polymethacrylate matrix // ZhAH, T.64 No. 3 (2009). P.243-247 shows that 1- (2-pyridylazo) -naphthol (PAN) sorbed by a polymethacrylate plate from a water-ethanol solution retains its analytical properties, which makes it possible to obtain optical sensors based on it that are sensitive to divalent Ni, Zn ions , Cu, Mn, Cd, Cu, while the sensitivity coefficient within the linearity range of the calibration function (0.05, 1.0 mg / L) is 0.11-1.0 dB · L / mg. The disadvantage of the described system is the long response time (10-60 minutes until equilibrium is established), as well as a fatal systematic error of the order of 0.15 dB, which significantly increases the detection limit of ions.

In the article Aigner D., Ungerboeck B., Mayr T., Saf R., Klimant I., and Borisov S. M. Fluorescent materials for pH sensing and imaging based on novel l, 4-diketopyrrolo- [3,4-c ] pyrrole dyes // Mater. Chem. C. Mater. Opt. Electron Devices. Vol. 1. No.36 (2013). P.5685-5693 described fluorescent sensors for pH measurement, having as a hydrogel sensor film Eudragit ^® RL100 (Evonik Industries, Germany) modified diketopirrolnymi dyes about 7 microns in thickness and coated on an opaque substrate made of Mylar material, and a method for their preparation . The disadvantage of this material is that the proposed films have a specific field of application - only for measuring the pH of aqueous solutions and are not intended to determine metal ions.

In article Naader A., Ali M., Mojtaba S. Poly (vinyl chloride) -membrane ion-selective bulk optode based on l, 10-dibeyzyl-1,10-diaza-18-crown-6 and 1- (2- pyridylazo) -2-naphthol for Cu ²⁺ and Pb ²⁺ ions // Anal. chim. acta. Vol.464. No.2 (2002). P.187-196 proposed an ion-selective optical sensor for detecting Cu ²⁺ and Pb ^{2+ ions} in aqueous solutions. The sensor is based on a plasticized polyvinyl chloride membrane containing 1,10-dibenzyl-1,10-diaza-18-crown-6 (ionophore), chromoionophore-PAN, and tetraphenylborate Na. The determination is carried out at pH 5, detecting Cu ²⁺ and Pb ²⁺ by the optical density of the membrane at 530 and 467 nm, respectively. The sensor allows the determination of Cu ²⁺ and Pb ^{2+ ions} at concentrations of 5.0 · 10 ^-7 ÷ 5.0 × 10 ^-5 M and 1.0 × 10 ^-8 ÷ 5.0 × 10 ^-5 M, respectively. The determination of Cu ²⁺ and Pb ²⁺ does not interfere with Co ²⁺ , Ni ²⁺ , Zn ²⁺ , Hg ²⁺ , alkali and alkaline earth metals. The disadvantage of the proposed sensor based on a polyvinyl chloride membrane is that the optical sensor can detect only two of these cations.

In the article Reshetnyak E.A., Asmolov V.E., German N.N., Nikitina N.A. Optical sensor based on cured gelatin gel for photometric determination of sulfates in aqueous media // Bicnuk of Kharkiv National University. Chemistry. Vip. 18 (41). 2010. No. 895. P.74-81 proposed a sensitive element, the principle of which is based on the formation of BaSO ₄ in solution and the determination of excess barium by reaction with OR nitrochromazo at a wavelength of 640 nm, immobilized in the film, which is carried out using a calibration curve with a sensitivity coefficient (5.6 ± 0.6) × 10 ⁴ and the correlation coefficient R ² = 0.99. The value of the limit of determination of sulfate ions was estimated as 1 × 10 ^-5 M. The disadvantage of the considered sensitive element is the heterogeneity of the optical and chemical properties of the gelatin film and their change in time, as well as a noticeable leaching of the reagent and insufficient adhesion of the gelatin film to the glass.

In patent RU 2067297 (IPC G01N 31/22, G01N 27/416, publ. 09/27/1996) disclosed indicator sensitive material (HMI) for rapid testing of traces of substances, including a solid carrier and an organic reagent, characterized in that as a solid polymeric fibrous materials with elementary units of cellulose or polyamide are used in the carrier, a reagent with high selectivity with respect to the ion being determined and the ability to strongly immobilize is used as an organic reagent, the material additionally contains optical rejection livatel belofor the following component ratio, May .: medium - 91-96% organic reagent - 3-10% belofor - 1-2%. Arsenazo III, arsenazo I, beryllon II, dithizone, blue-and-black eriochrom, α, α′-dipyridyl are preferably used as the organic reagent. The disadvantage of this opaque HMI is the use of diffuse reflection spectroscopy to determine the substance of interest, which is currently not very common in analytical laboratories, as well as the narrow range of acidity of the use of these HMI (from pH 1 and above). In acidic environments, these HMIs lose their chemical and mechanical strength and decompose.

