PL447278A1 - Zastosowanie polimerazy TaqNeqSSB do normalizacji temperatury przyłączania sond i starterów w reakcji wykrywania jednonukleotydowych mutacji SNP - Google Patents

Zastosowanie polimerazy TaqNeqSSB do normalizacji temperatury przyłączania sond i starterów w reakcji wykrywania jednonukleotydowych mutacji SNP

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Publication number
PL447278A1
PL447278A1 PL447278A PL44727823A PL447278A1 PL 447278 A1 PL447278 A1 PL 447278A1 PL 447278 A PL447278 A PL 447278A PL 44727823 A PL44727823 A PL 44727823A PL 447278 A1 PL447278 A1 PL 447278A1
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PL
Poland
Prior art keywords
taqneqssb
polymerase
application
annealing temperature
detection reaction
Prior art date
Application number
PL447278A
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English (en)
Inventor
Kasjan SZEMIAKO
Marta SKWARECKA
Sabina ŻOŁĘDOWSKA
Dawid NIDZWORSKI
Original Assignee
Instytut Biotechnologii I Medycyny Molekularnej
Geneme Molecular Spółka Z Ograniczoną Odpowiedzialnością
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Application filed by Instytut Biotechnologii I Medycyny Molekularnej, Geneme Molecular Spółka Z Ograniczoną Odpowiedzialnością filed Critical Instytut Biotechnologii I Medycyny Molekularnej
Priority to PL447278A priority Critical patent/PL447278A1/pl
Priority to EP24223119.9A priority patent/EP4585698A3/en
Publication of PL447278A1 publication Critical patent/PL447278A1/pl

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem niniejszego zgłoszenia jest jedno z zastosowań polimerazy TaqNeqSSB w normalizacji temperatury przyłączania sond i starterów wielu niezależnych reakcji amplifikacji w celu ujednolicenia metody identyfikacji wielu jednonukleotydowych mutacji (SNP) oraz możliwości przeprowadzenia wielu niezależnych oznaczeń w jednym czasie i w jednakowym profilu temperaturowo-czasowym.
PL447278A 2023-12-23 2023-12-23 Zastosowanie polimerazy TaqNeqSSB do normalizacji temperatury przyłączania sond i starterów w reakcji wykrywania jednonukleotydowych mutacji SNP PL447278A1 (pl)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL447278A PL447278A1 (pl) 2023-12-23 2023-12-23 Zastosowanie polimerazy TaqNeqSSB do normalizacji temperatury przyłączania sond i starterów w reakcji wykrywania jednonukleotydowych mutacji SNP
EP24223119.9A EP4585698A3 (en) 2023-12-23 2024-12-23 Use of taqneqssb polymerase to normalize probe and primer annealing temperature in the single-nucleotide snp mutation detection reaction

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL447278A PL447278A1 (pl) 2023-12-23 2023-12-23 Zastosowanie polimerazy TaqNeqSSB do normalizacji temperatury przyłączania sond i starterów w reakcji wykrywania jednonukleotydowych mutacji SNP

Publications (1)

Publication Number Publication Date
PL447278A1 true PL447278A1 (pl) 2025-06-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PL447278A PL447278A1 (pl) 2023-12-23 2023-12-23 Zastosowanie polimerazy TaqNeqSSB do normalizacji temperatury przyłączania sond i starterów w reakcji wykrywania jednonukleotydowych mutacji SNP

Country Status (2)

Country Link
EP (1) EP4585698A3 (pl)
PL (1) PL447278A1 (pl)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007029200A2 (en) * 2005-09-09 2007-03-15 Invitrogen Corporation Ssb - polymerase fusion proteins

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL243940B1 (pl) * 2021-05-19 2023-11-06 Inst Biotechnologii I Medycyny Molekularnej Polimeraza Taq-NeqSSB, sposób jej otrzymywania, plazmid rekombinantowy, startery oraz zastosowanie polimerazy

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007029200A2 (en) * 2005-09-09 2007-03-15 Invitrogen Corporation Ssb - polymerase fusion proteins

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OLSZEWSKI M. ET AL.: "(2017) PLoS ONE 12(9): e0184162", FUSION OF TAQ DNA POLYMERASE WITH SINGLE-STRANDED DNA BINDING-LIKE PROTEIN OF NANOARCHAEUM EQUITANS - EXPRESSION AND CHARACTERIZATION *

Also Published As

Publication number Publication date
EP4585698A3 (en) 2025-09-17
EP4585698A2 (en) 2025-07-16

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