PL445283A1 - Sposób nieinwazyjnej analizy płodowego DNA oraz frakcji płodu z wykorzystaniem reakcji multipleks digital PCR - Google Patents

Sposób nieinwazyjnej analizy płodowego DNA oraz frakcji płodu z wykorzystaniem reakcji multipleks digital PCR

Info

Publication number
PL445283A1
PL445283A1 PL445283A PL44528323A PL445283A1 PL 445283 A1 PL445283 A1 PL 445283A1 PL 445283 A PL445283 A PL 445283A PL 44528323 A PL44528323 A PL 44528323A PL 445283 A1 PL445283 A1 PL 445283A1
Authority
PL
Poland
Prior art keywords
fetal
digital pcr
multiplex digital
invasive analysis
fractions
Prior art date
Application number
PL445283A
Other languages
English (en)
Inventor
Anna NYKEL
Agnieszka GACH
Original Assignee
Instytut Centrum Zdrowia Matki Polki
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instytut Centrum Zdrowia Matki Polki filed Critical Instytut Centrum Zdrowia Matki Polki
Priority to PL445283A priority Critical patent/PL445283A1/pl
Priority to PCT/PL2024/050042 priority patent/WO2024263045A1/en
Publication of PL445283A1 publication Critical patent/PL445283A1/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6879—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/16—Assays for determining copy number or wherein the copy number is of special importance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/165—Mathematical modelling, e.g. logarithm, ratio

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Sposób analizy wolnokrążącego płodowego cffDNA w celu determinacji płci oraz oceny frakcji płodu charakteryzujący się tym, że pobiera się materiał biologiczny od ciężarnej pacjentki, izoluje się cffDNA, ocenia się płodowe DNA, przeprowadza się reakcję multipleks digital PCR w celu amplifikacji genów na chromosomie Y obejmujących RPS4Y2, SRY, USP9Y oraz genów referencyjnych RPPH1, TERT, EIF2C1 i analizuje się dane.
PL445283A 2023-06-20 2023-06-20 Sposób nieinwazyjnej analizy płodowego DNA oraz frakcji płodu z wykorzystaniem reakcji multipleks digital PCR PL445283A1 (pl)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL445283A PL445283A1 (pl) 2023-06-20 2023-06-20 Sposób nieinwazyjnej analizy płodowego DNA oraz frakcji płodu z wykorzystaniem reakcji multipleks digital PCR
PCT/PL2024/050042 WO2024263045A1 (en) 2023-06-20 2024-06-20 Method of non-invasive fetal dna and fetal fraction analysis using multiplex digital pcr reaction

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL445283A PL445283A1 (pl) 2023-06-20 2023-06-20 Sposób nieinwazyjnej analizy płodowego DNA oraz frakcji płodu z wykorzystaniem reakcji multipleks digital PCR

Publications (1)

Publication Number Publication Date
PL445283A1 true PL445283A1 (pl) 2024-12-23

Family

ID=93936122

Family Applications (1)

Application Number Title Priority Date Filing Date
PL445283A PL445283A1 (pl) 2023-06-20 2023-06-20 Sposób nieinwazyjnej analizy płodowego DNA oraz frakcji płodu z wykorzystaniem reakcji multipleks digital PCR

Country Status (2)

Country Link
PL (1) PL445283A1 (pl)
WO (1) WO2024263045A1 (pl)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013055817A1 (en) * 2011-10-11 2013-04-18 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2018178700A1 (en) * 2017-03-31 2018-10-04 Premaitha Limited Method of detecting a fetal chromosomal abnormality

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2909479A1 (en) * 2013-05-09 2014-11-13 F. Hoffmann-La Roche Ag Method of determining the fraction of fetal dna in maternal blood using hla markers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013055817A1 (en) * 2011-10-11 2013-04-18 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2018178700A1 (en) * 2017-03-31 2018-10-04 Premaitha Limited Method of detecting a fetal chromosomal abnormality

Also Published As

Publication number Publication date
WO2024263045A1 (en) 2024-12-26

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