PL397536A1 - Oligonucleotide primer, DNA sequence with recognition sites for the restriction NdeI and XhoI enzymes, expression vector and method for its preparation, recombinant strain of Escherichia coli BL21 (DE3) pLysS and the method for its preparation and a method of producing recombinant protein trehalose synthase (ST), wild type Deinococcus geothermalis DSMZ 11300 - Google Patents

Oligonucleotide primer, DNA sequence with recognition sites for the restriction NdeI and XhoI enzymes, expression vector and method for its preparation, recombinant strain of Escherichia coli BL21 (DE3) pLysS and the method for its preparation and a method of producing recombinant protein trehalose synthase (ST), wild type Deinococcus geothermalis DSMZ 11300

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Publication number
PL397536A1
PL397536A1 PL397536A PL39753611A PL397536A1 PL 397536 A1 PL397536 A1 PL 397536A1 PL 397536 A PL397536 A PL 397536A PL 39753611 A PL39753611 A PL 39753611A PL 397536 A1 PL397536 A1 PL 397536A1
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PL
Poland
Prior art keywords
dsmz
preparation
escherichia coli
plyss
trehalose synthase
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Application number
PL397536A
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Polish (pl)
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PL229056B1 (en
Inventor
Pawel Filipkowski
Józef Synowiecki
Anna Panek
Olga Pietrow
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Politechnika Gdanska
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Application filed by Politechnika Gdanska filed Critical Politechnika Gdanska
Priority to PL397536A priority Critical patent/PL229056B1/en
Publication of PL397536A1 publication Critical patent/PL397536A1/en
Publication of PL229056B1 publication Critical patent/PL229056B1/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Przedmiotem wynalazku jest starter oligonukleotydowy zsyntetyzowany w znany sposób do reakcji amplifikacji DNA o symbolu DgTreXRTAA, jak równiez sekwencja DNA otrzymana w wyniku amplifikacji DNA zawierajaca fragment DNA kodujacy bialko syntazy trehalozy Deinococcus geothermalis DSMZ 11300 oraz wektor ekspresyjny zawierajacy wklonowane DNA z fragmentem kodujacym bialko syntazy trehalozy Deinococcus geothermalis DSMZ 11300. Przedmiotem wynalazku jest tez sposób otrzymywania rekombinantowego szczepu Escherichia coli TOP10F' o symbolu Escherichia coli TOP10F'-pET30Ek/LICDgeoST charakteryzujacy sie tym, ze uzyskuje sie fragment DNA kodujacy bialko syntazy trehalozy Deinococcus geothermalis DSMZ 11300 poprzez amplifikacje DNA z zastosowaniem znanego startera DgeoSTNdeF oraz zastrzeganego w tym zgloszeniu DgTXRTAA oraz sposób otrzymywania rekombinantowego szczepu Escherichia coli BL21 (DE3) pLysS o symbolu Escherichia coli BL21 (DE3) pLysS-pET30Ek/LICDgeoST charakteryzujacy sie tym, ze uzyskuje sie fragment DNA kodujacy bialko syntazy trehalozy Deinococcus geothermalis DSMZ 11300, a takze sposób wytwarzania bialka syntazy trehalozy Deinococcus geothermalis DSMZ 11300 charakteryzujacy sie tym, ze prowadzi sie hodowle komórek szczepu bakterii o symbolu Escherichia coli BL21 (DE3) pLysS-pET30Ek/LICDgeoST.The subject of the invention is an oligonucleotide primer synthesized in a known manner for a DNA amplification reaction with the symbol DgTreXRTAA, as well as a DNA sequence obtained as a result of DNA amplification containing a DNA fragment encoding the Dehalococcus geothermalis DSMZ 11300 trehalose synthase protein and an expression vector containing the De-clocosed DNA containing geothermalis DSMZ 11300. The subject of the invention is also a method for obtaining a recombinant Escherichia coli TOP10F 'strain with the symbol Escherichia coli TOP10F'-pET30Ek / LICDgeoST characterized by obtaining a DNA fragment encoding a Deinococcus geothermalis trehalose synthase protein using known amplifier DSMZ 11300 DgeoSTNdeF and DgTXRTAA claimed in this application and the method of obtaining the recombinant Escherichia coli BL21 (DE3) pLysS strain with the symbol Escherichia coli BL21 (DE3) pLysS-pET30Ek / LICDgeoST characterized by a DNA fragment encoding the Deinococcus geothermalis DSMZ 11300 trehalose synthase protein is obtained, as well as a method for producing the Deinococcus geothermalis DSMZ 11300 trehalose synthase protein characterized in that the cells of the bacterial strain strain Escherichia coli BL21 (DE3) pLysS-LICSk pLysS-pICgek.

PL397536A 2011-12-22 2011-12-22 Oligonucleotide primer, DNA sequence with recognition sites for the restriction NdeI and XhoI enzymes, expression vector and method for its preparation, recombinant strain of Escherichia coli BL21 (DE3) pLysS and the method for its preparation and a method of producing recombinant protein trehalose synthase (ST), wild type Deinococcus geothermalis DSMZ 11300 PL229056B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL397536A PL229056B1 (en) 2011-12-22 2011-12-22 Oligonucleotide primer, DNA sequence with recognition sites for the restriction NdeI and XhoI enzymes, expression vector and method for its preparation, recombinant strain of Escherichia coli BL21 (DE3) pLysS and the method for its preparation and a method of producing recombinant protein trehalose synthase (ST), wild type Deinococcus geothermalis DSMZ 11300

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL397536A PL229056B1 (en) 2011-12-22 2011-12-22 Oligonucleotide primer, DNA sequence with recognition sites for the restriction NdeI and XhoI enzymes, expression vector and method for its preparation, recombinant strain of Escherichia coli BL21 (DE3) pLysS and the method for its preparation and a method of producing recombinant protein trehalose synthase (ST), wild type Deinococcus geothermalis DSMZ 11300

Publications (2)

Publication Number Publication Date
PL397536A1 true PL397536A1 (en) 2013-06-24
PL229056B1 PL229056B1 (en) 2018-06-29

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PL397536A PL229056B1 (en) 2011-12-22 2011-12-22 Oligonucleotide primer, DNA sequence with recognition sites for the restriction NdeI and XhoI enzymes, expression vector and method for its preparation, recombinant strain of Escherichia coli BL21 (DE3) pLysS and the method for its preparation and a method of producing recombinant protein trehalose synthase (ST), wild type Deinococcus geothermalis DSMZ 11300

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PL229056B1 (en) 2018-06-29

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