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Application filed by Tarchominskie Zaklad FarmafiledCriticalTarchominskie Zaklad Farma
Priority to PL25255485ApriorityCriticalpatent/PL144097B1/en
Publication of PL252554A1publicationCriticalpatent/PL252554A1/en
Publication of PL144097B1publicationCriticalpatent/PL144097B1/en
Micro-Organisms Or Cultivation Processes Thereof
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Claims (1)
1. Zastrzezenie patentowe Sposób otrzymywania mutantów £.coli o zwiekszonej zdolnosci wytwarzania acylazy pe¬ nicylinowej przez dzialanie czynników mutagennych, znamienny tym, ze komórki szcze¬ pu wytwarzajacego acylaze penicylinowa po dzialaniu czynników mutagennych wysiewa sie na pod¬ loze hodowlane, Inkubuje w termostacie, powstale pojedyncze kolonie przeszczepia na podloze agarowe (Christensena) stosowane do okreslania zdolnosci przyswajania cytrynianu, ponownie inkubuje w termostacie 1 na podstawie zabarwienia podloza wydziela sie mutanty niezdolne do wykorzystywania cytrynianu i porównuje ich aktywnosc enzymatyczna w znany sposób metoda hodowli w kolbach na trzesawce z aktywnoscia szczepu wyjsciowego. PL1. Patent claim A method of obtaining E.coli mutants with increased ability to produce penicillin acylase by the action of mutagenic factors, characterized in that the cells of the penicillin acylase producing strain after the action of mutagenic factors are sown on the culture medium, incubated in a thermostat, the resulting single colonies are transplanted onto agar (Christensen) medium used to determine the ability of citrate uptake, re-incubated in a thermostat 1 based on the color of the medium, mutants unable to use citrate are isolated and their enzymatic activity is compared in a known manner by the tricycle culture method with that of the strain output. PL
PL25255485A1985-03-251985-03-25Method of obtaining mutants of increased ability of producing enzymes
PL144097B1
(en)