PL138325B1 - Process for preparing novel derivatives of 5-or 7-/o-hydroxyphenyl/-2,3-dihydro-1,4-diazepines - Google Patents

Process for preparing novel derivatives of 5-or 7-/o-hydroxyphenyl/-2,3-dihydro-1,4-diazepines

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Publication number
PL138325B1
PL138325B1 PL23688482A PL23688482A PL138325B1 PL 138325 B1 PL138325 B1 PL 138325B1 PL 23688482 A PL23688482 A PL 23688482A PL 23688482 A PL23688482 A PL 23688482A PL 138325 B1 PL138325 B1 PL 138325B1
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parts
dihydro
methyl
hydrogen
hydroxyphenyl
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PL23688482A
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PL236884A1 (en
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Krzysztof Kostka
Malgorzata Owczarek
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Akad Medyczna
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Przedmiotem wynalazku jest nowa metoda syntezy nowych 2,3-dihydro-1,4-diazepin z podstawnikami o-hydroksyfenylowymi w pozycji 5 lub 7 tego ukladu. Pochodne 2,3-dihydro-1,4-diazepin z podstawnikami o-hy- droksyfenylowymi i metody ich syntezy sa nieopisane w pismiennictwie literatury swiatowej.Znane sa natomiast inne ficzne pochodne 2,3-dihydro-1,4-diazepin z róznego typu podstawnikami (np. metyl, etyl, fenyl, grupa karboksylowa) w róznych pozycjach, które otrzymuje sie w wyniku kondensacji odpo¬ wiednich alifatycznych badz aromatycznych 1,2-dwuamin z 0-dwuketonami lub 0-dwualdehydami (B.M.H. Pot¬ ter, M.W. Coleman, A.M. Mohro, J. Heterocycl. Chem. 1975, 12/4/, 611-614, C. Barnett, H.P. Cleghorn, G.E.Gross, D. Lloyd, D.R. Marshall., J. Chem. Soc. Id 1966, 93-95, D. Lloyd, R.H. Mcdougall, D.R. Marshall, J.Chem. Soc. /c/, 1966, 780-782, D. Lloyd, D.R. Marshall, 1956, 2597-2600).Sposobem wedlug wynalazku zwiazki o wzorze 1 i 2, w których podstawnik R oznacza reszte alkilowa szczególnie grupe metylowa, etylowa lub wodór, Ri stanowi grupe karboksylowa, metylowa, etylowa, amidowa lub wodór, R2 i R3 stanowi reszte metylowa, nitrowa lub wodór, mozna otrzymac w wyniku reakcji kondensacji pochodnych benzo-7-pironu (chromonu) z etylenodwuamina i jej pochodnymi, najkorzystniej w metanolu badz etanolu wobec nadmiaru aminy (2- lub 5-krotnego). Kondensacje prowadzi sie w temperaturze pokojowej w cza¬ sie 5—11 dni. W niektórych przypadkach reagenty dodatkowo ogrzewano w temperaturze wrzenia rozpuszczalni¬ ka. Pochodne chromonu w reakcji z ety lenodwuamina tworza odpowiednie 5-/o-hydroksyfenylo/-2,3-dihydro- 1,4-diazepiny o wzorze 2, w którym R stanowi wodór, Ri stanowi grupe karboksylowa, metylowa, etylowa, amidowa, lub wodór, R2 i R3 stanowi reszte metylowa, nitrowa lub wodór. W reakcji z N-alkiloetylodwuamina powstaja dwa szeregi diazepin z izomerycznej strukturze: pochodne N-alkilo-5-/o-hydroksyfenylo/-2,3-dihydro-1- ,4-diazepiny przedstawione wzorem 2, w którym podstawniki omówiono powyzej oraz pochodne N-alkilo-7-/o- hydroksy-fenylo/- 2,3-dihydro-1,4-diazepiny przedstawione wzorem 1, w którym R stanowi reszte alkilowa szcze¬ gólnie grupe metylowa, etylowa, lub wodór, Ht stanowi grupe karboksylowa, metylowa, etylowa, amidowa lub wodór, R2 i R3 stanowi reszte metylowa, nitrowa lub wodór.Stwierdzono, ze badane zwiazki a otrzymane sposobem wedlug wynalazku wykazuja depresyjny wplyw na OUN (dzialanie uspokajajace).