PL118433B1 - Process for preparing 2,5-diketogluconic acid - Google Patents
Process for preparing 2,5-diketogluconic acid Download PDFInfo
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- PL118433B1 PL118433B1 PL1978210683A PL21068378A PL118433B1 PL 118433 B1 PL118433 B1 PL 118433B1 PL 1978210683 A PL1978210683 A PL 1978210683A PL 21068378 A PL21068378 A PL 21068378A PL 118433 B1 PL118433 B1 PL 118433B1
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- acid
- glucose
- acetobacter
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- 238000004519 manufacturing process Methods 0.000 title description 5
- RXMWXENJQAINCC-DMTCNVIQSA-N 2,5-didehydro-D-gluconic acid Chemical compound OCC(=O)[C@@H](O)[C@H](O)C(=O)C(O)=O RXMWXENJQAINCC-DMTCNVIQSA-N 0.000 title 1
- 239000002253 acid Substances 0.000 claims description 29
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 21
- 239000008103 glucose Substances 0.000 claims description 21
- 241000589220 Acetobacter Species 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 12
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 7
- 229910052757 nitrogen Inorganic materials 0.000 claims description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical class NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 4
- 239000004202 carbamide Substances 0.000 claims description 4
- 235000001968 nicotinic acid Nutrition 0.000 claims description 4
- 239000011664 nicotinic acid Substances 0.000 claims description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 3
- 150000003863 ammonium salts Chemical class 0.000 claims description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 3
- 239000011707 mineral Chemical class 0.000 claims description 3
- 229960003512 nicotinic acid Drugs 0.000 claims description 3
- 229910052698 phosphorus Inorganic materials 0.000 claims description 3
- 239000011574 phosphorus Substances 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 description 11
- 230000004151 fermentation Effects 0.000 description 11
- 239000002609 medium Substances 0.000 description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- ZWEHNKRNPOVVGH-UHFFFAOYSA-N 2-Butanone Chemical compound CCC(C)=O ZWEHNKRNPOVVGH-UHFFFAOYSA-N 0.000 description 3
- 235000010323 ascorbic acid Nutrition 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 241000032681 Gluconacetobacter Species 0.000 description 2
- 241000209149 Zea Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 239000001058 brown pigment Substances 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 235000010350 erythorbic acid Nutrition 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 150000004715 keto acids Chemical class 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 239000004254 Ammonium phosphate Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 241001600170 Fragum Species 0.000 description 1
- 241001621835 Frateuria aurantia Species 0.000 description 1
- 241001518248 Gluconobacter cerinus Species 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241001587025 Pseudomonas sesami Species 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- DGOBMKYRQHEFGQ-UHFFFAOYSA-L acid green 5 Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 DGOBMKYRQHEFGQ-UHFFFAOYSA-L 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229910000148 ammonium phosphate Inorganic materials 0.000 description 1
