PT117786A - PROTEIN CARRIER SYSTEM BASED ON NANO-SIZED CYANOBACTERIAL EXTRACELLULAR VESICLES - Google Patents

PROTEIN CARRIER SYSTEM BASED ON NANO-SIZED CYANOBACTERIAL EXTRACELLULAR VESICLES

Info

Publication number
PT117786A
PT117786A PT117786A PT11778622A PT117786A PT 117786 A PT117786 A PT 117786A PT 117786 A PT117786 A PT 117786A PT 11778622 A PT11778622 A PT 11778622A PT 117786 A PT117786 A PT 117786A
Authority
PT
Portugal
Prior art keywords
transformed
strain
synechocystis
current invention
protein
Prior art date
Application number
PT117786A
Other languages
Portuguese (pt)
Inventor
Miguel Nunes De Oliveira Paulo
Alexandre Ferreira Matinha Cardoso Jorge
Da Silva Lima Steeve
Alexandra Dos Reis Serra Cláudia
Fernandes Coutinho Filipe
Manuel Pereira De Oliva Teles Aires
Original Assignee
Univ Do Porto
Inst De Biologia Molecular E Celular Ibmc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Do Porto, Inst De Biologia Molecular E Celular Ibmc filed Critical Univ Do Porto
Priority to PT117786A priority Critical patent/PT117786A/en
Priority to PCT/PT2023/050003 priority patent/WO2023153949A1/en
Publication of PT117786A publication Critical patent/PT117786A/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/748—Cyanobacteria, i.e. blue-green bacteria or blue-green algae, e.g. spirulina
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/5005—Wall or coating material
    • A61K9/5063—Compounds of unknown constitution, e.g. material from plants or animals
    • A61K9/5068—Cell membranes or bacterial membranes enclosing drugs
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A INVENÇÃO ATUAL REFERE-SE A UMA CONSTRUÇÃO GENÉTICA PARA O TRANSPORTE DE UMA PROTEÍNA DE INTERESSE EM VESÍCULAS EXTRACELULARES (EV) DE SYNECHOCYSTIS SP. PCC6803. A INVENÇÃO ATUAL RELACIONA-SE AINDA COM A ESTIRPE SYNECHOCYSTIS TRANSFORMADA DERIVADA QUE COMPREENDE O REFERIDO SISTEMA. AS CIANOBACTÉRIAS TRANSFORMADOS COM O REFERIDO SISTEMA SÃO, SURPREENDENTEMENTE, CARACTERIZADOS POR HIPERVESICULAÇÃO DOS EVS EM MAIORES QUANTIDADES DO QUE AS ESTIRPES SELVAGENS E MUTANTES CONHECIDAS NO ESTADO DA ARTE. AS CIANOBACTÉRIAS TRANSFORMADAS COM A REFERIDA CONSTRUÇÃO SEGREGAM A PROTEÍNA DE INTERESSE EM EVS E PARA O MEIO EXTRACELULAR SENDO QUE ASSIM, A INVENÇÃO PRESENTE TAMBÉM SE REFERE A COMPOSIÇÕES COMPOSTAS POR EV DERIVADAS DA REFERIDA ESTIRPE SYNECHOCYSTIS TRANSFORMADA, BEM COMO COMPOSIÇÕES QUE COMPREENDEM O MEIO EXTRACELULAR SEM EV DERIVADO DA DITA ESTIRPE TRANSFORMADA DE SYNECHOCYSTIS, PARA UTILIZAÇÃO NO TRANSPORTE DE PROTEÍNAS/ENZIMAS EM ANIMAIS, INCLUINDO MAMÍFEROS E PEIXES. A INVENÇÃO ATUAL REFERE-SE AINDA AOS MÉTODOS DE TRANSFORMAÇÃO DE CÉLULAS EPITELIAS DE MAMÍFEROS COM EVS ISOLADAS DA REFERIDA ESTIRPE SYNECHOCYSTIS, QUE INTERIORIZAM AS REFERIDAS EV E APRESENTAM A PROTEÍNA DE INTERESSE. ALÉM DISSO, A INVENÇÃO ATUAL REFERE-SE A MÉTODOS PELOS QUAIS AS EV CIANOBACTERIANAS PODEM FUNCIONAR COMO NANOTRANSPORTADORES DE PROTEÍNAS INDIVIDUALIZADAS EM PEIXES, COM APLICAÇÕES PROSPETIVAS NA MODULAÇÃO DO ESTADO NUTRICIONAL OU ESTIMULANDO RESPOSTAS IMUNES ADAPTATIVAS ESPECÍFICAS. A CONSTRUÇÃO, ESTIRPE, COMPOSIÇÕES E MÉTODOS NA PRESENTE INVENÇÃO PODEM SER VANTAJOSAMENTE UTILIZADOS COMO UMA NOVA FERRAMENTA 2 BIOTECNOLÓGICA PARA A ENTREGA ESPECÍFICA DE PROTEÍNAS E ENZIMAS BIOLOGICAMENTE ATIVAS NA BIOTECNOLOGIA EM GERAL E NA AQUACULTURA EM ESPECÍFICO.THE CURRENT INVENTION REFERS TO A GENETIC CONSTRUCTION FOR THE TRANSPORT OF A PROTEIN OF INTEREST IN EXTRACELLULAR VESCLES (EV) OF SYNECHOCYSTIS SP. PCC6803. THE CURRENT INVENTION FURTHER RELATES TO THE DERIVED TRANSFORMED SYNECHOCYSTIS STRAIN THAT COMPRISES SAID SYSTEM. THE CYANOBACTERIA TRANSFORMED WITH THE SAID SYSTEM ARE, SURPRISINGLY, CHARACTERIZED BY HYPERVESICULATION OF EVS IN GREATER AMOUNTS THAN THE WILD AND MUTANTS STRAINS KNOWN IN THE STATE OF THE ART. CYANOBACTERIA TRANSFORMED WITH SAID CONSTRUCTION SECRETATE THE PROTEIN OF INTEREST INTO EVS AND INTO THE EXTRACELLULAR ENVIRONMENT THEREFORE, THE PRESENT INVENTION ALSO REFERS TO COMPOSITIONS COMPOSED BY EV DERIVED FROM SAID TRANSFORMED SYNECHOCYSTIS STRAIN, AS WELL AS COMPOSITIONS COMPRISING THE EXTRACELLULAR MEDIUM WITHOUT EV DERIVED FROM SAID TRANSFORMED STRAIN OF SYNECHOCYSTIS, FOR USE IN THE TRANSPORT OF PROTEINS/ENZYMES IN ANIMALS, INCLUDING MAMMALS AND FISH. THE CURRENT INVENTION FURTHER REFERS TO METHODS OF TRANSFORMING MAMMALIAN EPITHELIAS CELLS WITH EVS ISOLATED FROM SAID SYNECHOCYSTIS STRAIN, WHICH INTERIORIZE SAID EV AND PRESENT THE PROTEIN OF INTEREST. IN ADDITION, THE CURRENT INVENTION REFERS TO METHODS BY WHICH EV CYANOBACTERIALS CAN FUNCTION AS NANOTRANSPORTERS OF INDIVIDUALIZED PROTEINS IN FISH, WITH PROSPECTIVE APPLICATIONS IN MODULATING NUTRITIONAL STATUS OR STIMULATING SPECIFIC ADAPTIVE IMMUNE RESPONSES. THE CONSTRUCTION, STRAIN, COMPOSITIONS AND METHODS IN THE PRESENT INVENTION CAN BE ADVANTAGEOUSLY USED AS A NEW BIOTECHNOLOGICAL TOOL FOR THE SPECIFIC DELIVERY OF BIOLOGICALLY ACTIVE PROTEINS AND ENZYMES IN BIOTECHNOLOGY IN GENERAL AND IN AQUACULTURE IN SPECIFIC.

