PL409818A1 - Method for producing (R)-flavanone - Google Patents

Method for producing (R)-flavanone

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Publication number
PL409818A1
PL409818A1 PL409818A PL40981814A PL409818A1 PL 409818 A1 PL409818 A1 PL 409818A1 PL 409818 A PL409818 A PL 409818A PL 40981814 A PL40981814 A PL 40981814A PL 409818 A1 PL409818 A1 PL 409818A1
Authority
PL
Poland
Prior art keywords
strain
medium
lavanone
per
formula
Prior art date
Application number
PL409818A
Other languages
Polish (pl)
Other versions
PL221689B1 (en
Inventor
Monika Siepka
Tomasz Janeczko
Edyta Kostrzewa-Susłow
Radosław Gniłka
Czesław Wawrzeńczyk
Original Assignee
Uniwersytet Przyrodniczy we Wrocławiu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Przyrodniczy we Wrocławiu filed Critical Uniwersytet Przyrodniczy we Wrocławiu
Priority to PL409818A priority Critical patent/PL221689B1/en
Publication of PL409818A1 publication Critical patent/PL409818A1/en
Publication of PL221689B1 publication Critical patent/PL221689B1/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Sposób wytwarzania (R)-flawanolu o wzorze 2 charakteryzuje się tym, że do przygotowanej pożywki wprowadza się szczep Fusarium oxysporum AM 13 i w momencie osiągnięcia przez szczep końcowej fazy logarytmicznego wzrostu, dodaje się racemiczny (±)-flawanon o wzorze 1, w ilości od 5 mg na 100 ml pożywki do 15 mg na 100 ml pożywki, przy czym proces transformacji mikrobiologicznej prowadzi się wodną kulturą szczepu przy ciągłym wstrząsaniu, w wyniku czego, przy udziale systemu enzymatycznego szczepu, przekształceniu ulega (S)-enancjomer substratu, po czym nieprzereagowany (R)-flawanon ekstrahuje się rozpuszczalnikiem organicznym niemieszającym się z wodą i oczyszcza chromatograficznie.The method of producing (R) -lavlavanol of formula 2 is characterized by the fact that the Fusarium oxysporum AM 13 strain is introduced into the prepared medium and when the strain reaches the final phase of logarithmic growth, racemic (±) -lavanone of formula 1 is added in an amount of 5 mg per 100 ml of medium to 15 mg per 100 ml of medium, the microbiological transformation process being carried out with an aqueous culture of the strain under constant shaking, as a result of which, with the participation of the enzyme system of the strain, the (S) -enantiomer of the substrate is transformed and then unreacted (R) -lavanone is extracted with a water-immiscible organic solvent and purified by chromatography.

PL409818A 2014-10-16 2014-10-16 Method for producing (R)-flavanone PL221689B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL409818A PL221689B1 (en) 2014-10-16 2014-10-16 Method for producing (R)-flavanone

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL409818A PL221689B1 (en) 2014-10-16 2014-10-16 Method for producing (R)-flavanone

Publications (2)

Publication Number Publication Date
PL409818A1 true PL409818A1 (en) 2015-06-08
PL221689B1 PL221689B1 (en) 2016-05-31

Family

ID=53269214

Family Applications (1)

Application Number Title Priority Date Filing Date
PL409818A PL221689B1 (en) 2014-10-16 2014-10-16 Method for producing (R)-flavanone

Country Status (1)

Country Link
PL (1) PL221689B1 (en)

Also Published As

Publication number Publication date
PL221689B1 (en) 2016-05-31

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