PL419960A1 - Method for monitoring the process of acute lymphoblastic leukaemia by means of determination of amino acids - Google Patents
Method for monitoring the process of acute lymphoblastic leukaemia by means of determination of amino acidsInfo
- Publication number
- PL419960A1 PL419960A1 PL419960A PL41996016A PL419960A1 PL 419960 A1 PL419960 A1 PL 419960A1 PL 419960 A PL419960 A PL 419960A PL 41996016 A PL41996016 A PL 41996016A PL 419960 A1 PL419960 A1 PL 419960A1
- Authority
- PL
- Poland
- Prior art keywords
- amino acids
- determination
- analysis
- gradient elution
- subject
- Prior art date
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- Investigating Or Analysing Biological Materials (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
Przedmiotem zgłoszenia jest sposób oznaczania aminokwasów w osoczu i płynie mózgowo - rdzeniowym. Przedmiotowy sposób oznaczania aminokwasów w materiale biologicznym charakteryzuje się tym, że analiza aminokwasów w materiale jest w następujących etapach: - przygotowanie próbki materiału biologicznego do analizy chromatograficznej, - rozdzielenie następuje poprzez chromatografię cieczową sprzężoną ze spektrometrią mas (LC - MS) - rozdział 21 aminokwasów następuje poprzez chromatografię oddziaływań hydrofilowych HILTC z wykorzystaniem polarnej kolumny, - faza ruchoma składała się z fazy A: 10 mM mrówczan amonu (pH3) w wodzie i B: 10 mM mrówczan amonu w acetonitrylu w warunkach elucji gradientowej, - przepływ fazy ruchomej wynosi 1 ml/min, - temperatura gazu rozpylającego: 250°C, przepływ gazu rozpylającego: 12 L/min, napięcie przyłożone do kapilary: 3 kV, napięcie fragmentacyjne zależne od substancji (zakres od 75 do 125), - czas pojedynczej analizy 21 aminokwasów wraz z równoważeniem kolumny wynosi 30 min, - rozdzielenie aminokwasów nastąpiło w warunkach elucji gradientowej. Zgłoszenie obejmuje też zastosowanie przedmiotowego sposobu do oznaczania aminokwasów u osób chorych na białaczkę limfoblastyczną w momencie postawienia diagnozy oraz podczas chemioterapii.The subject of the application is a method for determining amino acids in plasma and cerebrospinal fluid. The subject method of determining amino acids in biological material is characterized by the fact that the analysis of amino acids in the material is in the following stages: - preparation of a sample of biological material for chromatographic analysis, - separation is carried out by liquid chromatography coupled with mass spectrometry (LC - MS) - 21 amino acids are separated by HILTC hydrophilic interaction chromatography using a polar column, - the mobile phase consisted of phase A: 10 mM ammonium formate (pH3) in water and B: 10 mM ammonium formate in acetonitrile under gradient elution, - the mobile phase flow is 1 ml / min, - atomizing gas temperature: 250 ° C, atomizing gas flow: 12 L / min, voltage applied to the capillary: 3 kV, fragmentation voltage dependent on the substance (range from 75 to 125), - single analysis time 21 amino acids with balancing columns are 30 min, - amino acid separation will occur of the gradient elution conditions. The application also includes the use of the subject method for the determination of amino acids in patients with lymphoblastic leukemia at the time of diagnosis and during chemotherapy.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL419960A PL419960A1 (en) | 2016-12-22 | 2016-12-22 | Method for monitoring the process of acute lymphoblastic leukaemia by means of determination of amino acids |
| EP17840505.6A EP3559675B1 (en) | 2016-12-22 | 2017-12-21 | A method for monitoring of amino acids in biological material |
| PL17840505.6T PL3559675T3 (en) | 2016-12-22 | 2017-12-21 | A method for monitoring of amino acids in biological material |
| PCT/PL2017/000130 WO2018117881A1 (en) | 2016-12-22 | 2017-12-21 | A method for monitoring of amino acids in biological material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL419960A PL419960A1 (en) | 2016-12-22 | 2016-12-22 | Method for monitoring the process of acute lymphoblastic leukaemia by means of determination of amino acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PL419960A1 true PL419960A1 (en) | 2018-07-02 |
Family
ID=62705205
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL419960A PL419960A1 (en) | 2016-12-22 | 2016-12-22 | Method for monitoring the process of acute lymphoblastic leukaemia by means of determination of amino acids |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL419960A1 (en) |
-
2016
- 2016-12-22 PL PL419960A patent/PL419960A1/en unknown
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