RU133721U1 - DEVICE FOR FIXING A BANDBAND MATERIAL ON A BURN SURFACE OF AN EXPERIMENTAL ANIMAL - Google Patents

Abstract

A device for fixing dressing material on the burn surface of an experimental animal is characterized in that it is made of transparent elastic thermoplastic in accordance with the size of the experimental animal in the form of a pattern 0.5-1.5 mm thick, in the projection of a burn wound, the pattern has multiple holes with a diameter of 5- 7 mm, in one of the side parts of the pattern a hole was made for one of the front paws of the laboratory animal, the front part of the pattern in the form of two ribbons 3-4 cm wide serves for fixing the pattern and in the sternoclavicular compound, and the rear part of the pattern in the form of two strips 3-4 cm in width is used to fix the pattern of the abdomen, the device is provided with two additional strips serving to fix the front and rear tapes pattern for the inner surface of the front paws of the animal.

Description

The utility model relates to the field of experimental medicine and is intended to create an experimental model of burn injury in laboratory animals in order to optimally select etiotropic and pathogenetic drugs.

Significant material costs for the treatment of burn injuries, an increase in the number of burns with a severe and extremely severe degree of damage to the body, which often result in death or disability, require the development of effective and cheap methods of treating thermal injuries. The relevance of this problem is due to an increase in the number of thermal injuries among the population, and especially among children. According to WHO, thermal damage to the population is third among all other injuries. So, in the European Region in 2004, 1,700 children under the age of 19 died from burns [Report on the prevention of childhood injuries in Europe // World Health Organization. - http://www.euro.who.int/_data/assets/pdf_file/0005/98744/E92049R.pdf. Electronic resource. - 2009.]. Therefore, the use of experimental laboratory animals seems most appropriate for this purpose. However, laboratory animals tear the dressing from the burn surface, violating the sterility and effect of the drug.

A device for fixing an experimental animal is known [Patent RU No. 72405 of July 24, 2007] through the use of caudal, cranial and lateral limb fixators. The main disadvantage of the proposed utility model is the short time spent by the experimental animal in the fixing device, because feeding and excrement from the animal is not provided.

A device for reproducing a thermal burn on laboratory animals is proposed [Patent RU No. 91518 of 06.22.2009]. The utility model does not provide for the maintenance of a laboratory animal after applying a thermal burn.

Gutkin A.V. et al. proposed a safety chamber for experimental research of a burn wound in rats [Patent BY No. 7926 of 02.28.2012, publ. // Afitsyiny bul. / Nat. central intellectual. Ulasnasti. - 2012. - No 1. - S.256-257.]. A semitransparent plastic cylinder closed on one side with a plug is attached using suture material to the skin surface of the rat. The disadvantages of the proposed utility model: injury to the skin of the animal with suture material, when performing burn wound dressings, it is necessary to re-fix the safety chamber with suture material on the skin surface of the animal, insufficient oxygen supply under the safety chamber can contribute to the development of concomitant burn injury of anaerobic infection.

A device is known for fixing dressing material on laboratory animals in an experiment [Patent RU No. 107934 of 03/21/2011], selected by us as a prototype. The utility model consists of a support and fixing elements. The supporting element is made in the form of a plate with a graduated opening, and the fixing element is made in the form of holes along the perimeter of the plate for passage of the fixing ligatures through the skin of the laboratory animal (Fig. 1). The disadvantages of the proposed utility model: the burn surface may extend beyond the end of the opening and there is the difficulty of studying the effect of extensive burn injury on the body of the animal; moreover, with extensive burns, the plate does not extend beyond the burn wound and therefore it cannot be fixed on the burn surface. The imposition of fixing ligatures has a traumatic effect on the laboratory animal. A closed window opening restricts air oxygen to the surface of the burn wound, which creates favorable conditions for the development of anaerobic infection under the chemically inert material of the plate. Gangrene of a burn wound is not excluded.

The present invention is to develop a device for fixing dressings on the burn surface of experimental animals in conditions as close as possible to the treatment of patients with burn injuries.

The technical result is achieved by the fact that for the manufacture of the device used transparent elastic material, the permeability of which is ensured by the holes above the surface of the burn, and its fixation is carried out on the chest and abdominal surfaces of the animal.