Patent RU 2272284 (IPC G01N 31/22, published March 20, 2006) discloses an indicator sensitive material for determining the trace amounts of substances in the analyzed objects, including a solid polymer carrier with a reagent, characterized in that the solid polymer carrier is made of a transparent polymer material containing functional groups providing intermolecular interactions with a reagent and / or a defined substance and selected from the series: amide —CONH ₂ , carboxylic —COOH, hydroxyl —OH, nitrile —CN, ester —COO—, carbonyl > С = O, ether -O-, sulfur-containing -SH, -S-, -S = C, -SO ₃ H, -SCN, halogen-containing -CF ₃ , -F, -Cl, -I, organic and inorganic salt-like (alkyl) metal acrylates, metal carboxylates. The main disadvantage of this HMI is the narrow working pH range (a medium close to neutral). The applicability of the material in acidic environments, as well as the constancy and reproducibility of its analytical characteristics, are not adequately confirmed in the description.

Thus, there is a need to overcome the disadvantages of the prior art, expand the arsenal of sensitive elements, improve their stability and analytical characteristics. Utility Model Disclosure

As a result of the studies, the authors of the utility model found that the disadvantages of the prior art can be overcome by creating a sensitive element (optical sensor), including a layer of a copolymer of methyl methacrylate, ethyl acrylate and a quaternary salt of dimethylaminoethyl methacrylate with a chromogenic organic reagent (OR) immobilized in the layer, whose absorption spectrum in the visible region changes when interacting with the ionic form of the metal.

The technical result of the utility model is to expand the arsenal of spectrophotometric analysis tools (optical sensors), as well as test systems with a visual indication of the analytical signal (color change) to determine the ionic forms of metals forming colored compounds with OR. In particular, the utility model provides completely reversible optical sensors, in particular, for determining the concentration of metal ions in liquid media, which have long-term chemical and mechanical stability in strongly acidic aqueous media, as well as high sensitivity at low concentrations of immobilized OR. The technical result is achieved by including OR in the porous network structure of the polymer gel, characterized by good permeability for the analyte (ionic forms of metals), the ability to concentrate the analyte, as well as a large contact surface of the analyte with the OR.

In one aspect, the utility model provides an optical sensor for determining the ionic forms of metals, including an optically transparent substrate and a layer of organic polymer with a chromogenic organic reagent immobilized in the layer, characterized in that the organic polymer is a copolymer of methyl methacrylate, ethyl acrylate and the quaternary salt of dimethylaminoethyl methacrylate, and the layer of organic polymer is bound to a transparent substrate by adhesion forces.

As a substrate, it is preferable to use glass plates, such as standard glass slides SP-7102, size 76.2 × 25.4 mm and a thickness of 1.0-1.2 mm. However, there are no particular limitations on the nature and thickness of the substrate material, provided that the substrate is transparent in the analytical wavelength range and the polymer layer and the PR immobilized in it have an adhesion force sufficient to hold this layer on the substrate for a given CE service life, calculated, for example, by the number of cycles of "determination-regeneration".

As the organic polymer is preferably used a copolymer of methyl methacrylate, ethyl acrylate and dimethylaminoethyl quaternary salt, commercially available as the Eudragit ^® RS and Eudragit ^® RL.

The term “chromogenic OR” in the context of a utility model means that the OR changes color in the presence of the ionic form of the metal being determined.

The expression "changes color" means that the color of the OR before interacting with the ionic form of the metal is noticeably different from the color of the OR after interacting with the ionic form of the metal, which is perceived subjectively as color sensation and can be characterized by objective research data. For example, in the electronic absorption spectrum in the visible region, changes in the characteristics of absorption bands are observed (shift of the absorption maximum, changes in the contour of the absorption band, appearance / disappearance of the absorption band). The color change can also be numerically described by the color coordinate values in the selected color model (CIE XYZ, LMS, RGB, CMYK, etc.).