[Dzialaja antagonistycznie w stosunku do amfetaminy i apomorfiny. Zwiazki te wstrzykniete w dawkach toksycznych wywoluja u myszy zmniejszenie ruchliwosci spontanicznej, zaburzenia2 138 325 koordynacji ruchów oraz przedluzenie snu heksobar bita I owego i zmniejszenie ruchliwosci amfetaminowej. Nie¬ które zwiazki dzialaja spazmolitycznie na wyosobnione jelito swinki morskiej. LD50 badanych zwiazków wynosi od powyzej 2,0 g/kg do 0,2 g/kg.Ponizej podano przyklady wykonania sposobu wedlug wynalazku, w których czesci wagowe oznaczaja gramy a czesci objetosciowe mililitry.Przyklad I. W kolbie okraglodennej umieszczono 1,9 czesci wagowych (0,01 mola) kwasu 2-chromo- nokarboksylowego, 3,0 czesci wagowe (0,05 mola) etylenodwuaminy i 180 czesci objetosciowych metanolu.Reagenty co kilka dni ogrzewano w temperaturze 50°C. Po 11 dniach oddestylowano rozpuszczalnik i czesciowo nadmiar aminy. Oleista pozostalosc suszono do zaniku zapachu aminy a nastepnie rozpuszczono w 20 czesciach objetosciowych wody. Roztwór doprowadzono do pH okolo 7 kwasem solnym. Produkt odsaczono i przekrysta- lizowano z wody otrzymujac 2,10 czesci wagowych zwiazku o wzorze 2, w którym Ri oznacza grupe karboksy¬ lowa, R - R2 = R3 jest wodorem. Temperatura topnienia 293°C z rozkladem. Wydajnosc reakcji 90,5%.Postepujac sposobem opisanym w przykladzie I, uzywajac odpowiednich kwasów 2-chromonokarboksylo- wych i etylenodwuaminy otrzymuje sie nowe zwiazki o wzorze 2 przedstawionym na rysunku, których to pod¬ stawniki i temperatutrze topnienia otrzymanych zwiazków przedstawiono w tabeli I.Tabela I.[ Przyklad T II III IV ¦ R Rj R2 R3 H COOH CH3 H H COOH H CH3 H COOH N02 H ' Temp. top. °c 287 rozklad 276 rozklad 293 rozklad Wydajnosc w% 95,5 I 97,6 97,8 Przyklad V. W kolbie umieszczono 3,8 czesci wagowe (0,02 mola) kwasu 2-chromonokarboksy- lowego i 15 czesci objetosciowych bezwodnego etanolu a nastepnie dodano 2,96 czesci wagowe (0,04 mola) N-metyloetylenodwuaminy rozpuszczonej w 5 czesciach objetosciowych bezwodnego etanolu.Po 5 dniach reagenty wstawiono do chlodni na dobe. Otrzymano 0,53 czesci wagowe mieszaniny produ¬ któw, która ogrzano do wrzenia z 10 czesciami objetosciowymi wody. Osad nierozpuszczalny odsaczono otrzymano 0,30 czesci wagowe zwiazku o temperaturze topnienia 160—162° (otrzymana mieszanine mozna rozdzielic stosujac chloroform). Z przesaczu etanolowego po oddestylowaniu etanolu i dodaniu wody wyizolowa¬ no dodatkowo 0,23 czesci wagowe zwiazku o temperaturze topnienia 160—162°C. Lacznie otrzymano 0,53 czesci wagowe (krystalizacja z metanolu) zwiazku o wzorze ogólnym 1, w którym R oznacza grupe metylowa,Ri oznacza grupe karboksylowa, R2 = R3 oznacza wodór. Wydajnosc 10,7%. Przesacz wodny zadawano stopniowo kwasem solnym doprowadzajac do ph okolo 7. Wypadajacy osad odsaczono, przemyto eterem etylowym. Otrzy¬ mano 1,94 czesci wagowe zwiazku o wzorze ogólnym 2, w którym R oznacza grupe metylowa, Rt oznacza grupe karboksylowa, R2 = R3 jest wodorem. Temperatura topnienia 244°C z rozkladem — krystalizacja z wody. Wy¬ dajnosc 39,4%. Postepujac sposobem opisanym w przykladzie V, uzywajac odpowiednich kwasów 2-chromono- karboksy Iowych i N-metyloetylenodwuaminy otrzymuje sie nowe zwiazki o wzorach przedstawionych na rysun¬ ku, których to podstawniki i temperaturetopnienia przedstawiono w tabeli II.Tabela II.Przyklad VI VII VIII wzór 2 i 2 < 2 R CH3 CH3 CH3 CH3 CH3 Ri * R2 COOH CH3 COOH CH3 COOH H COOH H COOH N02 R3 H H CH3 CH3 H Temp. tpp.