PL209937B1 - The method of stimulation of growth and diagnostics of microorganisms in materials and a device for application of this method - Google Patents

Description

Description of the invention

The subject of the invention is a device for selective growth stimulation and diagnostics of microorganisms in materials, enabling automatic initiation of the growth rate of microorganisms in samples of body fluids or industrial raw materials placed on differentiating substrates in a laboratory incubator. The uneven growth of microorganisms in various conditions makes it impossible to quickly assess their biological characteristics and individual assessment of sensitivity to antibiotics, antifungal drugs and cytostatics.

Microbiological contamination of raw materials used in the pharmaceutical, food and biotechnology industries is a significant cause of losses related to the possibility of contamination of consumers and biological degradation of the stored product. The problem is exacerbated very clearly in situations where the technological procedure of production does not take into account effective methods of sterilization of both the raw materials and the product, as well as in the reality of a low sanitary level of the staff, low standard of the plant's buildings, in which the presence of fungal spores, mites and rodents that can carry bacteria.

The existing state of the art does not cover the above-mentioned problems in a simultaneous and sufficient manner, mainly in terms of the conditions for selecting their growth, diagnostics of microorganisms and the assessment of sensitivity to antibiotics and cytostatic drugs. A known technical solution is a simple incubator made of glass, polymer or metal, provided with a thermostat and a humidifying system. Another technical solution used is an ultrathermostat, which is a well-insulated, thermally insulated water container, inside which a thermostat is installed and insulated chambers, especially in which microorganisms can be grown on various media. The device is not equipped with a direct sample monitoring system, but it is suitable for typical microbiological and pharmaceutical applications. Yet another known solution is incubators with a system to control the composition of the internal atmosphere, especially in the area of oxygen and carbon dioxide.

US 2003/0113832 describes a method and a device for the diagnosis and growth stimulation of viable cells contained in a liquid submersible medium. A significant limitation of this system is the fact that the principle of its operation, both in the stimulating, inhibiting and measuring mode, is conditioned only by galvanic contact of the electrodes with the liquid medium containing the cells. A similar limitation occurs in the diagnostic scope of the discussed solution.

GB 2335981 describes a fast method of detecting microorganisms, the principle of which is based on the coupling of two diagnostic paths. The disadvantage of this solution is the fact that the first line of diagnostics (and stimulation) of cells in the liquid medium was carried out based on the measurement of zeta-potential, which depends on galvanic coupling with the liquid medium, while the second track, which is used for recording and advanced processing image of microscopic objects, in order to obtain information about their behavioral specificity, i.e. the path and dynamics of movement, is a very expensive system, requiring fast cameras, even faster image converters, expensive software and equally expensive computers with a multi-path data processing system.

When assessing the prior art, Polish patents PL 198449, PL 188833 and PL 126406 can also be taken into account.

Further inventive solutions enabling the registration of the image of the tested sample with the use of mini cameras are known, for example, from the publications: (1) Dyszkiewicz A., Koprowski R., Wróbel Z. entitled: "Computerized system for monitoring cell growth in biotechnological reactors, 1st National Congress of Biotechnology, p. 232, Ed. Half. Wrocławska, Wrocław 1999; (2) Dyszkiewicz A., Koprowski R., Wróbel Z. Fri. "Microcomputer chamber controller for experimental laboratory preparation, 1st National Congress of Biotechnology in Wrocław and on September 20-25, 1999. 233; (3) Dyszkiewicz A., Koprowski R., Wróbel Z. entitled: “System for the preparation of toxic substances; (4) Krzanowski M., Dyszkiewicz A. entitled: "Chamber for the preparation of cytostatic drugs, Urz. Stalemate. RP 1994, W 56118; (5) Krzanowska H., Preibisch J., Korda P. pt. "Laboratory animals, breeding and use, PZWL Warsaw 1974; (6) Loomer P., Ellen R., Tanenbaum H. pt: "Osteogenic and osteoclastic cell interaction development of a co-culture system, Cell Tiss Res 1998 Oct, 284 (1), 99-108: (7) Sloan A ., Shelton R., Hann A. Fri: "An in vitro approach for the study of dentinogenesis by organ culture of the dentine pulp complex from rat incisor teeth, Arch Oral Biol 1998 Jun, 43 (6), 421-430; (8) Townes-Anderson E., St Jules R.

