PT82429B - NEW MUTANTS OF HUMAN TISSUE PLASMIONOGENIC ACTIVATOR - Google Patents

NEW MUTANTS OF HUMAN TISSUE PLASMIONOGENIC ACTIVATOR

Info

Publication number
PT82429B
PT82429B PT82429A PT8242986A PT82429B PT 82429 B PT82429 B PT 82429B PT 82429 A PT82429 A PT 82429A PT 8242986 A PT8242986 A PT 8242986A PT 82429 B PT82429 B PT 82429B
Authority
PT
Portugal
Prior art keywords
vectors
human tissue
partic
new
contg
Prior art date
Application number
PT82429A
Other languages
Portuguese (pt)
Other versions
PT82429A (en
Inventor
Herbert Louis Heyneker
Gordon Allen Vehar
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of PT82429A publication Critical patent/PT82429A/en
Publication of PT82429B publication Critical patent/PT82429B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Diabetes (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Hematology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Human tissue plasminogen activator (t-PA) mutant (I) is produced by recombinant cells and is resistant to specific enzymatic cleavage. (I) is esp. in single-chain form, stabilised by site-directed mutagenesis at a 2-chain cleavage site. Partic. the matural Arg at position 275 is replaced by Gly or Glu, or Ila at position 276 is replaced. Also new are (1) DNA sequences coding for (I); (2) expression vectors able to express (I) and (3) microorganisms and cell cultures transformed with these vectors. A native t-PA gene, contg. the sequence to be mutated, is inserted into the phage M13mp8, and the single-strand form of the prod. annealed to a synthetic oligonucleotide, co-ng. 1 or more triplets coding for the amino acid(s) to be substd. forming a double-strand region. Expression vectors contg. the new DNA sequences can be expressed in E.coli or partic. cultures of Chinese hamster ovary (using vectors which include the SV40 origin of replication as promoter). -
PT82429A 1985-04-22 1986-04-21 NEW MUTANTS OF HUMAN TISSUE PLASMIONOGENIC ACTIVATOR PT82429B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US72546885A 1985-04-22 1985-04-22
US84669786A 1986-04-01 1986-04-01

Publications (2)

Publication Number Publication Date
PT82429A PT82429A (en) 1986-05-01
PT82429B true PT82429B (en) 1988-03-03

Family

ID=27111150

Family Applications (1)

Application Number Title Priority Date Filing Date
PT82429A PT82429B (en) 1985-04-22 1986-04-21 NEW MUTANTS OF HUMAN TISSUE PLASMIONOGENIC ACTIVATOR

Country Status (21)

Country Link
US (1) US5147643A (en)
EP (1) EP0199574B1 (en)
JP (2) JPH0740940B2 (en)
AT (1) ATE68825T1 (en)
AU (1) AU596356B2 (en)
CA (1) CA1341580C (en)
DD (1) DD258026A5 (en)
DE (2) DE3613390C2 (en)
DK (1) DK175380B1 (en)
ES (2) ES8802184A1 (en)
FI (1) FI98930C (en)
FR (1) FR2581652B1 (en)
GB (1) GB2173804B (en)
GR (1) GR861037B (en)
HU (1) HU206520B (en)
IE (1) IE58574B1 (en)
IL (1) IL78571A (en)
MY (1) MY102550A (en)
NO (1) NO175216C (en)
NZ (1) NZ215920A (en)
PT (1) PT82429B (en)

Families Citing this family (56)