- 235000019289 ammonium phosphates Nutrition 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- FLPNUCBSFACIHU-UHFFFAOYSA-N benzene-1,2-diamine ethanol Chemical compound C(C)O.NC1=C(C=CC=C1)N FLPNUCBSFACIHU-UHFFFAOYSA-N 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- FPVGTPBMTFTMRT-UHFFFAOYSA-L disodium;2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate Chemical compound [Na+].[Na+].C1=C(S([O-])(=O)=O)C(N)=CC=C1N=NC1=CC=C(S([O-])(=O)=O)C=C1 FPVGTPBMTFTMRT-UHFFFAOYSA-L 0.000 description 1
- 235000019233 fast yellow AB Nutrition 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 238000004816 paper chromatography Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000013587 production medium Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/58—Aldonic, ketoaldonic or saccharic acids
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Description
Przedmiotem wynalazku jest sposób wytwarzania kwasu 2,5-dwuketoglikonowego.Kwas 2,5-dwuketoglikonowy jest uzytecznym pólpro¬ duktem w syntezie witaminy C. Z tego wzgledu wytwarza sie go przy pomocy wielu róznych rodzajów bakterii takich 5 jak Acetobacter melanogenum, Acetobacter aurantium, Gluconoacetobacter rubiginosus, Gluconoacetobacter li- auifaciens i Pseudomonas sesami. Jednakze zastosowanie tych drobnoustrojów jest niepozadane z przemyslowego punktu widzenia z powodu powstawania duzych ilosci 10 brunatnych lub zólto-brunatnych pigmentów jako produk¬ tów ubocznych hodowli przez co zmniejsza sie czystosc wytwarzanego kwasu 2,5-dwuketoglikonowego.W opisie patentowym St. Zjedn. Amer. nr 3 790 444 ujawniono sposób wytwarzania kwasu. 2,5-dwuketogliko- 15 nowego bez tworzenia sie towarzyszacych brunatnych pigmentów przez nowy szczep oznaczony jako Acetobacter fragum w hodowli zawierajacej jako pozywke glikoze oraz substancje bedaca zródlem azotu. W sposobie tym stosuje sie poczatkowe pH 5,8 i po 32 godzinach otrzymuje kwas 20 2,5-dwuketoglikonowy z wydajnoscia 87% w przeliczeniu na glikoze.Wynalazek dotyczy ekonomicznego procesu wytwarzania kwasu 2,5-dwuketoglikonowego przy zastosowaniu latwo dostepnego szczepu Acetobacter cerinus. Korzystnymi 25 z tych szczepftw sa dwa: IFO 3263 i 3266, które wytwarzaja kwas 2,5-dwuketoglikonowy z wydajnoscia ponad 95% (w przeliczeniu na glikoze).Kwas 2,5-dwuketoglikonowy jest stosowany jako pól¬ produkt przy wytwarzaniu kwasu askorbinowego. Wodny 30 roztwór kwasu 2,5-dwuketoglikonowego mozna selektywnie redukowac w celu uzyskania mieszaniny 2-ketogulonianu i 2-ketoglikonianu, które mozna przeprowadzic w kwasy askorbinowy i erytorbowy.Sposobem wedlug wynalazku kwas 2,5-dwuketoglikono¬ wy otrzymuje sie latwo przez dzialanie bakterii na glikoze, stosujac znany, latwo dostepny szczep Acetobacter cerinus.Przebadano wszystkie dostepne powszechnie szczepy Acetobacter cerinus. W badaniach tych wydajnosc otrzy¬ mywanych ketokwasów wynosila 50—95 % (w przeliczeniu na glikoze). Gdy stosuje sie Acetobacter cerinus korzystnie IFO 3263 lub 3266, wytwarzanymketokwasem jest wylacznie pozadany kwas 2,5-dwuketoglikonowy a proces przebiega z wydajnoscia ponad 95 % (w przeliczeniu na glikoze).Dostepnymi powszechnie szczepami Acetobacter cerinus sa: IFO 3262 (ATCC 12303), 3263, 3264, 3265, 3266, 3267, 3268, 3269.. Szczepy te sa znane i zarejestrowane w katalogu ATCC, wyd, 11, 1974jako ATCC 12303 oraz w rejestrze prowadzo¬ nym przez Institute for Fermentation w Osace jako Gluco- nobacter cerinus IFO 3262 — IFO 3270 (róznica w nazwie szczepu wynika z odmiennej nomenklatury stosowanej przez Instytut w Osace).Wspomniane szczepy Acetobacter cerinus hoduje sie w srodowisku, w którym glówne zródlo wegla stanowi glikoza. Drobnoustroje