PH27110A - New indolypiperidine compounds processes for the preparation thereof and pharmaceutical composition comprising the same - Google Patents

Description

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. NEW INDOLYLPIPERIDINE COMPOUNDS, PROCESSES co FOR ThE PREPARATION THEREOF AND PHARMA-

CEUTICAL COMPOSITION COMPRISING THE SAME

This invention relates to new indolylpiperidine compounds and prarmaceutically acceptable salts thereof.

More particularly, it relates to new indolylpiperidine - compounds and pharmaceutically acceptable salts thereof oO 5 which have antiallergic activity, to processes for the preparation thereof, to a pharmaceutical composition com- : prising the same and to a method for the treatment of : allergic disease in human being or animals. . One object of this invention is to provide new indolylpiperidine compounds and pharmaceutically acceptable salts thereof which possess antiallergic activity. : Another object of this invention is to provide processes for the preparation of said indolylpiperidine compounds or salts thereof.

A further object of this invention is to provide a

'- ? - pharmaceutical composition comprising, as an active ingredient, said indolylpiperidine compounds or pharmaceutically acceptable salts thereof. ; Still further object of this invention is to provide a therapeutical method for the treatment of allergic disease such as allergic asthma, allergic

J rhinitis, allergic conjunctivitis, chronic urticaria, or the like, in himan being or animals.

Some indolylpiperidine compounds having anti- i 10 allergic activity have been known as described in

British Patent Application Publication No. 2093455. : Some amide derivatives having anti-allergic i

Eo activity have been known as described in European

Patent Application Publication No. 157420. p : 15 The object indolylpiperidine compounds of this

P

; invention are new and can be represented by the following general formula [I] : ; - ( 'N-A-NHCO-B-R' i nN” [1]

H

IF . } ) oD i © 25 wherein rt is aryl substituted with substituent(s) bo ! selected from the group consisting oo of hydroxy, protected hydroxy, ; halogen and lower alkoxy, i A is lower alkylene, and 1 : 30 B is lower alkenylene. :

The object compound [I] or its salt can be prepared by processes as illustrated in the following reaction schemes. i {

- 3 =

Process 1 rR! -B-coon [III] or its reactive derivative at the carboxy group or 1

N-A-NH, a salt thereof Z N-A-NHCO-B-R —_— CO

J

N N

H H

[II] [I] or its reactive derivative or its salt at the amino group or a

O salt thereof

Process 2

Elimination of the hydroxy- protective group

N-A-NHCO-B-RY —— — 1 a = | N-A-NHCO-B-R_

N XN N

H H

[Ta] [Ib] or its salt or its salt (1) 25 :

Process 3

Acylation 1 JA f-A-NHCO-B-R —— Ye R.

I ) NNN

H H

[Ib] [Ic] or its salt or its salt

Za - wherein R is aryl substituted with protected hydroxy, with protected hydroxy and halogen, or with protected hydroxy and lower alkoxy,

R, is aryl substituted with hydroxy, with hydroxy and halogen, or with hydroxy and lower alkoxy, rR is aryl substituted with acyloxy, with acyloxy and halogen, or with acyloxy and lower alkoxy, and

RY, A and B are each as defined above. ‘10

In the above and subsequent descriptions of the

O present specification, suitable examples of the various definitions to be included within the scope of the invention are explained in detail in the following.

The term "lower" is intended to mean a group having 1 to 6 carbon atom(g), unless otherwise provided.

Suitable "aryl" may be phenyl, naphthyl, phenyl substituted with lower «tkyl [e.g. tolyl, mesityl, cumenyl, xylyl, diethylphenyl, di’sopropylphenyl, di-tert-butyl- phenyl, etc.] or the like. i

Suitable "protected hydroxy" may be substituted lower alkoxy such as lower alkoxy (lower)alkoxy (lower)- alkoxy [e.g. methoxyethoxymethoxy, etc.], substituted . or unsubstituted ar (lower)alkoxy [e.g. benzyloxy, .

O 25 nitrobenzyloxy, etc.], acyloxy such as lower alkanoyloxy

Co [e.g. formyloxy, acetyloxy, propionyloxy, butyryloxy, : isobutyryloxy, valeryloxy, isovaleryloxy, pivaloyloxy, . hexanoyloxy, 3,3-dimethylbutyryloxy, etc.], lower alkoxy- carbonyloxy [e.g. methoxycarbonyloxy, ethoxycarbonyloxy, propoxycarbonyloxy, isopropoxycarbonyloxy, butoxycarbonyl- oxy, isobutoxycarbonyloxy, tert-butoxycarbonyloxy, pentyloxycarbonyloxy, hexyloxycarbonyloxy, etc.], sulfonyloxy (e.g. mesyloxy, tosyloxy, benzenesulfonyloxy, etc.], substituted or unsubstituted ar (lower)alkoxy- carbonyloxy (e.g. benzyloxycarbonyloxy, bromobenzyloxy- carbonyloxy, etc.] etc.,

- 5 = tri(lower)alkylsilyloxy [e.g. trimethylsilyloxy, etc.] or the like.

Suitable "halogen" is fluorine, chlorine, bromine and iodine.

Suitable "acyloxy" may be the same as above-mentioned acyloxy enumerated for protected hydroxy.

Suitable "lower alkoxy" may be a straight or branched one such as methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, tert-butoxy, pentyloxy, hexyloxy or the like, in which the preferable one is

C17Cy alkoxy and the most preferable one is methoxy.

Od Preferable examples of "aryl substituted with sub- stituent(s) selected from the group consisting of hydroxy, protected hydroxy, halogen and lower alkoxy" may be mono-, or di-, or trihydroxyphenyl; mono-, or di-, or tri(halo)phenyl (e.g. chlorophenyl, fluorophenyl, dichlorophenyl, trifluorophenyl, etc.]; mono-, or di-, or tri(lower)alkylphenyl [e.g. tolyl, mesityl, cumenyl, xylyl, ethylphenyl, diethylphenyl, isopropylphenyl, diisopropylphenyl, di-tert-butylphenyl, etc.]; : mono-, or di-, or tri(lower)alkoxyphenyl [e.g. methoxy- phenyl, ethoxyphenyl, dimethoxyphenyl, trimethoxyphenyl, diethoxyphenyl, diisopropoxyphenyl, etc.]; mono-, or dihydroxy and mono-, or di (lower)alkoxy substituted

O 25 phenyl [e.q. me thoxy (hydroxy) phenyl, ethoxy (hydroxy) - phenyl, isopropoxy (hydroxy)phenyl, dimethoxy (hydroxy)- phenyl, diethoxy (hydroxy)phenyl, diisopropoxy (hydroxy)- phenyl, methoxy (dihydroxy)phenyl, methoxy (ethoxy) - hydroxyphenyl, etc.]; mono-, or dihydroxy and mono-, or di (lower)alkyl substituted phenyl [e.g. methyl (hydroxy)- phenyl, ethyl (hydroxy)phenyl, propyl (hydroxy) pnenyl, . isopropyl (hydroxy) phenyl, dimethyl (hydroxy) phenyl, diethyl (hydroxy) phenyl, diisopropyl (hydroxy)phenyl, di-tert-butyl (hydroxy) phenyl, methyl (dihydroxy) phenyl, methyl (ethyl) hydroxyphenyl, etc.]; mono-, or dihydroxy and mono-, or dihalo substituted phenyl [e.g. chloro- (hydroxy) phenyl, dichloro (hydroxy)phenyl, fluoro- (hydroxy) phenyl, chloro (dihydroxy)phenyl, etc.]; mono-, or di-, or tri-protected hydroxy substituted phenyl such as mono-, or di-, or tri{lower alkoxy (lower)- alkoxy (lower) alkoxylphenyl [e.g. mono-, or di-, or : tri (methoxyethoxymethoxy) phenyl, etc.], mono-, or di-, or triacyloxyphenyl [e.g. mono-, or di-, or tri(lower)- alkanoyloxyphenyl (e.g. formyloxyphenyl, acetyloxyphenyl, propionyloxyphenyl, diacetyloxyphenyl, dipropionyloxy- phenyl, triacetyloxyphenyl, etc.), mono-, or di-, or

O) tri (lower)alkoxycarbonyloxyphenyl (e.g. methoxycarbonyl- oxyphenyl, ethoxycarbony.oxyphenyl, diethoxycarbonyloxy- phenyl, triethoxycarbonyloxyphenyl, etc.), etc.] or the like; mono-, or di (lower)alkoxy and mono-, or di- protected hydroxy substituted phenyl such as mono-, or di (lower)alkoxy and mono-, or di[lower alkoxy (lower)- alkoxy (lower)alkoxy]substituted phenyl [e.g. methoxy- (methoxyethoxymethoxy) phenyl, ethoxy (methoxyethoxy- methoxy) phenyl, dimethoxy (methoxyethoxymethoxy)phenyl, diethoxy (methoxyethoxymethoxy)phenyl, diisopropoxy- (methoxyethoxymethoxy) phenyl, etc.], mono-, or diacyloxy and mono-, or di (lower)alkoxy substituted phenyl [e.g. mono-, or di(lower)alkanoyloxy and mono-,

GO 25 or di (lower)alkoxy substituted phenyl (e.g. acetyloxy- (methoxy) phenyl, propionyloxy (methoxy)phenyl, acetyloxy- (ethoxy) phenyl, acetyloxy (dimethoxy) phenyl, propionyloxy- (dimethoxy) phenyl, acetyloxy (diethoxy)phenyl, acetyloxy- (diisopropoxy) phenyl, Jdiacetyloxy (methoxy)phenyl, etc.), mono-, or di (lower)alkoxycarbonyloxy and mono-, or di (lower)alkoxy substituted phenyl (e.g. methoxycarbonyl- oxy (methoxy) phenyl, ethoxycarbonyloxy (methoxy) phenyl, ethoxycarbonyloxy (ethoxy) phenyl, methoxycarbonyloxy- (dimethoxy) phenyl, ethoxycarbonyloxy (dimethoxy)phenyl, ethoxycarbonyloxy (diethoxy) phenyl, ethoxycarbonyloxy- (diisopropoxy) phenyl, etc.), etc.] or the like;

mono-, or di(lower)alkyl and mono-, or di- protected hydroxy substituted phenyl such as mono-, or di (lower)alkyl and mono-, Or di [lower alkoxy (lower)- alkoxy (lower) alkoxy] substituted phenyl [e.g. methyl- (methoxyethoxymethoxy) phenyl, ethyl (methoxyethoxymethoxy)- phenyl, dimethyl (methoxyethoxymethoxy) phenyl, diethyl- (methoxyethoxymethoxy) phenyl, diisopropyl (methoxyethoxy- me thoxy) phenyl, di-tert-butyl (methoxyethoxymethoxy)phenyl, etc.], mono-, or diacyloxy and mono-, Or di (lower)alkyl substituted phenyl [e.g. mono-, or di (lower) alkanoyloxy and mono-, or di(lower)alkyl substituted phenyl (e.g.

O acetyloxy (methyl) phenyl, propionyloxy (methyl)phenyl, acetyloxy (ethyl) phenyl, acetyloxy (dimethyl) phenyl, propionyloxy (dimethyl) phenyl, acetyloxy (diethyl) phenyl, acetyloxy (diisopropyl) phenyl, diacetyloxy (methyl) phenyl, etc.), mono-, or di(lower)alkoxycarbonyloxy and mono-, or di (lower)alkyl substituted phenyl (e.g. methoxycarbonyl- : oxy (methyl) phenyl, ethoxycarbonvluuy (methyl) phenyl, : ethoxycarbonyloxy (ethyl) phenyl, me thoxycarbonyloxy- (dimethyl)phenyl, ethoxycarbonyloxy (dimethyl)phenyl, ethoxycarbonyloxy (diethyl) phenyl, ethoxycarbonyloxy- (diisopropyl)phenyl, etc.), etc.] or the like; and mono-, or dihalo and mono-, or di- protected hydroxy substituted phenyl such as mono-, or dihalo and mono-, 0 25 or di[lower alkoxy (lower) alkoxy (lower)alkoxyl- . substituted phenyl [e.g. chloro (methoxyethoxymethoxy)- .phenvyl, dichloro (methoxyethoxymethoxy) phenyl, fluoro- (methoxyethoxymethoxy) phenyl, etc.], mono-, or diacyloxy and mono-, or dihalo substituted phenyl [e.g. mono-, Or di (lower)alkanoyloxy and mono-, or dihalo substituted phenyl (e.g. acetyloxy (chloro)phenyl, propionyloxy- (chloro)phenyl, acetyloxy (dichloro)phenyl, etc.), mono-, or di (lower)alkoxycarbonyloxy and mono-, Or dihalo substituted phenyl (e.g. methoxycarbonyloxy (chloro) phenyl, ethoxycarbonyloxy (chloro) phenyl, ethoxycarbonyloxy- (dichloro)phenyl, etc.), etc.], or the like.

