PL2678443T3 - Sposób ilościowego określania ludzkiego DNA przy użyciu kontroli wewnętrznej - Google Patents

Sposób ilościowego określania ludzkiego DNA przy użyciu kontroli wewnętrznej

Info

Publication number
PL2678443T3
PL2678443T3 PL12709012T PL12709012T PL2678443T3 PL 2678443 T3 PL2678443 T3 PL 2678443T3 PL 12709012 T PL12709012 T PL 12709012T PL 12709012 T PL12709012 T PL 12709012T PL 2678443 T3 PL2678443 T3 PL 2678443T3
Authority
PL
Poland
Prior art keywords
internal control
human dna
quantifying human
quantifying
dna
Prior art date
Application number
PL12709012T
Other languages
English (en)
Inventor
Pasquale Francesca Di
Holger Engel
Sascha Strauss
Nicola Jo Thelwell
Original Assignee
Qiagen Gmbh
Qiagen Manchester Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh, Qiagen Manchester Limited filed Critical Qiagen Gmbh
Publication of PL2678443T3 publication Critical patent/PL2678443T3/pl

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6879Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/151Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00Reactions characterised by their quantitative nature
    • C12Q2545/10Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/101Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00Nucleic acid detection characterised by assay method
    • C12Q2561/113Real time assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/166Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PL12709012T 2011-02-21 2012-02-20 Sposób ilościowego określania ludzkiego DNA przy użyciu kontroli wewnętrznej PL2678443T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11155178 2011-02-21
US201261588975P 2012-01-20 2012-01-20
EP12709012.4A EP2678443B1 (en) 2011-02-21 2012-02-20 Method for quantifying human dna using an internal control

Publications (1)

Publication Number Publication Date
PL2678443T3 true PL2678443T3 (pl) 2017-06-30

Family

ID=44279774

Family Applications (1)

Application Number Title Priority Date Filing Date
PL12709012T PL2678443T3 (pl) 2011-02-21 2012-02-20 Sposób ilościowego określania ludzkiego DNA przy użyciu kontroli wewnętrznej

Country Status (6)

Country Link
US (1) US9328385B2 (pl)
EP (1) EP2678443B1 (pl)
JP (1) JP6021230B2 (pl)
CN (1) CN103534356B (pl)
PL (1) PL2678443T3 (pl)
WO (1) WO2012113577A1 (pl)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170002397A1 (en) * 2014-01-28 2017-01-05 Qiagen Gmbh Internal amplification control
CN104911260A (zh) * 2015-04-28 2015-09-16 凯杰(苏州)转化医学研究有限公司 一种新型成纤维细胞生长因子19(fgf19)基因拷贝数检测试剂盒
EP3512962A1 (en) * 2016-09-16 2019-07-24 QIAGEN GmbH Method for determining nucleic acid degradation in a sample in which at least two overlapping amplicons are produced and two probes are used in the method
WO2018054783A1 (en) * 2016-09-16 2018-03-29 Qiagen Gmbh Method for quantifying and/or detecting human male dna
US11345952B2 (en) 2016-09-16 2022-05-31 Qiagen Gmbh Internal amplification control
CN110023508A (zh) * 2016-10-19 2019-07-16 塞缪尔·威廉姆斯 通过快速lamp分析进行染色体评估
CN108103154A (zh) * 2017-12-29 2018-06-01 武汉艾德士生物科技有限公司 判断基因组中目标基因拷贝数的方法
CN109988850B (zh) * 2019-04-29 2023-04-25 孙雷 一种快速检测人所有23对染色体数目的试剂盒

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3223104A1 (de) 1982-06-21 1983-12-22 Hoechst Ag, 6230 Frankfurt Photopolymerisierbares gemisch und damit hergestelltes photopolymerisierbares kopiermaterial
AT401270B (de) * 1994-09-26 1996-07-25 Immuno Ag Verfahren zur quantifizierung von genomischer dna
WO2003075837A2 (en) 2002-03-04 2003-09-18 U.S. Army Medical Research And Materiel Command Internal positive control for probe-based nucleic acid molecule assays and methods of making and using therof
US7250496B2 (en) * 2002-11-14 2007-07-31 Rosetta Genomics Ltd. Bioinformatically detectable group of novel regulatory genes and uses thereof
US7374927B2 (en) 2004-05-03 2008-05-20 Affymetrix, Inc. Methods of analysis of degraded nucleic acid samples
DE602007027948T8 (de) 2006-04-27 2013-04-25 Vytal Diagnostics Ab Verfahren und kit zur molekularen chromosomenquantifizierung
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
CN103180460A (zh) * 2010-09-23 2013-06-26 凯杰有限公司 用于检测和/或定量人类dna的方法

Also Published As

Publication number Publication date
WO2012113577A1 (en) 2012-08-30
JP6021230B2 (ja) 2016-11-09
CN103534356B (zh) 2016-03-09
CN103534356A (zh) 2014-01-22
EP2678443B1 (en) 2016-11-23
EP2678443A1 (en) 2014-01-01
JP2014506794A (ja) 2014-03-20
US20140147843A1 (en) 2014-05-29
US9328385B2 (en) 2016-05-03

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