te nie wymagaja kosztownych zródel azotu takich jak pepton lub ekstrakt miesny.Zwykle hodowle prowadzi sie na pozywce zawierajacej 2,5—20 % glikozy oraz tanie zródlo azotu takie jak mocznik i nieorganiczne zródla azotu takie jak sole amonowe, 118 433118 433 zwlaszcza siarczan amonu, azotan amonu lub fosforan amo¬ nu, a jako wskaznik wzrostu do pozywki dodaje sie kwas nikotynowy. Pozywka zawiera takze sole mineralne, oraz zródlo fosforu.Stezenie glikozy w srodowisku zmienia sie w granicach 2,5 — 20 % korzystnie 10—12 % w celu najbardziej ekono¬ micznego otrzymania kwasu 2,5-dwuketoglikonowego.Temperatura fermentacji wynosi 20—35 °C. korzystnie 25—30°C a zwlaszcza okolo 28 °C. Poczatkowe pH srodo¬ wiska hodowli wynosi 3,5—7,5, korzystnie 5—6. Podczas przebiegu fermentacji wartosc pH utrzymuje sie na pozio- •mie okolo, 5,5 przez dodawanie roztworu wodorotlenku sodu.' Do regulowania wartosci pH mozna tez stosowac weglan wapnia. Dodaje sie go, do hodowli po sterylizacji para pod zwiekszonym cisnieniem w ilosci okolo 30 g na 110 g glikozy.Po inokulacji, srodowisko fermentacyjne miesza sie przy pomocy mieszadla mechanicznego o szybkosci 1700 obro¬ tów/min i napowietrza z szybkoscia 0,5—1 objetosci po¬ wietrza (objetosc brzeczki) minute..Stosujac Acetobacfer cerinus IfO 3263 lub 3266 fermen¬ tacje prowadzi sie az do uzyskania wydajnosci kwasu 2,5-dwuketoglikonowego co najmniej 90 % (w przeliczeniu na glikoze) (36—40 godzin).Za pomoca chromatografii bibulowej stwierdzono, ze konwersja glikozy do lcwasu 2,5-dwuketoglikonowego przebiega w nastepujacy sposób: glikoza^*" kwas 2-ketoglikonowy -£ kwas 2,5-dwuketogIi- konowy glikoza - kwas 5-ketoglikonowjr -+ kwas 2,5 -dwuketo- glikonowy.Zastosowano bibule Whatman nr 1 i nr 4 i uklad roz¬ puszczalników keton metyloetylowy:aceton:kwas mrów¬ kowy:woda (80:6:2:12). Plamki kwasu identyfikowano przez spryskiwanie 0,2 % roztworem etanolowym o-fenyle- nodwuaminy zawierajacym 1 % kwasu azotowego i ogrzewa¬ nie do temperatury okolo 70 °C (kwas 5-ketoglikonowy- niebieski; kwas 2-ketoglikonowy — zólty; kwas 2,5-dwu- ketoglikonowy — zielony).W celach identyfikacyjnych mozna stosowac równiez ciekla chromatografie wysokocisnieniowa.Kwas 2,5-dwuketoglikonowy mozna oddzielic i odzyskac z koncowej brzeczki fermentacyjnej wjakikolwiekkonwencjo¬ nalny, znany sposób. Odsaczona brzeczke fermentacyjna mozna potraktowac borowodorkiem a otrzymana miesza¬ nine kwasów 2-ketoglikonowego i 2-ketogulonowego poddac hydrolizie, uzyskujac,kwasy askorbinowy i erytorbowy.Przyklad I. Przygotowano nastepujace wodne sro¬ dowisko inoculum.; Skladnik g/litr Glikoza, 25 Roztwór namoczonej kukurydzy 5 KH2PO4 0,5 K2HP04 0,5 MgS04 —7H20 0,2 CaCOs ' '" • 6,3 pH 6,2 Wytrzasana kolbe zawierajaca 1 litr srodowiska poddaje sie sterylizacji pare pod zwiekszonym cisnieniem w ciagu 30 minut w temperaturze 121°C. Wartosc pH oziebionego srodowiska wynosi 5,0. Do kolby dodaje sie komórki Aceto- 5 bacter cerinus IFO 3263 pochodzace ze skosu' agarowego jako pozywki (5 ml z 20 ml sterylnej wodnej zawiesiny) i nastepnie kolbe wytrzasa sie na wytrzasarce obrotowej w temperaturze 28 °C w ciagu 24 godzin.Przyklad II. Czesc hodowli kultury dostateczna 10 do wytworzenia 5%. objetosc/objetosc inoculum dodaje sie do 4-litrowego fermentatora z mieszadlem, zawierajacego 2-litry nastepujacej pozywki produkcyjnej: Skladnik g/litr Glikoza 110 15 Roztwór namoczonej kukurydzy 0,5 (NH4)2HP04 0,58 KH2P04 1,5 MgS04-7H20 0,5 mocznik 0,5 20 CuS04-5H20 1 mg _ kwasnikotynowy 300 r pH 6,0 Fermentacje przeprowadza