Preferable examples of "aryl substituted with protected hydroxy, with protected hydroxy and halogen, or with protected hydroxy and lower alkoxy" may be the same as above-mentioned mono-, or di-, or tri- protected hydroxy substituted phenyl; mono-, or dihalo and mono-, or di- protected hydroxy substituted phenyl; mono-, or di(lower)alkoxy and mono-, or di- protected hydroxy substituted phenyl; and mono-, or di(lower)alkyl and mono-, or di- protected hydroxy substituted phenyl.

O Preferable examples of "aryl substituted with hydroxy, with hydroxy and halogen, or with hydroxy and lower alkoxy" may be the same as above-mentioned mono-, or di-, or trihydroxy phenyl; monc-, or dihydroxy and mono-, or dihalo substituted phenyl; mono-, or dihydroxy and mono-, or di (lower)alkoxy substituted phenyl; and mono-, or dihydroxy and mono-, or di (lower)alkyl : substituted phenyl.

Preferable examples of "aryl substituted with acyloxy, with acyloxy and halogen, or with acyloxy and : lower alkoxy" may be the same as above-mentioned ‘ ® 25 mono-, or di-, or triacyloxyphenyl; mono-, or diacyloxy and mono-, or dihalo substituted phenyl; : mono-, or diacyloxy and mono-, or di (lower)alkoxy . substituted phenyl; and mono- or diacyloxy and mono- or di (lower)alkyl substituted phenyl.

Suitable "lower alkylene" nay be a straight or branched one such as methylene, ethylene, trimethylene, : tetramethylene, ethylethylene, propylene, pentamethylene, hexamethylene or the like.

Suitable "lower alkenylene" may be vinylene,

propenylene, butenylene, pentenylene, butadienylene, pentadienylene or the like.

Suitable pharmaceutically acceptable salts of the object compound [I] are conventional non-toxic salts and include a metal salt such as an alkali metal salt [e.g. sodium salt, potassium salt. etc.] and an alkaline earth metal salt [e.g. calcium salt, magnesium salt, etc.], an acid addition salt such as an organic acid addition salt [e.g. formate, acetate, trifluoroacetate, fumarate, maleate, tartrate, methanesulfonate, benzenesulfonate, toluenesulfonate, etc.], an inorganic

O acid addition salt [e.g. hydrochloride, hydrobromide, sulfate, phosphate, etc.], a salt with an amino acid [e.g. aspartic acid salt, glutamic acid salt, etc.] and the like.

With respect to the salts of the compounds [Ia], [Ib] and [Ic] in the Processes 2 and 3,1it is to be noted that these compounds are included within the scope of the compound [I], :

Q i ~ 25 ’ to be continued on the next page and accordingly the suitable examples of the salts of these compounds are to be referred to those as exemplified for the object compound [I]. .

The processes for preparing the object compounds [I] of the present invention are explained in detail in the following.

Process 1

The object compound [I] or its salt can be pre- pared by reacting a compound [II] or its reactive

O derivative at the amino group or a salt thereof with a compound [III] or its reactive derivative at the carbexy group or a salt thereof.

Suitable reactive derivative at the z2mino group of the compound [II] may include Schiff's base type imino or its tautomeric enamine type isomer forme” by the reaction of the compound [II] with a carbony! compound such as aldehyde, ketone or the like; a silyl derivative formed by the reaction of the compound [II] with a silyl compound such as bis (trimethylsilyl)acetamide, mono (trimethylsilyl)acetamide, bis (trimethylsilyl)urea or the like; a derivative formed by reaction ef the

Q 25 compound [II] with phosphorus trichloride or phosgene, and the like.

Suitable salts of the compound [II] and its reactive derivative can be referred to the acid addition salt as exemplified for the compound [I].

Suitable reactive derivative at the carboxy group of tne compound [III] may include an acid halide, an acid anhydride, an activated amide, an activated ester, and the like. Suitable examples of the reactive derivatives may be an acid chloride; an acid azide; a mixed acid anhydride with an acid such as substituted phosphoric

CL i acid [e.g. dialkylphosphoric acid, phenylphosphoric acid, diphenylphosphoric acid, dibenzylphosphoric acid, halogenated phosphoric acid, etc.], dialkylphosphorous acid, sulfurous acid, thiosulfuric acid, sulfuric acid, sulfonic acid [e.g. methanesulfonic acid, etc.], aliphatic carboxylic acid [e.g. acetic acid, propionic i acid, butyric acid, isobutyric acid, pivalic acid, i pentanoic acid, isopentanoic acid, 2-ethylbutyric acid, : | trichloroacetic acid, etc.] or aromatic carboxylic acid ; 10 " [e.g. benzoic acid, etc.]; a symmetrical acid anhydride; an activated amide with imidazole, 4-substituted imidazole, ® dimethylpyrazole, triazole or tetrazole; or an activated ; ester [e.g. cyanomethyl ester, methoxymethyl ester, dimethyliminomethyl [ (Hy) ,Ni=CH-] ester, vinyl ester, propargyl ester, p-nitrophenyl ester, 2,4-dinitrophenyl ester, trichlorophenyl ester, pentachlorophenyl ester, mesylphenyl ester, phenylazophenyl ester, phenyl thioester, p-nitrophenyl thioester, p-cresyl thioester, carboxymethyl thinester, pyranyl ester, pyridyl ester, piperidyl ester, 8-quinolyl thicester, etc.], or .an ester with a N-hydroxy compound (e.g. N,N-dimethyl- : hydroxylamine, l-hydroxy-2-(1H)-pyridone, N-hydroxy- succinimide, N-hydroxyphthalimide, l-hydroxy-1H- benzotriazole, etc.], and the like. These reactive deri-.

OQ as vatives can optionally be selected from them according ! to the kind of the compound [III] to be used. : Suitable salts Of the compound [III] and its reactive derivative may be a base salt such as an alkali metal salt [e.g. sodium salt, potassium salt, etc.], an alkaline earth metal salt [e.g. calcium salt, magnesium salt, etc.], an ammonium salt, an organic base salt [e.g. trimethylamine salt, triethylamine salt, pyridine salt, picoline salt, dicyclohexylamine salt, N,N'-dibenzylethylenediamine salt, etc.], or the like.

The reaction is usually carried out in a conven- tional solvent such as water, alcohol [e.qg. methanol, ethanol, etc.], acetone, dioxane, acetonitrile, chloroform, methylene chloride, ethylene chloride, tetrahydrofuran, ethyl acetate, N,N-dimethylformamide, pyridine or any other organic solvent which does not i adversely influence the reaction. These conventional ! solvent may also be used in a mixture with water.

In this reaction, when the compound [III] is used in a free acid form or its salt form, the reaction is preferably carried out in the presence of a conventional () condensing agent such as N,N'-dicyclohexylcarbodiimide; ; N-cyclohexyl-N'-morpholinoethylcarbodiimide;

N-cyclohexyl-N'-(4-diethylaminocyclohexyl)carbodiimide;

N,N'-diethylcarbodiimide, N,N'-diisopropylcarbodiimide;

N-ethyl-N'-(3-dimethylaminopropyl)cagbodiimide; N,N'- : carbonylbis- (2-methylimidazole); pentamethyleneketene-

N-cyclohexylimine; diphenylketene-N-cyclohexylimine; ethoxyacetylene; l-alkoxy-l-chloroethylene; trialkyl phosphite; ethyl polyphosphate; isopropyl polyphosphate; phosphorus oxychloride (phosphoryl chloride); phosphorus trichloride; diphenyl phosphorylazide; diphenylphosphinic chloride; thionyl chloride; oxalyl chloride; lower alkyl haloformate (e.g. ethyl chloroformate, ) 25 isopropyl chloroformate, etc.]; triphenylphosphine;

Co 2-ethyl-7-hydroxybenzisoxazolium salt; 2-ethyl-5- (m- sul fophenyl) isoxazolium hydroxide intramolecular salt; ° 1-(p-chlorobenzenesulfonyloxy)-6-chloro-1lHE-benzotriazole; so-called Vilsmeier reagent prepared by the reaction of

N,N-dimethylformamide with thionyl chloride, phosgene, trichloromethvl chloroformate, phosphorus oxychloride, etc.; or the like. : The reaction may also be carried out in the presence of an inorganic or organic base such as an alkali metal bicarbonate, tri(lower)alkylamine, pyridine, i

- 13 ~-

N- (lower) alkylmorpholine, N,N-di(lower)alkylbenzylamine, or the like.

The reaction temperature is not critical, and the reaction is usually carried out under cooling to warming.

Process 2

The compound [Ib] or its salt can be prepared by subjecting a compound [Ia] or its salt to elimination reaction of the hydroxy-protective group.

This reaction is carried out in accordance with a conventional method such as hydrolysis, reduction or

O) the like.

The hydrolysis is preferably carried out in the presence of a base or an acid including Lewis acid.

Suitable base may include an inorganic base and an organic base such as an alkali metal [e.g. sodiuin, potassium, etc.], an alkaline earth metal [e.g. magnesium, calcium, etc.], the hydroxide or carbonat: or bicarbonate thereof, trialkylamine [e.g. trimethylamine, triethylamine, etc.l, picoline 1,5-diazabicyclo[4.3.0]non-5-ene, 1,4-diazabicyclo- [2.2.2]octane, 1,8-diazabicyclo[5.4.0]undec-7-ene, or the like. ~ Suitable acid may include an organic acid -[e.qg.

Q 25 formic acid, acetic acid, propionic acid, trichloro- acetic acid, trifluoroacetic acid, methanesulfonic acid, p-toluenesulfonic acid, etc.] and an inorganic acid [e.gq. hydrochloric acid, hydrobromic acid, sulfuric acid, hydrogen chloride, hydrogen bromide, hydrogen fluoride, etc.].

The elimination using Lewis acid such as trihalo- acetic acid (e.g. trichloroacetic acid, trifluoroacetic acid, etc.] or the like is preferably carried out in the presence of cation trapping agents [e.g. anisole, phenol, etc.].

The reaction is usually carried out in a solvent such as water, an alcohol [e.g. methanol, ethanol, etc.], methylene chloride, chloroform, tetrachloromethane, tetrahydrofuran, a mixture thereof or any other solvent which does not adversely influence the reaction.

A liquid base or acid can be also used as the solvent.

The reaction temperature is not critical and the reaction is usually carried out under cooling to heating.

The reduction method applicable for the elimination reaction may include chemical reduction and catalytic reduction.

Suitable reducing agents to be used in chemical

O reduction are a combination of metal [e.g. tin, zinc, - iron, etc.] or metallic compound (e.g. chromium chloride, chromium acetate, etc.] and an organic or inorganic acid e.g. formic acid, acetic acid, propionic acid, trifluoroacetic acid, p-toluenesulfonic acid, hydrochloric acid, hydrobromic acid, etc.].

Suitable catalysts tec be used in catalytic reduction are conventional ones such as platinum catalysts (e.g. platinum plate, spongy platinum, platinum black, colloidal platinum, platinum oxide, platinum wire, etc.], palladium catalysts e.g. =pongy palladium, palladium black, palladium oxide, palladium on carbon, colloidal palladium, palladium on barium .sulfate, palladium on

QO 25 barium carbonate, etc.], nickel catalysts '[e.g. reduced nickel, nickel oxide, Raney nickel, etc.], cobalt catalysts [e.g. reduced cobalt, Raney cobalt, etc.}, iron catalysts [e.g. reduced iron, Raney iron, etc.]}, copper catalysts e.g. reduced copper, Raney copper,

Ullman copper, etc.} and the like.

The reduction is usually carried out in a conven- tional solvent which does not adversely influence the reaction such as water, methanol, ethanol, propanol,

N,N-dimethylformamide, or a mixture thereof.

Additionally, in case that the above-mentioned acids to be used in chemical reduction are in liquid, they can also be used as a solvent. Further, a suitable solvent to be used in catalytic reduction may be the above- mentioned solvent, and other conventional solvent such as diethyl ether, dioxane, tetrahydrofuran, etc., or a mixture thereof. i The reaction temperature of this reduction is not critical and the reaction is usually carried out under cooling to heating.

Co 10

Co Process 3 @ The object compound [Ic] or its salt can be prepared by reacting a compound [Ib] or its salt with an acylating agent.

Suitable acylating agents are the corresponding carboxylic acid or sulfonic acid compounds, which are represented by the formula : R®-OH wherein rR? is acyl, and reactive derivatives thereof.

Suitable "acyl" may be the same as acyl group for "acyloxy" as exemplified above.

Suitable said reactive derivatives can be referred to the ones at the carboxy groups of the compound [III] as exemplified above. The kind of such reactive . derivatives can be selected depending on the .kind of

Q 25 acyl group to be introduced. oo The reaction is usually carried out in a conven- : tional solvent, such as methylene chloride, chloroform, ) benzene, toluene, pyridine, diethyl ether, dioxane, tetrahydrofuran, acetone, acetonitrile, ethyl acetate,

N,N-dimethylformamide or any other organic solvent which does not adversely affcct the reaction. In case that the acylating agent is liquid, it can also be used as a solvent.

