RO85360B1 - Process for preparing alkaline phosphatase - Google Patents
Process for preparing alkaline phosphataseInfo
- Publication number
- RO85360B1 RO85360B1 RO110036A RO11003683A RO85360B1 RO 85360 B1 RO85360 B1 RO 85360B1 RO 110036 A RO110036 A RO 110036A RO 11003683 A RO11003683 A RO 11003683A RO 85360 B1 RO85360 B1 RO 85360B1
- Authority
- RO
- Romania
- Prior art keywords
- alkaline phosphatase
- gel
- chloride solution
- dialysed
- sodium chloride
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Inventia se refera la un procedeu de obtinere a fosfatazei alcaline, enzima utilizata în stabilirea diagnosticului în cazul bolilor cu etiologie virala, bacteriana si parazitara cat si pentru identificarea si determinarea cantitativa a o serie de subsatante active biologic. Precipitatul obtinut de la purificarea fractiunilor II si III Chon din procedeul de obtinere a gamaglobulinei se dizolva în apa distilata se trateaza cu alcool butilic în proportie de 10% dupa îndepartarea proteinelor de balast fosfataza alcalina este precipitata cu sulfat de amoniu dupa care se îndeparteaza omauritatile prin absorbtie pe gel de hidroxid de aluminiu 2% în raport de 10 ml gel la 100 ml solutie ce contine fosfataza alcalina pe gel de aluminiu ce se adauga în proportie de 25 ml gel la 100 ml solutie proteica, dupa centrifugare se elueaza cu solutie de clorura de sodiu IM, solutie clorura de sodiu 2M, solutie clorura de sodiu 2M ce contine 2,5% acid glutamic, fosfataza alcalina desorbitade ultimile doua solutii se dializeaza în apa, se concentreaza în vid la o concentratie proteuca de 10mg/ml, se dializeaza apoi în solutie tampon fosfat alcalin, se filtreaza steril si se conditioneaza prin liofilizare sau stabilizare cu sulfat de amoniu sau glicerina.The invention relates to a process for obtaining alkaline phosphatase, the enzyme used in the diagnosis of diseases with viral, bacterial and parasitic etiology, and for the identification and quantitative determination of a series of biologically active sub-active substances. The precipitate obtained from the purification of the II and III Chun fractions from the gamaglobulin production process is dissolved in the distilled water treated with 10% butyl alcohol after the removal of the ballast proteins alkaline phosphatase is precipitated with ammonium sulphate after which the omaures are removed by absorption on a 2% aluminum hydroxide gel over 10 ml of gel per 100 ml solution containing alkaline phosphatase on aluminum gel, which is added in a proportion of 25 ml of gel to 100 ml of protein solution, after centrifugation eluted with a chloride solution 2M sodium chloride solution, 2M sodium chloride solution containing 2.5% glutamic acid, desorbent alkaline phosphatase, the latter two solutions are dialysed in water, concentrated in vacuo at a protective concentration of 10mg / ml, dialysed then in alkaline phosphate buffer, filter sterile and condition by lyophilization or stabilization with ammonium sulfate or glycerol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO83110036A RO85360A2 (en) | 1983-02-17 | 1983-02-17 | PROCESS FOR OBTAINING ALKALINE PHOSPHATASE PROCESS FOR OBTAINING PHOSPHATASE |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO83110036A RO85360A2 (en) | 1983-02-17 | 1983-02-17 | PROCESS FOR OBTAINING ALKALINE PHOSPHATASE PROCESS FOR OBTAINING PHOSPHATASE |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RO85360B1 true RO85360B1 (en) | 1984-11-30 |
| RO85360A2 RO85360A2 (en) | 1985-01-03 |
Family
ID=20112692
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RO83110036A RO85360A2 (en) | 1983-02-17 | 1983-02-17 | PROCESS FOR OBTAINING ALKALINE PHOSPHATASE PROCESS FOR OBTAINING PHOSPHATASE |
Country Status (1)
| Country | Link |
|---|---|
| RO (1) | RO85360A2 (en) |
-
1983
- 1983-02-17 RO RO83110036A patent/RO85360A2/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| RO85360A2 (en) | 1985-01-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lazarus et al. | Degradation of collagen by a human granulocyte collagenolytic system | |
| GB1441751A (en) | Purification of albumin by thermocoagulation | |
| EP0077870A2 (en) | Process for heat treatment of aqueous solution containing human blood coagulation factor VIII | |
| FR2457281A1 (en) | NOVEL 3-AMINOPROPANESULFONIC ACID DERIVATIVES WITH ENHANCED MEMBRANE ACTIVITY | |
| Burke | The purification of interferon | |
| BE901792A (en) | OPHTHALMIC SOLUTION AND ITS PREPARATION METHOD. | |
| Chaiet et al. | Isolation of a pure dextranase from Penicillium funiculosum | |
| Horwitz et al. | Peptide mapping by limited proteolysis in sodium dodecyl sulfate of the main intrinsic polypeptides isolated from human and bovine lens plasma membranes | |
| Fantl | A comparative study of blood coagulation in vertebrates | |
| Alais et al. | Isolation, purification, and analysis of two κ-casein-like fractions from sheep casein | |
| FR2432038A1 (en) | LABELS OF CHIMIOLUMINESCENT NAPHTHALENE-1,2-DICARBOXYLIC ACID HYDRAZIDE AND THEIR PREPARATION PROCESS | |
| Phatak | Fibrin producing factor in oral sub-mucous fibrosis | |
| RO85360B1 (en) | Process for preparing alkaline phosphatase | |
| Menkin | Studies on inflammation: Xvi. On the formation of a chemotactic substance by enzymatic action | |
| Nour-Eldin et al. | The blood clotting factors in human saliva | |
| FR2577048B1 (en) | REAGENT FOR THE HEMAGGLUTINATION DETERMINATION OF ANTIBODIES AGAINST BACTERIAL TOXINS, METHOD OF PREPARATION AND ITS APPLICATION | |
| ATE44750T1 (en) | PROCESS FOR PREPARING AN ANTI-HEMOPHILIA FACTOR CONCENTRATE. | |
| Koszalka et al. | The separation of two urinary desoxyribonucleases | |
| Myrvik et al. | Studies on antibacterial factors in mammalian tissues and fluids: II. Cofactor requirements of a serum bactericidin for Bacillus subtilis | |
| ATE88752T1 (en) | INACTIVATION OF HUMAN IMMUNODEFICIENCY VIRUS (HIV) IN PROTEIN SOLUTIONS BY PHENOLS. | |
| BG31214A3 (en) | Method for obtaining of human albumine | |
| Tauber | Synthesis of Protein-like Substances by Chymotrypsin from Dilute Peptic Digests and Their Electrophoretic Patterns | |
| Liao et al. | Isolation and characterization of glycoproteins from canine tracheal pouch secretions | |
| FR2425438A1 (en) | NEW ACID ((O- (DITHIOLANNE-1,3 IMINO-2) SUBSTITUTE) -2 ACETYL-AMINO) -7 CEPHALOSPORANIC DERIVATIVES, THEIR PROCESS FOR PREPARATION AND THEIR USE AS ANTIBACTERIAL MEDICINAL PRODUCTS | |
| RU2200354C2 (en) | Method for cleaning from liquid radioactive wastes |