PL 209 937 B1 pt: Micromanipulation of retinal neurons by optical tweezers. Mol Vis 1998 Jul 30, 4, 12; (9) Wartenberg M., Gunther J., Hescheler J. Fri: The embryoid body as a novel in vitro assay system for antiangiogenic.

To sum up, in classical, known microbial culture systems, the weight of the selection process rests on the chemical composition of the media used, the effect of which is supported by the stabilization of temperature, humidity and, in some cases, gas composition change. This mode of operation makes research dependent on the constant distribution of expensive, highly diversified microbiological media.

The task of the present invention is to develop a device enabling further improvement of the method of detecting microorganisms in materials, determining optimal conditions for their growth dynamics and obtaining high specificity in the differential culture of microorganisms on simple and cheap media, while increasing the speed of diagnosis and reducing costs, as well as replacing expensive , previously used chemical selectors, using cheap physical selectors.

The subject of the invention is a device for stimulating the growth and diagnostics of microorganisms in materials, essentially consisting of mutually cooperating laboratory and control and measurement modules, of which the laboratory module consists of a temperature stabilizing system, in which there is a bidirectional heat pump with an internal heat sink and external, from a gas dosing system including an oxygen valve with an oxygen dosing line, a carbon dioxide valve with a dispenser, an additional valve with an additional dispenser, from the image recording system, which includes a camera, lighting device with a light source and reflector, transparent top, on which is a Petri dish with the tested material, a diffraction grating, an optical condenser, a linear drive, a rotating disc, filters and a disc drive, and a stimulating system including an electromechanical transducer, a magnetic field coil and an electric field electrode. The control and measurement module of the device includes a multiplexer connected on one side with an analog-to-digital converter transmitting data from sensors of light, temperature, oxygen, carbon dioxide, additional gas and a digital-to-analog converter controlling the heat pump, electromechanical converter, magnetic field coil, electrodes electric field, linear drive, disc stepper drive, ultraviolet lamp, light source with reflector, oxygen valve, carbon dioxide valve, auxiliary valve, and on the other hand it is connected by a link to the computer.

Samples of the test substance, bacterial colonies or antibiotic discs are in the field of view of a camera or other electromagnetic detector installed in the incubator, which, in cooperation with the appropriate software option, sends images for processing at user-defined intervals. Images of bacterial colonies on the media are automatically indexed and the surface area assessed. These data make it possible to create graphs of the increase in the surface area of individual colonies over time, providing information on the condition of the colony. If a sample of the test substance contains bacteria or somatic cells capable of reproducing on a given type of medium and under conditions sustained by the incubator, the surface area occupied by the sample and the graph indices increase systematically. This is clear information for the operator to indicate that the test sample contains live and multiplying bacteria. The use of planimetric analysis eliminates the subjectivity of the description, as well as greatly increases the sensitivity of the method. A seasoned analyst notices planimetric changes of samples after 12-24 hours of incubation, and the computer program is able to interpret them after 3-6 hours. This gives great advantages, especially in medical applications and the possibility of unquestionable savings of electricity necessary for the operation of the incubator, increasing, for example, the flexibility of the plant's raw materials turnover, detection of contaminated supplies, detection of a fresh outbreak of infection among staff and effective monitoring of microbiological contamination of process water.