sie w temperaturze okolo 28 °C, przy mieszaniu z szybkoscia 1700 obrotów/jninute 25 i napowietrzaniu z szybkoscia 0,75 objetosc/objetosc brzeczki/minute. Po okresie fermentacji trwajacym okolo 20 godzin, dodaje sie sterylnej glikozy (55 g/litr). Wartosc pH utrzymuje sie na poziomie 5,5 przez dodanie roztworu wodorotlenku sodu. Fermentacje prowadzi sie az do uzys- 30 kania wydajnosci kwasu 2,5-dwuketoglikonowego powyzej 95% (w przeliczeniu na glikoze). Otrzymany produkt wydziela sie, poddajac surowa brzeczke fermentacyjna chromatografii.Postepujac jak opisano wyzej, lecz stosujac Acetobacter 35 cerinus IFO 3266, otrzymuje sie kwas 2,5-dwuketogliko¬ nowy z wydajnoscia powyzej 95 %. (w przeliczeniu na gliko¬ ze). -40 Zastrzezenia patentowe 1. Sposób wytwarzania kwasu 2,5-dwuketoglikonowego w hodowli Acetobacter prowadzonej w warunkach aerobo- 45 wych, znamienny tym, ze prowadzi sie hodowle Aceto¬ bacter cerinus, w temperaturze 20—35 °C i poczatkowym pH 3,5—7,5 na pozywce zawierajacej 2,5—20% glikozy i zródlo azotu, zwlaszcza mocznik lub sole amonowe, sole mineralne i zródlo fosforu, w obecnosci kwasu nikotyno- 50 wego, a nastepnie wyosabnia sie zadany kwas w znany sposób. 2. Sposób wedlug zastrz. 1, znamienny tym, ze hodowle prowadzi sie w temperaturze 25—30 °C, przy poczatkowym pH5—6. 55 3. Sposób wedlug zastrz. 1, znamienny tym, ze jako Acetobacter cerinus stosuje sie szczep IFO 3263. 4. Sposób wedlug zastrz. 1, znamienny tym, ze jako Acetobacter cerinus stosuje sie szczep IFO 3266.LDD Z-d 2, z. 1075/1400/82, n. 95+20 egz.Cena 100 zl PL PL PL The subject of the invention is a method for producing 2,5-diaketoglyconic acid. 2,5-Diketoglyconic acid is a useful intermediate in the synthesis of vitamin C. Therefore, it is produced using many different types of bacteria such as Acetobacter melanogenum, Acetobacter aurantium, Gluconoacetobacter rubiginosus, Gluconoacetobacter liauifaciens and Pseudomonas sesami. However, the use of these microorganisms is undesirable from an industrial point of view due to the formation of large amounts of brown or yellow-brown pigments as culture by-products, thereby reducing the purity of the produced 2,5-diaketoglyconic acid. United American No. 3,790,444 discloses a method for preparing the acid. 2,5-Diketoglycolyte without the formation of associated brown pigments by a new strain designated as Acetobacter fragum in a culture containing glucose as a medium and a nitrogen source substance. This method uses an initial pH of 5.8 and after 32 hours 2,5-diaketoglyconic acid is obtained with a yield of 87% based on glucose. The invention concerns an economic process for the production of 2,5-diaketoglyconic acid using an easily available strain of Acetobacter cerinus. The preferred 25 of these strains are two: IFO 3263 and 3266, which produce 2,5-diaketoglyconic acid with an efficiency of over 95% (based on glucose). 2,5-Diketoglyconic acid is used as an intermediate in the production of ascorbic acid. An aqueous solution of 2,5-diketoglyconic acid can be selectively reduced to obtain a mixture of 2-ketogulonate and 2-ketoglyconate, which can be converted into ascorbic and erythorbic acids. In the method of the invention, 2,5-diketoglyconic acid is easily obtained by the action of bacteria on glucose, using a well-known, easily available strain of Acetobacter cerinus. All commonly available strains of Acetobacter cerinus were tested. In these studies, the yield of the obtained keto acids was 50-95% (calculated as glucose). When Acetobacter