PT2488633E - Método in vitro para obtenção de fibroblastos intrahepáticos infectados com o vírus da hepatite - Google Patents

Método in vitro para obtenção de fibroblastos intrahepáticos infectados com o vírus da hepatite Download PDF

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Publication number
PT2488633E
PT2488633E PT107742801T PT10774280T PT2488633E PT 2488633 E PT2488633 E PT 2488633E PT 107742801 T PT107742801 T PT 107742801T PT 10774280 T PT10774280 T PT 10774280T PT 2488633 E PT2488633 E PT 2488633E
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PT
Portugal
Prior art keywords
intrahepatic
hepatitis
virus
obtaining
vitro method
Prior art date
Application number
PT107742801T
Other languages
English (en)
Inventor
Yvon Calmus
Filomena Conti
Original Assignee
Univ Paris Descartes
Assist Publ Hopitaux De Paris
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Paris Descartes, Assist Publ Hopitaux De Paris filed Critical Univ Paris Descartes
Publication of PT2488633E publication Critical patent/PT2488633E/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656Adult fibroblasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/24011Flaviviridae
    • C12N2770/24211Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24251Methods of production or purification of viral material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02Recovery or purification

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PT107742801T 2009-10-16 2010-10-15 Método in vitro para obtenção de fibroblastos intrahepáticos infectados com o vírus da hepatite PT2488633E (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0904962A FR2951458B1 (fr) 2009-10-16 2009-10-16 Methode de production de fibroblastes intrahepatiques infectes par le virus de l'hepatite c

Publications (1)

Publication Number Publication Date
PT2488633E true PT2488633E (pt) 2014-01-13

Family

ID=42133719

Family Applications (1)

Application Number Title Priority Date Filing Date
PT107742801T PT2488633E (pt) 2009-10-16 2010-10-15 Método in vitro para obtenção de fibroblastos intrahepáticos infectados com o vírus da hepatite

Country Status (6)

Country Link
US (1) US20130101986A1 (pt)
EP (1) EP2488633B1 (pt)
ES (1) ES2437922T3 (pt)
FR (1) FR2951458B1 (pt)
PT (1) PT2488633E (pt)
WO (1) WO2011045772A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL2010294C2 (en) * 2013-02-13 2014-08-14 Boudewijn John Sambeek Connection device, assembly thereof and assembly method therefor.

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8017391B2 (en) * 2006-03-16 2011-09-13 Univesity of Florida Research Foundation, Inc. Human liver cancer cell line

Also Published As

Publication number Publication date
EP2488633A1 (fr) 2012-08-22
EP2488633B1 (fr) 2013-10-09
WO2011045772A1 (fr) 2011-04-21
FR2951458B1 (fr) 2011-12-16
ES2437922T3 (es) 2014-01-15
US20130101986A1 (en) 2013-04-25
FR2951458A1 (fr) 2011-04-22

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