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JPS58149871A (en) * 1982-02-27 1983-09-06 本田技研工業株式会社 Burglar-proof device for two and three-wheel barrow
JPS6087751A (en) * 1983-10-20 1985-05-17 House Food Ind Co Ltd Production of retort bean curd
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HUT41632A (en) * 1985-03-06 1987-05-28 Survival Technology Process for preparing agents for the enhancement of protein absorption
US5736134A (en) * 1985-04-22 1998-04-07 Genentech, In.C Tissue plasminogen activator variants
FR2581652B1 (en) * 1985-04-22 1989-12-22 Genentech Inc NEW MUTANTS OF TISSUE ACTIVATOR OF HUMAN PLASMINOGEN
US5756093A (en) * 1985-04-22 1998-05-26 Genentech, Inc. Tissue plasminogen activator variants
US4959318A (en) * 1985-06-27 1990-09-25 Zymogenetics, Inc. Expression of protein C
US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
DK623186A (en) * 1985-12-23 1987-08-26 Chiron Corp POLYPEPTIDE WITH TISSUE PLASMINOGEN ACTIVATOR EFFECT
DK43387A (en) * 1986-01-29 1987-07-30 Beecham Group Plc FIBRINOLYTIC ENZYM
WO1987004722A1 (en) * 1986-01-31 1987-08-13 Genetics Institute, Inc. Novel thrombolytic proteins
US5837518A (en) * 1986-01-31 1998-11-17 Genetics Institute, Inc. Thrombolytic proteins
US5258298A (en) * 1986-01-31 1993-11-02 Genetics Institute, Inc. Deletion and glycosylation mutant of human tissue plasminogen activator
US5071972A (en) * 1986-01-31 1991-12-10 Genetics Institute, Inc. DNA sequences encoding novel thrombolytic proteins
IE81073B1 (en) * 1986-03-18 2000-01-12 Genentech Inc Modified human tissue-type plasminogen activator and its preparation
US5589361A (en) * 1986-03-18 1996-12-31 Genentech, Inc. Human tissue-type plasminogen activator variant
PT84589B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
PT84588B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
US5169763A (en) * 1986-04-08 1992-12-08 Transgene S.A., Institut Pasteur Viral vector coding glycoprotein of HIV-1
GB8701811D0 (en) * 1987-01-28 1987-03-04 Beecham Group Plc Compounds
MY103358A (en) * 1987-04-15 1993-06-30 Novartis Ag Process for the production of protiens.
NO882226L (en) * 1987-05-22 1988-11-23 Zymogenetics Inc FIBRINOLYTIC PROTEINS.
US5200340A (en) * 1987-05-22 1993-04-06 Zymogenetics, Inc. Thrombin-activated tissue plasminogen activators
NO179874C (en) * 1987-06-04 1997-01-02 Zymogenetics Inc A method of producing a t-PA analog, DNA sequence encoding the t-PA analog, and an expression vector comprising the DNA sequence and host cell comprising this
ES2058180T3 (en) * 1987-06-04 1994-11-01 Zymogenetics Inc MUTANT T-PA WITH KRINGLE REPLACEMENT.
WO1989000197A1 (en) * 1987-06-30 1989-01-12 Genentech, Inc. Improved processes for the treatment of vascular disease
US5302390A (en) * 1987-07-01 1994-04-12 Beecham Group Plc Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment
IL87276A (en) * 1987-08-03 1995-07-31 Fujisawa Pharmaceutical Co Analog of tissue plasminogen activator comprising only the kringle 2 and protease domain dna encoding the same processes for the preparation thereof and pharmaceutical compositions containing the same
JP2708749B2 (en) * 1987-08-10 1998-02-04 エーザイ株式会社 Injectable composition containing modified tPA
US5244676A (en) * 1987-10-09 1993-09-14 Monsanto Company Modified tissue plasminogen activator with modified glycosylation site
US5037752A (en) * 1987-10-09 1991-08-06 Monsanto Company Modified tissue plasminogen activator substituted at cysteine-73 and lysine-277
US4963357A (en) * 1987-10-09 1990-10-16 Monsanto Company Tissue plasminogen activator modified by the substitution of Arg for Cys at position 73, methods and pharmaceutical compositions therefor
DE3851117T2 (en) * 1987-10-16 1995-03-30 Genencor Int Sequence-specific acidic E. coli protease.
DE3854195T2 (en) * 1987-10-29 1995-12-21 Yamanouchi Pharma Co Ltd POLYPEPTIDE COMPOUNDS.
US5504001A (en) * 1987-11-25 1996-04-02 Zymogenetics, Inc. Hybrid plasminogen activator
US5094953A (en) * 1988-03-21 1992-03-10 Genentech, Inc. Human tissue plasminogen activator variants
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US5714145A (en) * 1988-09-02 1998-02-03 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
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US5190756A (en) * 1989-12-01 1993-03-02 Genentech, Inc. Methods and materials for expression of human plasminogen variant
US5087572A (en) * 1989-12-01 1992-02-11 Genentech, Inc. Dna encoding human plasminogen modified at the cleavage site
DE3942142A1 (en) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh STABILIZATION OF GLYCOSYLATED T-PA
JP3696617B2 (en) * 1991-12-16 2005-09-21 ジェネンテク，インコーポレイテッド T-PA substitution mutants with improved fibrin specificity
CA2125403C (en) * 1991-12-16 2002-08-27 Herbert L. Heyneker Novel tissue plasminogen activator variants
AU664469B2 (en) * 1992-06-03 1995-11-16 Genentech Inc. Tissue plasminogen activator glycosylation variants with improved therapeutic properties
GB2298821A (en) * 1995-03-15 1996-09-18 Prestek Ltd A ribbon winding mechanism
EP0827751B1 (en) * 1996-09-06 2003-02-26 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Medicinal composition containing tissue plasminogen activator and nicotinamide
US6706504B1 (en) 1996-11-12 2004-03-16 The Scripps Research Institute Tissue type plasminogen activator (t-PA) variants: compositions and methods of use
CN1244894A (en) * 1996-11-12 2000-02-16 斯克里普斯研究所 Tissue type plasminogen activator (t-PA) variants: compositions and methods of use
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WO2002020802A1 (en) * 2000-09-04 2002-03-14 Hunan Royal Biotech A cell line expressing mutant human tissue-type plasminogen activator, constructing strategy and method of expressed protein preparation
US7556807B2 (en) 2001-02-20 2009-07-07 Kane Biotech, Inc. Signal peptides, nucleic acid molecules and methods for treatment of caries
US7597895B2 (en) * 2001-02-20 2009-10-06 The Governing Council Of The University Of Toronto Signal peptides, nucleic acid molecules and methods for treatment of caries