cerinus is used, preferably IFO 3263 or 3266, the ketoacid produced is only the desired 2,5-dioketoglyconic acid and the process is performed with an efficiency of over 95% (calculated as glucose). Commonly available strains of Acetobacter cerinus are: IFO 3262 (ATCC 12303), 3263, 3264, 3265, 3266, 3267, 3268, 3269. These strains are known and registered in the ATCC catalog, 11th edition, 1974 as ATCC 12303 and in the register kept by the Institute for Fermentation in Osaka as Gluconobacter cerinus IFO 3262 - IFO 3270 (the difference in the name of the strain results from the different nomenclature used by the Institute in Osaka). The mentioned strains of Acetobacter cerinus are grown in an environment where the main source of carbon is glucose. These microorganisms do not require expensive nitrogen sources such as peptone or meat extract. Typically, cultivation is carried out on a medium containing 2.5-20% glucose and a cheap nitrogen source such as urea and inorganic nitrogen sources such as ammonium salts, especially ammonium sulfate. , ammonium nitrate or ammonium phosphate, and nicotinic acid is added to the medium as a growth indicator. The medium also contains mineral salts and a source of phosphorus. The glucose concentration in the environment ranges from 2.5 to 20%, preferably 10 to 12% in order to obtain 2,5-diaketoglyconic acid in the most economical manner. The fermentation temperature is 20 to 35°C. C preferably 25-30°C and especially about 28°C. The initial pH of the culture medium is 3.5-7.5, preferably 5-6. During fermentation, the pH value is maintained at approximately 5.5 by adding sodium hydroxide solution. Calcium carbonate can also be used to adjust the pH value. It is added to the culture after sterilization by steam under increased pressure in an amount of approximately 30 g per 110 g of glucose. After inoculation, the fermentation medium is mixed using a mechanical mixer at a speed of 1700 rpm and aeration at a rate of 0.5-1 volume of air (volume of wort) per minute. Using Acetobacfer cerinus IfO 3263 or 3266, fermentation is carried out until the yield of 2,5-diaketoglyconic acid is at least 90% (calculated as glucose) (36-40 hours). Using paper chromatography, it was found that the conversion of glucose to 2,5-diaketoglyconic acid takes place as follows: glucose - 2-ketoglyconic acid - 2,5-diketoglyconic acid - glucose - 5-ketoglyconic acid -+ acid 2, 5-Diketoglyconic acid. Whatman No. 1 and No. 4 papers were used and the solvent system was methyl ethyl ketone: acetone: formic acid: water (80: 6: 2: 12). Acid spots were identified by spraying with a 0.2% solution o-phenylenediamine ethanol containing 1% nitric acid and heating to a temperature of about 70°C (5-ketoglyconic acid-blue; 2-ketoglyconic acid - yellow; 2,5-Di-ketoglyconic acid - green). High-pressure liquid chromatography may also be used for identification purposes. 2,5-Diketoglyconic acid may be separated and recovered from the final fermentation broth by any conventional known method. The drained fermentation broth can be treated with borohydride and the resulting mixture of 2-ketoglyconic and 2-ketogulonic acids hydrolyzed to obtain ascorbic and erythorbic acids. Example I. The following aqueous inoculum medium was prepared; Ingredient g/liter Glucose, 25 Soaked corn solution 5 KH2PO4 0.5 K2HP04 0.5 MgS04 —7H20 0.2 CaCOs ' '" • 6.3 pH 6.2 A shaken flask containing 1 liter of medium is sterilized with steam under increased pressure for 30 minutes at 121° C. The pH of the cooled