The device according to the invention enables the use of a method of stimulating the growth and diagnostics of microorganisms in a material, in which, by applying simultaneous, controlled influence on such material of selected constellations of growth selectors, such as gas composition-temperature-mechanical vibrations, gas composition-temperature-mechanical vibrations-electromagnetic wave, gas composition - temperature - mechanical vibrations - electromagnetic wave - electric field, gas composition - temperature - mechanical vibrations - electromagnetic wave - electric field - magnetic field, gas composition - temperature - electromagnetic wave, gas composition - temperature - electromagnetic wave - electric field, gas composition - temperature - electromagnetic wave - magnetic field, gas composition - temperature - electromagnetic wave - electric field - magnetic field, gas composition - temperature - mechanical vibration - field

Electrical, gas composition-temperature-mechanical vibrations-magnetic field, gas composition-temperature-mechanical vibrations-electric field-magnetic field, preferably according to the standards 1-6, both qualitative growth selection as well as qualitative information about cells and quantitative information about the vital parameters of selected cells.

Examples of particularly advantageous inter-synchronization of these selectors with respect to different types of bacteria are shown in Formulas 1-6 below.

Exemplar 1. Effect of the simultaneous application of electric, magnetic and mechanical vibrations on different shapes of bacteria:

bacteria rod-shaped bacteria oval bacteria spherical weak electric field +++ ++ + strong electric field + + ++ weak electric field HF + ++ +++ strong HF electric field - ± + weak permanent magnetic field + ++ ++ strong permanent magnetic field ± ± + weak pulsating magnetic field ++ ++ +++ strong pulsating magnetic field - ± + weak mechanical vibrations +++ ++ ++ strong mechanical vibrations ++ +++ +++ low HF mechanical vibrations + ++ +++ strong mechanical vibrations - ± +

The table contains the results of experimental studies showing the simultaneous stimulating effect of a weak electric field of low frequency (MW) on large bacteria, weaker ones on small bacteria, while a strong and higher frequency field (HF) inhibits the growth of large rod and protozoa, stimulating small bacteria . The same is true for mechanical vibrations. The mechanism of this phenomenon is related to the greater accumulation of mechanical energy or electromotive force (EMF) in a much larger body of a rodent species than a dozen or so times smaller spherical bacteria.

Exemplary 2. The influence of the simultaneous application of electric and magnetic fields and mechanical vibrations as well as high oxygen concentration on various shapes of bacteria with an aerobic and anaerobic metabolic pathway.

oxygen Dioxide coal Bacteria oxygen chopsticks Bacteria oxygen spherical Bacteria anaerobic chopsticks Bacteria anaerobic spherical weak electric field + - ++++ ++ - - strong electric field + - +++ +++ --- - weak electric field HF + - ++ ++++ - --- strong HF electric field + - ± ++ - weak permanent magnetic field + - ++ +++ - --- strong permanent magnetic field + - ± ++ --- weak pulsating magnetic field + - +++ ++++ --- - strong pulsating magnetic field + - ± ++ - weak mechanical vibrations + - ++++ +++ - --- strong mechanical vibrations + - +++ +++ --- --- low HF mechanical vibrations + - ++ ++++ --- - strong mechanical vibrations + - ± ++ -

The table contains the results of experimental tests showing the simultaneous, stimulating effect of a weak electric and magnetic field and mechanical vibrations of low frequency (MW) and high frequency (RF) in an oxygen atmosphere. The stimulating effect of weak fields and low-frequency vibrations for rodents is intensified by the stimulating effect of oxygen on aerobic bacteria and the inhibitory effect on anaerobic bacteria. Strong fields and vibrations of higher frequencies intensify the effects in small, spherical bacteria.

