PL443190A1 - Method of producing a crude preparation of digestive proteases from fish - Google Patents

Method of producing a crude preparation of digestive proteases from fish

Info

Publication number
PL443190A1
PL443190A1 PL443190A PL44319022A PL443190A1 PL 443190 A1 PL443190 A1 PL 443190A1 PL 443190 A PL443190 A PL 443190A PL 44319022 A PL44319022 A PL 44319022A PL 443190 A1 PL443190 A1 PL 443190A1
Authority
PL
Poland
Prior art keywords
fish
producing
precipitate
digestive proteases
digestive
Prior art date
Application number
PL443190A
Other languages
Polish (pl)
Inventor
Patryk Kamiński
Mariusz Szymczak
Original Assignee
Zachodniopomorski Uniwersytet Technologiczny W Szczecinie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zachodniopomorski Uniwersytet Technologiczny W Szczecinie filed Critical Zachodniopomorski Uniwersytet Technologiczny W Szczecinie
Priority to PL443190A priority Critical patent/PL443190A1/en
Publication of PL443190A1 publication Critical patent/PL443190A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Biomedical Technology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Meat, Egg Or Seafood Products (AREA)

Abstract

Przedstawiony na rysunku sposób wytwarzania surowego preparatu proteaz trawiennych z ryb, zwłaszcza śledzia, z wykorzystaniem ekstrakcji enzymów i chlorku sodu, charakteryzuje się tym, że rozdrobnione przewody pokarmowe ryb miesza się z 1-10% wodnym roztworem etanolu w proporcji 1:1-10 (masa : objętość), mieszaninę homogenizuje się, a następnie oddziela osad, przy czym przed dodaniem wodnego roztworu etanolu lub po oddzieleniu osadu po homogenizacji dodaje się NaCl do uzyskania jego końcowego stężenia co najmniej 3%, miesza się, a następnie oddziela się osad. Po obu etapach uzyskuje się surowy preparat proteaz trawiennych z ryb.The method for producing a raw preparation of digestive proteases from fish, especially herring, presented in the figure, using the extraction of enzymes and sodium chloride, is characterized by the fact that the crushed digestive tracts of fish are mixed with a 1-10% aqueous solution of ethanol in a ratio of 1:1-10 ( mass: volume), the mixture is homogenized and then the precipitate is separated. Before adding the aqueous ethanol solution or after separating the precipitate after homogenization, NaCl is added to obtain its final concentration of at least 3%, stirred and then the precipitate is separated. After both stages, a crude preparation of fish digestive proteases is obtained.

PL443190A 2022-12-20 2022-12-20 Method of producing a crude preparation of digestive proteases from fish PL443190A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL443190A PL443190A1 (en) 2022-12-20 2022-12-20 Method of producing a crude preparation of digestive proteases from fish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL443190A PL443190A1 (en) 2022-12-20 2022-12-20 Method of producing a crude preparation of digestive proteases from fish

Publications (1)

Publication Number Publication Date
PL443190A1 true PL443190A1 (en) 2024-06-24

Family

ID=91618289

Family Applications (1)

Application Number Title Priority Date Filing Date
PL443190A PL443190A1 (en) 2022-12-20 2022-12-20 Method of producing a crude preparation of digestive proteases from fish

Country Status (1)

Country Link
PL (1) PL443190A1 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982003533A1 (en) * 1981-04-15 1982-10-28 Kjell Rolf Opshaug Method for enzyme maturing of fish
PL415721A1 (en) * 2016-01-11 2017-07-17 Zachodniopomorski Uniwersytet Technologiczny W Szczecinie Method for increasing proteolytic activity in a bath remaining after marinating herrings
PL426641A1 (en) * 2018-08-13 2020-02-24 Zachodniopomorski Uniwersytet Technologiczny W Szczecinie Method of pickling saltwater and freshwater fish resistant to pickling
RU2020134730A (en) * 2020-10-21 2022-04-21 Елисей Александрович Баженов METHOD FOR PRODUCING ENZYME PREPARATION WITH PROTEOLITIC ACTION

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982003533A1 (en) * 1981-04-15 1982-10-28 Kjell Rolf Opshaug Method for enzyme maturing of fish
PL415721A1 (en) * 2016-01-11 2017-07-17 Zachodniopomorski Uniwersytet Technologiczny W Szczecinie Method for increasing proteolytic activity in a bath remaining after marinating herrings
PL426641A1 (en) * 2018-08-13 2020-02-24 Zachodniopomorski Uniwersytet Technologiczny W Szczecinie Method of pickling saltwater and freshwater fish resistant to pickling
RU2020134730A (en) * 2020-10-21 2022-04-21 Елисей Александрович Баженов METHOD FOR PRODUCING ENZYME PREPARATION WITH PROTEOLITIC ACTION

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