environment is 5.0. Acetobacter cerinus IFO 3263 cells from an agar slant are added to the flask as a medium (5 ml with 20 ml of sterile aqueous suspension) and then the flask is shaken on a rotary shaker at 28°C for 24 hours.Example 2. A portion of the culture sufficient to produce 5% volume/inoculum is added to a 4 liter stirred fermenter containing 2 liters of the following production medium : Ingredient g/liter Glucose 110 15 Soaked corn solution 0.5 (NH4)2HP04 0.58 KH2P04 1.5 MgS04-7H20 0.5 urea 0.5 20 CuS04-5H20 1 mg _ tinic acid 300 r pH 6.0 Fermentations is carried out at a temperature of about 28 °C, with mixing at a speed of 1700 rpm and aeration at a rate of 0.75 volume/volume of wort/minute. After a fermentation period of approximately 20 hours, sterile glucose (55 g/liter) is added. The pH value is maintained at 5.5 by adding sodium hydroxide solution. Fermentation is carried out until the yield of 2,5-diaketoglyconic acid exceeds 95% (calculated as glucose). The product obtained is isolated by subjecting the crude fermentation broth to chromatography. Proceeding as described above, but using Acetobacter cerinus IFO 3266, 2,5-diaketoglyconic acid is obtained with a yield of over 95%. (calculated as glucose). -40 Patent claims 1. A method for producing 2,5-diaketoglyconic acid in an Acetobacter culture carried out under aerobic conditions, characterized in that the Acetobacter cerinus culture is carried out at a temperature of 20-35 °C and an initial pH of 3.5 -7.5 on a medium containing 2.5-20% glucose and a nitrogen source, especially urea or ammonium salts, mineral salts and a phosphorus source, in the presence of nicotinic acid, and then the desired acid is isolated in a known manner. 2. The method according to claim 1, characterized in that the cultivation is carried out at a temperature of 25-30 °C and an initial pH of 5-6. 55 3. The method according to claim 1, characterized in that the Acetobacter cerinus strain is IFO 3263. 4. The method according to claim 1. 1, characterized in that the Acetobacter cerinus strain used is IFO 3266.LDD Z-d 2, no. 1075/1400/82, no. 95+20 copies. Price PLN 100 PL PL PL
Claims (2)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US85295077A | 1977-11-18 | 1977-11-18 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL210683A1 PL210683A1 (en) | 1979-06-18 |
| PL118433B1 true PL118433B1 (en) | 1981-10-31 |
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ID=25314629
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL1978210683A PL118433B1 (en) | 1977-11-18 | 1978-11-03 | Process for preparing 2,5-diketogluconic acid |
Country Status (26)
| Country | Link |
|---|---|
| JP (1) | JPS54145283A (en) |
| AR (1) | AR218348A1 (en) |
| AT (1) | AT363887B (en) |
| AU (1) | AU505434B1 (en) |
| BE (1) | BE872095A (en) |
| BR (1) | BR7807524A (en) |
| CA (1) | CA1119981A (en) |
| CH (1) | CH643592A5 (en) |
| DD (1) | DD140459A5 (en) |
| DE (1) | DE2849393C2 (en) |
| DK (1) | DK152679C (en) |
| ES (1) | ES475216A1 (en) |
| FI (1) | FI782871A (en) |
| FR (1) | FR2409304A1 (en) |
| GB (1) | GB2008116B (en) |
| HU (1) | HU175521B (en) |
| IL (1) | IL55969A0 (en) |
| IT (1) | IT1101715B (en) |
| LU (1) | LU80536A1 (en) |
| NL (1) | NL7811353A (en) |
| NO (1) | NO783877L (en) |
| PL (1) | PL118433B1 (en) |
| PT (1) | PT68789A (en) |
| RO (1) | RO75389A (en) |
| SE (1) | SE7809345L (en) |
| ZA (1) | ZA786487B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4316960A (en) * | 1979-09-28 | 1982-02-23 | Pfizer Inc. | Preparation of 2,5-diketogluconic acid |
