PL114671B2 - Indicator for detection and semi-quantitative determination of glucose in physiological liquids - Google Patents
Indicator for detection and semi-quantitative determination of glucose in physiological liquids Download PDFInfo
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- PL114671B2 PL114671B2 PL20888078A PL20888078A PL114671B2 PL 114671 B2 PL114671 B2 PL 114671B2 PL 20888078 A PL20888078 A PL 20888078A PL 20888078 A PL20888078 A PL 20888078A PL 114671 B2 PL114671 B2 PL 114671B2
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- glucose
- indicator
- potassium
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims description 14
- 239000008103 glucose Substances 0.000 title claims description 14
- 238000001514 detection method Methods 0.000 title claims description 7
- 239000007788 liquid Substances 0.000 title description 2
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 12
- 108010015776 Glucose oxidase Proteins 0.000 claims description 11
- 239000004366 Glucose oxidase Substances 0.000 claims description 11
- 229940116332 glucose oxidase Drugs 0.000 claims description 11
- 235000019420 glucose oxidase Nutrition 0.000 claims description 11
- 239000003599 detergent Substances 0.000 claims description 9
- 102000003992 Peroxidases Human genes 0.000 claims description 8
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 8
- 239000011591 potassium Substances 0.000 claims description 8
- 229910052700 potassium Inorganic materials 0.000 claims description 8
- -1 amine molybdate Chemical class 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 6
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 5
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 5
- 229920000642 polymer Polymers 0.000 claims description 5
- 239000011734 sodium Substances 0.000 claims description 5
- 229910052708 sodium Inorganic materials 0.000 claims description 5
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 claims description 4
- 239000011609 ammonium molybdate Substances 0.000 claims description 4
- 235000018660 ammonium molybdate Nutrition 0.000 claims description 4
- 229940010552 ammonium molybdate Drugs 0.000 claims description 4
- 108010046301 glucose peroxidase Proteins 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims 1
- 210000002700 urine Anatomy 0.000 description 6
- 238000007046 ethoxylation reaction Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 239000001913 cellulose Substances 0.000 description 4
- 229920002678 cellulose Polymers 0.000 description 4
- 235000010980 cellulose Nutrition 0.000 description 4
- 229920006395 saturated elastomer Polymers 0.000 description 4
- 108010010803 Gelatin Proteins 0.000 description 3
- RWRDLPDLKQPQOW-UHFFFAOYSA-N Pyrrolidine Chemical compound C1CCNC1 RWRDLPDLKQPQOW-UHFFFAOYSA-N 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000008273 gelatin Substances 0.000 description 3
- 229920000159 gelatin Polymers 0.000 description 3
- 235000019322 gelatine Nutrition 0.000 description 3
- 235000011852 gelatine desserts Nutrition 0.000 description 3
- FTEGMIZQLGXBNA-UHFFFAOYSA-N 4-(4-amino-3-methylnaphthalen-1-yl)-2-methylnaphthalen-1-amine Chemical compound C1=CC=CC2=C(N)C(C)=CC(C=3C4=CC=CC=C4C(N)=C(C)C=3)=C21 FTEGMIZQLGXBNA-UHFFFAOYSA-N 0.000 description 2
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 229920002689 polyvinyl acetate Polymers 0.000 description 2
- 239000011118 polyvinyl acetate Substances 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 239000011684 sodium molybdate Substances 0.000 description 2
- 235000015393 sodium molybdate Nutrition 0.000 description 2
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 2
- XBSLKMQADAAKGP-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;phosphane Chemical compound P.OC(=O)CC(O)(C(O)=O)CC(O)=O XBSLKMQADAAKGP-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 239000001856 Ethyl cellulose Substances 0.000 description 1
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 1
