PL446262A1 - Aptamer, aptasensor containing aptamer and use of aptamer for selective detection of manganese ions - Google Patents

Aptamer, aptasensor containing aptamer and use of aptamer for selective detection of manganese ions

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Publication number
PL446262A1
PL446262A1 PL446262A PL44626223A PL446262A1 PL 446262 A1 PL446262 A1 PL 446262A1 PL 446262 A PL446262 A PL 446262A PL 44626223 A PL44626223 A PL 44626223A PL 446262 A1 PL446262 A1 PL 446262A1
Authority
PL
Poland
Prior art keywords
aptamer
manganese ions
aptasensor
solution
receptor layer
Prior art date
Application number
PL446262A
Other languages
Polish (pl)
Other versions
PL248352B1 (en
Inventor
Marta JARCZEWSKA
Elżbieta Malinowska
Marcin Olszewski
Original Assignee
Politechnika Warszawska
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Politechnika Warszawska filed Critical Politechnika Warszawska
Priority to PL446262A priority Critical patent/PL248352B1/en
Priority to PCT/PL2024/050054 priority patent/WO2025071421A1/en
Publication of PL446262A1 publication Critical patent/PL446262A1/en
Publication of PL248352B1 publication Critical patent/PL248352B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18—Water
    • G01N33/1813—Specific cations in water, e.g. heavy metals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/607—Detection means characterised by use of a special device being a sensor, e.g. electrode

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Optics & Photonics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia jest aptamer zdolny do specyficznego wykrywania jonów manganu, który ma sekwencję nukleotydową przedstawioną w SEQ.ID NO.1. Aptasensor mający powinowactwo do jonów manganu Mn2+, który zawiera złotą elektrodę roboczą (1) pokrytą warstwą receptorową (2) zawierającą aptamery (3) i cząsteczki wypełniacza (4) oraz znacznik anionowy (5) znajdujący się w roztworze. Sposób otrzymywania aptasensora obejmujący etap tworzenia warstwy receptorowej (2) przez inkubację złotych elektrod (1) w roztworze aptameru (3) przez 2h oraz etap b) inkubacji z roztworem wypełniacza (4) przez 1h w celu wyeliminowania niespecyficznych oddziaływań na powierzchni elektrody. Zastosowanie aptamerów jako warstwy receptorowej biosensora do selektywnego wykrywania jonów manganu in situ za pomocą technik elektrochemicznych.The subject of the application is an aptamer capable of specifically detecting manganese ions, which has the nucleotide sequence shown in SEQ.ID NO.1. An aptasensor having an affinity for manganese ions Mn2+, which comprises a gold working electrode (1) covered with a receptor layer (2) containing aptamers (3) and filler molecules (4) and an anionic label (5) contained in solution. A method for obtaining an aptasensor comprising the step of forming a receptor layer (2) by incubating gold electrodes (1) in an aptamer solution (3) for 2 hours and a step b) of incubating with a filler solution (4) for 1 hour to eliminate non-specific interactions on the electrode surface. Use of aptamers as a receptor layer of a biosensor for selective detection of manganese ions in situ by means of electrochemical techniques.

PL446262A 2023-09-29 2023-09-29 Aptamer, aptasensor containing aptamer and use of aptamer for selective detection of manganese ions PL248352B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL446262A PL248352B1 (en) 2023-09-29 2023-09-29 Aptamer, aptasensor containing aptamer and use of aptamer for selective detection of manganese ions
PCT/PL2024/050054 WO2025071421A1 (en) 2023-09-29 2024-07-29 Aptamer, aptasensor comprising aptamer, and use of the aptamer for selective detection of manganese ions

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL446262A PL248352B1 (en) 2023-09-29 2023-09-29 Aptamer, aptasensor containing aptamer and use of aptamer for selective detection of manganese ions

Publications (2)

Publication Number Publication Date
PL446262A1 true PL446262A1 (en) 2025-03-31
PL248352B1 PL248352B1 (en) 2025-12-01

Family

ID=92711097

Family Applications (1)

Application Number Title Priority Date Filing Date
PL446262A PL248352B1 (en) 2023-09-29 2023-09-29 Aptamer, aptasensor containing aptamer and use of aptamer for selective detection of manganese ions

Country Status (2)

Country Link
PL (1) PL248352B1 (en)
WO (1) WO2025071421A1 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10520462B1 (en) * 2018-11-24 2019-12-31 Alfaisal University Electrochemical screening for the selection of DNA aptamers

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111562298A (en) * 2020-05-20 2020-08-21 海南师范大学 Construction and application of an electrochemical aptamer sensor for highly sensitive detection of lead ions using methylene blue as an indicator
TWI767419B (en) * 2020-11-24 2022-06-11 國立成功大學 Modified metal nanoparticles, manufacturing method thereof and method for detecting manganese ion concentration

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10520462B1 (en) * 2018-11-24 2019-12-31 Alfaisal University Electrochemical screening for the selection of DNA aptamers

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BALA A ET AL.: "Electrochim. Acta, 2015; 180: 763-769", ELECTROCHEMICAL DETERMINATION OF LEAD ION WITH DNA OLIGONUCLEOTIDE-BASED BIOSENSOR USING ANIONIC REDOX MARKER. *
LEE YJ ET AL.: "Sens Actuators B Chem, 2014; 201: 535-544", A MULTIFUNCTIONAL SENSOR: CHROMOGENIC SENSING FOR MN2+ AND FLUORESCENT SENSING FOR ZN2+ AND AL3+. *
ZHOU Y AT AL.: "Talanta, 2012; 97: 331-335", COLORIMETRIC DETECTION OF MN2+ USING SILVER NANOPARTICLES COFUNCTIONALIZED WITH 4-MERCAPTOBENZOIC ACID AND MELAMINE AS A PROBE. *

Also Published As

Publication number Publication date
PL248352B1 (en) 2025-12-01
WO2025071421A1 (en) 2025-04-03

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