Exemplary 3. Effect of the simultaneous application of electric and magnetic fields and mechanical vibrations as well as a high concentration of carbon dioxide on various shapes of bacteria with an aerobic and anaerobic metabolic pathway.

oxygen Dioxide coal Bacteria oxygen chopsticks Bacteria oxygen spherical Bacteria anaerobic chopsticks Bacteria anaerobic spherical weak electric field - + ++ ++++ - - strong electric field - + +++ +++ - --- weak electric field HF - + ++++ ++ --- - strong HF electric field - + ++ ± - weak permanent magnetic field - + +++ ++ --- - strong permanent magnetic field - + ++ ± --- weak pulsating magnetic field - + ++++ +++ - --- strong pulsating magnetic field - + ++ ± - weak mechanical vibrations - + +++ ++++ --- - strong mechanical vibrations - + +++ +++ --- --- low HF mechanical vibrations - + ++++ ++ - --- strong mechanical vibrations - + ++ ± -

The table contains the results of experimental studies showing the simultaneous, stimulating effect of a weak electric and magnetic field and mechanical vibrations of low frequency (MW) and high frequency (RF) in the atmosphere of carbon dioxide. The stimulating effect of weak fields and low-frequency vibrations for rodents is intensified by the stimulating effect of carbon dioxide on anaerobic bacteria and the inhibitory effect on aerobic bacteria. Strong fields and vibrations of higher frequencies intensify the effects of small, spherical bacteria.

Exemplary 4. The influence of the simultaneous application of electric and magnetic fields and mechanical vibrations as well as high concentration of carbon dioxide under ultraviolet illumination on various shapes of bacteria with an aerobic and anaerobic metabolic pathway.

oxygen Dioxide coal Light ultraviolet. Bacteria oxygen chopsticks Bacteria oxygen spherical Bacteria anaerobic chopsticks Bacteria anaerobic spherical 1 2 3 4 5 6 7 8 weak electric field - + + - --- + ++ strong electric field - + + --- - ++ ++ weak electric field HF - + + - +++ ± strong HF electric field - + + ± - weak permanent magnetic field - + + - + ± strong permanent magnetic field - + + - ± - weak pulsating magnetic field - + + --- - ++ + strong pulsating magnetic field - + + + - weak mechanical vibrations - + + - ++ +++

PL 209 937 B1 cont. table

1 2 3 4 5 6 Ί 8 strong mechanical vibrations - + + - - ++ + low HF mechanical vibrations - + + - +++ + strong mechanical vibrations - + + + -

The table contains the results of experimental studies showing the simultaneous, stimulating effect of the weak electric and magnetic field and mechanical vibrations of low frequency (MW) and high frequency (HF) in the atmosphere of carbon dioxide, with simultaneous UV illumination. The stimulating effect of weak fields and low frequency vibrations for rodents is intensified by the stimulating effect of carbon dioxide on anaerobic bacteria and strongly inhibited by ultraviolet. In the case of aerobic bacteria, the action of fields and vibrations intensifies the absorption of carbon dioxide, which is deadly for aerobes, which is additionally intensified by ultraviolet. Strong fields and vibrations of higher frequencies intensify the effects in small, spherical bacteria.

Exemplary 5. Effect of simultaneous application of electric and magnetic fields and mechanical vibrations and high concentration of carbon dioxide under infrared illumination on various shapes of bacteria with aerobic and anaerobic metabolic pathways.

oxygen Two- oxide coal Light under- June Bacteria oxygen chopsticks Bacteria oxygen spherical Bacteria anaerobic chopsticks Bacteria anaerobic spherical weak electric field - + + - - ++ +++ strong electric field - + + - --- +++ +++ weak electric field HF - + + --- - ++++ + strong HF electric field - + + - - + ± weak permanent magnetic field - + + - --- +++ + strong permanent magnetic field - + + --- --- + ± weak pulsating magnetic field - + + - - +++ ++ strong pulsating magnetic field - + + - --- ++ + weak mechanical vibrations - + + - --- +++ ++++ strong mechanical vibrations - + + - - +++ ++ low HF mechanical vibrations - + + - - ++++ ++ strong mechanical vibrations - + + - - ++ ±

The table contains the results of experimental studies showing the simultaneous, stimulating effect of a weak electric and magnetic field and mechanical vibrations of low frequency (MW) and high frequency (HF) in the atmosphere of carbon dioxide with simultaneous infrared illumination. The stimulating effect of weak fields and low-frequency vibrations for rodents is intensified by the stimulating effect of carbon dioxide on anaerobic bacteria and additionally intensified by infrared. In the case of aerobic bacteria, the action of fields and vibrations intensifies the absorption of carbon dioxide, which is deadly for aerobes, which is partially mitigated by infrared. Strong fields and vibrations of higher frequencies intensify the effects in small, spherical bacteria.