The technical result is achieved in that the device for fixing the dressing on the burn surface of an experimental animal according to the invention is characterized in that it is made of transparent elastic thermoplastic in accordance with the size of the experimental animal in the form of a pattern 0.5-1.5 mm thick, in the projection of the burn wounds, the pattern has multiple holes with a diameter of 5-7 mm, in one of the side parts of the pattern a hole is made for one of the front paws of the laboratory animal, the front part, patterns in the form of two ribbons with a width of 3-4 cm serves to fix the pattern in the sternum-clavicular region, and the back of the pattern in the form of two ribbons 3-4 cm wide serves to fix the pattern in the abdomen, the device is equipped with two additional tapes serving for fixing front and rear ribbons of the pattern behind the inner surface of the front legs of the animal.

The proposed utility model is illustrated by graphic material. The figure 2 presents a General view of the device, where 1 is a pattern of thermoplastic for a laboratory animal, 2 are multiple holes with a diameter of 5-7 mm in the projection of a burn wound, 3 is the front of the pattern, 4 are tape patterns for attaching the device to the projection of the sternum clavicular joints, 5 - hole for one of the front legs of the animal, 6 - tape for fixing the pattern in the abdomen, 7 - the back of the pattern.

The figure 3 presents a pattern for a laboratory animal, where 1 is a pattern of thermoplastic, 2 are multiple holes with a diameter of 5-7 mm in the projection of a burn wound, 3 is the front of the pattern, 7 is the back of the pattern, 4 is the pattern tape for attaching the device in the projection of the sternoclavicular joints, 6 - two tapes for fixing the pattern in the abdomen, 5 - an opening for one of the front legs of the animal, 8 and 10 - two additional tapes serving to fix the front and rear tapes of the pattern behind the inner surface of the front legs of the animal, 9 - places of fastening of two additional tapes (8 and 10) with front and rear tapes of the pattern in the projection behind the inner surface of the front legs of the animal.

The proposed device is used as follows.

Polypropylene is used as a transparent elastic thermoplastic plastic - it is a solid thermoplastic polymer with high strength, elasticity, resistance to chemicals (acids, alkalis, salt solutions), transparency, physiological harmlessness and low cost [http://torelast.com.ua /info/index.htmr). Plastic manufacturer: PJSC “Slavic Plant Torelast” (84112, Ukraine, Donetsk region, Slavyansk, Podzodeznaya St., 3).

The area of thermoplastics was selected depending on the size of the experimental animal. The device is made in the form of a pattern of thermoplastic with a thickness of 0.5-1.5 mm (figure 3), in the projection of a burn wound, the pattern has multiple holes (2) with a diameter of 5-7 mm. In the front of the pattern there are two ribbons (4) 3-4 cm wide for fixing the pattern in the projection of the sternoclavicular joints, and in the back of the pattern there are two ribbons (6) 3-4 cm wide for fixing the pattern in the abdomen. In one of the side parts of the pattern, a hole is made for one of the front legs of the animal (5).

Under anesthesia, a laboratory animal was burned on the back, a bandage with the test drug was applied to the burned surface. and in the hole 5 of the pattern was placed the front right paw of the rabbit. The pattern was formed on the body of the rabbit and the staples for the stapler were fastened with ribbons of the front part of the pattern in the projection of the sternoclavicular joints (4), and the ribbons of the back of the pattern (6) were fastened with a stapler in the abdomen; and 6 patterns on the inside of the left and right front paws of the laboratory animal, providing additional fixation of the device. During repeated dressings, the staples were removed with nippers and another bandage was applied. The pattern was treated with disinfectant and. reused.

The proposed device allows for clinical observation of the animal, to record body temperature, pulse, respiratory rate and conduct laboratory tests during the entire period of observation of the treatment of burn wounds.

The proposed device is easily removed and put on when performing dressings on the burn surface, does not injure the animal, has low weight and does not limit mobility, which allows animals to be kept in ordinary cages.

An example of the practical use of the device:

Rabbits of the breed "chinchilla" to adapt to a new cage in accordance with the requirements of the sanitary rules (Approved by the Chief State Sanitary Doctor No. 1045-73) were quarantined for 3 days in a cage for an experiment. The rabbit was kept in a vivarium in accordance with the order of the Ministry of Health of the Russian Federation No. 267 of 06/19/2003 and the requirements of the European Convention (Strasbourg, 1986) on keeping, feeding and caring for experimental animals, removing them from the experiment and subsequent disposal.

