PL410681A1 - Method for characterizing and identifying bacterial colonies and the axial system of digital holography for the characterization and identification of bacterial colonies - Google Patents

Method for characterizing and identifying bacterial colonies and the axial system of digital holography for the characterization and identification of bacterial colonies

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Publication number
PL410681A1
PL410681A1 PL410681A PL41068114A PL410681A1 PL 410681 A1 PL410681 A1 PL 410681A1 PL 410681 A PL410681 A PL 410681A PL 41068114 A PL41068114 A PL 41068114A PL 410681 A1 PL410681 A1 PL 410681A1
Authority
PL
Poland
Prior art keywords
bacterial colonies
characterizing
characterization
identification
digital holography
Prior art date
Application number
PL410681A
Other languages
Polish (pl)
Other versions
PL232525B1 (en
Inventor
Igor Buzalewicz
Halina Podbielska
Original Assignee
Politechnika Wrocławska
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Politechnika Wrocławska filed Critical Politechnika Wrocławska
Priority to PL410681A priority Critical patent/PL232525B1/en
Publication of PL410681A1 publication Critical patent/PL410681A1/en
Publication of PL232525B1 publication Critical patent/PL232525B1/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

Przedmiotem wynalazku jest sposób do charakteryzacji i identyfikacji kolonii bakterii, który polega na tym, że wiązkę światła z koherentnego źródła światła (1) ogniskuje się za pomocą optycznych lub optoelektronicznych modulatorów fazowych (2) na przesłonie otworowej -pinholu (3), który generuje rozbieżną wiązkę świetlną, która oświetla kolonię bakterii (4) umieszczoną na transparentnym podłożu, umieszczonym na transparentnej płytce zamocowanej w statywie z możliwością przesuwu w kierunku XYZ, następnie na detektorze (5) podaje się detekcji wynik interferencji wiązki transmitowanej/nieugiętej/nierozproszonej i ugiętej/rozproszonej na kolonii bakterii w postaci hologramu osiowego, po czym rejestruje się i analizuje w komputerze (6) dwuwymiarowy osiowy hologram cyfrowy, charakterystyczny dla danego gatunku i/lub szczepu bakterii. Przedmiotem wynalazku jest również dedykowany do tego celu osiowy układ holografii cyfrowej do charakteryzacji i identyfikacji kolonii bakterii.The subject of the invention is a method for the characterization and identification of bacterial colonies, which consists in the fact that a light beam from a coherent light source (1) is focused by means of optical or optoelectronic phase modulators (2) on the aperture - pinhole (3) that generates a divergent a light beam that illuminates the bacterial colony (4) placed on a transparent substrate, placed on a transparent plate mounted in a tripod with the possibility of sliding in the XYZ direction, then on the detector (5) detection of the result of the transmitted / unbent / diffused and bent / diffused beam interference is given on a bacterial colony in the form of an axial hologram, after which a two-dimensional axial digital hologram, characteristic of a given species and / or strain of bacteria, is recorded and analyzed on the computer (6). The invention also relates to an axial digital holography system dedicated to this purpose for characterizing and identifying bacterial colonies.

PL410681A 2014-12-30 2014-12-30 Method for characterizing and identifying bacterial colonies and the axial system of digital holography for the characterization and identification of bacterial colonies PL232525B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL410681A PL232525B1 (en) 2014-12-30 2014-12-30 Method for characterizing and identifying bacterial colonies and the axial system of digital holography for the characterization and identification of bacterial colonies

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL410681A PL232525B1 (en) 2014-12-30 2014-12-30 Method for characterizing and identifying bacterial colonies and the axial system of digital holography for the characterization and identification of bacterial colonies

Publications (2)

Publication Number Publication Date
PL410681A1 true PL410681A1 (en) 2016-02-01
PL232525B1 PL232525B1 (en) 2019-06-28

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Family Applications (1)

Application Number Title Priority Date Filing Date
PL410681A PL232525B1 (en) 2014-12-30 2014-12-30 Method for characterizing and identifying bacterial colonies and the axial system of digital holography for the characterization and identification of bacterial colonies

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PL (1) PL232525B1 (en)

Also Published As

Publication number Publication date
PL232525B1 (en) 2019-06-28

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