In case that the carboxylic acid compounds are used as acylating agent in the free acid form or salt form, it is preferable to carry out the reaction in the presence bo - 16 - : of a conventional condensing agent such as N,N'- dicyclohexylcarbodiimide or the like. i The reaction temperature is not critical and the reaction can be carried out under cooling, at ambient temperature, or under heating. : This reaction is preferably carried out in the presence of an inorganic base, for example an alkali metal hydroxide such as sodium hydroxide or potassium hydroxide, or an alkali metal carbonate or hydrogen carbonate such as sodium carbonate, potassium carbonate, : sodium hydrogen carbonate or potassium hydrogen carbonate,

O or in the presence of an organic base, for example a tertiary amine such as triethylamine, pyridine,

N-methylmorpholine or N,N-dimethylaniline.

Among the starting compounds [II] and [III], some of them are new and can be prepared by processes as : ! illustrated in the follpwing reaction schemes. ~ Process A - R3-A-X (V] or its salt ; O NH N-A-R> | I 3 | C I ]

N Step 1 N

H H ta [VI] or its salt or its salt i 30 ! Elimination of the amino-protective group 7] { N-A-NH, — v

H

Step 2 [IT] or its salt !

Process B 0 ©2Ms0 boc -s'-r? (vir:

CHO 2

Ce . 1 (Wittig reaction) 1 4

R™-CHO _ >» R"-CH=CH-B'-R [VII] Step 1 [IX] or its salt or its salt

Elimination of the carboxy-protective

QO) group L tne R™~CH=CH-B'-COOH

Step 2 [IIIa] or its salt wherein R> is protected amino, r} is protected carboxy,

B' is lower alkylene or lower alkenylene,

X is a leaving group, rt and A are each as defined above. ’ Suitable "protected amino" may be acylamino such as

O 25 substituted or unsubstituted lower alkanoylamino [e.g. formylamino, acetylamino, propionylamino, trifluorocacetyl- : amino, etc.], phthaloylimino, lower alkoxycarbonylamino ’ [e.g. tert-butoxycarbonylamino, tert-amyloxycarbonylamino, etc.], substituted or unsubstituted aralkyloxycarbonylamino [e.g. benzyloxycarbonylamino, p-nitrobenzyloxycarbonylamino, etc.], substituted or unsubstituted arenesulfonylamino [e.g. benzenesul fonylamino, tosylamino, etc.], nitrophenylsul fenylamino, or the like, aralkylamino [e.g. tritylamino, benzylamino, etc.] or the like.

Suitable "protected carboxy" may be carboxy group protected by conventional protective group such as : lower alkoxycarbonyl [e.g. methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, isopropoxycarbonyl, botoxycarbonyl, sec-butoxycarbonyl, isobutoxycarbonyl, tert-butoxycarbonyl, : 5 pentyloxycarbonyl, neopentyloxycarbonyl, hexyloxycarbonyl, etc.], optionally substituted ar (lower)alkoxycarbonyl for example, mono or di or triphenyl (lower)alkoxycarbonyl which may be substituted with nitro [e.g. benzyloxy- carbonyl, 4-nitrobenzyloxycarbonyl, benzhydryloxy- carbonyl, trityloxycarbonyl, etc], or the like.

Suitable "leaving group" may be an acid residue

O such as halogen [e.g. chlorine, bromine, fluorine and iodine], sulfonyloxy [e.g. mesyloxy, tosyloxy, phenylsulfonyloxy, etc.] or the like.

The processes for preparing the starting compounds are explained in detail in the following. ’ Process A

Step 1

The compound [VI] or its salt can be prepared by reacting a compound [IV] or its salt with a compound {V] or its salt.

Suitable salts of the compounds (IV], [V] and [VI]

O can be referred to the acid addition salts as exemplified for the compound {I}.

This reaction is usually carried out in a conventional solvent such as water, an alcohol [e.g. methanol, ethanol, isopropyl alcohol, etc.], dioxane, tetrahydrofuran, N,N-dimethylformamide, methylene chloride, chloroform, tetrachloromethane, or any other conventional solvent which does uot adversely affect this reaction, or a mixture thereof.

The reaction is carried out at ambient temperature, under warming or under heating, although the reaction temperature is not critical.

This reaction can also be conducted in the presence of an inorganic base, for example an alkali metal hydroxide such as sodium hydroxide or potassium hydroxide, or an alkali metal carbonate or hydrogen carbonate such as sodium carbonate, potassium carbonate, sodium hydrogen carbonate or potassium hydrogen carbonate, or in the presence of an organic base, for example a tertiary amine such as triethylamine, pyridine or N,N-dimethylaniline.

This reaction can also be performed in the presence of an alkali metal halide such as sodium iodide or {0 potassium iodide.

Step 2

The compound [II] or its salt can be prepared by subjecting a compound [VI] or its salt to elimination reaction of the amino-protective group.

This elimination reaction can be carried out by a conventional manner, and the reaction mode [e.g. hydrolysis, reduction, etc.] and the reaction conditions [e.g. acid, base, catalyst, solvent, reaction temperature, etc.] of this reaction can be referred to those of the conventional elimination reaction of the amino-protective group.

Q 5s

Process B

Step 1 ’

The compound [IX] or its salt can be prepared by reacting a compound [VII] or its salt with a compound (VIII].

Suitable salts of the compounds [VII] and [IX] can be referred to the ones as exemplified for the compound {III].

This reaction is so-called Wittig reaction, and the reaction mode and reaction conditions can be referred to

- 20 ~- those of the conventional Witting reaction.

Step 2

The compound [III] or its salt can be prepared by subjecting a compound [VIII] or its salt to elimination reaction of the carboxy-protective group.

This elimination reaction can be carried out by a conventional manner, and the reaction mode [e.q. hydrolysis, reduction, etc.] and the reaction conditions [e.g. acid, base, catalyst, solvent, reaction temperature, etc.] of this reaction can be referred to those of the () conventional elimination reaction of the carboxy : protective group.

The compounds obtained by the above Processes 1, 2, 3, A and B can be isolated and purified by a conventional method such as pulverization, recrystallization, column chromatography, reprecipitation or the like.

It is to be noted that each of the obizcr compound [I] and the starting compounds may include one or more stereoisomer due to asymmetric carbon atom(s) and/or carbon-carbon double bond (i.e. 7-isomer and E-isomer), and all such isomers and mixture thereof are included : within the scope of this invention.

Q 25 The new indolylpiperidine compound [I] and pharmaceutically acceptable salts thereof possess ; antiallergic activity and are useful for a therapeutic : treatment or prophylaxis of allergic disease such as allergic asthma, allergic rhinitis, allergic conjunctivitis chronic urticaria, or the like.

The compound (I] and a pharmaceutically acceptable salt thereof of this invention can be used in the form of conventional solid, semisolid or liquid pharmaceutical preparations in admixture with conventional organic or inorganic carriers or excipients suitable for oral,

r - 21 -~- parenteral or external application. The active ingredients may be admixed with conventional, nontoxic, pharmaceutically acceptable carriers having the form of, for example, tablets, pellets, capsules, patches, suppositories, solutions, emulsions or suspensions or : any other form suitable for use. Usable carriers are not limited to any particular species. Thus, conventional carriers such as water, glucose, lactose, gum arabic, gelatin, mannitol, starch paste, magnesium i 10 trisilicate, talc, corn starch, keratin, colloidal silica, potato starch and urea and other carriers 0) suitable for the manufacture of solid, semisolid or liquid preparations can be used. Furthermore, auxiliaries, stabilizers, thikening agents and colorants as well as aromas may be added.

The dose or therapeutically effective amount of the object compounds [I] of this .invention may vary depending on the age and symptoms of each individual patient to be treated. Generally, the active ingredients are administered for disease treatment in a daily dose : : of about 0.1-100 mg/kg, preferably 0.1-10 mg/kg. . In order to illustrate the usefulness of the object compound [I], the pharmacological test data of

O some representative compounds of the compound [I] are shown in.the following.

Test Compounds

Compound A : 1-(4-{5- (4-Hydroxy-3-methoxyphenyl) - (2E, 4E) - 2,4-pentadienoylaminolbutyl}-4-(3-indolyl) - piperidine

Compound B : 1-[2-{5-(4-Hydroxy-3-methoxyphenyl) - (2E,4E) -2,4-pentadienoylaminol}ethyl]- 4-(3-indolyl) piperidine

Compound C : 1-[2-{5-(4-Hydroxy-3,5-dimethoxyphenyl)- (2E,4E)-2,4-pentadienoylamino}ethyl]- 4-(3~indolyl) piperidine

Compound D : 1-[{2-{5-(4-Acetoxy-3~-methoxyphenyl)- : (2E,4E)-2,4-pentadienoylamino}ethyl]-4-(3- indolyl) piperidine

Compound E : 1-[2-{5-(4-Acetoxy-3,5~-dimethoxyphenyl)- (2E,4E)-2,4-pentadienoylamino}ethyl]-4-

O (3-indolyl) piperidine \

Compound F : 1-[2-{5-(3,5-Dichloro-4-hydroxyphenyl)- (2E,4E)-2,4-pentadienoylaminolethyl]-4- (3-indolyl) piperidine

Test 1

Antagonistic action on anaphylactic asthma in guinea pigs

Male Hartley-strain guinea pigs weighing 305-400 g were used. These animals were sensitized by intravenous injection of 0.5 ml/animal of rabbit antiserum to egg-white albumin (PCA antibody titer 4,000). After 24 hours, the animals were housed

O . individually in 5.3-liter plastic chambers. Using a commercial sprayer, a 5% egg-white albumin solution was - sprayed in the form of an aerosol into each chamber at a rate of 0.16 ml/min for 2 minutes. Thirty minutes prior to the spraying of the egg-white albumin solution, the test compound was administered orally in varied concentrations. Each dosed group consisted of 5 animals. The prophylactic effect to anaphylaxis was expressed in terms of the EDg value determined on the basis of the number of guinea pigs which had survived for not less than 2 hours after antigen spraying for each administration concentration of the test compound.

i - 23 - i

The values thus obtained are given in the following table.

Test Results ~

Prophylactic Effect ED

Test d es _ Compoun (mg/kg) 50 \ A 0.5

C 0.5 : 10 Test 2 : Anti-SRS-A activity

C Peritoneal exudate cells were ccllected from : glycogen-injected SD rats and adjusted to 1 x 107 ! cells/ml with Tyrode's solution. One milliliter of the cell suspension was incubated with indomethacin (10 ug/m&) and each varied concentration of the test "compound for 10 minutes and, then, further incubated with Ca'T-ionophore (A23187, 1 ug/mf) for 10 minutes.

The supernatant was collected by centrifugation and the

SRS-A (slow-reacting substance of anaphylaxis) activity

So was determined in terms of contractility of the isolated guinea pig ileum in the presence of mepyramine, atropine and methysergide. ; OO : The results were expressed in terms of the 50% ~" 25 inhibitory concentration to SRS-A synthesis or release © from peritoneal exudate cells.

Test results : Inhibitory Concentration

Test Compound ICs (ug/md)

B 0.91

C 0.68

D 0.6 { 5 3 E 0.23 ; F 0.65 i

I

The following Preparations and Examples are given for the purpose of illustrating the present invention in more detail. -

Preparation 1

A mixture of 4-(3-indolyl)piperidine (7.88 gq),

N- (2-bromoethyl) phthalimide (10.0 g) and sodium hydrogen carbonate (3.64 g) in dry N,N-dimethylformamide (93 ml) was heated at 68-74°C for 4 hours. After cooling, the reaction mixture was poured into ice-water (1,000 ml).

The resulting precipitate was collected by filtration

C and washed with methanol to give 1-(2-phthalimidoethyl)- 4- (3-indolyl)piperidine (5.53 g).

NMR (DMSO-d, §) «+ 1.3-3.4 (11H, m), 3.77 (2H, t,

J=6.0Hz), 6.8-7.8 (5H, m), 7.89 (4H, m), 10.73 (1H, s)

MASS : 373 (M7), 213

Preparation 2 :

A mixture of 4-(3-indolvl)piperidine (7.47 gq), :

N- (3-bromopropyl) phthalimide (10.0 g) and sodium hydrogen carbonate (3.45 g) in dry N,li-dimethyl formamide (88 ml) was heated at 70°C for 2 hours. After cooling, the a . reaction mixture was poured into water (880 ml) and

OL 25 extracted with a mixture of chloroform and methanol : (lo:1 V/V). The organic layer was washed with a . saturated sodium chloride solution and dried over magnesium sulfate. The solvent was distilled off and the residue was subjected to column chromatography on silica gel (290 g) and eluted with a mixture of chloroform and methanol (20:1 V/V). The fractions containing the object compound were combined and concen- trated under reduced pressure. The residue was triturated with diethyl ether to give pale yellow crystals of 1-(3-phthalimidopropyl)-4-(3-indolyl)-

IR (Nujol) : 3360, 1770, 1704, 1040, 735, 712 —

NMR (DMSO-d. §) : 1.0-3.1 (13H, m), 3.67 (2H, t, ! J=6.0Hz), 6.8-7.6 (5H, m), 7.6-8.0 (4H, m), 10.63 (1H, s)

Preparation 3 1- (4-Phthalimidobutyl) -4- (3-indolyl) piperidine was obtained according to a similar manner to that of

Preparation 2.