°C 253 rozklad 186-188 248 rozklad 204-206 i 289 rozklad Wydajnosc w% 42,3 11,5 28,8 13,4 55,2 Przyklad IX.W kolumnie umieszczono 6,0 czesci wagowych (0,1 mola) etylenodwuaminy rozpuszczo¬ nej w 180 czesciach objetosciowych metanolu. Do roztworu wkraplano w ciagu 0,5 godziny metanolowy roztwór (60 czesci objetosciowych) 2,92 czesci wagowe (0,02 mola) chromonu. Po 5 dniach reagenty ogrzewano w tem¬ peraturze wrzenia rozpuszczalnika w ciagu 5 godzin. Oddestylowano rozpuszczalnik i czesciowo nadmiar aminy.138325 3 Oleista pozostalosc suszono do zaniku zapachu aminy. Dodano 25 czesci objetosciowych metanolu, wstawiono do chlodni. Osad odsaczono (inne produkty reakcji), przesacz zatezono do sucha i ponownie suszono a nastepnie przemywano cieplym benzenem (ok. 50 czesci objetosciowych) do chwili, w której mazisty osad calkowicie rozpuszcza sie w wodzie. Produkt przekrystalizowano z wody, otrzymujac 1,20 czesci wagowych zwiazku o wzo¬ rze ogólnym 2, w którym R =Ri = R2 = R3 oznacza wodór. Temperatura topnienia 204-206°C, wydajnosc 32%. Postepujac sposobem opisanym w przykladzie IX, uzywajac odpowiednich chromonów i etylenodwuaminy otrzymuje sie zwiazki o wzorze 2, w którym podstawniki i temperatury topnienia przedstawiono w ponizszej tabeli III.Przyklad X XI XII XIII R H H H H Ri H CH3 C2H5 CONHC/CH3/3 Tabe R2 CH3 H H H a III.R3 H H H H Temp. top.°C 191-192,5 227-229 210-212 248 rozklad Wydajnosc w% 48,6 30,3 20,8 49,0 Zastrzezenie patentowe Sposób wytwarzania nowych pochodnych 5-lub 7-o-hydroksyfenylo-2,3-dihydro-1,4-diazepin o wzorach ogólnych 1 i 2, w których R stanowi wodór lub podstawnik alkilowy, R! stanowi grupe karboksylowa, metylo¬ wa, etylowa, amidowa, lub wodór, R2 i R3 stanowi grupe metylowa, nitrowa lub wodór, znamienny tym, ze zwiazki powyzsze otrzymuje sie w reakcji kondensacji benzo-7-pironu z etylenodwuamina i jej pochod¬ nymi, korzystnie w srodowisku rozpuszczalników organicznych wobec nadmiaru wody.CM£CM2 WZ<£R 4 YiZÓk 2 PLThe subject of the invention is a new method of synthesizing new 2,3-dihydro-1,4-diazepines with o-hydroxyphenyl substituents in the 5 or 7 position of this system. 2,3-dihydro-1,4-diazepine derivatives with o-hydroxyphenyl substituents and the methods of their synthesis are not described in the literature of the world literature, but other phytic derivatives of 2,3-dihydro-1,4-diazepines of various types are known substituents (e.g. methyl, ethyl, phenyl, carboxyl) at various positions, which are obtained by condensation of suitable aliphatic or aromatic 1,2-diamines with O-dicetones or O-dialdehydes (BMH Potter, MW Coleman , AM Mohro, J. Heterocycl. Chem. 1975, 12 (4), 611-614, C. Barnett, HP Cleghorn, GEGross, D. Lloyd, DR Marshall., J. Chem. Soc. Id 1966, 93- 95, D. Lloyd, R. H. Mcdougall, DR Marshall, J. Chem. Soc. (1966, 780-782, D. Lloyd, DR Marshall, 1956, 2597-2600). The compounds of formula I and 2, in which R is an alkyl residue, especially a methyl, ethyl or hydrogen group, Ri is a carboxyl, methyl, ethyl, amide or hydrogen group, R2 and R3 is a methyl, nitro or hydrogen residue hydrogen, can be obtained by condensation of benzo-7-pyrone (chromone) derivatives with ethylene diamine and its derivatives, most preferably in methanol or ethanol in the presence of an excess of amine (2 or 5 times). The condensations are carried out at room temperature for 5 to 11 days. In some cases, the reactants were additionally heated to the reflux temperature of the solvent. Chromone derivatives by reaction with ethylenediamine form the corresponding 5- (o-hydroxyphenyl) -2,3-dihydro-1,4-diazepines of the formula II, where R is hydrogen, R 1 is carboxyl, methyl, ethyl, amide, or hydrogen, R2 and R3 are methyl, nitro or hydrogen residues. In the reaction with N-alkylethyldiamine, two series of diazepines with an isomeric structure are formed: N-alkyl-5- (o-hydroxyphenyl) -2,3-dihydro-1-, 4-diazepine derivatives represented by the formula 2, in which the substituents are discussed above, and derivatives