Preferably, the color change is reversible. In the context of a utility model, the term “reversible color change” means that the absorption intensity at a wavelength near the maximum absorption does not decrease by more than 5%, preferably by more than 2%, over a given lifetime of SE, calculated, for example, by the number of cycles “ definition-regeneration. "

In accordance with a useful model, reagents belonging to various classes of organic compounds that are soluble in polar organic solvents such as ethanol, isopropanol, methylene chloride, dichloroethane, acetonitrile, acetone, dimethylformamide in amounts ensuring the absorption of light A by a sensitive element can be used as a chromogenic OP wavelength in the region of the maximum absorption of OP in the presence of an ionic metal form in the range A from 0.05 to 2.0, preferably from 0.1 to 1.5. Depending on the molar mass (g / mol) and the molecular absorption index of the reaction product of OR with the ionic form of the metal (l / mol × cm), the solubility of OR in a polar organic solvent should be 1-10 mg / ml. For example, dimethylglyoxime or dithizone may be used for utility model purposes. Preferably, chromogenic ORs containing a dissociating anionic group or several identical or different dissociating anionic groups are used, which allows more reliable immobilization of OR in the copolymer layer due to ionic bonding of the OR anion group with the cationic center of the quaternary salt of dimethylaminoethyl methacrylate. Non-limiting examples of preferred chromogenic OPs are alizarin red, magnesia IREA, ferrosine, nitroso-R salt containing a sulfonyl (-SO ₃ H) group, arsenazo III containing sulfonyl (-SO ₃ H) and arsenyl (-AsO ₃ H ₂ ) groups, arsazene containing arsonyl (-AsO ₃ H ₂ ) groups. Also, chromogenic ORs may contain phosphonyl (—PO ₃ H ₂ ) or carboxyl (—COOH) groups.

Another aspect of the utility model is a method for manufacturing an optical sensor for determining the ionic forms of metals, comprising an optically transparent substrate and a layer of organic polymer with a chromogenic organic reagent immobilized in the layer, where the organic polymer is a copolymer of methyl methacrylate, ethyl acrylate and dimethylaminoethyl methacrylate quaternary salt, characterized in that the organic the polymer is mixed with OR in a mass ratio that ensures the absorption of light A by a sensitive element ntom at a wavelength in the region of the maximum absorption of OP in the presence of an ionic metal form in the range A from 0.05 to 2.0, the resulting mixture is dissolved in a volume of polar organic solvent, which ensures complete dissolution of the mixture to obtain a solution, the solution is applied to an optically transparent substrate and the organic solvent is evaporated to obtain a layer of organic polymer with a chromogenic organic reagent (OR) immobilized in the layer, which is held on an optically transparent substrate by adhesion forces.

The sensitive element is made by uniformly applying a solution of a polymer containing immobilized OR in an organic solvent to a defatted glass substrate and then drying to remove the solvent. Ethanol, dichloroethane, acetonitrile, acetone and the like polar solvents can be used as a solvent.

Using the presented developments, various sensitive elements (SEs) can be proposed, including an optically transparent substrate with a firmly held polymer layer, in which selective ORs are absorbed, the absorption spectrum of which in the visible region changes upon interaction with ionic forms of the metal. The interaction is carried out by introducing the indicator zone into the test solution with the determined element for 5-20 minutes. Then the device is dried. For the quantitative determination of metal, optical instruments are used that are capable of measuring absorption in the visible and UV regions, for example, a ThermoFisher Scientific Helios Z spectrophotometer (Great Britain) or visual recording by comparison with imitation color scales.

Thus, the combination of the mechanical and chemical properties of the polymer and the plate, as well as the analytical properties of the OR, allow concentrated determination of elements from solutions with any acidity, including strongly acidic solutions. CE, coated with a polymer layer containing OR, is immersed in the analyzed solution for 5-20 minutes (the exposure time is set depending on the nature of the OR used in CE and for each component determined separately). Then the plate is dried and the absorbance of the SE is measured.

Variants of using OR, the element being determined, and also the reaction conditions are given in the table.

Table. Sensor Features Organic reagent Detectable metal Color change of the sensor Reaction Conditions Arsenazo III Zr Pink green pH 0 U (VI); Th; REE (for REE pH 3) Ca ²⁺ Purple blue pH 11 Arsazen Cd Yellow red 2.5-4 Dithizon Ag Yellow violet pH 0 Cu (II) pH 1 Green yellow brown Dimethylglyoxime Re (IV) B / c - red 4% PO H ₂ SO ₄ Nitroso-R salt Fe (II) Yellow green 5-8 Ferrosin Fe (II) B / c - purple 4-8 Magneson IREA Mg (II) Blue pink 9-11 Alizarin red Al (III) Yellow red 4-6

Manufacturing methods

Method 1. Preparation of the indicator polymer zone of the CE: In penicillin vials, 10% alcohol solutions of polymers are prepared: 2 ml of C ₂ H ₅ OH + 0.184 g of Eudragit ^® polymer (RS or RL brand). They add the necessary sample of OR, tightly close the bottles with stoppers and achieve complete dissolution of the OR with stirring on a magnetic stirrer. Ethanol, dichloroethane, acetonitrile, acetone and the like can be used as solvents. Next, the dispenser is carefully applied to 0.1 ml of the obtained polymer solution with OR (table), previously washed in alcohol and dried, in a clean glass plate and distributed over a surface of 1 cm ² (using a stencil). It is dried in air at room temperature in a place protected from dust until a dense film is formed.