- 241000206672 Gelidium Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000001785 acacia senegal l. willd gum Substances 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- UMGDCJDMYOKAJW-UHFFFAOYSA-N aminothiocarboxamide Natural products NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- 229920001429 chelating resin Polymers 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 230000000254 damaging effect Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 235000019325 ethyl cellulose Nutrition 0.000 description 1
- 229920001249 ethyl cellulose Polymers 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 235000010981 methylcellulose Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920001467 poly(styrenesulfonates) Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 235000019422 polyvinyl alcohol Nutrition 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
****1 ij «¦ Twórcywynalazki: Zbigniew Gregorowicz, Jerzy Ciba, Henryk Stec Uprawiony z patenti tymczasowego: Politechnika Slaska im. Wincentego Pstrowskiego.Gliwice (Polska) Wskaznik do wykrywania i pólilosciowego glukozy w plynach fizjologicznych 1 Przedmiotem wynalazku jest wskaznik do wykrywania i pólilosciowego oznaczania glukozy w plynach fizjolo¬ gicznych.Znany jest z brytyjskiego opisu patentowego nr 1018306 wskaznik do wykrywania i póilosciowego ozna¬ czania glukozy, który stanowi kompozycje ortotolidyny, jodku potasowego, molibdenianu sodu, potasu lub amonu oraz buforu i oksydazy glukozy. Inna znana z brytyjskiego opisu patentowego nr 949716 kompozycja zawiera ortotolidync. oksydaze glukozy, peroksydaze i bufor. Natomiast znana z literatury (Analyst 97, 142, 1972) kompozycja sklada sie z 4 aminoantypiryny, 2,4- dwuchloroienolu i poz^talych skladników znanych z patentu brytyjskiego nr 1018306. Wada omawianych wskazników jest brak zabezpieczenia przed niszczacym wplywem atmosfery, co powoduje skrócenie czasu zywotnosci wskaznika.Wskaznik wedlug wynalazku do wykrywania i póilos¬ ciowego oznaczania glukozy stanowi kompozycje skla¬ dajaca sie z 0,1-5% 3,3'-dwumetylonaftydyny i/lub fenylodwuaminy i/lub 4'-metoksy-4-aminodwufenylo- aminy, 3-50% buforu o pH= 4-9,4-15% oksydazy glu¬ kozy, 0,+2% peroksydazy lub 0,3-30%jodku potasowe¬ go i 0,1- 10% molibdenianu sodu, amonu lub potasu oraz korzystnie 0,01-0,5% detergentu niejonowego. Kompo¬ zycja osadzona jest w materiale hydrofilowym lub wymieniaczu jonowym anionoczynnym i zabezpieczona polimerem trudnoprzepuszczalnym dla skladników atmosfery i rozpuszczalnym w wodzie.Jako meterial hydrofilowy stosuje sie przykladowo: bibule, wlókna celulozowe,lniane, bawelniane, welniane, lignine, gabke naturalna, gabke poliuretanowa, zel krzemionkowy.Jako wymieniacz jonowy anionoczynny stosuje sie przykladowo: zywice monotiomocznikowa. Dowex N/CHjA, WofatytSBW.Jako polimer ochronny stosujesie przykladowo: winy- iopirolidyne, rozpuszczalne w wodzie pochodne celulozy takie jak: metyloceluloza, etyloceluloza, hydroksycelu- loza, rozpuszczalne w wodzie poliakryloamidy. alkohol poliwinylowy, zelatyne, agar-agar, gume arabska, polioctan celulozy, polioctan winylu. Kompozycja mozna oznaczyc 0,l-2g glukozy w 100cm3 plynu.Przyklad I. Bibule nasycono roztworem zawieraja¬ cym 0,1% 3.3'-dwumetylonaftydyny, 0,3%jodku potaso¬ wego, 0,3% molibdenianu sodu, 3% buforu fosforano¬ wego o pH = 4,7% oksydazy glukozy. 0.1% dekafenolu poliksyetylowanego o liczbie etoksylowania równej 4 jako detergenta niejonowego, 5% poliakryloamidu, 5% zelatyny.Za pomoca takiego wskaznika oznaczono 0,25% glukozy w moczu i plynach pozaustrojowych.Przyklad II. Pasek z plótna nasycono roztworem wodnym zawierajacym 5% 4-metoksy-4/-aminodwufeny- ominy, 10% molibdenianu amonu, 30% jodku potasu. 30% buforu cytrynianowego o pH= 6. 0,2% dedecylofe- nolu poUoksyetylowanego o liczbie etoksylowania = 10 jako detergenta i 5% oksydazy glukozy. Po wysuszeniupasek z naniesiona kompozycja pokryto warstwa ochronna, która przygotowano przez impregnacje cien¬ kiej folii celulozowej 5% roztworem polioctanu winylo¬ wego w acetonie.Za pomoca takiego wskaznika oznaczono We glukozy w moczu.Przyklad III. Pasek z gabki poliuretanowej nasy¬ cono roztworem uwierajacym 3% 3,?