PT3126523T - Deteção da metilação de adn utilizando reações de ligação e nuclease combinadas - Google Patents

Deteção da metilação de adn utilizando reações de ligação e nuclease combinadas

Info

Publication number
PT3126523T
PT3126523T PT157728346T PT15772834T PT3126523T PT 3126523 T PT3126523 T PT 3126523T PT 157728346 T PT157728346 T PT 157728346T PT 15772834 T PT15772834 T PT 15772834T PT 3126523 T PT3126523 T PT 3126523T
Authority
PT
Portugal
Prior art keywords
detection
dna methylation
ligation reactions
combined nuclease
nuclease
Prior art date
Application number
PT157728346T
Other languages
English (en)
Original Assignee
Univ Cornell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Cornell filed Critical Univ Cornell
Publication of PT3126523T publication Critical patent/PT3126523T/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PT157728346T 2014-04-01 2015-03-31 Deteção da metilação de adn utilizando reações de ligação e nuclease combinadas PT3126523T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201461973496P 2014-04-01 2014-04-01

Publications (1)

Publication Number Publication Date
PT3126523T true PT3126523T (pt) 2020-05-15

Family

ID=54241423

Family Applications (1)

Application Number Title Priority Date Filing Date
PT157728346T PT3126523T (pt) 2014-04-01 2015-03-31 Deteção da metilação de adn utilizando reações de ligação e nuclease combinadas

Country Status (5)

Country Link
US (1) US10221447B2 (pt)
EP (1) EP3126523B1 (pt)
ES (1) ES2807222T3 (pt)
PT (1) PT3126523T (pt)
WO (1) WO2015153571A2 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
CN112513265A (zh) * 2018-05-22 2021-03-16 博诚研究中心 用于人类癌症检测的修饰核酸的靶向富集和测序
CN110607369A (zh) * 2019-09-24 2019-12-24 广州奥百阕谱生物科技有限公司 Septin9基因甲基化状态检测试剂盒及其应用
EP4298244A1 (en) 2021-02-23 2024-01-03 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
GB9609441D0 (en) 1996-05-04 1996-07-10 Zeneca Ltd Process
CA2255774C (en) 1996-05-29 2008-03-18 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6506594B1 (en) 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
WO2001079548A2 (en) 2000-04-14 2001-10-25 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
US20050239089A1 (en) 2003-06-06 2005-10-27 Johnson Martin D Mobility cassettes
PT1633888E (pt) 2003-06-17 2009-04-22 Keygene Nv Meios e método para a detecção de sequências de nucleótidos alvo utilizando ensaios de ligação com pares de sondas de oligonucleótido melhoradas
WO2005010199A2 (en) 2003-07-16 2005-02-03 Geneohm Sciences, Inc. Invasive cleavage reaction with electrochemical readout
US7358048B2 (en) 2004-02-10 2008-04-15 Cornell Research Foundation, Inc. Method for detection of promoter methylation status
ATE399884T1 (de) 2004-03-24 2008-07-15 Applera Corp Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden
US20060234252A1 (en) 2004-07-02 2006-10-19 Applera Corporation Methods and kits for methylation detection
US20070092883A1 (en) * 2005-10-26 2007-04-26 De Luwe Hoek Octrooien B.V. Methylation specific multiplex ligation-dependent probe amplification (MS-MLPA)
EP2414541B8 (en) * 2009-03-31 2014-09-10 Centre Hospitalier Universitaire Vaudois Methylation ligation-dependent macroarray (mlm)
EP2534263B1 (en) 2010-02-09 2020-08-05 Unitaq Bio Methods and compositions for universal detection of nucleic acids
US8916344B2 (en) 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
CA2863257C (en) 2012-02-14 2021-12-14 Cornell University Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions
WO2016154337A2 (en) 2015-03-23 2016-09-29 The University Of North Carolina At Chapel Hill Method for identification and enumeration of nucleic acid sequences, expression, splice variant, translocation, copy, or dna methylation changes using combined nuclease, ligase, polymerase, terminal transferase, and sequencing reactions

Also Published As

Publication number Publication date
WO2015153571A2 (en) 2015-10-08
EP3126523A4 (en) 2018-02-28
ES2807222T3 (es) 2021-02-22
US10221447B2 (en) 2019-03-05
WO2015153571A3 (en) 2015-12-30
EP3126523A2 (en) 2017-02-08
US20170191113A1 (en) 2017-07-06
EP3126523B1 (en) 2020-02-19

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