IR (Nujol) =: 3400-3300 (broad), 1770, 1700 (broad) cm

CQ

Preparation 4

A mixture of 1- (2-phthalimidoethyl)-4-(3-indolyl)- piperidine (6.3 g) and hydrazine monohydrate (2.2 g) in ethanol (250 ml) was refluxed for 70 minutes. After cooling, the reaction mixture was filtered and the filtrate was concentrated under reduced pressure. The residue was treated with 5% sodium hydroxide solution (300 ml) and extracted with ethyl acetate (300 ml).

The organic layer was washed with a saturated sodium chloride solution and dried over magnesium sulfate.

The evaporation of solvent gave 1- (2-aminoethyl)-4- ( (3-indolyl) piperidine (3.74 9). . ~ 25 IR (Nujol) : 3350, 1596, 953, 733 em

NMR (CDCl, 6) : 1.5-3.4 (15H, m), 6.8-7.8 (SH, m), : 8.5.(lH, br s)

MASS : 243 (M7), 213

Preparation 5

The following compounds were obtained according to a similar manner to that of Preparation 4. (1) 1- (3-Aminopropyl)-4- (3-indolyl)piperidine

IR (Nujol) : 3360, 3150, 1377, 1225 em!

A

. - 26 -

NMR (DMSO-d, §) : 1.3-3.2 (17H, m), 6.7-7.7 (5H, m), 10.67 (1H, s) (2) 1-(4-Aminobutyl)-4-(3-indolyl)piperidine

IR (Nujol) : 3390, 3150, 1110, 897, 736 cm ©

NMR (DMSO-d, §) =: 1.0-3.2 (19H, m), 6.7-7.6 (5H, m), 10.67 (lH, s)

Preparation 6

A mixture of 4-hydroxy-3,5-dimethylbenzaldehyde (5 g), N,N-diisopropylethylamine (6.9 ml), (2-methoxy- ( ethoxy)methylchloride (4.26 ml) and 1,2-dichloroethane (65 ml) was refluxed for 5 hours. The reaction mixture was washed with water and dried over magnesium sulfate.

After removal of the solvent, the residue was subjected to column chromatography on silica gel and eluted with a mixture of n-hexane and ethyl acetate (8:2 V/V). The fractions containing the object compound were combined and concentrated under reduced pressure to give 4-{(2- methoxyethoxy)methoxy]-3,5-dimethylbenzaldehyde (6.54 g).

IR (neat) : 2900, 1690, 1600, 1130, 1100, 960, oo 740 cmt

NMR (CDCly, §) + 2.30 (6H, s), 3.32 (3H, s), . : 3.75, 4.0 (each 2H, m), 5.19 (2H, m), :

O 25 7.60 (2H, s), 9.93 (1H, s) : Preparation 7

The following compounds were obtained according to a similar manner to that of Preparation 6. (1) 3,5-Diisopropyl-4-((2-methoxyethoxy)methoxy]- benzaldehyde

IR (Nujol) : 2950, 1690, 1595, 1585, 955 — (2) 4-[(2-Methoxyethoxy)methoxy]-3-methylbenzaldehyde

IR (neat) : 2950, 1690, 1600, 1590, 980 emt

NMR (CDCL 5, §) : 2.31 (3H, s), 3.38 (3H, s), - 3.6,3.8 (each, 2H, m), 5.41 (2H, s), 7.15-7.85 (3H, m), 9.90 (lH, s) (3) 3-Chloro-4-[ (2-methoxyethoxy)methoxy|benzaldehyde

IR (neat) : 1700, 1595, 1570, 950 emt

NMR (CDCl 4, 8) 3.30 (3H, m), 3.6, 3.8 (each, 2H, m), 5.53 (2H, s), 7.2-7.9 (3H, m), 9.88 (lH, s) (4) 3,5-Dichloro-4-[ (2-methoxyethoxy)methoxy]benzaldehyde () IR (neat) : 2900, 1705, 1590, 1560, 920, 810 cm ©

NMR (CDC15, §) + 3.4 (3H, s), 3.6, 4.1 (each 2H, m), 5.38 (2H, s), 7.82 (2H, s), 9.85 (lH, s) (5) 3-Methoxy-2-[ (2-methoxyethoxy) methoxy Jbenzaldehyde

IR (heat) : 1690, 1585, 950, 859, 785, 750 em!

NMR (CDCly, §) : 3.40 (3H, s), *.6, 3.9 (each 2H, m), 3.95 (3H, s), 5.38 (2H, 3), 7.2-7.6 (3H, m), 10.53 (lH, s)

MASS (m/e) : 240 (M7), 89, 59 (6) 3,5-Di-tert-butyl-4-[(2-methoxyethoxy)methoxy]-

CY : benzaldehyde IN = 25 IR (neat). : 1695, 1595, 945 cm

Preparation 8

To a stirred suspension of 60% sodium hydride (1.01 g) in dry tetrahydrofuran (60 ml), 80% triethyl 4-phosphonocrotonate (6.57 g) was added dropwise below 10°C under an inert atmosphere. After being stirred for 30 minutes, a solution of 4-[(2-methoxyethoxy)methoxy]- 3,5-dimethylbenzaldehyde (5.0 g) in dry tetrahydrofuran (50 ml) was added thereto below 10°C. After stirring for 2 hours, the reaction mixture was concentrated under

: I. - 28 - i reduced pressure. The residue was dissolved in ethyl acetate (100 ml), washed with a saturated aqueous solution oo. of sodium chloride and dried over magnesium sulfate. The solvent was distilled off and the residue was subjected to column chromatography on silica gel (130 g) and eluted with a mixture of n-hexane and ethyl acetate (7:3 V/V).

The fractions containing the object compound were combined and concentrated under reduced pressure to give a syrup of ethyl 5-[4-{(2-methoxyethoxy)methoxy}-3,5-dimethyl- phenyl]- (2E,4E)-2,4-pentadienoate (5.28 g). 0 IR (neat) : 2950, 1710, 1620, 1600, 970, 865 em”!

Preparation 9

The following compounds were obtained according to a similar manner to that of Preparation 8. (1) Ethyl 5-[3,5-diisopropyl-4-{ (2-methoxyethoxy)methoxy}- phz.y11-(2E,4E)-2,4-pentadienocate

IR (Fujol) : 1710, 1625, 1595, 965, 870 emt

NMR (CDC15, §) «+ 1.25 (12H, 4, J=8Hz), 1.31 (3H, t,

J=8Hz), 3.45 (2H, sextet, J=8Hz), 3.43 (3H, s), 3.7, 4.0 (each 2H, m), 4.25 (2H, q, J=8Hz), 5.03 (24, s), 6.0 (1H, 4, J=15Hz), 6.8-7.7 . (5H, m)

O 25 MASS (m/e) : 362 (M'), 89, 59 (base) (2) Ethyl 5-(4-{(2-methoxyethoxy)methoxy}-3-methylphenyl]- (2E,4E)-2,4-pentadiencate

NMR (cpcl,, §) + 1.31 (3H, t, J=8Hz), 2.25 (3H, s), 3.35 (34, s), 3.7, 3.9 (each, 2H, m), 4.25 (2H, g, J=8Hz), 5.31 (2H, s), 5.95 (1H, 4, J=15Hz), 6.7-7.7 (6H, m)

MASS (m/e) : 320 (M), 276, 89, 59 (3) Ethyl S-[3-chloro-4-{(2-methoxyethoxy)methoxy}-

. _ 2g - phenyl]-(2E,4E)-2,4-pentadienocate

IR (neat) : 2900, 1710, 1630, 1600, 1055, 980 emt ~ NMR (CDCL,, §) «+ 1.31 (3H, t, J=8Hz), 3.35 (3H, s)., 3.7, 3.9 (each 2H, m), 4.28 (2H, gq, J=8Hz), 5.33 (2H, s), 5.97 (lH, d, J=15Hz), 6.7-7.7 (6H, m) (4) Ethyl 5-(3,5-dichloro-4-{ (2-methoxyethoxy)methoxy}- _phenyl]-(2E,4E)-2,4-pentadienocate mp : 67-69°C (recrystallized from a mixture of toluene and ethyl acetate (8:1))

O IR (Nujol) : 1710, 1630, 1545, 1000, 925, 860, 800 —

NMR (CDClg, §) + 1.30 (3H, t, J=8Hz), 3.38 (3H, s). 3.6, 4.1 (each 2H, m), 4.23 (2H, gq, J=8Hz), 5.29 (2H, s), 6.03 (1H, 4, J=15Hz), 6.6-7.7 (5H, m)

MASS (m/e) : 376 (M+2), 375 (M+1), 374 (M%), 89 (base) : . 20 (5) Ethyl 5-[3-methoxy-2-{ (2-methoxyethoxy)methoxy}- phenyl]-(2E,4E)~2,4-pentadienoate mp : 48-49°C (recrystallized from a mixture of : Co } n-hexane and diisopropyl ether) ‘ “7 2s IR (Nujol) : 1720, 1623, 1000, 945, 850 emt ; NMR (CDCl, 6) : 1.35 (3H, t, J=7Hz), 3.4 (3H, s), ] : 3.6, 3.9 (each 2H, m), 3.86 (3H, s), 4.27 ’ (2H, gq, J=7Hz), 5.25 (2H, s), 6.03 (1H, 4,

J=15Hz), 6.6-7.7 (6H, m) (6) Ethyl 5-[4-methoxy-3-{(2-methoxyethoxy)methoxy}- phenyll-(2E,4E)-2,4-pentadiencate

IR (neat) : 1710, 1625, 1600, 1000 cm

NMR (CDCl 5, §) + 1.36 (3H, t, J=7Hz), 3.4 (3H, s), 3.6, 3.9 (each 2H, m), 3.90 (3H, s),

i - 30 - ! 4.25 (28, q, J=7Hz), 5.31 (2H, s), 5.98 (lH, d,

J=15Hz), 6.6-7.8 (6H, m) (7) Ethyl 5-[3,5-di-tert-butyl-4-{ (2-methoxyethoxy)- methoxy }phenyl]- (2E,4E)-2,4-pentadienoate

IR (neat) : 1710, 1625 —

Preparation 10

To a stirred solution of ethyl 5-[4-{ (2-methoxy- i 10 ethoxy) methoxy }-3,5-dimethylphenyl]- (2E,4E)-2, 4- i pentadienoate (5.28 g) in methanol (55 ml) was added a ; O solution of sodium hydroxide (6.32 g) in water (18 ml) i below 20°C. After being stirred for an hour, the reaction mixture was concentrated under reduced pressure. The residue was dissolved in water (200 ml) and adjusted to pH 4 with 10% hydrochloride solution. The resulting precipitate was collected by filtration and washed with : water to give yellowish powder of 5-[4-{ (2-metlicxyethoxy)- methoxy }-3,5-dimethylphenyl]-(2E,4E)-2,4-pentadienoic acid (4.13 g). mp : 88-91°C

IR (Nujol) : 2650, 1675, 1615, 1595, 1000, 970, 860 —

O NMR (CDCl,, 6) : 2.30 (6H, s), 3.43 (3H, s), “25 3.7, 4.0 (each 2H, m), 5.05 (2H, s), 5.95 (1H, d, J=15Hz), 6.75-7.8 (5H, m), 10.25 (LH, m)

MASS (m/e) : 306 (M'), 89 (base)

Preparation 11

The following compounds were obtained according to a similar manner to that of Preparation 10. (1) 5-[3,5-Diisopropyl-4-{ (2-methoxyethoxy)methoxy}- phenyl]-(2E,4E)-2,4-pentadienoic acid mp : 96-113°C

IR (Nujol) : 2600, 1685, 1615, 1595, 1100, 1080, ‘970 em”?

