PL202158B1 - Method for boar semen preservation - Google Patents

Method for boar semen preservation

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Publication number
PL202158B1
PL202158B1 PL363761A PL36376103A PL202158B1 PL 202158 B1 PL202158 B1 PL 202158B1 PL 363761 A PL363761 A PL 363761A PL 36376103 A PL36376103 A PL 36376103A PL 202158 B1 PL202158 B1 PL 202158B1
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PL
Poland
Prior art keywords
semen
dose
boar semen
frozen
glycerol
Prior art date
Application number
PL363761A
Other languages
Polish (pl)
Other versions
PL363761A1 (en
Inventor
Michał Bochenek
Zdzisław Smorąg
Original Assignee
Inst Zootechniki Pa & Nacute S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Inst Zootechniki Pa & Nacute S filed Critical Inst Zootechniki Pa & Nacute S
Priority to PL363761A priority Critical patent/PL202158B1/en
Publication of PL363761A1 publication Critical patent/PL363761A1/en
Publication of PL202158B1 publication Critical patent/PL202158B1/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Sposób polega na rozcieńczeniu dawki nasienia knura rozrzedzalnikiem zawierającym glicerol i zamrożeniu w temperaturze od -10 do -25°C dla badania w okresie do 7 dni, lub w temperaturze -196°C dla badania w dowolnym czasie.The method involves diluting a dose of boar semen with a diluent containing glycerol and freezing it at a temperature of -10 to -25°C for testing for up to 7 days, or at a temperature of -196°C for testing at any time.

Description

Opis wynalazkuDescription of the invention

Przedmiotem wynalazku jest sposób konserwacji nasienia knura do badań laboratoryjnych, przeznaczonego do ustalania jakości materiału reprodukcyjnego i jego zdolności do rozwoju zarodka po zapłodnieniu oraz oceny uszkodzeń chromatyny (DNA).The subject of the invention is a method of preserving boar semen for laboratory tests, designed to determine the quality of reproductive material and its ability to develop the embryo after fertilization, and to assess chromatin (DNA) damage.

Znane metody badania nasienia knura, zwane metodą SCSA, przewidywały badanie jedynie świeżego nasienia, bezpośrednio po pobraniu.Known methods of testing boar semen, called the SCSA method, provided that only fresh semen was tested, immediately after collection.

Celem wynalazku było opracowanie metody konserwacji nasienia knura, pozwalającej na jego przechowywanie bez uszczerbku dla jakości i pozwalająca na przeprowadzenie badania w każdej chwili bez potrzeby ponownego pobierania.The aim of the invention was to develop a method of preserving boar semen which allows for its storage without compromising its quality and allowing the examination to be carried out at any time without the need for re-collection.

Istota wynalazku polega na rozcieńczeniu dawki nasienia knura, zawierającej co najmniej 1 mln plemników, w stosunku 1:1, rozrzedzalnikiem zawierającym 10 do 20% glicerolu, a następnie zamrożeniu.The essence of the invention consists in diluting a dose of boar semen containing at least 1 million sperm in the ratio 1: 1 with a diluent containing 10 to 20% glycerol, and then freezing it.

Tak przygotowaną dawkę zamraża się w temperaturze od -10 do -25°C jeżeli badania chromatyny nastąpią w okresie do 7 dni od pobrania nasienia lub w temperaturze -195°C jeżeli badania nastąpią w dowolnym okresie czasu po zamrożeniu.The prepared dose is frozen at -10 to -25 ° C if chromatin tests take place within 7 days from semen collection or at -195 ° C if tests take place in any period of time after freezing.

Sposób według wynalazku umożliwia gromadzenie i utrwalanie dowolnej ilości próbek nasienia knurów pochodzących z różnych ośrodków i przesyłanie ich w dogodnym momencie do laboratoriów celem badania uszkodzeń chromatyny plemnikowej.The method according to the invention enables the collection and preservation of any number of boar semen samples from various centers and sending them at a convenient time to laboratories for examination of sperm chromatin damage.

Wynalazek przedstawiony jest w przykładzie wykonania w którym konserwacji podlega dawka 2 ml nasienia.The invention is illustrated by an embodiment in which a dose of 2 ml of semen is preserved.

Tę dawkę rozcieńcza się rozrzedzalnikiem zawierającym 15% glicerolu.This dose is diluted with a diluent containing 15% glycerol.

Po rozcieńczeniu całość zamyka się w naczyniu mrożeniowym i zamraża do temperatury -20°C w zamraż alniku lodówki.After dilution, it is closed in a freezing vessel and frozen to -20 ° C in the freezer of a refrigerator.

Po pięciu dniach, wobec potrzeby przeprowadzenia badania, próbkę zakonserwowaną powyższym sposobem, rozmraża się w temperaturze 37°C i poddaje badaniom uszkodzeń chromatyny (DNA).After five days, for testing purposes, a sample preserved as above is thawed at 37 ° C and subjected to chromatin (DNA) damage testing.

Claims (5)

1. Sposób konserwacji nasienia knura, które to nasienie wykorzystywane jest do badania uszkodzeń chromatyny (DNA), znamienny tym, że dawkę nasienia rozcieńcza się w stosunku 1:1 rozrzedzalnikiem z dodatkiem glicerolu, zaś całość zamyka się w naczyniu mrożeniowym i zamraża.1. A method of preserving boar semen, which semen is used to test chromatin (DNA) damage, characterized in that the dose of semen is diluted 1: 1 with a diluent with the addition of glycerol, and the whole is closed in a freezing vessel and frozen. 2. Sposób według zastrz. 1, znamienny tym, że zamrażana dawka nasienia zawiera co najmniej 1 mln sztuk plemników.2. The method according to p. The method of claim 1, characterized in that the frozen dose of semen contains at least 1 million sperm. 3. Sposób według zastrz. 1, znamienny tym, że dodatek glicerolu w rozrzedzalniku stanowi 10 - 20%.3. The method according to p. The process of claim 1, wherein the addition of glycerol in the diluent is 10 - 20%. 4. Sposób według zastrz. 1, znamienny tym, że dawkę nasienia zamraża się w temperaturze od - 10 do - 25°C.4. The method according to p. The method of claim 1, wherein the seed dose is frozen at -10 to -25 ° C. 5. Sposób według zastrz. 1, znamienny tym, że dawkę nasienia zamraża się w temperaturze - 196°C.5. The method according to p. The method of claim 1, characterized in that the seed dose is frozen at -196 ° C.
PL363761A 2003-11-27 2003-11-27 Method for boar semen preservation PL202158B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL363761A PL202158B1 (en) 2003-11-27 2003-11-27 Method for boar semen preservation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL363761A PL202158B1 (en) 2003-11-27 2003-11-27 Method for boar semen preservation

Publications (2)

Publication Number Publication Date
PL363761A1 PL363761A1 (en) 2005-05-30
PL202158B1 true PL202158B1 (en) 2009-06-30

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PL363761A PL202158B1 (en) 2003-11-27 2003-11-27 Method for boar semen preservation

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PL (1) PL202158B1 (en)

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PL363761A1 (en) 2005-05-30

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Effective date: 20121127