PL433162A1 - A method of marking monodisperse carrier particles and a microfluidic device for the generation of monodisperse carrier particles - Google Patents
A method of marking monodisperse carrier particles and a microfluidic device for the generation of monodisperse carrier particlesInfo
- Publication number
- PL433162A1 PL433162A1 PL433162A PL43316220A PL433162A1 PL 433162 A1 PL433162 A1 PL 433162A1 PL 433162 A PL433162 A PL 433162A PL 43316220 A PL43316220 A PL 43316220A PL 433162 A1 PL433162 A1 PL 433162A1
- Authority
- PL
- Poland
- Prior art keywords
- carrier particles
- carrier
- substrate
- monodisperse
- chemicals
- Prior art date
Links
- 239000002245 particle Substances 0.000 title abstract 22
- 238000000034 method Methods 0.000 title abstract 2
- 239000000758 substrate Substances 0.000 abstract 6
- 239000000126 substance Substances 0.000 abstract 4
- 238000003384 imaging method Methods 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 2
- 239000000725 suspension Substances 0.000 abstract 2
- 238000002372 labelling Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Przedmiotem wynalazku jest sposób znakowania monodyspersyjnych cząstek nośnika obejmujący etapy: wytworzenia cząstek nośnika w zawiesinie, nanoszenia cząstek nośnika na substrat, obrazowania, przy czym etap obrazowania obejmuje: identyfikowanie wybranej pojedynczej cząstki nośnika i/lub identyfikowanie kolejno wszystkich cząstek nośnika; gdzie na etapie wytworzenia cząstek nośnika wytwarza się zawiesinę cząstek nośnika zawierających każdą mieszaninę komórek i/lub substancji chemicznych, przy czym komórki i/lub substancje chemiczne są podawane do cząstek nośnika sekwencyjnie wg określonego protokołu w taki sposób, że kolejna wytworzona cząstka nośnika zawiera znaną mieszaninę komórek i/lub substancji chemicznych, i na etapie nanoszenia cząstek nośnika na substrat nanosi się cząstki nośnika na substrat w postaci łańcucha, znamienny tym, że cząstki nośnika (8 albo 8') zawierające komórki i/lub substancje chemiczne w zawiesinie nanosi się z igły nanoszącej (10 albo 10') jedna po drugiej na powierzchnię substratu (5 albo 5'a), przy czym gdy tempo podawania ffeed cząstek nośnika (8 albo 8') z igły nanoszącej (10 albo 10') na powierzchnię substratu (5 albo 5'a) jest równe tempu unoszenia fadv=U/D cząstek nośnika (8 albo 8') przez podłoże, gdzie U to prędkość przemieszczania się głowicy drukującej (3) a D to średnica cząstki nośnika (8 albo 8'), określone wyrażeniem fadv = ffeed, to cząstki nośnika (8 albo 8') są nanoszone w postaci liniowego łańcucha (13), przy czym gdy ffeed>fadv to cząstki nośnika (8 albo 8') są nanoszone w postaci łańcucha zawierającego sekwencje zaburzeń (13a), i na etapie obrazowania dokonuje się identyfikowania pojedynczej cząstki nośnika i/lub cząstek nośnika (8 albo 8') na podstawie wygenerowanej sekwencji zaburzeń. Wynalazek obejmuje również urządzenie do wytwarzania cząstek nośnika.The invention relates to a method of labeling monodisperse carrier particles comprising the steps of: preparing carrier particles in suspension, applying carrier particles to a substrate, imaging, the imaging step comprising: identifying a single selected carrier particle and / or identifying all carrier particles sequentially; wherein in the carrier particle preparation step a carrier particle is suspended containing each mixture of cells and / or chemicals, the cells and / or chemicals are sequentially administered to the carrier particles according to a defined protocol such that the next produced carrier particle contains a known mixture cells and / or chemicals, and in the step of applying the carrier particles to the substrate, the carrier particles are applied to the substrate in chain form, characterized in that carrier particles (8 or 8 ') containing cells and / or chemicals in suspension are applied from a needle applying (10 or 10 ') one after another to the surface of the substrate (5 or 5'a), whereby the rate of feeding the carrier particles (8 or 8') from the application needle (10 or 10 ') onto the surface of the substrate (5 or 5'a) is equal to the float rate fadv = U / D of the carrier particles (8 or 8 ') through the substrate, where U is the speed of the print head (3) and D is the particle diameter n of the axis (8 or 8 '), defined by the expression fadv = ffeed, the carrier particles (8 or 8') are applied in the form of a linear chain (13), and when ffeed> fadv then the carrier particles (8 or 8 ') are applied as a chain containing the disturbance sequences (13a), and in the imaging step, identification of a single carrier particle and / or carrier particles (8 or 8 ') from the generated disturbance sequence. The invention also includes an apparatus for producing carrier particles.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL433162A PL246693B1 (en) | 2020-03-06 | 2020-03-06 | Method of marking monodisperse carrier particles and a microfluidic device for generation of monodisperse carrier particles |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL433162A PL246693B1 (en) | 2020-03-06 | 2020-03-06 | Method of marking monodisperse carrier particles and a microfluidic device for generation of monodisperse carrier particles |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL433162A1 true PL433162A1 (en) | 2021-09-13 |
| PL246693B1 PL246693B1 (en) | 2025-02-24 |
Family
ID=77662651
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL433162A PL246693B1 (en) | 2020-03-06 | 2020-03-06 | Method of marking monodisperse carrier particles and a microfluidic device for generation of monodisperse carrier particles |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL246693B1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4559577A1 (en) | 2023-11-24 | 2025-05-28 | Instytut Chemii Fizycznej Polskiej Akademii Nauk | A device, method and a use of the device for printing ordered arrays of double-emulsion droplets at a substrate under an external aqueous phase |
-
2020
- 2020-03-06 PL PL433162A patent/PL246693B1/en unknown
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4559577A1 (en) | 2023-11-24 | 2025-05-28 | Instytut Chemii Fizycznej Polskiej Akademii Nauk | A device, method and a use of the device for printing ordered arrays of double-emulsion droplets at a substrate under an external aqueous phase |
| WO2025110888A1 (en) | 2023-11-24 | 2025-05-30 | Instytut Chemii Fizycznej Polskiej Akademii Nauk | A device, method and a use of the device for printing ordered arrays of double-emulsion droplets at a substrate under an external aqueous phase |
Also Published As
| Publication number | Publication date |
|---|---|
| PL246693B1 (en) | 2025-02-24 |
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