NMR (CDC1,, §) «+ 1.25 (12H, 4, J=8Hz), 3.45 (2H, * sext, J=8Hz), 3.43 (3H, s), 3.7, 4.0 (each 2H, m), 5.03 (2H, s), 6.0 (1H, &, J=15Hz), 6.8-7.8 (5H, m), 10.13 (1H, m)

MASS (m/e) : 362 (M'), 89, 59 (base) (2) 5-[4-{(2-Methoxyethoxy)methoxy}-3-methylphenyl]- (2E,4E)-2,4-pentadienoic acid ] mp : 117-119°C ( IR (Nujol) : 2600, 1670, 1600, 1000, 930 cm '

NMR (CDCl 4, §) «+ 2.26 (3H, s), 3.30 (3H, s), 3.6, 3.9 (each, 2H, m), 5.32 (2H, s), 5.98 (14, 4, J=15Hz), 6.7-7.8 (6H, m), 8.7 (lH, m) (3) 5-[3-Chloro-4-{(2-methoxyethoxy)methoxy}phenyl]- (2E,4E)-2,4-rzrtadienoic acid mp : 130-135°C :

IR (Nujol) : 2600, 1680, 1615, 1590, 1050, 995 cm » . NMR (CDCly, 6) : 3.30 (3H, s), 3.6, 3.9 (each 2H, m), 5.38 (ZH, s), 6.01 (1H, 4d, J=15Hz), 6.7-7.7 (6H, m), 9.7 (lH, m) (J (4) 5-[3,5-Dichloro-4-{(2-methoxyethoxy)methoxy}- . phenyl]- (2E,4E)-2,4-pentadienoic acid mp : 116-120°C

IR (Nujol) : 2600, 1690, 1630, 990, 905, 805 cm ©

NMR (cpCly, 8) + 3.40 (3H, s), 3.6, 4.1 (each 2H, m), 5.29 (2H, s), 6.05 (1H, 4, J=15Hz), 6.7-7.7 (5H, m), 9.65 (lH, br)

MASS (m/e) : 348 (M+2), 346 (M'), 89, 59 (base) (5) 5-[3-Methoxy-2-{(2-methoxyethoxy)methoxy}phenyl]- (2E,4E)-2,4-pentadienoic acid

. Cay

IR (Nujol) : 2600, 1690, 1610, 1050, 955 —

NMR (CDC1 5, §) «+ 3.33 (3H, s), 3.5, 3.8 (each 2H, m), 3.80 (3H, s), 5.15 (2H, s), 5.93 (1H, 4d,

J=15Hz), 6.7-7.7 (6H, m), 9.5 (lH, br) (6) 5-[4-Methoxy-3-{(2-methoxyethoxy)methoxy}phenyl]- (2E,4E)~-2,4-pentadienoic acid mp : 121-125°C

IR (Nujol) : 2600, 1670, 1620, 1590 cmt

NMR (CDC14, §) : 3.35 (3H, s), 3.55, 3.90 (each 2H, m), 3.86 (3H, s), 5.30 (2H, s), 5.92 (lH,

C d, J=15Hz), 6.7-7.7 (6H, m), 10.2 (1H, br) (7) 5-[3,5-Di-tert-butyl-4-{ (2-methoxyethoxy)methoxy}- phenyl]-(2E,4E)-2,4-pentadienoic acid

IR (Nujol) : 2650, 1680, 1620, 970 cm ©

NMR (CDCl, §) + 1.46 (18H, s), 3.42 (3H, s), 3.66, 3.96 (each 2H, m), 5.0 (2H, s)., 5.97 (1H, 4, J=15.5Hz), 6.6-7.7 (5H, m), ‘ 9.2 (1H, br)

Example 1

To a stirred mixture of 3-[3-methoxy-4-{(2-methoxy-

GC ethoxy) methoxylphenyl]l- (E)-propenoic acid (1.75 g) and triethylamine (1.81 ml) in dry N,N-dimethylformamide (10 ml) was added slowly diphenyl phosphinic chloride (L.47 g) at ~10 to -15°C under an inert atmosphere.

After being stirred for 30 minutes, a solution of 1- (2-aminoethyl)~-4-(3-indolyl)piperidine (1.5 g) in dry

N,N-dimethylformamide (10 ml) was added slowly to the reaction mixture at -10°C. After being stirred for 1 hour at ambient temperature, the reaction mixture was poured into ice-water (200 ml) and extracted with chloroform (100 ml). The extract was washed with a saturated sodium chloride solution and dried over magnesium sulfate. The solvent was distilled off and the residue was subjected to column chromatography on silicagel (47 g) and eluted with a mixture of chloroform and methanol (10:1). The fractions containing the object compound were combined and concentrated under reduced pressure to give syrup of 1-[2-[3-[3-methoxy-4- { (2-methoxyethoxy) methoxy }phenyl]- (E) -propenoylamino]- ethyl]-4-(3-indolyl) piperidine (2.8 g). i NMR (CDC, §) : 1.6-3.3 (11H, m), 3.37 (3H, s), 3.55 (4H, m), 3.85 (2H, m), 3.89 (3H, s), 5.32 (2H, s), 6.35 (1H, d, J=15.0Hz),

O 6.52 (lH, br s), 6.9-7.8 (8H, m), 7.57 (1H, 4, J=15.0Hz), 8.25 (1H, br s)

Example 2

The following compounds were obtained according to a similar manner to that of Example 1. (1) 1-[2- [5-[3-Methoxy-4-{ (2-methoxyethoxy) methoxy }~ phenyl] - (2E,4E)-2,4-pentadienoylamino]ethyl]-4-(3- indolyl) piperidine

IR (Nujol) : 3300, 1660, 1260, 1092, 990, 744 cm!

NMR (CDC1 5, §) + 1.6-3.3 (11H, m), 3.35 (3H, s),

O 3.54 (4H, m), 3.84 (2H, m), 3.86 (3H, sj, 5.30 (2H, s), 6.07 (lH, d, 15.0Hz), 6.70-7.80 (12H, m), 9.30 (1H, s) .

MASS : 533 (M'),213 (2) 1- [3- [5- [ 3-Methoxy-4-{ (2-methoxyethoxy)methoxy!}~- phenyl]- (2E,4E)-2,4-pentadienoylamino]propyl}-4- (3-indolyl) piperidine

NMR (CDCl,, §) + 1.5-3.6 (15H, m), 3.36 (3H, s), 3.6 (2H, m), 3.87 (3H, s), 3.90 (2H, m), 5.35 (2H, s), 6.02 (lH, 4, J=14.4Hz), 6.6-7.9 (12H, m), 8.55 (1H, s)

(3) 1-[4-[5-[3-Methoxy-4-{ (2-methoxyethoxy)methoxy}- : phenyl]- (2E,4E)-2,4-pentadienoylamino]butyl]-4-(3- : ~~ indolyl)piperidine

IR (Nujol) : 3400, 3200 (broad), 1650, 1377, 1260 emt

NMR (CDC, §) + 1.3-3.4 (17H, m), 3.33 (3H, s), 3.55 (2H, m), 3.80 (5H, br s), 5.27 (2H, s), 6.11 (1H, d, J=15.0Hz), 6.5-8.0 (12H, m), : 9.23 (1H, s) : MASS : 561 (M7) ] 10 : (4) 1-[{2-{5-(3,4-Dimethoxyphenyl)-(2E,4E)-2,4-

O pentadienoylamino}ethyl]-4-(3-indolyl) piperidine mp : 196-198°C (recrystallized from ethanol)

IR (Nujol) : 3280, 1640, 1610, 1590, 1550, 1510 cm © 1s NMR (DMSO-d, §) + 1.4-3.5 (13H, m), 3.78 (3H, s), 3.81 (3H, s), 6.15 (1H, 4d, J=15.0Hz), 6.8-7.6 (11H, m), 7.99 (lH, br t), 10.75 (lH, br s)

Mass : 459 (M%), 213 ' 20 Elemental analysis : CogH33N30, : Calcd. : C 73.18, H 7.24, N 9.14

Found : C 73.84, H 7.42, N 8.72 : ~ (5) 1-[2-{5-(3,4,5-Trimethoxyphenyl)-(2E,4E)-2,4- & 25 pentadienoylamino}ethyl]~-4-(3-indolyl) piperidine mp : 86-100°C j IR (Nujol) : 3250, 1650, 1610, 1580 cm ©

NMR (DMSO-d, 8) : '1.4-3.6 (13H, m), 3.70 (3H, s), 3.83 (6H, s), 6.19 (1H, 4, J=15.0Hz), 6.7-7.7 (10H, m), 8.02 (lH, br t), 10.74 (1H, br s) : MASS : 489 (M') 289, 213

Elemental analysis : C,gH45N40,"3/4H,0 calcd. : C 69.23, H 7.31, N 8.35

Found : CC 69.38, H 7.08, N 8.40

- 35 = (6) 1-[2-{3-(4-Hydroxy-3—-methoxyphenyl)-(E)-propenoyl- aminol}ethyl]-4-(3-indolyl) piperidine mp : 115-135°C .

IR (Nujol) : 3300 (broad), 1655, 1588, 1512 cm © (7) 1-[2-{5-(4-Hydroxy-3-methoxyphenyl)-(2E,4E)-2,4- pentadienoylaminolethyl]-4-(3-indolyl) piperidine mp : 115-131°C

IR (Nujol) : 3330 (broad), 1660, 1377 cm * (8) 1-[3-{5-(4-Hydroxy-3-methoxyphenyl)-(2E,4E)-2,4-

O pentadienoylamino}propyl]-4-(3-indolyl)piperidine mp : 150-170°C

IR (Nujol) : 3400, 3200 (broad), 1638, 1580 cm © (9) 1-(4-{5-(4-Hydroxy-3-methoxyphenyl)-(2E,4E)-2,4- pentadienoylamino}butyl]-4-(3-indolyl)piperidine mp : 150-170°C

IR (Nujol) : 3200 (broad), 1640, 1580, 1270, 735 emt 20 . (10) 1-[2-[5-[3,4-Bis{ (2-methoxyethoxy)methoxy}phenyl]- (2E,4E)-2,4-pentadienoylamino]ethyl]~-4-(3-indolyl)- piperidine

CIP

This compound was used as a starting compound of

Example 7-(4) without purification. (11) 1-{2-([5-[3,5-Dimethoxy-4-{ (2-methoxyethoxy)methoxyl}- phenyl]-(2E,4E)-2,4~-pentadienoylamino]ethyl]-4-(3- indoyl) piperidine

IR (Nujol) : 3300, 1550, 1610, 1580, 1125, 990, 960, 845, 745 cm (12) 1-(3-(5-[3,5~-Dimethoxy-4-{(2-methoxyethoxy)methoxy}- phenyl]-(2E,4E)-2,4-pentadienoylamino]lpropyl]-4-

IR (neat) : 3300, 3000, 2990, 1650, 1615, 1580, 1130, 990, 960, 850 emt (13) 1-[4-(5-[3,5-Dimethoxy-4-{ (2-methoxyethoxy)methoxy}- phenyl]- (2E, 48) -2,4-pentadienoylaminolbutyl]-4- (3- indolyl)piperidine

IR (neat) : 2900, 1650, 1610, 1580, 1550, 1120, 960, 850, 740 cm j 10 (14) 1-[2-[5-[4-{(2-Methoxyethoxy)methoxy}-3,5- : dimethylphenyl]- (2E,4E)-2,4-pentadienoylamino]ethyl]-

O 4-(3-indolyl)piperidine mp : 163-164°C (recrystallized from ethyl acetate)

IR (Nujol) : 3450, 3360, 1645, 1615, 990, 970 —

NMR (DMSO-d, 6) : 1.5-2.3 (6H, m), 2.34 (6H, s), 2.5-3.1 (7H, m), 3.25 (3H, s), 3.5, 3.8 : (each 2H, m), 5.05 (2H, s), 6.15 (1H, d, J=15Hz), 6.8-7.7 (10H, m), 8.03 (1H, m), 10.7 (1H, m)

MASS (m/e) : 531 (M'), 213 (base) oo (15) 1-[2-(5-[3,5-Diisopropyl-4-{ (2-methoxyethoxy)- : methoxy }phenyl]-(2E,4E)-2,4-pentadienoylamino]- ethyl]-4-(3-indolyl) piperidine ; : IR (neat) : 1660, 1650, 1615, 970 cm ©

O 2 (16) 1-[2-[5-[4-{ (2-Methoxyethoxy)methoxy}-3-methyl- phenyl]-(2E,4E)-2,4-pentadienoylamino]ethyl]-4-(3- indolyl)piperidine mp : 140-144°C

IR (Nujol) : 3470, 3280, 1640, 1610, 1595, 1000, 980 cm *

NMR (CDCl,, 8) : 1.6-3.2 (13H, m), 2.25 (3H, s), : 3.38 (3H, s), 3.6, 3.8 (each, 2H, m), 5.32 (2H, s), 5.96 (lH, d, J=15Hz), 6.2-7.8 (llH, m), 8.25 (1H, m)

SER FY on 7 oN nN ee a Lo 31 £1 om oem)

(17) 1-[2-[5-[3-Chloro-4-{ (2-methoxyethoxy)methoxy}- phenyl] -(2E,4E)-2,4-pentadienoylamino]ethyl]-4- (3-indolyl)piperidine

IR (Nujol) : 3450, 3300, 1645, 1610, 1050, 990 a

NMR (DMSO-d, §) + 1.5-2.5 (6H, m), 2.8-3.2 (7H, m), 3.65 (3H, s), 3.6, 3.8 (each 2H, m), 5.39 (2H, s), 6.10 (1H, 4d, J=15Hz), 6.8-7.9 (11H, m), 8.05 (1H, m), 10.75 (lH, m)

MASS (m/e) : 537, 213 (base) (18) 1-[2-{5-(3,4-Dihydroxyphenyl)-(2E,4E)-2,4- ® pentadienoylaminolethyl]-4- (3-indolyl) piperidine