N-alkyl-7- (o-hydroxy-phenyl) -2,3-dihydro-1,4-diazepines represented by formula I, wherein R is an alkyl residue, especially a methyl, ethyl or hydrogen group, Ht is a carboxyl group , methyl, ethyl, amide or hydrogen, R2 and R3 represent a methyl, nitro or hydrogen residue. It has been found that the tested compounds a obtained by the method of the invention have CNS depressant (sedative) effects. [They antagonize amphetamine and apomorphine. These compounds, injected at toxic doses, induce in mice a reduction in spontaneous mobility, impaired coordination of movements, and prolonged sleep of the hexobar and a reduction in amphetamine mobility. Some compounds have a spasmolytic effect on the isolated guinea pig intestine. The LD50 of the compounds tested is from above 2.0 g / kg to 0.2 g / kg. Below are examples of the method according to the invention in which the parts by weight are grams and the parts by volume are milliliters. Example I. 1.9 parts are placed in a round bottom flask. 2-chromonecarboxylic acid (0.01 mole), 3.0 parts by weight (0.05 mole) of ethylenediamine and 180 parts by volume of methanol. The reactants were heated to 50 ° C every few days. After 11 days, the solvent and partly the excess amine were distilled off. The oily residue is dried until the amine odor disappears and then dissolved in 20 parts by volume of water. The solution was brought to a pH of about 7 with hydrochloric acid. The product was filtered off and recrystallized from water to give 2.10 parts by weight of the compound of formula II in which R 1 is a carboxyl group, R - R 2 = R 3 is hydrogen. Melting point 293 ° C with decomposition. The yield of the reaction is 90.5%. Following the procedure described in Example 1, using the appropriate 2-chromonecarboxylic acids and ethylenediamine, new compounds of the formula II shown in the figure are obtained, the substituents of which and the melting points of the compounds obtained are shown in Table I. Table I. [Example T II III IV ¦ R Rj R2 R3 H COOH CH3 HH COOH H CH3 H COOH NO2 H 'Temp. top. ° c 287 decomposition 276 decomposition 293 decomposition Yield in% 95.5 I 97.6 97.8 Example V. 3.8 parts by weight (0.02 mole) of 2-chromonecarboxylic acid and 15 parts by volume of anhydrous ethanol were placed in the flask and then 2.96 parts by weight (0.04 mol) of N-methyl ethylenediamine dissolved in 5 parts by volume of anhydrous ethanol were added. After 5 days, the reagents were placed in the cooler overnight. 0.53 parts by weight of a mixture of products were obtained which was boiled with 10 parts by volume of water. The insoluble precipitate was filtered off, giving 0.30 parts by weight of a compound with a melting point of 160 ° -162 ° (the resulting mixture can be separated by using chloroform). An additional 0.23 parts by weight of a compound having a melting point of 160 ° -162 ° C. was isolated from the ethanol filtrate after distilling off the ethanol and adding water. A total of 0.53 parts by weight (crystallization from methanol) of the compound of the general formula I in which R is a methyl group, Ri is a carboxyl group, R2 = R3 is hydrogen was obtained. Yield 10.7%. The aqueous filtrate was gradually added with hydrochloric acid, adjusting the pH to about 7. The precipitate that formed was filtered off, washed with diethyl ether. There were obtained 1.94 parts by weight of the compound of general formula II in which R is methyl, Rt is carboxyl, R2 = R3 is hydrogen. Melting point 244 ° C with decomposition - crystallization from water. Yield 39.4%. Following the procedure described in example V, using the