PL144097B1 - Method of obtaining mutants of increased ability of producing enzymes - Google Patents

Method of obtaining mutants of increased ability of producing enzymes Download PDF

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Publication number
PL144097B1
PL144097B1 PL25255485A PL25255485A PL144097B1 PL 144097 B1 PL144097 B1 PL 144097B1 PL 25255485 A PL25255485 A PL 25255485A PL 25255485 A PL25255485 A PL 25255485A PL 144097 B1 PL144097 B1 PL 144097B1
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PL
Poland
Prior art keywords
increased ability
mutants
producing enzymes
medium
thermostat
Prior art date
Application number
PL25255485A
Other languages
Polish (pl)
Other versions
PL252554A1 (en
Inventor
Marian Tyc
Krystyna Wituch
Barbara Pelka
Jadwiga Zakrzewska
Krystyna Wawrzyniak
Jan Piorunowski
Original Assignee
Tarchominskie Zaklad Farma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Tarchominskie Zaklad Farma filed Critical Tarchominskie Zaklad Farma
Priority to PL25255485A priority Critical patent/PL144097B1/en
Publication of PL252554A1 publication Critical patent/PL252554A1/en
Publication of PL144097B1 publication Critical patent/PL144097B1/en

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  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (1)

1. Zastrzezenie patentowe Sposób otrzymywania mutantów £.coli o zwiekszonej zdolnosci wytwarzania acylazy pe¬ nicylinowej przez dzialanie czynników mutagennych, znamienny tym, ze komórki szcze¬ pu wytwarzajacego acylaze penicylinowa po dzialaniu czynników mutagennych wysiewa sie na pod¬ loze hodowlane, Inkubuje w termostacie, powstale pojedyncze kolonie przeszczepia na podloze agarowe (Christensena) stosowane do okreslania zdolnosci przyswajania cytrynianu, ponownie inkubuje w termostacie 1 na podstawie zabarwienia podloza wydziela sie mutanty niezdolne do wykorzystywania cytrynianu i porównuje ich aktywnosc enzymatyczna w znany sposób metoda hodowli w kolbach na trzesawce z aktywnoscia szczepu wyjsciowego. PL1. Patent claim A method of obtaining E.coli mutants with increased ability to produce penicillin acylase by the action of mutagenic factors, characterized in that the cells of the penicillin acylase producing strain after the action of mutagenic factors are sown on the culture medium, incubated in a thermostat, the resulting single colonies are transplanted onto agar (Christensen) medium used to determine the ability of citrate uptake, re-incubated in a thermostat 1 based on the color of the medium, mutants unable to use citrate are isolated and their enzymatic activity is compared in a known manner by the tricycle culture method with that of the strain output. PL
PL25255485A 1985-03-25 1985-03-25 Method of obtaining mutants of increased ability of producing enzymes PL144097B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL25255485A PL144097B1 (en) 1985-03-25 1985-03-25 Method of obtaining mutants of increased ability of producing enzymes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL25255485A PL144097B1 (en) 1985-03-25 1985-03-25 Method of obtaining mutants of increased ability of producing enzymes

Publications (2)

Publication Number Publication Date
PL252554A1 PL252554A1 (en) 1986-10-07
PL144097B1 true PL144097B1 (en) 1988-04-30

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ID=20025896

Family Applications (1)

Application Number Title Priority Date Filing Date
PL25255485A PL144097B1 (en) 1985-03-25 1985-03-25 Method of obtaining mutants of increased ability of producing enzymes

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PL (1) PL144097B1 (en)

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Publication number Publication date
PL252554A1 (en) 1986-10-07

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