IR (Nujol) : 3400, 3350, 1650, 1585, 1520 —

MASS (m/e) : 431 (M'), 213 (base) (19) 1-[2-{5-(4-Hydroxy-3,5~dimethoxyphenyl)-(2E,4E)- 2,4-pentadienoylamino}ethyl]-4-(3-indolyl)piperidine

IR (Nujol) : 3420, 1665, 1650, 1520, 1590, 1530, 1515, 1120 cm *

MASS (m/e) : 475 (M'), 213 (20) 1-([4-(5-(4-Hydroxy-3,5-dimethoxyphenyl)-(2E,4E)~- 2,4-pentadienoylamino}butyl]-4-(3-indolyl) piperidine - IR (Nujol) : 3250, 1640, 1600, 1540, 1510, 1130,

OC 2 | 1110, 810 cm 1 (21) 1-[3-{5-(4-Hydroxy-3,5-dimethoxyphenyl) - (2E, 4E) - 2,4-pentadienoylamino}lpropyl]-4-(3-indolyl) piperidine

IR (Nujol) : 3420, 1658, 1610, 1575, 1550, 1510, 1120 cm

MASS (m/e) : 489 (M'), 239, 233, 213 (base), 197 (22) 1-[{2-{5-(4-Acetoxy-3-methoxyphenyl)-(2E,4E)-2,4- pentadienoylamino}lethyl]—-4-(3-indolyl)piperidine

IR (Nujol) : 3440, 3230, 1760, 1655, 1620, 1560,

TENE ~en

MASS (m/e) : 487 (M'), 213 (base) . (23) 1-[2-{5-(3-Methoxy~4-propionyloxyphenyl)- (2E,4E)~- 2,4~-pentadienoylamino}ethyl]-4~(3-indolyl)piperidine

IR (Nujol) : 3430, 3250, 3060, 1750, 1655, 1620, 1560 cm ©

MASS (m/e) : 501 (M'), 213 (base) (24) 1-{2-{5- (4-Ethoxycarbonyloxy-3,5-dimethoxyphenyl)- (2E,4E)-2, 4-pentadienoylamino}tethyl]-4- (3-indolyl)- piperidine @ IR (Nujol) : 3360, 3300, 1750, 1640, 1590, 1130, : 1000, 735 cm ©

MASS (m/e) : 547 (M'), 228, 213 (base) (25) 1-{4-{5- (4-Ethoxycarbonyloxy-3,5-dimethoxyphenyl)- (2E,4E)-2,4-pentadienoylamino}butyl]-4-(3-indolyl)- pipecidine

IR (Nujol) : 3380, 3250, 1750, 1655, 1620, 1595, ‘ 20 1555, 1130, 1050, 1000, 735 —

MASS (m/e) : 575 (M'), 531, 503, 285, 233, 213 (base)

O : (26) 1-([2-{5-(4-Hydroxy-3,5-dimethylphenyl)- (2E, 4E)- 2,4-pentadienoylaminotethyl]-4-(3-indolyl)- : piperidine

IR (Nujol) : 3300, 1640, 1590, 1545, 990, 860 cm

MASS (m/e) : 443 (M7), 213 (base) (27) 1-[2-{5-(4-Hydroxy-3,5-diisopropylphenyl)- (2E,4E)- 2,4-pentadienoylamino}ethyl]~4-.3-indolyl)- piperidine

IR (Nujol) : 3400, 3300, 1650, 1630, 1585, 995, 870 em” 1

MASS (m/e) : 499 (M'), 226, 213 (base)

(28) 1-{2-{5-(4-Hydroxy-3-methylphenyl)-(2E,4E)-2,4~- pentadienoylaminolethyl]-4-(3-indolyl) piperidine

IR (Nujol) : 3200, 1640, 1575, 1550, 1000 em!

MASS (m/e) : 429 mt, 213 (base) (29) 1-[2-{5-(3-Chloro~-4-hydroxyphenyl)-(2E,4E)-2, 4~- pentadienoylaminolethyl]-4-(3-indolyl) piperidine

IR (Nujol) : 3420, 1650, 1590, 1000 cm ©

MASS (m/e) : 449 (M7), 213 (base) (30) 1-(2-{5-(4-Acetoxy-3,5-dimethoxyphenyl)- (2E,4E)- () 2,4-pentadienoylamino}tethyl]-4-(3-indolyl)piperidine

IR (Nujol) : 3380, 3320, 1755, 1650, 1620, 1595, 990, 745 cmt

MASS (m/e) : 517 (M'), 213 (base) (31) 1-[2-([5-[3,5-Dichloro-4-{ (2-methoxyethoxy) methoxy }- phenyl]-(2E,4E)-2,4-pentadienoylaminojethyl}-4- : (3-indolyl)piperidine

IR (neat) : 1655, 1610, 995 cm © (32) 1-[2-[5- [3-Methoxy-2-{ (2-methoxyethoxy) methoxy }- phenyl]-(2E,4E)~-2,4+pentadienoylamino]ethyl]-4- (3~-indolyl) piperidine : : O 25 IR (neat) : 1650, 1610, 1000, 960 em™1 (33) 1-{2-[5-[4-Methoxy-3-{ (2-methoxyethoxy)methoxy}- phenyl]l-(2E,4E)-2,4-pentadienoylamino]ethyl]-4- (3-indolyl) piperidine mp : 135-136°C (recrystallized from ethyl acetate)

IR (Nujol) :« 3260, 1640, 1615, 1595, 1550, 1510 —

NMR (DMSO-d, §) « 3.75 (3H, s), 5.23 (2H, s), 6.11 (14, 4d, J=15Hz), 6.7-7.6 (11H, m), 7.96 (1H, t 1like), 10.7 (1H, br)

MASS (m/e) : 533, 445, 333, 213 (base)

jo — ee PU 1 .0 - 1(34) 1-[2-[5-[3,5-Di-tert-butyl-2-{ (2-methoxyethoxy)- methoxy }phenyl]-(2E,4E)-2,4~-pentadienoylamino]- , ethyl]-4-(3-indolyl) piperidine mp : 98-103°C (recrystallized from ethanol)

IR (Nujol) : 3300, 1650, 1600, 970 cm *

NMR (CDCl,, 6) : 1.42 (18H, s), 1.6-2.3 (6H, m), 1 2.53 (2H, t, J=7Hz), 2.8 (3H, m), 3.35 (3H, s), 3.5 (2H, m), 3.66, 3.96 (each 2H, m), 4.93 (2H, s), 5.95 (1H, d, J=15.5Hz), 6.17 (lH, t like), 6.6-7.7 (10H, m), 8.2 (lH, s)

O (35) 1-[2-{5-(3,5-Di-tert-butyl-4-hydroxyphenyl)- (2E,4E)- : 2, 4-pentadienoylamino}ethyl]-4-(3-indolyl) piperidine i IR (Nujol) : 3550, 3300, 3230, 1650, 1610, 1590, 1000 cmt

MASS (m/e) : 527 (M'), 226, 213 ! i (36) 1l-[2-(5-(3,5-Dichloro-4-hydroxyphenyl)~ (25,47) ~ ; 2,4-pentadienoylaminol}ethyl]-4-(3-indolyl)}- piperidine : MASS (m/e) : 485 (M+2), 483 (M'), 213 (base) ; (37) 1-[2-{5-(2-Hydroxy-3-methoxyphenyl)- (2E,4E)-

O 2,4-pentadienoylamino}lethyl]-4-(3-indolyl) piperidine ; 25 IR (Nujol) : 3400, 3240, 1650, 1605, 1600, 1530, 1090, 1005 cm } MASS (m/e) : 445 (M7), 226, 213 (base) (38) 1-({2-{5-(3-Hydroxy-4-methoxyphenyl)- (2E,4E)-2,4- pentadienoylaminolethyl]-4-(3-indolyl)piperidine

IR (Nujol) : 3350, 1650, 1615, 1590 cm ©

MASS (m/e) : 445 (M'), 213 (base) (39) 1-(2-({5-{3,4-bis (Ethoxycarbonyloxy)phenyl}-(2E,4E)- 2,4-pentadienoylamino]ethyl]-4-(3-indolyl) piperidine

_ a - :

IR (Nujol) : 3500, 3350, 1775, 1650, 1620, 1000 —

MASS (m/e) : 529 M"-46), 457, 285 (base), 213

Example 3

To a solution of 1-[2-[5-[3,5-di-tert-butyl-4-{(2- methoxyethoxy)methoxyl}phenyl]- (2E,4E)-2,4-pentadienoyl- amino]ethyl]l-4- (3-indolyl)piperidine (0.5 g) in methanol (5 ml) was added dropwise methanesulfonic acid (0.26 ml) at 18-25°C. After 2 hours the reaction mixture was adjusted to pH 7.5 with 2N-sodium hydroxide and then poured into saturated sodium bicarbonate solution (50 ® ml). The resulting precipitate was collected and washed with water. The precipitate was subjected to column chromatography on silica gel and eluted with a mixture of chloroform and methanol (20:1, V/V). The fractions containing the object compound were combined and concentrated under reduced pressure. The residue was recrystallized from i,4-dioxane, to give white crystals of 1-(2-{5-(3,5-di-tert-butyl-4~hydroxyphenyl)~- (2E,4E)- 2,4-pentadienoylaminolethyl]}-4-(3-indolyl)piperidine (0.28 qg). mp : 108-115°C

IR (Nujol) : 3550, 3300, 3230, 1650, 1610, 1590,

O : 1000 cmt

NMR (CDCly, 6) : 1.43 (18H, s), 1.6-2.3 (6H, m), 2.53 (2H, t, J=7Hz), 2.7-3.2 (3H, m), 3.45 (2H, © m), 5.33 (1H, s), 5.93 (1H, 4, J=15.5Hz), 6.15 (1H, t like), 6.65-7.7 (10H, m), 8.16 (lH, s)

MASS (m/e) : 527 (M'), 226, 213

Example 4

To a stirred solution of 1-[2-[5-{3,5~dimethoxy-4- - {(2-methoxyethoxy)methoxylphenyl]-(2E,4E)~-2,4~ pentadienoylaminolethyl]-4-(3-indolyl)piperidine (10.0 g)

in methanol (100 ml) was added slowly methanesulfonic acid (2.3 ml) at ambient temperature. After stirring for 2 hours, the reaction mixture was adjusted to pH 7.2 with aqueous 2N sodium hydroxide solution, and poured into a solution of 4.5 g of sodium bicarbonate in 500 ml of water. After stirring for 30 minutes, the resulting precipitate was collected by filtration and washed with 100 ml of water. The residue was subjected to column chromatography on silica gel and eluted with a mixture of : 10 chloroform and methanol. The fractions containing the object compound were combined and concentrated under

O reduced pressure. The residue was recrystallized from ethanol to give 1-[2-{5-(4-hydroxy-3,5-dimethoxypkenyl)- (2E,4E)-2,4-pentadienoylamino}ethyl]-4- (3-indolyl)- piperidine (6.69 gq). mp : 199-202°C (dec.)

IR (Nujol) : 3420, 1665, 1650, 1620, 1590, 1530, 1515, 1120 cm ©

NMR (DMSO-d, §) : 1.5-2.4 (7H, m), 2.7-3.5 (6H, m), 3.81 (6H, s), 6.15 (1H, 4, J=14Hz), 6.8-7.8 (10H, m), 8.0 (lH, t like), 8.68 (lH, m), : 10.75 (1H, s)

MASS (m/e) : 475 (M'), 213

G Elemental analysis : C,gH33N30,

Calcd. : C 70.71, H 6.99, N 8.83 ] Found : C 70.34, H 6.56, N 8.65

Example 5

A mixture of 1-[3-[5-[3,5-dimethoxy-4-{(2-methoxy- ethoxy)methoxy}phenyl]-(2E,4E)-2,4-pentadienoylamino]- propyl]-4-(3-indolyl)piperidine (1.67 g) and p-toluene- sulfonic acid monohydrate (0.64 g) in methanol (33 ml) was refluxed for 30 minutes under an inert atmosphere.

Upon cooling to ambient temperature, the mixture was added dropwise to an aqueous sodium carbonate solution.

