PL180272B1 - Biopreparation for ensilaging fodder containing raw starch, especially potatoes and cereals - Google Patents

Abstract

Biopreparation for ensiling feed containing raw starch, especially potatoes and cereals, containing lactic acid bacteria, complex enzymes, starter substances and filler, characterized in that it contains a new one Lactobacillus planlarum K strain with the number of bacteria at least 106 in 1 g of the preparation, glucoamylase with the activity of at least 3500 JGA / g of the preparation and from 0.1% to 1.0% of the starter, most preferably in the form of yeast extract.

Description

The subject of the invention is a biopreparation for ensiling feed containing raw starch, especially potatoes and cereals, intended for pigs and cattle.

The biological preservation of fodder is based on the lactic fermentation process. The resulting lactic acid lowers the pH of the environment and thus inhibits the growth of spoilage microorganisms. Modern biological methods of fodder silage use the additives of biopreparations containing starter cultures of selected strains of lactic acid bacteria, sometimes supplemented with cellulolytic and / or proteolytic and / or amylolytic enzymes. Bacteria contained in dry preparations are usually deposited on fillers that give the preparations the desired structure. Known fillers include, for example, wheat bran, corn grits, bentonite and quartz flour. Moreover, in order to intensify the initial stage of bacterial growth, starter substances are added to the preparations. Known starters include carbohydrates, such as, for example, sucrose, sorbitol, mannitol, glucose and lactose. Amylolytic and cellulolytic enzymes introduced into biopreparations, through partial hydrolysis of structural and storage carbohydrates, make available simple sugars, necessary for the development of lactic acid bacteria starter cultures.

The preparation for silage for feed containing raw starch (trade name Lactamyl), known from the Polish patent application no. g of the preparation and glucoamylase with an activity of at least 5000 JGA / g of the preparation, and starter sugar and filler. This preparation, added to the fodder containing raw potato and cereal starch, causes the ensuing process to degrade its structure, and to partially hydrolyze it, at least to the same extent as thermal treatment, i.e. the steaming of potatoes before ensiling.

The aim of the invention is to develop a biopreparation intended for the ensilage of fodder containing raw potato and cereal starch, which contains amylolytic enzymes in the complex of amylases produced by lactic acid bacteria.

Unexpectedly, it turned out that the use of a properly selected strain of lactic acid bacteria allows for a significant reduction in the addition of amylolytic enzymes to the biopreparation. At the same time, an increased effect of its action on the degree of decomposition and bioavailability of raw potato and cereal starch contained in the silage for monogastric animals was increased.

The biopreparation according to the invention, containing lactic acid bacteria, enzyme complex, starter substances and filler, is characterized by the fact that it contains a new strain of Lactobacillus plantarum K KKP / 593 / p in the amount of at least 10 ° bacteria / g of the preparation, glucoamylase with an activity of at least 3500 JGA / g formulation and 0.1% to 1.0% starter, most preferably yeast extract.

180 272

Yeast extract is a concentrated, free of cell walls content of yeast cells in the form of pastes, liquid or powder. The yeast extract used in the invention is a commercial preparation in the form of a powder, which has so far been used in the food industry - as an additive to canned food and in microbiology - as an ingredient of nutrients. The use of known starter substances in the invention is also permitted, but the yeast extract, containing in addition to carbohydrates, amino acids, peptides and vitamins (especially from group B), is a more complete source of essential nutrients for the development of the Lactobacillus plantarum strain.

JGA is the amount of enzyme which, under the assay conditions (temperature 30 ° C, pH 4.5), during 1 hour releases 1 mg glucose from soluble starch.

The new Lactobacillus plantarum K strain used in the biopreparation according to the invention is deposited in the Collection of Industrial Microbial Cultures of the Institute of Agricultural and Food Biotechnology under the number KKP / 593 / p.

The biopreparation according to the invention, in comparison with the known preparation Lactamyl, with a higher efficiency of action, contains only one strain of lactic acid bacteria with the ability to produce amylolytic enzymes, and thus less added units of glucoamylase activity, which makes it cheaper to produce.

In the process of fodder containing raw starch, the addition of the preparation according to the invention causes that the amount of lactic acid produced from the starch decomposition products contained in the fodder is at a level that guarantees silage of good quality and stability. The amount of released simple sugars shows the progressive decomposition of starch, as shown in Table 1:

Table 1

The effect of the addition of the preparation according to the invention in comparison with the addition of Lactamyl on the silage composition after 6 weeks of ensiling

Biopre- parat Potatoes, content in% Silage pH Content in% * / dry mass starch saccharose dry mass direct sugars ed. acid lactic acid acetic acid butyric 1. 33.4 19 0.4 4.2 32.1 4.1 2.5 0.5 lack 15 2. 33.4 19 0.4 4.2 33.3 4.8 2.5 0.5 lack 25

1.- Lactamyl - 1.5 kg / t of silage,

2. - biopreparation according to the invention - 1.5 kg / t silage, * / - amount of starch broken down into glucose,%.

The invention is illustrative of an exemplary embodiment without limiting its scope.

Example

1 kg of loose biopreparation was made by mixing the ingredients in the following proportions: 10 g of dry concentrate of Lactobacillus plantarum K bacteria with the number of bacteria 5 x 10 ⁸ / g of concentrate, 120 g of glucoamylase with the activity of 30,000 JGA / g of preparation, 100 g of yeast extract and 100 g of glucose and 670 g of ground wheat bran.

The obtained preparation was used to prepare 1 t of silage with the following composition: 750 kg - raw potatoes cut into pieces, 150 kg - cereal middlings, 100 kg - bran, 1 kg - NaCl and 1 kg - biopreparation. The ingredients were mixed, sprinkling several centimeter layers. The silage is thoroughly compacted and covered with silage foil. The pickling process takes 8 weeks. The obtained silage is of high quality and nutritional value, the parameters of which are given in Table 1, intended for pigs and cattle.

180 272

Publishing Department of the UP RP. Mintage 60 copies. Price PLN 2.00.

Claims (1)

Patent claim Biopreparation for ensiling feed containing raw starch, especially potatoes and cereals, containing lactic acid bacteria, enzyme complex, starter substances and filler, characterized by the fact that it contains a new strain of Lactobacillus plantarum K with a bacterial count of at least 10 ° in 1 g of the preparation , a glucoamylase with an activity of at least 3500 JGA / g formulation and 0.1% to 1.0% starter, most preferably in the form of yeast extract.