Exemplar 6. Effect of the simultaneous application of electric and magnetic fields and mechanical vibrations and high oxygen concentration under infrared illumination on different shapes of bacteria with an aerobic and anaerobic metabolic pathway.

PL 209 937 B1

oxygen Two- oxide coal Light under- June Bacteria oxygen chopsticks Bacteria oxygen spherical Bacteria anaerobic chopsticks Bacteria anaerobic spherical weak electric field + - + +++ ++++ - strong electric field + - + +++ +++ - - weak electric field HF + - + +++++ +++ - - strong HF electric field + - + +++ ++ - weak permanent magnetic field + - + ++++ ++ --- --- strong permanent magnetic field + - + ++ + - --- weak pulsating magnetic field + - + ++++ +++ - - strong pulsating magnetic field + - + +++ ++ - --- weak mechanical vibrations + - + ++++ +++++ --- --- strong mechanical vibrations + - + ++++ ++ - -

The table contains the results of experimental studies showing the stimulating effect of a weak electric and magnetic field and mechanical vibrations of low frequency (MW) and high frequency (RF) in an oxygen atmosphere with simultaneous ultraviolet illumination. The stimulating effect of weak fields and low frequency vibrations for rodents is intensified by the stimulating effect of oxygen on aerobic bacteria and strongly stimulated by infrared. In the case of anaerobic bacteria, the action of fields and vibrations intensifies the absorption of oxygen, which is deadly for anaerobes, which is additionally intensified by infrared. Strong fields and vibrations of higher frequencies intensify the effects in small, spherical bacteria.

The device according to the invention, as can be seen from the above discussion, description and standards, enables efficient detection of microorganisms in materials and determination of their growth dynamics, without the need to use expensive chemical agents. It also allows for high specificity in the differential culture of microorganisms on simple and cheap media.

The device forming the subject of the invention is shown in its embodiment in a diagram showing its construction in a schematic layout. According to the invention, the device for stimulating the growth and diagnosis of microorganisms in materials consists of a laboratory module A and a control and measurement module B.

The laboratory module A is made of the shell 1 of the incubator, delimiting its space 2, where the rack 4 of the table is covered with a transparent top 3, on which there is a Petri dish 32 with the culture. Around the edge of the transparent top 3 is a magnetic field coil 5 and an electric field coil 5 inside the electrode. One or more electromechanical transducers 7 causing vibrations are attached to the edge of the transparent table top 3. A thermal pump 10 is built into the wall of the shell 1 of the incubator, which is provided with an external heat sink 8 and an internal heat sink 7. The external heat sink 8 may be provided with a fan. Above the table top 3, in the heater jacket 1 there is a lighting sensor 11, a temperature sensor 12, an oxygen concentration sensor 13, a carbon dioxide concentration sensor 14, a gas concentration sensor 15 and a TV camera 16. Under the transparent top 3 there is a tripod 17 of the lighting device on which the rotating disc 19 with transmission filters 18 is mounted, mounted on the axis of the stepper motor 20. Above the disc 19, there is a diffractive grating 21, the angle of inclination of which is adjusted by means of a linear drive 22. Above the diffractive grating 21, within a transparent table 3 there is an optical condenser 23. The transparent table top 3 is illuminated from below from a light source 24 with a reflector in which there may be incandescent light sources and luminescent sources emitting different wavelengths of light. An oxygen valve 25 is mounted in the jacket 1 of the incubator, through which oxygen from a cylinder, not shown, is supplied through a dosing line 26 to the inner space 2. In the jacket 1 of the incubator, a carbon dioxide valve 27 is fitted, through which gas from a cylinder, not shown, is in the drawing, it is fed through a dosing line 28 to the inner space 2. An additional valve 29 is also mounted in the jacket 1 of the incubator for any gas, through which a cylinder, not shown in the drawing, is fed through the dosing line 30

Additional gas. In the vicinity of the transparent top 3 there is an ultraviolet burner 31 with a fan, which enables the sterilization of the interior of the incubator.