appropriate 2-chromonocarboxylic acids and N-methyl ethylenediamine, new compounds are obtained with the formulas shown in the figure, the substituents and the melting point of which are shown in Table II. Table II. Example VI VII VIII formula 2 and 2 <2 R CH3 CH3 CH3 CH3 CH3 Ri * R2 COOH CH3 COOH CH3 COOH H COOH H COOH NO2 R3 HH CH3 CH3 H Temp. tpp. ° C 253 decomposition 186-188 248 decomposition 204-206 and 289 decomposition Yield in% 42.3 11.5 28.8 13.4 55.2 Example IX In the column 6.0 parts by weight (0.1 mole) of ethylenediamine dissolved in 180 parts by volume of methanol. A methanolic solution (60 parts by volume) of 2.92 parts by weight (0.02 mol) of chromone was added dropwise to the solution over 0.5 hours. After 5 days, the reactants were heated to the reflux temperature of the solvent for 5 hours. The solvent was distilled off and some of the excess amine was distilled off. 138323 The oily residue was dried until the amine odor disappeared. 25 volumetric parts of methanol were added, put into cold room. The precipitate was filtered off (other reaction products), the filtrate was concentrated to dryness and dried again, then washed with warm benzene (approx. 50 parts by volume) until the greasy precipitate was completely dissolved in the water. The product was recrystallized from water to give 1.20 parts by weight of a compound of general formula 2 in which R = Ri = R2 = R3 is hydrogen. Melting point 204-206 ° C, 32% yield. By following the procedure described in example IX, the compounds of formula 2 are obtained using the appropriate chromones and ethylenediamine, in which the substituents and melting points are shown in the following table III. a III.R3 HHHH Temp. top ° C 191-192.5 227-229 210-212 248 decomposition Yield in% 48.6 30.3 20.8 49.0 Patent claim Process for the preparation of new 5-or 7-o-hydroxyphenyl-2,3 derivatives -dihydro-1,4-diazepines of general formulas 1 and 2 in which R is hydrogen or an alkyl substituent, R < is a carboxyl, methyl, ethyl, amide or hydrogen group, R2 and R3 is a methyl, nitro or hydrogen group, characterized in that the above compounds are obtained by the condensation of benzo-7-pyrone with ethylenediamine and its derivatives, preferably in the environment of organic solvents in the presence of excess water CM £ CM2 WZ <£ R 4 YiZÓk 2 PL

Claims (1)

1. Zastrzezenie patentowe Sposób wytwarzania nowych pochodnych 5-lub 7-o-hydroksyfenylo-2,3-dihydro-1,4-diazepin o wzorach ogólnych 1 i 2, w których R stanowi wodór lub podstawnik alkilowy, R! stanowi grupe karboksylowa, metylo¬ wa, etylowa, amidowa, lub wodór, R2 i R3 stanowi grupe metylowa, nitrowa lub wodór, znamienny tym, ze zwiazki powyzsze otrzymuje sie w reakcji kondensacji benzo-7-pironu z etylenodwuamina i jej pochod¬ nymi, korzystnie w srodowisku rozpuszczalników organicznych wobec nadmiaru wody. CM£CM2 WZ<£R 4 YiZÓk 2 PLClaim 1. A process for the preparation of new 5-or 7-o-hydroxyphenyl-2,3-dihydro-1,4-diazepine derivatives of general formulas 1 and 2, in which R is hydrogen or an alkyl substituent, R! is a carboxyl, methyl, ethyl, amide or hydrogen group, R2 and R3 is a methyl, nitro or hydrogen group, characterized in that the above compounds are obtained by the condensation of benzo-7-pyrone with ethylenediamine and its derivatives, preferably in an environment of organic solvents in the presence of excess water. CM £ CM2 WZ <£ R 4 YiZÓk 2 PL
PL23688482A 1982-06-11 1982-06-11 Process for preparing novel derivatives of 5-or 7-/o-hydroxyphenyl/-2,3-dihydro-1,4-diazepines PL138325B1 (en)

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