~The resulting powder was subjected to column chromato- graphy on silica gel and eluted with a mixture of chloroform and methanol (10:1 V/V). The fractions containing the object compound were combined and concentrated under reduced pressure. The obtained residue was recrystallized from a mixture of ethanol and water (7:3 V/V) to give 1-[3-{5-(4-hydroxy-3,5- dimethoxyphenyl)- (2E,4E)-2, 4-pentadienoylaminol}propyl]- 4- (3-indolyl) piperidine (0.51 g). mp : 176-179°C (recrystallized from ethanol - water (8:2, V/V))

O IR (Nujol) : 3420, 1658, 1610, 1575, 1550, 1510, 1120 emt

NMR (DMSO-d, §) «+ 1.4-2.5 (9H, m), 2.6-3.5 (6H, m), 3.79 (6H, ss), 6.10 (lH, 4, J=15Hz), 6.7-7.7 (10H, m), 8.05 (1H, t like), 8.7 (lH, m), 10.72 (1H, s)

MASS (m/e) : 489 (M'), 239, 233, 213 (base), 197

Elemental analysis : C,gHasM50,

Calcd. : C 71.14, H 7.20, N 8.58

Found =: CC 70.79, H 7.12, N 8.57

Example 6 ’ ~ A mixture of 1-[2-[3-[3-methoxy-4-{ (2-“methoxyethoxy)-

CO 25 methoxy }phenyl]- (E) -propenoylamino]ethyl]-4- (3-indolyl)- piperidine (2 g) and p-toluenesulfonic acid monohydrate : (1.05 g) in methanol (40 ml) was refluxed for 30 minutes : under an inert atmosphere. After the solvent was removed under reduced pressure, the residue was treated with water (100 ml), adjusted to pH 10.0 with a sodium carbonate solution and extracted with ethyl acetate.

The extract was washed with a saturated sodium chloride solution and dried over magnesium sulfate. After removal of the solvent, the residue was subjected to column chromatography on silica gel (31 g) and eluted with a mixture of chloroform and methanol (8:1 V/V). The fractions containing the object compound were combined _and concentrated under reduced pressure to give 1-[2- {3- (4-hydroxy-3-methoxyphenyl) - (E) -propenoylamino}tethyl]- 4- (3-indolyl) piperidine (0.89 g). mp : 115-135°C

IR (Nujol) : 3300 (broad), 1655, 1588, 1512 —

NMR (DMSO-d, §) «+ 1.5-3.6 (14H, m), 3.83 (3H, s), 6.50 (lH, 4, J=15.0Hz), 6.7-7.7 (9H, m), : 7.83 (1H, br t), 10.70 (1H, s)

MASS : 419 (MT), 213 () Elemental analysis : C,gHyglN305 7 1/2H,0

Caled. : C 70.00,H 7.06, N 9.80

Found +: CC 70.18,H 6.92, N 9.85

Example 7

The following compounds were obtained according to similar manners to those of Caexples 3 to 6. (1) 1-[2-{5-(4-Hydroxy-3-methoxyphenyl)-(2E,4E)-2,4- pentadienoylamino}lethyl]-4-(3-indolyl) piperidine mp : 115-131°C

IR (Nujol) : 3330 (broad), 1660, 1377 —

O . NMR (DMSO-d, §) + 1.5-3.6 (13H, m), 3.82 (3H, s), 6.07 (1H, 4, J=15.0Hz), 6.6-7.6 (8H, m), oo 7.90 (1H, br t), 9.20 (1H, s), 10.68 (lH, s)

MASS : 445 (MT), 213

Elemental analysis : C,,H3 N305-1/2H,0

Caled. : C 71.34, H 7.10, N 9.24

Found : CC 71.15, H 6.87, N 9.19 (2) 1-(3-{5-(4-Hydroxy-3-methoxyphenyl)-(2E,4E)-2,4- pentadienoylamino}propyl]-4-(3-indolyl) piperidine mp : 150-170°C

IR (Nujol) : 3400, 3200 (broad), 1638, 1580 emt

NMR (DMSO-d, §) «+ 1.5-3.8 (15H, m), 3.86 (3H, s), 4.20 (lH, broad), 6.15 (lH, 4, J=14.0Hz), 6.6-7.8 (11H, m), 8.26 (lH, br s), 10.82 (1H, s)

MASS : 459 (M7), 213

Elementan analysis : C,gH43N304-1/2CHCL 3+ 1/2C, HOC, Hy calcd. : C 65.85, H 6.97, N 7.55

Found : C 65.67, H 7.18, N 7.87 (3) 1-[4-{5-(4-Hydroxy-3-methoxyphenyl)- (2E,4E)-2,4- pentadienoylamino}butyl]-4-(3-indolyl)piperidine mp : 150-170°C

O IR (Nujol) : 3200 (broad), 1640, 1580, 1270, 735 —

NMR (DMSO-d, §) + 1.2-3.7 (17H, m), 3.80 (3H, s), 6.07 (lH, 4, J=15.0Hz), 6.6-7.8 (11H, m), 8.10 (1H, s), 9.25 (lH, s), 10.82 (1H, s)

MASS : 473 (M7), 213

Elemental analysis : C,gH4gN3041/2CHCL 37 1/2C, HOC Hy

Calcd. : C 66.33, H 7.16, N 7.37

Found : CC 66.02, H 7.47, N 7.33 (4) 1-[2-{5-(3,4-Dihydroxyphenyl)-(2E,4E)-2,4~- pentadienoylamino}ethyl]-4- (3-indolyl) piperidine mp : 138-158°C (dec.) (recrystallized from ethanol- - } water (8:2 V/V))

IR (Nujol) : 3400, 3350, 1650, 1585, 1520 — Co

NMR (DMSO-d, §) + 1.5-3.6 (L3H, m), 6.13 (1H, 4d, : J=15Hz), 6.63-7.70 (11H, m), 7.93 (1H, m), 10.73 (1H, br) ‘

MASS (m/e) : 431 (M'), 213 (base)

Elemental analysis : ChglpgN40376/5 ethanol calcd. : C 70.07, H 7.49, N 8.63

Found : C 69.77, H 7.39, N 8.67 (5) 1-[4-{5-(4-Hydroxy-3,5-dimethoxyphenyl)-(2E,4E)- 2,4-pentadienoylamino}butyl]-4-(3-indolyl)- on ft emma Yt NL A

Cae - : IR (Nujol) : 3250, 1640, 1600, 1540, 1510, 1130, 1110, 810 cm (6) 1-[2~{5-(4-Hydroxy~3,5~dimethylphenyl)- (2E,4E)-2,4~- pentadienoylaminojethyl]-4-(3-indolyl) piperidine mp : 125-135°C (recrystallized from ethanol - water (8:2 V/V))

IR (Nujol) : 3300, 1640, 1590, 1545, 990, 860 cm *

NMR (DMSO-d, 8) : 1.4-2.4 (6H, m), 2.19 (6H, s), 2.6-3.2 (7H, m), 6.11 (1H, 4, J=1S5Hz), 6.7-7.6 (LOH, m), 7.95 (1H, m), 10.82 (1H, m) 0) MASS (m/e) : 443 (M7), 213 (base)

Elemental analysis : Coat y3N30,4/3H,50

Caled. = C 71.92, H 7.69, N 8.99

Found : CC 72.00, H 7.69, N 8.88 : (7) 1-[2-{5-(4-Hydroxy-3,5-diisopropylphenyl)-(2E,4E)- : 2,4-pentadienoylamino}ethyl]-4-(3-indolyl)piperidine mp : 110-120°C (recrystallized from ethanol - water (8:2 v/V)) oo IR (Nujol) : 3400, 3300, 1650, 1630, 1585, 995, 870 cmt

NMR (DMSO-d, §) : 1.28 (12H, d, J=8Hz), 1.5-2.4 : - : (6H, m), 2.7-3.6 (9H, m), 6.13 (1H, 4, J=15Hz), ; 9 25 6.8-7.6 (10H, m), 7.95 (1H, m), 8.4 (lH, m), : . 10.73 (1H, m)

MASS (m/e) : 499 (M7), 226, 213 (base)

Elemental analysis : Cy, H, N30, H0

Calcd. : C 74.24, H 8.37, N 8.11 : 30 Found =: C 73.84, H 8.42, N 7.97 : (8) 1-[2-{5-(4-Hydroxy-3-methylphenyl)-(2E,4E)~-2,4~ pentadienoylamino}ethyl]-4-(3-indolyl) piperidine mp : 138-141°C (recrystallized from a mixture of ! 35 ethanol - water (8:2 V/V)) .

y ——————r Co i { - 47 -

NMR (DMSO-d, 6) : 1.5-3.6 (13H, m), 2.20 (3H, s), 6.10 (1H, d, J=15Hz), 6.7-7.7 (11H, m), 7.93 (lH, m), 9.65 (1H, m), 10.73 (1H, m) © MASS (m/e) : 429 (M'), 213 (base)

Elemental analysis : C,,H3 N30, 5/4H,0 calcd. : C 71.73, H 7.47, N 9.29

Found : C 71.78, H 7.73, N 9.28 (9) 1-[2-{5-(3-Chloro-4-hydroxyphenyl)-(2E,4E)-2,4~- pentadienoylaminolethyl]-4-(3-indolyl) piperidine mp : 139-155°C (recrystallized from ethanol - water)

O IR (Nujol) : 3420, 1650, 1590, 1000 cm ©

NMR (DMSO-d, §) : 1.5-3.5 (13H, m), 6.12 (lH, 4d,

J=15Hz), 6.7-7.7 (11H, m), 7.98 (lH, m), 10.7 (1H, m)

MASS (m/e) : 449 (M'), 213 (base)

Elemental analysis : C,gHygClN30,1.5H,0

Caled. : C 65.47, H f.35, N 8.81

Found : C 65.88, H 6.44, N 8.78 : (10) 1-[2-{5-(3,5-Dichloro-4-hydroxyphenyl)- (2E,4E)- 2,4-pentadienoylamino}ethyl]-4-(3-indolyl)piperidine mp : 165-175°C (recrystallized from N,N-dimethyl- formamide) }

O 25 NMR (DMSO-d, §) + 1.5-3.6 (13H, m), 5.3 (1H, m), 6.08 (1H, 4d, J=15Hz), 6.6-7.6 (L0H, m), 8.09 (1H, m), 10.75 (lH, s)

MASS (m/e) : 485 (M+2), 483 (M'), 213 (base) (11) 1-[2-{5-(2-Hydroxy-3-methoxyphenyl)-(2E,4E)-2,4- pentadienoylaminolethyl]-4-(3-indolyl)piperidine mp : 184-186°C (recrystallized from ethanol)

IR (Nujol) : 3400, 3240, 1650, 1605, 1600, 1530, 1090, 1005 cm

NMR (DMSO-d, §) + 1.4-3.6 (13H, m), 3.78 (3H, s),

; : 6.11 (1H, 4, J=15Hz), 6.6-7.65 (11H, m), : 7.90 (1H, t like), 8.95 (lH, br), 10.75 - (1H, s) © MASS (m/e) : 445 (MT), 226, 213 (base) (12) 1-[2-{5-(3-Hydroxy—-4-methoxyphenyl)-(2E,4E)-2,4~ ; pentadienoylamino}ethyl]-4-(3-indolyl)piperidine mp : 135-140°C (recrystallized from ethanol) ; IR (Nujol) : 3350, 1650, 1615, 1590 pe

NMR (DMSO-d, §) : 1.4-3.5 (13H, m), 3.75 (3H, s), : 6.11 (1H, 4, J=15Hz), 6.6-7.7 (11H, m),

LO 7.91 (1H, t like), 9.0 (1H, br), 10.7 (lH, s)

MASS (m/e) : 445 (M'), 213 (base)

Example 8

To a mixture of 1-[2-{5-(4-hydroxy-3-methoxyphenyl)- (2E,4E)-2,4-pentadienoylamino}ethyl]-4-(3-indolyl)- piperidine (5.89 g), dry N-methylmorpholine (1.0 g) and dry N,N-dimethylformamide (10 ml) was added slowly acetyl chloride (0.26 g) at 5 to 10°C. After stirring for 1 hour, the reaction mixture was poured into water (50 ml) and stirred for 1 hour. The resulting precipitate was collected, washed with water and then recrystallized oO from a mixture of ethanol and water (7:3 V/V) to give : 25 1-[2-{5-(4~acetoxy-3-methoxyphenyl)- (2E,4E)-2,4-penta-

To dienoylamino}ethyll}-4-(3-indolyl)piperidine (0.22 g). : mp : 101-105°C (recrystallized from ethanol - water , ’ (8:2, vV/V))

IR (Nujol) : 3440, 3250, 1760, 1655, 1620, 1560, 1505 cmt

NMR (DMSO-d , §) : 1.5-2.4 (6H, m), 2.24 (3H, s), 2.6-3.5 (78, m), 3.81 (3H, s), 6.20 (lH, 4,

J=15Hz), 6.8-7.7 (11H, m), 8.04 (1H, m), 10.73 (lH, s) : 35 MASS (m/e) : 487 (M'), 213 (base)

i

Elemental analysis : C,gHy3N30, "HHO

Calcd. : C 68.89, H 6.98, N 8.31

Found : C 68.91, H 6.95, N 8.32 : 5 Example 9 : 1- [2-{5- (3-Methoxy-4-propionyloxyphenyl)- (2E, 4E)- i 2,4-pentadienoylaminolethyl]-4-(3-indolyl)piperidine was obtained according to a similar manner to that of : Example 8. mp : 157-158°C (recrystallized from ethanol)

IR (Nujol) : 3430, 3250, 3060, 1750. 1655, 1620, ; oO 1560 —

NMR (DMSO-d, §) : 1.15 (3H, t, J=8Hz), 1.5-2.4 (6H, m), 2.62 (2H, gq, J=8Hz), 2.4-3.2 (SH, m), : 15 3.33 (2H, m), 3.82 (3H, s), 6.22 (lH, 4d,