-dwumetylonaf- tydyny, 4% oksydazy glukozy, 0,4% peroksydazy, 15% buforu boraksowego o pH = 5, 0,01% undecyfenolu polioksyetylowanego o liczbie etoksylowania równej 14 jakp detergenta i 5% zelatyny. Po wysuszeniu kompozy¬ cje naniesiona na pasek pokryto warstwa polimeru ochronnego przez zanurzenie do roztworu 5 g polioctanu celulozy w 250cm3 acetonu.Za pomoca takiego wskaznika oznaczono 1,5% glukozy w moczu i plynach pozaustrojowych.Przyklad IV. Wymieniacz jonowy (amberlit La-1) Dsadzony w bibule nasycono roztworem zawierajacym 5% 3,J-dwumetylonaftydyny, 15% oksydazy glukozy, 1,0% peroksydazy, 5% alkoholu poliwinylowego, 15% buforu cytrynianowo-fosforowego, 0,1% fenolu polio- ksyetylowanego o liczbie etoksylowania równej 3 jako detergentu niejonowego. Po wysuszeniu bibule z nanie¬ siona kompozycja zabezpieczono siateczka z poliakrylu uszczelniona poliakrytoamidem.Za pomoca takiego wskaznika oznaczono 2% glukozy w moczu.PrzykladV. Przygotowanowskaznikjak wprzykla¬ dzieIV, zawierajacy w swoim skladzie 0,5%fenolupotio- ksyetylowanego o liczbie etoksylowania równej 3 jako detergenta niejonowego i 1% mieszaniny zawierajacej 50% 3,3'-dwumetylonaftydyny, 25% fenylodwuaminy i 25% 4-metoksy -4'-aminodwufenyloaminy. Wskaz¬ nikiem tym oznaczono 2% glukozy w moczu.Przyklad VI. Przygotowano wskaznik jak w przy¬ kladzie I, z ta róznica,ze nie zawieral on detergenta niejo¬ nowego. Wskaznikiem tym oznaczono 1,5% glukozy w moczu.Zastrzezenia patentowe 1. Wskaznik do wykrywania i pólilosciowego ozna¬ czania glukozy w plynach fizjologicznych zawierajacy oksydaze glukozy, peroksydaze lub molibdenian sodo¬ wy, potasowy badz aminowy i jodek potasowy, zaa- nieiay tyn, ze sklada sie z 0,1-5% 3J'-dwumetyionafty- dyny i/lub fenylodwuaminy i/lub 4'-metoksy-4-amino- dwufenyloaminy, 3-50% bufom o pH=4-9,4-15% oksydazy glukozy, 0,1-2,0% peroksydazy lub 0,3-30% odku potasowego, 0,1-10% molibdenianu sodu, potasu lub amonu,przy czym kompozycja skladnikówosadzona jest na materiale hydrofilowym lub wymieniaczu jono¬ wym anionoczynnym i zabezpieczona polimerem trud- noprzepuszczalnym dla skladników atmosfery i rozpusz¬ czalnym w wodzie. 2. Wskaznik wedlug zastrz, 1, zaaakray tym, ze zawiera 0,01-0,5% detergentu niejonowego.Prac Poligral. UP PRL. Naklad 120egz.Cena UW zl PL**** 1 ij «¦ Creators of the invention: Zbigniew Gregorowicz, Jerzy Ciba, Henryk Stec Entitled by a temporary patent: Politechnika Slaska im. Wincentego Pstrowskiego.Gliwice (Poland) Indicator for the detection and semiquantitative glucose in physiological fluids 1 The subject of the invention is an indicator for the detection and semiquantitative determination of glucose in physiological fluids. It is known from the British patent specification No. 1018306 an indicator for the detection and semiquantitative determination of glucose which is a composition of orthotholidine, potassium iodide, sodium, potassium or ammonium molybdate and a buffer and glucose oxidase. Another composition known from British Patent Specification No. 949716 comprises orthotholideinc. glucose oxidase, peroxidase and buffer. On the other hand, the composition known from the literature (Analyst 97, 142, 1972) consists of 4 aminoantipyrine, 2,4-dichloroienol and other ingredients known from British patent No. 1018306. The disadvantage of these indicators is the lack of protection against the damaging effects of the atmosphere, which shortens The indicator according to the invention for the detection and semi-quantitative determination of glucose is a composition consisting of 0.1-5% of 3,3'-dimethylnaphthidine and / or phenyldiamine and / or 4'-methoxy-4-aminodiphenyl- amine, 3-50% buffer with pH = 4-9.4-15% glucose oxidase, 0.2% peroxidase or 0.3-30% potassium iodide and 0.1-10% sodium molybdate. ammonium or potassium and preferably 0.01-0.5% of a non-ionic detergent. The composition is embedded in a hydrophilic material or an anionic ion exchanger and protected with a polymer that is hardly permeable to atmospheric components and soluble in water. Silica gel. As an anionic ion exchanger, for example, monothiourea resins are used. Dowex N / CHjA, WofatytSBW. As a protective polymer, for example, the following are used: vin- and pyrrolidine, water-soluble cellulose derivatives such as: methyl cellulose, ethyl cellulose, hydroxycellulose, water-soluble polyacrylamides. polyvinyl alcohol, gelatin, agar-agar, arabic gum, cellulose polyacetate, polyvinyl acetate. The composition can be determined with 0.1-2 g of glucose in 100 cm3 of liquid. Example I. Blotting paper is saturated with a solution containing 0.1% 3.3'-dimethylnaphthidine, 0.3% potassium iodide, 0.3% sodium molybdate, 3% buffer phosphate at pH = 4.7% glucose oxidase. 