The control and measurement module B consists of a multiplexer 33 connected to a computer or an autonomous processor 54 via link 34. The multiplexer 33 is connected to an analog-to-digital converter 35, which is connected to: cable 36 connecting the television camera 16, cable 37 of the lighting sensor 11, line 38 of temperature sensor 12, line 39 of oxygen sensor 13, line 40 of carbon dioxide sensor 14, and line 41 of the additional sensor. The multiplexer 33 is connected to a digital-to-analog controller 42 from which leads 43 to control the electromechanical converter, 44 to control the electric field, 45 to control the magnetic field, 46 to control the oxygen valve, 47 to control the carbon dioxide valve, 48 to control the auxiliary valve , a UV burner control wire 49, a disc stepper motor 20 control wire 50, a control wire 51 for controlling the line drive 22 of the diffractive grating 21, a wire 52 for controlling the heat pump 10, and a wire 53 for controlling the light source 24.

The operation of the device for the stimulation and diagnosis of microbial growth in materials consists in creating specific conditions in the inner space 2 in terms of the concentration of oxygen and / or carbon dioxide and / or any chosen gas, temperature and / or lighting (with a specific wavelength) and / or magnetic field and / or electric field and / or mechanical vibrations, which strongly inhibit the growth of most bacterial strains, while maintaining the maximum growth rate of the selected strain. The rate of growth of bacterial colonies on the medium is proportional to the increase in the area of the colony per unit time, hence the phenomenon is optically recorded with a camera and interpreted quantitatively by a counting program. After the computer or microprocessor 54 is turned on, measurements are made of the interior space 2 of the incubator by means of a light sensor 11, a temperature sensor 12, an oxygen concentration sensor 13, a carbon dioxide concentration sensor 14, and a selected gas sensor 15. The information from the light sensor 11 is transmitted via line 37 to an analog-to-digital converter 35, then to the multiplexer 33 and the computer 54, where it is compared with a reference value set in the program. The occurring difference between the expected light intensity and the wavelength triggers a program sequence which, through the multiplexer 33 and the digital-to-analog converter 35, runs through the conduit 53 the corresponding option of the light source 24 with the reflector, in terms of intensity and wavelength. This light can be further filtered in a stepwise manner, using transmission filters 18 located on the rotating disk 19, driven by a stepper motor 20, controlled by a wire 50. Smooth regulation of the length of the light wave is possible by means of a diffractive grating 21, the angle of which is regulated by a linear drive 22 , controlled cable 51.

After obtaining the set parameters, the light passes through the condenser 23 located in the transparent table top 3, supported on the table frame 4 and illuminates the Petri dish 32 with the culture placed on it. On the transparent top 3, along the edge, a magnetic field coil 5 powered by a magnetic field control line 45, electric field electrodes 6 powered by an electric field control line 44 and a converter or electromechanical transducers 7 powered by a control line 43 for an electromechanical converter are installed. Changes in the parameters of the intensity and frequency of the magnetic field, electric field and mechanical vibrations of the Petri dish 32 with the culture constitute an additional selection factor for bacterial growth.