Anesthesia was carried out according to the following method: premedication was carried out with a 2% solution of xylazine hydrochloride (rometar) at a dose of 5.0 mg / kg, and against its background, a 5% solution of zoletin was administered intramuscularly at a dose of 7.5 mg / kg of rabbit body weight, respectively. Hair was removed from the back and side of the rabbit's body. Then the surface cleared of wool was immersed in a water bath for 10 sec. at a temperature of 90 ° C. A sterile aseptic gauze dressing with levomekol ointment was applied to the burn surface.

Then, in accordance with the size of the rabbit, a pattern with multiple holes with a diameter of 5-7 mm in the projection of the applied burn surface was prepared from a temoplastic sheet with a thickness of 0.5-1.5 mm, 2 strips in the form of ribbons (4) 4 cm wide were cut from the front of the pattern to fix the pattern in the projection of the sternoclavicular joints, and 2 strips in the form of ribbons (6) 4 cm wide were cut from the back of the pattern to fix the pattern in the abdomen. In the right side surface of the pattern, a hole (5) was prepared for the right front paw of the animal.

Through the hole 5 in the pattern, the front right paw of the rabbit was introduced and a pattern was formed over the body of the animal. Brackets for the stapler secured the front ribbons of the pattern (4) in the projection of the sternoclavicular joints, and the rear ribbons of the pattern (6) in the abdomen. Additional tapes 8 and 10 connected tapes 4 and 6 behind the inner surfaces of the right and left front paws of the animal and fixed them with tapes 4 and 6 using staples for staples. Tapes 8 and 10 provided additional fixation of the device on the animal. In repeated dressings, the staples were removed with pliers and another bandage was applied. The pattern was treated with disinfectant and reused.

The developed technical solution of the utility model made it possible to conduct clinical observations of the animal, record the body temperature of the animal, pulse, respiration rate and conduct sampling for laboratory research during the entire period of treatment of a burn disease. The transparency of the polypropylene film made it possible to visually assess the state of the wound surface ..

The proposed device with a low cost did not injure the laboratory animal, easily removed and put on, did not reduce the rabbit's mobility, provided free access of oxygen to the surface of the burn wound, and underwent disinfection during repeated use. The small dimensions of the device and light weight made it possible to keep experimental animals in ordinary rabbit cages.

The device was introduced on the basis of LLC Scientific and Production Innovation Enterprise Tyumen Institute of Microbiological Technologies. 5 experiments were conducted on rabbits of the breed "chinchilla" weighing 1900-2500 gr. The device well fixed the dressing on the surface of the burn wound, the animals did not remove the dressing and led an active lifestyle in ordinary cages for keeping laboratory animals. The table gives a description of the proposed utility model in comparison with the prototype.

Table Distinctive features of the proposed device in comparison with the prototype Signs Prototype Proposed device Characteristics of the burn model. Contact burn (using a plate). Contact, hot liquids (water, etc.) and chemical solutions. Characteristics of the burn surface when using the device A small burn surface area limited by the size of the fenestrated opening and the door. The ability to simulate a burn surface of various sizes. Used material for the device A dense opaque plate that allows you to control the condition of the burn surface through the door. Transparent elastic plastic - polypropylene. Complications when fixing the device to an animal. Injury and the possibility of suppuration of the skin surface in the area of fixing ligatures. There are no complications when fixing the device. Duration of wearing the device on an animal During an acute burn period. During an acute and prolonged period of a burn disease.

Claims (1)

A device for fixing dressing material on the burn surface of an experimental animal is characterized in that it is made of transparent elastic thermoplastic in accordance with the size of the experimental animal in the form of a pattern 0.5-1.5 mm thick, in the projection of a burn wound, the pattern has multiple holes with a diameter of 5- 7 mm, in one of the side parts of the pattern a hole is made for one of the front paws of the laboratory animal, the front part of the pattern in the form of two ribbons 3-4 cm wide serves for fixing the pattern and in the sternoclavicular compound, and the rear part of the pattern in the form of two strips 3-4 cm in width is used to fix the pattern of the abdomen, the device is provided with two additional strips serving to fix the front and rear tapes pattern for the inner surface of the front paws of the animal.

Images