| JPS6365970A (en) * | 1987-08-25 | 1988-03-24 | Kyushu Hitachi Maxell Ltd | Motor-driven sprayer |
| FR2820973B1 (en) | 2001-02-19 | 2003-05-23 | Oreal | COMPOSITION COMPRISING VITAMIN C PREPARED DURING APPLICATION, USE OF ENZYMES FOR THE FORMATION OF VITAMIN C FOR TOPICAL USE AND COSMETIC PROCESSING METHOD |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3234105A (en) * | 1962-09-20 | 1966-02-08 | Takeda Chemical Industries Ltd | Method for producing 2-keto-lgulonic acid |
| US3790444A (en) * | 1971-03-09 | 1974-02-05 | Daiichi Seiyaku Co | Process for preparing diketogluconic acid |
| JPS5135485A (en) * | 1974-09-20 | 1976-03-25 | Shionogi Seiyaku Kk | 22 keto ll guronsan no seizohoho |
-
1978
- 1978-09-05 SE SE7809345A patent/SE7809345L/en unknown
- 1978-09-13 AU AU39819/78A patent/AU505434B1/en not_active Expired
- 1978-09-20 FI FI782871A patent/FI782871A/en not_active IP Right Cessation
- 1978-10-31 CH CH1121978A patent/CH643592A5/en not_active IP Right Cessation
- 1978-11-02 HU HU78PI647A patent/HU175521B/en unknown
- 1978-11-03 PL PL1978210683A patent/PL118433B1/en unknown
- 1978-11-03 DD DD78208866A patent/DD140459A5/en unknown
- 1978-11-03 RO RO7895585A patent/RO75389A/en unknown
- 1978-11-14 DE DE2849393A patent/DE2849393C2/en not_active Expired
- 1978-11-15 PT PT68789A patent/PT68789A/en unknown
- 1978-11-16 CA CA000316362A patent/CA1119981A/en not_active Expired
- 1978-11-16 IT IT29866/78A patent/IT1101715B/en active
- 1978-11-16 GB GB7844723A patent/GB2008116B/en not_active Expired
- 1978-11-16 LU LU80536A patent/LU80536A1/en unknown
- 1978-11-16 BR BR7807524A patent/BR7807524A/en unknown
- 1978-11-17 NL NL7811353A patent/NL7811353A/en not_active Application Discontinuation
- 1978-11-17 AT AT0823178A patent/AT363887B/en not_active IP Right Cessation
- 1978-11-17 FR FR7832491A patent/FR2409304A1/en not_active Withdrawn
- 1978-11-17 IL IL55969A patent/IL55969A0/en unknown
- 1978-11-17 NO NO783877A patent/NO783877L/en unknown
- 1978-11-17 ZA ZA00786487A patent/ZA786487B/en unknown
- 1978-11-17 AR AR274472A patent/AR218348A1/en active
- 1978-11-17 JP JP14215078A patent/JPS54145283A/en active Granted
- 1978-11-17 ES ES475216A patent/ES475216A1/en not_active Expired
- 1978-11-17 DK DK512978A patent/DK152679C/en active
- 1978-11-17 BE BE191792A patent/BE872095A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE2849393C2 (en) | 1983-05-05 |
| ES475216A1 (en) | 1979-04-16 |
| SE7809345L (en) | 1979-05-19 |
| BE872095A (en) | 1979-05-17 |
| PT68789A (en) | 1978-12-01 |
| DD140459A5 (en) | 1980-03-05 |
| NL7811353A (en) | 1979-05-22 |
| CH643592A5 (en) | 1984-06-15 |
| FR2409304A1 (en) | 1979-06-15 |
| LU80536A1 (en) | 1980-06-05 |
| NO783877L (en) | 1979-05-21 |
| CA1119981A (en) | 1982-03-16 |
| IT7829866A0 (en) | 1978-11-16 |
| BR7807524A (en) | 1979-07-24 |
| ZA786487B (en) | 1979-10-31 |
| JPS579357B2 (en) | 1982-02-20 |
| RO75389A (en) | 1980-11-30 |
| PL210683A1 (en) | 1979-06-18 |
| DK152679C (en) | 1988-08-22 |
| ATA823178A (en) | 1981-02-15 |
| AU505434B1 (en) | 1979-11-22 |
| HU175521B (en) | 1980-08-28 |
| JPS54145283A (en) | 1979-11-13 |
| FI782871A (en) | 1979-05-19 |
| AT363887B (en) | 1981-09-10 |
| DK152679B (en) | 1988-04-11 |
| IL55969A0 (en) | 1979-01-31 |
| GB2008116A (en) | 1979-05-31 |
| GB2008116B (en) | 1982-03-17 |
| DK512978A (en) | 1979-05-19 |
| IT1101715B (en) | 1985-10-07 |
| DE2849393A1 (en) | 1979-05-23 |
| AR218348A1 (en) | 1980-05-30 |
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