The information from the temperature sensor 12 is sent via line 38 to an analog-to-digital converter 35, then to multiplexer 33 and computer 54, where it is compared with a reference value set in the program. The occurring difference between the expected temperature triggers a program sequence which, via the multiplexer 33 and the digital-to-analog converter 42, activates the appropriate option of the heat pump 10, for example a Peltier module, via line 52. If the temperature in the inner space 2 is lower than the set temperature, the internal heat sink 9 heats up causing the temperature to rise, while when the temperature is higher than the set temperature, the external heat sink 8 heats up, taking heat from the interior of the incubator. The information from the oxygen concentration sensor 13 is sent via a line 39 to an analog-to-digital converter 35, then to a multiplexer 33 and a computer 54, where it is compared with a reference value set in the program. The present difference in concentration triggers a program sequence which, via the multiplexer 33 and the digital-to-analog converter 42, actuates the oxygen valve 25 through line 46, from which through line 26 oxygen enters the inner space 2.

Information from the carbon dioxide sensor 14 is sent via a line 40 to an analog-to-digital converter 35, then to a multiplexer 33 and a computer 54, where it is compared

PL 209 937 B1 with the reference value set in the program. The present difference in concentrations activates the program sequence which, through the multiplexer 33 and the digital-to-analog converter 42, activates the carbon dioxide valve 27 via line 47, from which gas enters the inner space 2 of the incubator through the carbon dioxide dosing line 28. Information from the auxiliary sensor 15 is sent via line 41 to an analog-to-digital converter 35, then to multiplexer 33 and computer 54, where it is compared with a reference value set in the program. The occurring difference activates the program sequence, which, through the multiplexer 33 and the digital-to-analog converter 42, activates an additional valve 29 through the conduit 48, from which additional gas enters the inner space through the dosing conduit 2. The petri dish 32 with the culture is in the field of view of the camera. 16, from which the images are transmitted to the computer 54 via the cable 36 connecting the cameras, through the analog-to-digital converter 35 and the multiplexer 33. After the end of the test cycle, the internal space 2 is sterilized with a ultraviolet burner 31 with a fan, controlled by a cable 49 from the computer 54, through a multiplexer 33 and a D / A converter 42.

The device according to the present invention also makes it possible to conduct extracorporeal culture in freely programmed conditions of gas composition and temperature and under the influence of magnetic field, radio waves, microwaves and mechanical vibrations as well as selected sections of the white light spectrum, which greatly facilitates the selection of cell lines and the creation of an optimum their development. The use of the invention in the option of neoplastic cell culture may enable tests of the sensitivity of various tumor cell lines to cytostatics.

Claims (2)

1. Device for the selective stimulation of growth and diagnostics of microorganisms in materials, characterized by the fact that it consists of mutually cooperating laboratory (A) and control and measurement (B) modules, of which the laboratory module (A) consists of a temperature stabilizing system, in which is a bi-directional heat pump (10) having an internal (9) and an external heat sink (8) from a gas dosing system comprising: an oxygen valve (25) with an oxygen dosing line (26), a carbon dioxide valve (27), an auxiliary valve (29), the image recording system, which includes a TV camera (16), an illuminating device with a light source (24) and a reflector, a transparent top (3) with a Petri plate (32) with the tested material, diffraction a grid (21), an optical condenser (23), a linear drive (22), a rotating disk (19), filters (18) and a disk drive (20), and from a stimulation system including an electromechanical transducer (7), a field coil (5) magnetic ego and electric field electrodes (6). 2. The device according to claim The method of claim 1, characterized in that the control and measurement module (B) includes a multiplexer (33) connected on one side to an analog-to-digital converter (35) transmitting data from sensors: lighting (11), temperature (12), oxygen (13) ), carbon dioxide (14), additional gas (15) and digital-to-analog converter (42) controlling: heat pump (10), electromechanical converter (7), magnetic field coil (5), electric field electrodes (6), linear the drive (22), the stepper drive (20) of the disc (19), the ultraviolet lamp (31), a light source with a reflector (24), an oxygen valve (25), a carbon dioxide valve (27), an additional valve (29), and the other side is connected via link (34) to computer (54).