Method 2. Preparation of the indicator polymer zone, as in example 1, characterized in that the polymer solution is applied by pouring.

Method 3. Preparation of the indicator polymer zone, as in example 1, characterized in that the polymer solution is evenly distributed over the indicator zone of the glass matrix in a centrifugal field.

Method 4. Preparation of the indicator polymer zone, as in example 1, characterized in that the polymer solution, including the OP, is applied in a multilayer manner. Each subsequent layer is applied after the previous one has dried.

Brief description of drawings (formulas and diagrams)

FIGURE 1 is a formula of a single unit of a copolymer of methyl methacrylate, ethyl acrylate and a quaternary salt of dimethylaminoethyl methacrylate, in the ratio of monomer groups x = 0.3125; y = 0.625; z = 0.0625 (Eudragit ^® RL)

FIG.2 is a section of a sensitive element (SE). 1 - sensitive layer (Polymers Eudragit ^® RL or Eudragit ^® RS + OP), 2 - substrate (glass).

FIGURE 3 represents the dependence of the change in the absorption of the sensitive element on the iron ions (II) (OR - ferrosine) at λ = 563 nm in time.

FIGURE 4 is an absorption spectrum of the initial SE for iron (II) ions (OR-ferrosin) in the visible region.

5 is an absorption spectrum of a complex of iron (II) with ferrosin. 1 - in solution with _{Fe (II)} = 10 ^-4 M, with _fer = 0.2 μmol / ml; 2 - in CE with _{Fe (II)} = 10 ^-4 M, with _fer = 0.2 μmol / CE.

FIG.6 represents the absorption spectra of ChE on calcium (OR - arsenazo III). 1 - in the absence of calcium in the solution (absorption spectrum of OR in ChE; 2 - absorption spectrum of the complex of OR with calcium with _{Ca (II)} = 0.01 M, C _ars . _III = 0.5 μmol / ChE.

FIG.7 is the absorption spectra of the SE on copper (OR - dithizone). 1 - in the absence of copper in solution (absorption spectrum of OR in CE). 2 - absorption spectrum of the OR complex with copper with _{Cu (II)} = 0.02 μM, with _dit = 5.6 μmol / ChE.

Utility Model Implementation

Example 1

Ferrozine-based film concentrator and optical sensor for iron (II)

The preparation of SE is carried out according to method 1. For this, 2 mg of OR ferrosin and 2 ml of C ₂ H ₅ OH are added to the penicillin vial. After dissolution OR in alcohol there is introduced 0.184 g of Eudragit ^® RL polymer powder, closed tightly stoppered vial and achieve complete dissolution of the polymer in reagent alcohol solution under agitation on a magnetic stirrer (200 rev / min) (after at least 3 hours). Next, the dispenser is gently applied to 0.1 ml of the obtained polymer solution with OR pre-washed in alcohol and dried up in a clean glass plate and distributed over a surface of 2 cm ² (using a stencil). CE are dried in air at room temperature in a place protected from dust until a dense film is formed. The CE thus prepared is a colorless transparent polymer film tightly held on glass by adhesion forces (FIG. 2). The film practically does not swell in aqueous solutions (pH from 0 to 14) and is retained on glass placed in water for a long period (1 month). When the prepared ChE is immersed in a solution containing Fe (II) ions, the color of the ChE rapidly changes from colorless to violet. Coloring increases within 5 minutes. For quantitative measurements, the SE is immersed in 25 ml of a solution prepared from a solution of a sample containing Fe (II) ions. The test solution is prepared in a 25 ml volumetric flask. For this, an aliquot of the solution containing iron (sample or standard solution), 5 ml of a 10% aqueous solution of hydroxylamine (NH ₂ OH) and 5 ml of buffer solution (pH 4 to 8) are added to the flask and the volume in the flask is adjusted to tags with distilled water.

