RO83871B1 - Radioimunoanaliza pentru diagnosticul precoce al gestatiei - Google Patents
Radioimunoanaliza pentru diagnosticul precoce al gestatieiInfo
- Publication number
- RO83871B1 RO83871B1 RO110871A RO11087183A RO83871B1 RO 83871 B1 RO83871 B1 RO 83871B1 RO 110871 A RO110871 A RO 110871A RO 11087183 A RO11087183 A RO 11087183A RO 83871 B1 RO83871 B1 RO 83871B1
- Authority
- RO
- Romania
- Prior art keywords
- progesterone
- precocious
- immunoassay
- radio
- free serum
- Prior art date
Links
- 230000035935 pregnancy Effects 0.000 title abstract 2
- 238000003127 radioimmunoassay Methods 0.000 title abstract 2
- 238000003745 diagnosis Methods 0.000 title 1
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 abstract 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 abstract 3
- 229960003387 progesterone Drugs 0.000 abstract 3
- 239000000186 progesterone Substances 0.000 abstract 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract 2
- 210000002966 serum Anatomy 0.000 abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 1
- 229960000583 acetic acid Drugs 0.000 abstract 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 1
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 abstract 1
- 238000013399 early diagnosis Methods 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 abstract 1
- 239000012362 glacial acetic acid Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000008267 milk Substances 0.000 abstract 1
- 210000004080 milk Anatomy 0.000 abstract 1
- 235000013336 milk Nutrition 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000008363 phosphate buffer Substances 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Inventia se refera la o metoda radioimunoanaliza pentru diagnosticul precoce al gestatiei, ce consta în faptul ca se extrage progesteronul din lapte într-un solvent constituit din alcool metilic absolut, alcool etilic absolut, apa distilata, acid acetic glacial si fluoresceina, dupa care progesteronul extras se amesteca cu un amestec format din tampon fosfat, ser de cal, lipsit de progesteron si anticorpi antiprogesteron la care se adauga apoi solutie de progesteron 125I, se mentine la temperatura camerei si apoi la 4...6 degree C, se precipita apoi cu solutie de sulfat de amoniu, se centrifugheaza si se citeste radioactivitatea precipitatului obtinut.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO110871A RO83871B1 (ro) | 1983-05-06 | 1983-05-06 | Radioimunoanaliza pentru diagnosticul precoce al gestatiei |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO110871A RO83871B1 (ro) | 1983-05-06 | 1983-05-06 | Radioimunoanaliza pentru diagnosticul precoce al gestatiei |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RO83871A2 RO83871A2 (ro) | 1984-04-12 |
| RO83871B1 true RO83871B1 (ro) | 1984-05-30 |
Family
ID=20113036
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RO110871A RO83871B1 (ro) | 1983-05-06 | 1983-05-06 | Radioimunoanaliza pentru diagnosticul precoce al gestatiei |
Country Status (1)
| Country | Link |
|---|---|
| RO (1) | RO83871B1 (ro) |
-
1983
- 1983-05-06 RO RO110871A patent/RO83871B1/ro unknown
Also Published As
| Publication number | Publication date |
|---|---|
| RO83871A2 (ro) | 1984-04-12 |
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