0.1% polyoxyethylated decaphenol with an ethoxylation number of 4 as a non-ionic detergent, 5% polyacrylamide, 5% gelatin. This indicator was used to determine 0.25% glucose in urine and extracorporeal fluids. Example II. The canvas strip was saturated with an aqueous solution containing 5% 4-methoxy-4 / -aminodiphenylamine, 10% ammonium molybdate, 30% potassium iodide. 30% citrate buffer at pH = 6. 0.2% polyoxyethylated dedecylphenol with ethoxylation number = 10 as detergent and 5% glucose oxidase. After drying, the strip with the applied composition was covered with a protective layer, which was prepared by impregnating a thin cellulose film with a 5% solution of polyvinyl acetate in acetone. The glucose in urine was determined using this indicator. Example III. The strip of polyurethane sponge was saturated with 3% 3, α-dimethylnaphthidine holding solution, 4% glucose oxidase, 0.4% peroxidase, 15% borax buffer pH = 5, 0.01% polyoxyethylated undecyphenol with an ethoxylation number of 14 like detergent and 5% gelatin. After drying, the compositions were covered with a protective polymer layer by dipping into a solution of 5 g of cellulose polyacetate in 250 ml of acetone. 1.5% of glucose in urine and extracorporeal fluids was determined using this indicator. Example IV. Ion exchanger (Amberlite La-1) Filled in a filter paper, saturated with a solution containing 5% 3, J-dimethylnaphthidine, 15% glucose oxidase, 1.0% peroxidase, 5% polyvinyl alcohol, 15% citrate-phosphorus buffer, 0.1% phenol polyoxyethylated with an ethoxylation number of 3 as a non-ionic detergent. After drying, the blotting paper with the applied composition was secured with a polyacrylic mesh sealed with polyacrythamide. 2% of the glucose in urine was determined by this indicator. The indicator was prepared as in Example IV, containing 0.5% of phenol-ethoxylated with an ethoxylation number of 3 as a non-ionic detergent and 1% of a mixture of 50% 3,3'-dimethylnaphthidine, 25% phenyldiamine and 25% 4-methoxy-4 '. -aminodiphenylamine. This index determines 2% of glucose in urine. Example VI. An indicator was prepared as in Example 1, except that it did not contain a non-ionic detergent. This indicator was determined for 1.5% of glucose in urine. Patent claims 1. Indicator for the detection and semiquantitative determination of glucose in physiological fluids containing glucose oxidase, peroxidase or sodium, potassium or amine molybdate and potassium iodide, it consists of 0.1-5% of 3J'-dimethionaphthidine and / or phenyldiamine and / or 4'-methoxy-4-amino-diphenylamine, 3-50% in buffers with pH = 4-9.4-15% glucose oxidase, 0.1-2.0% peroxidase or 0.3-30% potassium odor, 0.1-10% sodium, potassium or ammonium molybdate, the ingredient composition being deposited on a hydrophilic material or an anionic ion exchanger and protected with a polymer that is difficult to penetrate to atmospheric components and soluble in water. 2. The indicator according to claim 1, additionally containing 0.01-0.5% of non-ionic detergent. Work Poligral. UP PRL. Mintage 120 copies. Price UW zl PL
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL20888078A PL114671B2 (en) | 1978-08-03 | 1978-08-03 | Indicator for detection and semi-quantitative determination of glucose in physiological liquids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL20888078A PL114671B2 (en) | 1978-08-03 | 1978-08-03 | Indicator for detection and semi-quantitative determination of glucose in physiological liquids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL208880A1 PL208880A1 (en) | 1979-07-02 |
| PL114671B2 true PL114671B2 (en) | 1981-02-28 |
Family
ID=19990940
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL20888078A PL114671B2 (en) | 1978-08-03 | 1978-08-03 | Indicator for detection and semi-quantitative determination of glucose in physiological liquids |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL114671B2 (en) |
-
1978
- 1978-08-03 PL PL20888078A patent/PL114671B2/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL208880A1 (en) | 1979-07-02 |
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