J=15Hz), 6.8-7.7 (11H, m), 8.05 (1H, m), 10.75 (1H, s)

MASS (m/e) : 501 (M'), 213 (base)

Elemental analysis : C3oH35N30, HyO

Calcd. : CC 69.34, H 7.18, N 8.09 : Found : C 69.14, H 7.09, N 8.06

Example 10

O To a mixture of I-[2-{5-(4-hydroxy-3,5-dimethoxy- phenyl) - (2E,4E)-2,4-pentadienoylamino}ethyl]-4-(3- indolyl) piperidine (1 g) and pyridine (10 ml) was added

Ls slowly acetyl chloride (0.48 ml) at 5 to 10°C. After 1 hour, the reaction mixture was poured into ice-water and extracted with ethyl acetate. The extract was : 30 washed with a saturated aqueous solution of sodium : chloride and dried over magnesium sulfate. The solvent ‘ was distilled off and the residue was subjected to column chromatography on silica gel and eluted with a ; mixture of chloroform and methanol (10:1 V/V). The fractions containing the object compound were combined and concentrated under reduced pressure. The residue was treated with a mixture of fumaric acid (83 mg) and methanol (8 ml) and concentrated under reduced pressure to give white crystals. The crystals were recrystallized from ethanol to give 1-[2-{5-(4-acetoxy-3,5-dimethoxy- phenyl) - (2E,4E)~-2,4-pentadienoylamino}tethyl]-4-(3- indolyl)piperidine 1/2fumarate (0.25 gq). ! mp : 202-209°C

IR (Nujol) : 3400, 1750, 1680, 1615, 1595, 1565 em 1

NMR (DMSO-d, §) + 1.6-2.15 (5H, m), 2.32 (3H, s), 2.2-3.6 (8H, m), 4.82 (6H, s), 6.22 (lH, 4,

O J=14Hz), 6.64 (1H, s), 6.7-7.7 (10H, m), 8.29 (1H, m), 10.75 (1H, s)

MASS (m/e) : 517 (MT), 213 (base)

Elemental analysis : CoH 4g 30" 1/2Fumarate - 3/2H,0

Calcd. : C 63.77, H 6.68, N 6.97

Found : C 63.57, H 6.44, N 6.95

Example 11 1-{2-{5-(3,5-Dimethoxy-4-propionyloxyphenyl)- (2E, 4E)- 2,4-pentadienoylaminolethyl]-4~(3-indolyl) piperidine 1/2fumarate was obtained according to a similar manner to that of Example 10. ~ mp : 188-192°C (recrystallized from ethanol) © 25 IR (Nujol) : 3400, 1745, 1680, 1615, 1595, 1565 em” 1

NMR (DMSO-d¢, 6) : 1.13 (3H, t, J=7Hz), 1.6-2.2 . (3H, m), 2.2-3.7 (12H, m), 3.81 (6H, s), : 6.21 (1H, 4, J=15Hz), 6.62 (lH, s), 6.8-7.6 (10H, m), 8.3 (1H, m), 10.78 (lH, s)

MASS (m/e) : 531 (M'), 213 (base)

Elemental analysis : Cy, HNO," 1/2Fumarate - 3/2H,0 : Calcd. : CC 64.27, H 6.86, N 6.81

Found : C 64.17, H 6.78, N 6.78 i 35

. * 1 ] Cs -

Example 12

To a mixture of 1-[2-{5-(4-hydroxy-3,5-dimethoxy- phenyl) -(2E,4E)-2,4-pentadienoylamino}tethyl]-4-(3-indolyl)- piperidine (1.19 g), triethylamine (1.74 ml) and dry : 5 N,N-dimethyl formamide (12 ml) was added slowly a mixture . of ethyl chloroformate (0.33 g) and methylene chloride : (0.5 ml) at 0 to 5°C. Similar work up gave 1-[2-{5-(4- ethoxycarbonyloxy-3,5-dimethoxyphenyl)-(2E,4E)-2,4- pentadienoylamino}ethyl}-4-(3-indolyl)piperidine (0.74 g). i 10 mp : 90-98°C (recrystallized from ethanol - water

A (8:2 V/V)) () IR (Nujol) : 3360, 3300, 1750, 1640, 1590, 1130, 1000, 735 cmt ; NMR (DMSO-d, 8) : 1.28 (3H, t, J=8Hz), 1.5-3.6 (13H, m), 3.81 (6H, s), 4.23 (2H, gq, J=8Hz), 6.21 (lH, 4, J=15Hz), 6.8-7.7 (10H, m), : 8.05 (1H, m), 10.71 (lH, s)

MASS (m/e) : Si. (M'), 228, 213 (base)

Elemental analysis : C,H; NJO."2.5H,0

Caled. : C 62.82, H 7.14, N 7.09

Found : C 62.74, H 6.93, N 7.05 : Example 13

O - The following compounds were obtained according to i 25 a similar manner to that of Example 12. i : (1) 1-[4-{5-(4-Ethoxycarbonyloxy-3,5-dimethoxyphenyl)- . (2E,4E)-2, 4-pentadienoylaminol}tbutyl]-4-(3-indolyl)- piperidine , 30 mp : 90-98°C (recrystallized from ethanol - water

J } (8:2 vV/V))

IR (Nujol) : 3380, 3250, 1750, 1655, 1620, 1595, 1555, 1130, 1050, 1000, 735 cm © ; NMR (DMSO-d, 6) : 1.27 (3H, t, J=8Hz), 1.4-3.7 : 35 (17H, m), 3.72 (6H, s), 4.23 (2H, gq, J=8Hz), {

- 52 ~- 6.20 (1H, 4, J=15Hz), 6.8-7.75 (10H, m) , 8.10 (1H, m), 10.76 (1H, s)

MASS (m/e) : 575 (M'), 531, 503, 285, 233, 213 (base)

Elemental analysis : C 43H, N40" 3/2ethanol

Calcd. : C 67.01, H 7.81, N 6.52

Found : CC 66.39, H 7.74, N 6.52 (2) 1-({4-{5-(3,5-Dimethoxy-4-propionyloxyphenyl)- (2E,4E)~ 2,4-pentadienoylamino}butyl]-4-(3-indolyl)piperidine hydrochloride

O mp : 215-220°C (recrystallized from acetonitrile)

IR (Nujol) : 3250, 2650, 2500, 1760, 1650, 15135, 1130, 1010, 850, 750 cm *

NMR (CDC1 4, §) «+ 1.29 (3H, t, J=8Hz), 2.65 (2H, q,

J=8Hz), 1.5-3.7 (174, m), 3.80 (6H, s), 6.35 (lH, 4, J=15Hz), 6.6-7.7 (10H, m), 7.9 (1H, m), 9.05 (1H, m), 11.3 (1H, m)

MASS (m/e) : 559 (M'), 503, 233, 213 (base) (3) 1-[2-[5-{3,4-bis (Ethoxycarbonyloxy) phenyl} -(2E, 4E) - 2,4-pentadienoylamino]ethyl]-4-(3-indolyl)piperidine mp : 135-137°C (recrystallized from a mixture of . water and ethanol)

O 25 IR (Nujol) : 3500, 3350, 1775, 1650, 1620, 1000 a

NMR (DMSO-d., §) : 1.30 (6H, t, J=8Hz), 1.3-3.5 (13H, m), 4.30 (4H, gq, J=8Hz), 6.25 (lH, 4, J=15Hz), 6.6-7.7 (11H, m), 8.08 (1H, m), 10.73 (1H, s)

MASS (m/e) : 529 (M'-46), 457, 285 (base), 213

Example 14

To a mixture of 1-[2-{5-(4-hydroxy-3,5-dimethoxy- phenyl) - (2E,4E)-2,4-pentadienoylaminolethyl]-4-(3-indolyl)- piperidine (2.0 g), triethylamine (2.9 ml) and dry N,N- ~ dimethylformamide (20 ml) was added slowly a solution of acetylchloride (0.5 g) in methylene chloride (1.0 ml)

} ! - 53 - at 0 to 5°C. After 1 hour, the reaction mixture was poured into water (200 ml) and stirred for 1 hour. The resulting precipitate was collected, washed with water and air-dried at ambient temperature. The precipitate was subjected to column chromatography on silica gel ; (60 g) and eluted with a mixture of chloroform and methanol (20:1 V/V). The fractions containing the object compound were combined and concentrated under reduced pressure. The residue was recrystallized from ! 10 ethyl acetate to give pale yellow crystals of 1-[2-{5-(4- acetoxy-3, 5-dimethoxyphanyl) - (2E, 4E) -2, 4-pentadienoylamino}- ethyl]-4-(3-indolyl)piperidine (1.35 g).

Lo mp : 169-172°C

IR (Nujol) : 3380, 3320, 1755, 1650, 1620, 1595, 990, 745 cm T

NMR (CDCl, §) + 1.5-3.6 (13H, m), 2.32 (3H, s), 3.82 (6H, s), 6.0 (1H, 4, J=15Hz), 6.34 (lH, m), 6.7-7.7 (LOH, m), 8.32 (1H, m)

MASS (m/e) : 517 (M%), 213 (base)

Elemental analysis : C4, HNO,

Calcd. : C 69.61, H 6.82, N 8.12

Found : C 69.35, H 6.82, N 8.02

Example 15 | ”

To a stirred mixture of 5-[3,5-dimethoxy-4- {(2-methoxyethoxy)methoxy}phenyl]-(2E,4E)-2,4-pentadienoic : acid (1.35 g) and triethylamine (1.17 ml) in dry N,N- dimethylformamide (8 ml) was added slowly diphenyl phosphinic chloride (0.97 g) at -10 to -15°C under an inert atmosphere. After being stirred for 1 hour, a solution of 1-(2-aminoethyl)-4-(3-indolyl)piperidine (0.97 gq) in dry

N,N-dimethylformamide (8 ml) was added slowly to the reaction mixture at the same temperature. After being stirred for 40 minutes at the same temperature, the reaction mixture was poured into ice-water (160 ml) and extracted

; - - 54 i with ethyl acetate. The extract was washed with a saturated i sodium chloride solution and dried over magnesium sulfate. ; The solvent was evaporated to give syrup of 1-{2-(5-[3,5- dimethoxy-4-{(2-methoxyethoxy)methoxy}phenyl]-(2E,4E)~-2,4- pentadienoylamino]ethyl]-4-(3-indolyl)piperidine (1.97 g).

IR(Nujol): 3300, 1650, 1610, 1580, 1125, 990, 960, 845, 745 cm © i 10 : oo :

Claims (8)

1. A compound of the formula : g ( N-A-NUCO-B-R! N H wherein Rl [EE phenyl auhastitnted with aubhsat i Fnent (a) selected from the group consisting of lower alkyl, hydroxy, protected hydroxy, halogen and lower alkoxy, A ig lower alkylene, and R igs lower alkenvlene, and a pharmaceutically accepltahle salt thereof,

2. A compound of claim 1, wherein Rr! is phenyl substituted with enhslituent (x) selected from the group cansisting of lower alkyl, hydroxy, lower alkoxy(lower)alkaxy(lower)alkoxy, acyloxy, halogen and lower alkoxy.

3. A compound of claim 2, wherein r! is phenyl snhstituted with substituent (sg) selected from the group consisting of lower alkyl, hydroxy, lower alkanovlnxy, lower alkoxycarbonyloxy, halogen and lower alkoxy.

4. A compound of claim 3, wherein rR! is phenyl substituted with mono-, or dihydroxy and mono-, or di{lower)alkoxy. BAD ORiune J

; . pentadienaylamino}ethyl ]-4-(3-indolyl piperidine.

6. A compound of claim 3, wherein rl is phenyl suhstitnted with mono-, or di(lower)- alkanoyloxy and mono-, or di{lower)alkoxy, or with mona-, ov di(lowver)alkoxycarbonyloxy and mono-, or di(lower)alkoxy.

7, A campound of e¢laim 6, which is 1-[2-{A-(4-acetoxy-3,6-dimethoxyphenyl)}-(2F,4E)-2,4~ pentadienoylamino}lethyl]-4-{(2~indolyl}piperidine.

8. A compound of claim 6, which is 1-[2-{h-(4-ethoxyoarhonyloxy- 3,5-dimethoxyphenyl)-(2E,4R)-2,4-pentadienoylamino}ethyl ]-4- (3~indolyl)-piperidine, 9, A pharmaceutical composition comprising a compound of claim 1, as an active ingredient, in association with a pharmaceutically acceptable, nontoxic carrier or excipient. 10, A method for the therapeutic treatment of allergic disease which comprises administering an effective amount of a compound of claim 1 in human heings or animals, Inventors: MASAAKI MATSUO TAKASHT MANARE SHINJI SHIGENAGA HTIROSHT MATSUDA -3 BAD ORIGINAL {oi Rtn aces.