The test solution thus prepared from the volumetric flask is poured into a chemical cup of such a volume that the SE can be completely immersed in the solution. Before starting spectrophotometric measurements, the optical “0” of the device is set according to dry SE. After that, having fixed the SE in the paw on a tripod, and setting the stirring speed of the test solution on the magnetic stirrer 200 rpm, completely immerse the CE in the test solution for a certain time (5 minutes, Fig. 3). The color of the SE changes from colorless to violet (the absorption spectrum of the initial SE in UV and visible is shown in Fig. 4). Next, the absorption of the SE is measured at a wavelength corresponding to the maximum of the complex of ferrosin with iron (563 nm, Fig. 5) using a spectrophotometer. The concentration of iron (II) ions is defined as the average absorption measured by SE. The range of detectable concentrations is 0.1-6 mg Fe / L. It is also possible to use SE for the test of determination of Fe (II) with visual or colorimetric indication of the color signal.

Example 2

Hub film and optical sensor for metal ions using calcium (II) based on arsenazo III as an example

The preparation of Che is carried out according to method 1. The composition of the polymer solution used for the preparation of ChE based on arsenazo III; 40 mg AR arsenazo III is dissolved in 5 ml of C ₂ H ₅ OH. After dissolution OR in alcohol there is introduced 0.450 g of powder polymer Eudragit ^® RS. The sequence of procedures in the manufacture of CE corresponds to that described in example 1. The manufactured CE is a pink-purple transparent polymer film tightly held on glass by adhesion forces (Fig. 2). The maximum absorption of AR of arsenazo III in CE corresponds to 540 nm. The absorption spectrum of the reagent is shown in Fig.6 (1). When the prepared ChE is immersed in a solution containing calcium (II) ions, the color of the ChE rapidly changes from pink to blue, the maximum absorption of the complex in the ChE corresponds to 640 nm (Fig. 6 (2)). Coloring increases within 10 minutes. For quantitative measurements, the SE is immersed in 50 ml of a solution with an acidity of pH 7-11. Before starting spectrophotometric measurements, the optical “0” of the device is set according to dry SE. Next, the absorption of the SE is measured at a wavelength corresponding to the maximum of the complex of arsenazo III with calcium (640 nm, Fig. 6 (2)) using a spectrophotometer. The concentration of calcium ions (II) is defined as the average absorption measured by SE using a calibration graph. The minimum detectable concentration is 0.8 μg / ml.

Using similar approaches, it is possible to use a film-concentrator and an optical sensor based on arsenazo III to indicate and quantify the ions of rare-earth elements.

Example 3

Hub film and optical sensor for metal ions based on dithizone based on the example of copper (II) ions

The preparation of CE is carried out according to method 1. The composition of the polymer solution used to prepare CE on the basis of dithizone: 20 mg of OR dithizone is dissolved in 5 ml of acetonitrile (CH ₃ CN). After dissolving the OR to the same make 0,450 grams of polymer Eudragit ^® RS. The sequence of procedures in the manufacture of CE corresponds to that described in example 1. The manufactured CE is a green transparent polymer film tightly held on glass by adhesion forces (FIG. 2). The absorption maxima of OR dithizone in CE correspond to 445 and 615 nm. The absorption spectrum of the reagent is shown in Fig.7 (2). When the prepared ChE is immersed in a solution containing copper (II) ions, the color of the ChE rapidly changes from green to yellow-brown, the maximum absorption of the complex in ChE corresponds to 510 nm (Fig. 7 (1)). Coloring increases within 10 minutes. For quantitative measurements, the SE is immersed in 50 ml of a solution with an acidity of pH 1. The spectrophotometric measurement procedure is similar to that described in Example 2. The minimum detectable concentration is 1 ng / ml. CE is reversible, the complex with copper is easily destroyed by washing in a solution of 0.1 n HCl for 5 minutes.

Claims (3)

1. An optical sensor for determining the ionic forms of metals, including an optically transparent substrate and a layer of organic polymer with a chromogenic organic reagent immobilized in the layer, characterized in that the organic polymer is a copolymer of methyl methacrylate, ethyl acrylate and the quaternary salt of dimethylaminoethyl methacrylate, and the layer of the organic polymer is bonded to a transparent the substrate by adhesion forces, the mass ratio of the organic polymer and the OR in the polymer layer is in the range from 200/1 to 50/1. 2. The optical sensor according to claim 1, characterized in that the optically transparent substrate is a glass plate with a thickness of 1.0-1.5 mm 3. The optical sensor according to claim 1 or 2, characterized in that the chromogenic organic reagent is selected from the group consisting of alizarin red, magneson IREA, ferrosin, nitroso-R-salt, arsenazo III and arsazene.

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