PT117786A 2022-02-10 2022-02-10 PROTEIN CARRIER SYSTEM BASED ON NANO-SIZED CYANOBACTERIAL EXTRACELLULAR VESICLES PT117786A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PT117786A PT117786A (en) 2022-02-10 2022-02-10 PROTEIN CARRIER SYSTEM BASED ON NANO-SIZED CYANOBACTERIAL EXTRACELLULAR VESICLES
PCT/PT2023/050003 WO2023153949A1 (en) 2022-02-10 2023-02-10 Protein carrier system based on cyanobacterial nano-sized extracellular vesicles

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PT117786A PT117786A (en) 2022-02-10 2022-02-10 PROTEIN CARRIER SYSTEM BASED ON NANO-SIZED CYANOBACTERIAL EXTRACELLULAR VESICLES

Publications (1)

Publication Number Publication Date
PT117786A true PT117786A (en) 2023-08-24

Family

ID=85283703

Family Applications (1)

Application Number Title Priority Date Filing Date
PT117786A PT117786A (en) 2022-02-10 2022-02-10 PROTEIN CARRIER SYSTEM BASED ON NANO-SIZED CYANOBACTERIAL EXTRACELLULAR VESICLES

Country Status (2)

Country Link
PT (1) PT117786A (en)
WO (1) WO2023153949A1 (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108703977B (en) * 2018-08-22 2019-07-02 中南大学湘雅医院 Application of cyanobacteria and membrane-outer vesicle thereof in preparation of medicine for treating diseases
EP3725371A1 (en) * 2019-04-18 2020-10-21 BiOMVis Srl Method for the production of outer membrane vesicles and immunogenic compositions thereof

Also Published As

Publication number Publication date
WO2023153949A1 (en) 2023-08-17

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Effective date: 20230821