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GT198302091A (en) * 1982-05-05 1984-10-25 HUMAN TISSUE PLASMINOGEN ACTIVATOR A), B), C).
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
IL90047A (en) * 1982-10-19 1992-12-01 Cetus Oncology Corp Cysteine-depleted biologically active muteins other than interferon - '
US4753879A (en) * 1984-08-27 1988-06-28 Biogen N.V. Modified tissue plasminogen activators
EP0201153A3 (en) * 1985-02-09 1987-10-07 Beecham Group Plc Modified enzyme and process for its preparation
FR2581652B1 (en) * 1985-04-22 1989-12-22 Genentech Inc NEW MUTANTS OF TISSUE ACTIVATOR OF HUMAN PLASMINOGEN
DK43387A (en) * 1986-01-29 1987-07-30 Beecham Group Plc FIBRINOLYTIC ENZYM
WO1987004722A1 (en) * 1986-01-31 1987-08-13 Genetics Institute, Inc. Novel thrombolytic proteins

Also Published As

Publication number Publication date
DK181286A (en) 1986-10-23
NO175216C (en) 1994-09-14
ES557800A0 (en) 1990-03-01
GB2173804B (en) 1989-07-05
IE861055L (en) 1986-10-22
AU5641686A (en) 1986-10-30
FI861673A (en) 1986-10-23
FR2581652A1 (en) 1986-11-14
FI98930B (en) 1997-05-30
JP2529816B2 (en) 1996-09-04
ES8802184A1 (en) 1988-04-16
EP0199574B1 (en) 1991-10-23
AU596356B2 (en) 1990-05-03
ATE68825T1 (en) 1991-11-15
GB2173804A (en) 1986-10-22
HUT40703A (en) 1987-01-28
GR861037B (en) 1986-07-16
MY102550A (en) 1992-07-31
NZ215920A (en) 1989-07-27
NO861559L (en) 1986-10-23
FR2581652B1 (en) 1989-12-22
JPS6224A (en) 1987-01-06
DD258026A5 (en) 1988-07-06
DK181286D0 (en) 1986-04-21
IE58574B1 (en) 1993-10-06
EP0199574A2 (en) 1986-10-29
IL78571A0 (en) 1986-08-31
NO175216B (en) 1994-06-06
GB8609683D0 (en) 1986-05-29
ES554251A0 (en) 1988-04-16
PT82429A (en) 1986-05-01
JPH06277071A (en) 1994-10-04
FI861673A0 (en) 1986-04-21
JPH0740940B2 (en) 1995-05-10
FI98930C (en) 1997-09-10
DE3613390C2 (en) 2001-10-11
HU206520B (en) 1992-11-30
DK175380B1 (en) 2004-09-20
CA1341580C (en) 2008-09-30
US5147643A (en) 1992-09-15
IL78571A (en) 1991-06-30
DE3613390A1 (en) 1986-10-30
DE3682104D1 (en) 1991-11-28
ES9000011A1 (en) 1990-03-01
EP0199574A3 (en) 1987-08-05

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