PL447490A1 - Enzymatic system, electrode containing this system, method for manufacturing this electrode and its use for cholesterol determination, biosensor containing this electrode, and method for cholesterol determination - Google Patents

Enzymatic system, electrode containing this system, method for manufacturing this electrode and its use for cholesterol determination, biosensor containing this electrode, and method for cholesterol determination

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Publication number
PL447490A1
PL447490A1 PL447490A PL44749024A PL447490A1 PL 447490 A1 PL447490 A1 PL 447490A1 PL 447490 A PL447490 A PL 447490A PL 44749024 A PL44749024 A PL 44749024A PL 447490 A1 PL447490 A1 PL 447490A1
Authority
PL
Poland
Prior art keywords
electrode
cholesterol
application
biosensor
subject
Prior art date
Application number
PL447490A
Other languages
Polish (pl)
Inventor
Barbara KOWALEWSKA
Justyna MAKOWSKA
Original Assignee
Uniwersytet Warszawski
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Warszawski filed Critical Uniwersytet Warszawski
Priority to PL447490A priority Critical patent/PL447490A1/en
Priority to PCT/PL2025/050001 priority patent/WO2025151040A1/en
Publication of PL447490A1 publication Critical patent/PL447490A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia jest układ enzymatyczny zawierający nanostruktury węglowe zmodyfikowane polielektrolitem kationowym, przy czym na nanostrukturach węglowych zmodyfikowanych polielektrolitem kationowym unieruchomiona jest dehydrogenaza cholesterolowa. Przedmiotem zgłoszenia jest również elektroda zawierająca podłoże przewodzące, na którym umieszczony jest wspomniany powyżej układ enzymatyczny, a także sposób wytwarzania tej elektrody i jej zastosowanie do oznaczania cholesterolu. Ponadto, przedmiotem zgłoszenia jest zastosowanie powyżej wspomnianej elektrody jako jednej z elektrod pracujących w bioczujniku zawierającym więcej niż jedną elektrodę pracującą i przystosowanym do oznaczania więcej niż jednego analitu. Kolejnym przedmiotem zgłoszenia jest bioczujnik do oznaczania cholesterolu zawierający wspomnianą powyżej elektrodę jako elektrodę pracującą. Na koniec, przedmiotem zgłoszenia jest sposób oznaczania cholesterolu obejmujący kontaktowanie próbki zawierającej cholesterol ze wspomnianym powyżej bioczujnikiem w obecności kofaktora oraz elektrochemiczne oznaczanie cholesterolu w próbce.The subject of the application is an enzymatic system containing carbon nanostructures modified with a cationic polyelectrolyte, wherein cholesterol dehydrogenase is immobilized on the carbon nanostructures modified with the cationic polyelectrolyte. The subject of the application also includes an electrode containing a conductive substrate on which the aforementioned enzymatic system is placed, as well as a method for manufacturing this electrode and its use for determining cholesterol. Furthermore, the subject of the application is the use of the aforementioned electrode as one of the working electrodes in a biosensor containing more than one working electrode and adapted to determine more than one analyte. Another subject of the application is a biosensor for determining cholesterol containing the aforementioned electrode as the working electrode. Finally, the subject of the application is a method for determining cholesterol, comprising contacting a sample containing cholesterol with the aforementioned biosensor in the presence of a cofactor and electrochemically determining cholesterol in the sample.

PL447490A 2024-01-10 2024-01-10 Enzymatic system, electrode containing this system, method for manufacturing this electrode and its use for cholesterol determination, biosensor containing this electrode, and method for cholesterol determination PL447490A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL447490A PL447490A1 (en) 2024-01-10 2024-01-10 Enzymatic system, electrode containing this system, method for manufacturing this electrode and its use for cholesterol determination, biosensor containing this electrode, and method for cholesterol determination
PCT/PL2025/050001 WO2025151040A1 (en) 2024-01-10 2025-01-07 Electroenzymatic cholesterol biosensor, manufacture and use thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL447490A PL447490A1 (en) 2024-01-10 2024-01-10 Enzymatic system, electrode containing this system, method for manufacturing this electrode and its use for cholesterol determination, biosensor containing this electrode, and method for cholesterol determination

Publications (1)

Publication Number Publication Date
PL447490A1 true PL447490A1 (en) 2025-07-14

Family

ID=95065716

Family Applications (1)

Application Number Title Priority Date Filing Date
PL447490A PL447490A1 (en) 2024-01-10 2024-01-10 Enzymatic system, electrode containing this system, method for manufacturing this electrode and its use for cholesterol determination, biosensor containing this electrode, and method for cholesterol determination

Country Status (2)

Country Link
PL (1) PL447490A1 (en)
WO (1) WO2025151040A1 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0794429A1 (en) * 1996-03-07 1997-09-10 Matsushita Electric Industrial Co., Ltd. Cholesterol sensor
WO2020159314A1 (en) * 2019-01-31 2020-08-06 고려대학교 산학협력단 Enzyme-carrier complex

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4742650B2 (en) * 2005-04-08 2011-08-10 東レ株式会社 Carbon nanotube composition, biosensor, and production method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0794429A1 (en) * 1996-03-07 1997-09-10 Matsushita Electric Industrial Co., Ltd. Cholesterol sensor
WO2020159314A1 (en) * 2019-01-31 2020-08-06 고려대학교 산학협력단 Enzyme-carrier complex

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MELLISA DER BARRIO ET AL.: "Sensors and Actuators B: Chemical", "CARBON NANODOTS MODIFIED-ELECTRODE FOR PEROXIDE-FREE CHOLESTEROL BIOSENSING AND BIOFUEL CELL DESIGN" *

Also Published As

Publication number Publication date
WO2025151040A1 (en) 2025-07-17

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