PL441076A1 - DNA aptamer showing affinity for the PD-L1 protein and its use, functional covalent complex and its use - Google Patents

DNA aptamer showing affinity for the PD-L1 protein and its use, functional covalent complex and its use

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Publication number
PL441076A1
PL441076A1 PL441076A PL44107622A PL441076A1 PL 441076 A1 PL441076 A1 PL 441076A1 PL 441076 A PL441076 A PL 441076A PL 44107622 A PL44107622 A PL 44107622A PL 441076 A1 PL441076 A1 PL 441076A1
Authority
PL
Poland
Prior art keywords
application
covalent complex
protein
subject matter
functional covalent
Prior art date
Application number
PL441076A
Other languages
Polish (pl)
Inventor
Stanisław Malicki
Grzegorz Dubin
Piotr MYDEL
Joanna Kozieł
Barbara Pucelik
Edyta ŻYŁA
Małgorzata BENEDYK-MACHACZKA
Anna GOLDA
Wojciech GAŁAN
Marta KAMIŃSKA
Barbara CHRUŚCICKA-SMAGA
Alicja Sochaj-Gregorczyk
Katarzyna MAGIERA-MULARZ
Anna CZARNA
Original Assignee
Uniwersytet Jagielloński
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Jagielloński filed Critical Uniwersytet Jagielloński
Priority to PL441076A priority Critical patent/PL441076A1/en
Priority to PCT/PL2023/050030 priority patent/WO2023211299A1/en
Publication of PL441076A1 publication Critical patent/PL441076A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3517—Marker; Tag

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Pierwszym przedmiotem zgłoszenia jest aptamer wykazujący powinowactwo do białka PD-L1 posiadający sekwencję przedstawioną jako SEQ.1. Drugim przedmiotem zgłoszenia jest zastosowanie oligonukleotydu jak zdefiniowano w pierwszym przedmiocie zgłoszenia do wytwarzania funkcjonalnego kompleksu kowalencyjnego zawierającego znacznik fluoroscencyjny albo substancję powinowactwa i oligonukleotyd według pierwszego przedmiotu zgłoszenia. Kolejnym przedmiotem zgłoszenia jest funkcjonalny kompleks kowalencyjny zawierający znacznik albo substancję powinowactwa i oligonukleotyd jak zdefiniowano w pierwszym przedmiocie zgłoszenia. Zgłoszenie obejmuje również zastosowanie funkcjonalnego kompleksu kowalencyjnego w obrazowaniu diagnostycznym, zwłaszcza w obrazowaniu nowotworów.The first subject of the application is an aptamer showing affinity for the PD-L1 protein having the sequence shown as SEQ.1. The second subject matter of the application is the use of an oligonucleotide as defined in the first subject matter of the application for the preparation of a functional covalent complex comprising a fluorescent label or affinity substance and an oligonucleotide according to the first subject matter of the application. A further subject matter of the application is a functional covalent complex comprising a tag or affinity substance and an oligonucleotide as defined in the first subject matter of the application. The application also covers the use of the functional covalent complex in diagnostic imaging, especially in cancer imaging.

PL441076A 2022-04-30 2022-04-30 DNA aptamer showing affinity for the PD-L1 protein and its use, functional covalent complex and its use PL441076A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL441076A PL441076A1 (en) 2022-04-30 2022-04-30 DNA aptamer showing affinity for the PD-L1 protein and its use, functional covalent complex and its use
PCT/PL2023/050030 WO2023211299A1 (en) 2022-04-30 2023-05-02 Dna aptamer having affinity for pd-l1 protein and its use, functional covalent complex and its use

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL441076A PL441076A1 (en) 2022-04-30 2022-04-30 DNA aptamer showing affinity for the PD-L1 protein and its use, functional covalent complex and its use

Publications (1)

Publication Number Publication Date
PL441076A1 true PL441076A1 (en) 2023-11-06

Family

ID=87136360

Family Applications (1)

Application Number Title Priority Date Filing Date
PL441076A PL441076A1 (en) 2022-04-30 2022-04-30 DNA aptamer showing affinity for the PD-L1 protein and its use, functional covalent complex and its use

Country Status (2)

Country Link
PL (1) PL441076A1 (en)
WO (1) WO2023211299A1 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107794268B (en) * 2017-03-14 2021-03-26 湖南大学 Aptamer for detecting human PDL1 protein and application of aptamer in preparation of detection preparation
CN108929874B (en) * 2018-07-11 2021-12-14 安徽省昂普拓迈生物科技有限责任公司 Nucleic acid aptamer specifically binding to cell with high-expression PDL1 protein and application of nucleic acid aptamer
WO2021132936A1 (en) * 2019-12-27 2021-07-01 인터올리고 주식회사 Anti-cancer immunotherapeutic composition for treating cancer
WO2022000330A1 (en) * 2020-06-30 2022-01-06 首都师范大学 Method for screening dna aptamer of pd-l1 in vitro and application thereof in cancer diagnosis

Also Published As

Publication number Publication date
WO2023211299A1 (en) 2023-11-02

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